Download low species diversity

PURPOSE
A: to discover the variety of bacteria on Westwood’s
campus and on the human body
B: to study the growth of organisms in a closed system
INOCULATE
to introduce new cells or organisms into a culture medium
CULTURE MEDIUM
a liquid or gelatinous substance used to grow cells
LB AGAR
lysogeny broth; a nutrient-rich culture used primarily for
bacterial growth
BIODIVERSITY
• a measure of variation in genetics, species, or
ecosystems
• Antarctica has low species diversity while the
Amazon rainforest has high species diversity
BIODIVERSITY
• Cheetahs have low genetic diversity while
leopards have high genetic diversity
PROCEDURES
1.
Obtain two Petri dishes. Section each Petri dish into
four even quadrants.
2. As a class, vote for four locations on Westwood’s
campus to study bacterial variation.
Vote for four locations on the human body to study
bacterial variation.
PROCEDURES
4. Using a sterile pipette, wet the end of a cotton swab
with distilled water.
5. Using a microtube, secure the cotton swab and take it
to the sample location.
6. In a swirling motion, rub the cotton swab on the
sample location.
7.
Using a microtube, secure the cotton swab and return
to the classroom
PROCEDURES
8. In a swirling motion, inoculate the quadrant of the Petri
dish with the cotton swab.
9. Repeat steps 4-8 for each of the eight total sample
locations.
10. Store the Petri dishes in a closed environment and
leave undisturbed at 37 °C.
11. Record observations on the data tables at 24 hours and
at 48 hours after inoculation.