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9-2 The Krebs Cycle and Electron Transport Slide 1 of 37 Copyright Pearson Prentice Hall End Show 9-2 The Krebs Cycle and Electron Transport 9-2 The Krebs Cycle and Electron Transport Glycolysis-takes place cytoplasm. 2 ATP molecules used to split glucose; 4 ATP (2 ATP net) and 2 NADH formed as the 3-carbon molecules are rearranged into 2 molecules of pyruvate. Oxygen is required for the final steps of cellular respiration. Because the pathways of cellular respiration require oxygen, they are aerobic. Slide 2 of 37 Copyright Pearson Prentice Hall End Show 9-2 The Krebs Cycle and Electron Transport The Krebs Cycle What happens during the Krebs cycle? Slide 3 of 37 Copyright Pearson Prentice Hall End Show 9-2 The Krebs Cycle and Electron Transport The Krebs Cycle During the Krebs cycle, pyruvic acid is broken down into carbon dioxide in a series of energy-extracting reactions. Slide 4 of 37 Copyright Pearson Prentice Hall End Show 9-2 The Krebs Cycle and Electron Transport The Krebs Cycle The Krebs cycle begins when pyruvic acid produced by glycolysis enters the mitochondrion. Slide 5 of 37 Copyright Pearson Prentice Hall End Show 9-2 The Krebs Cycle and Electron Transport The Krebs Cycle One carbon molecule is removed, forming CO2, and electrons are removed, changing NAD+ to NADH. Slide 6 of 37 Copyright Pearson Prentice Hall End Show 9-2 The Krebs Cycle and Electron Transport The Krebs Cycle Coenzyme A joins the 2-carbon molecule, forming acetyl-CoA. Slide 7 of 37 Copyright Pearson Prentice Hall End Show 9-2 The Krebs Cycle and Electron Transport The Krebs Cycle Acetyl-CoA then adds the 2-carbon acetyl group to a 4carbon compound, forming citric acid. Citric acid Slide 8 of 37 Copyright Pearson Prentice Hall End Show 9-2 The Krebs Cycle and Electron Transport The Krebs Cycle Citric acid is broken down into a 5-carbon compound, then into a 4-carbon compound. Slide 9 of 37 Copyright Pearson Prentice Hall End Show 9-2 The Krebs Cycle and Electron Transport The Krebs Cycle Two more molecules of CO2 are released and electrons join NAD+ and FAD, forming NADH and FADH2 Slide 10 of 37 Copyright Pearson Prentice Hall End Show 9-2 The Krebs Cycle and Electron Transport The Krebs Cycle In addition, one molecule of ATP is generated. Slide 11 of 37 Copyright Pearson Prentice Hall End Show 9-2 The Krebs Cycle and Electron Transport The Krebs Cycle The energy tally from 1 molecule of pyruvic acid is • • • 4 NADH 1 FADH2 1 ATP Slide 12 of 37 Copyright Pearson Prentice Hall End Show 9-2 The Krebs Cycle and Electron Transport Krebs Cycle or Citric Acid Cycle 1. Pyruvate broken down; carbon dioxide is released. 2. Coenzyme A binds; intermediate enters Krebs Cycle 3. Citric acid (6 carbon molecule) is formed. 4. Citric acid broken down; NADH is made; carbon dioxide is released. 5. 5-carbon molecule broken down; NADH and ATP made; carbon dioxide is released. 6. 4-carbon molecule rearranged, NADH and FADH2 made. Slide 13 of 37 End Show 9-2 The Krebs Cycle and Electron Transport Electron Transport How are high-energy electrons used by the electron transport chain? Slide 14 of 37 Copyright Pearson Prentice Hall End Show 9-2 The Krebs Cycle and Electron Transport Electron Transport Electron Transport The electron transport chain uses the highenergy electrons from the Krebs cycle to convert ADP into ATP. Slide 15 of 37 Copyright Pearson Prentice Hall End Show 9-2 The Krebs Cycle and Electron Transport Electron Transport High-energy electrons from NADH and FADH2 are passed along the electron transport chain from one carrier protein to the next. Slide 16 of 37 Copyright Pearson Prentice Hall End Show 9-2 The Krebs Cycle and Electron Transport Electron Transport At the end of the chain, an enzyme combines these electrons with hydrogen ions and oxygen to form water. Slide 17 of 37 Copyright Pearson Prentice Hall End Show 9-2 The Krebs Cycle and Electron Transport Electron Transport As the final electron acceptor of the electron transport chain, oxygen gets rid of the low-energy electrons and hydrogen ions. Slide 18 of 37 Copyright Pearson Prentice Hall End Show 9-2 The Krebs Cycle and Electron Transport Electron Transport When 2 high-energy electrons move down the electron transport chain, their energy is used to move hydrogen ions (H+) across the membrane. Slide 19 of 37 Copyright Pearson Prentice Hall End Show 9-2 The Krebs Cycle and Electron Transport Electron Transport During electron transport, H+ ions build up in the intermembrane space, so it is positively charged. Slide 20 of 37 Copyright Pearson Prentice Hall End Show 9-2 The Krebs Cycle and Electron Transport Electron Transport The other side of the membrane, from which those H+ ions are taken, is now negatively charged. Slide 21 of 37 Copyright Pearson Prentice Hall End Show 9-2 The Krebs Cycle and Electron Transport Electron Transport The inner membranes of the mitochondria contain protein spheres called ATP synthases. ATP synthase Slide 22 of 37 Copyright Pearson Prentice Hall End Show 9-2 The Krebs Cycle and Electron Transport Electron Transport As H+ ions escape through channels into these proteins, the ATP synthase spins. Channel ATP synthase Slide 23 of 37 Copyright Pearson Prentice Hall End Show 9-2 The Krebs Cycle and Electron Transport Electron Transport As it rotates, the enzyme grabs a low-energy ADP, attaching a phosphate, forming high-energy ATP. Channel ATP synthase ATP Slide 24 of 37 Copyright Pearson Prentice Hall End Show 9-2 The Krebs Cycle and Electron Transport Electron Transport Chain 1. Energized electrons removed from NADH and FADH2 2. Energy from electrons in the ETC is used to pump H+ ions across the inner mitochondrial membrane 3. H+ ions flow through ATP synthase, and ATP molecules are produced 4. Oxygen picks up electrons that went through the ETC and H+ ions Slide 25 of 37 End Show 9-2 The Krebs Cycle and Electron Transport The Totals The Totals Glycolysis produces just 2 ATP molecules per molecule of glucose. The complete breakdown of glucose through cellular respiration, including glycolysis, results in the production of 36 molecules of ATP. Slide 26 of 37 Copyright Pearson Prentice Hall End Show 9-2 The Krebs Cycle and Electron Transport The Totals Slide 27 of 37 Copyright Pearson Prentice Hall End Show 9-2 The Krebs Cycle and Electron Transport Comparing Photosynthesis and Cellular Respiration Comparing Photosynthesis and Cellular Respiration The energy flows in photosynthesis and cellular respiration take place in opposite directions. Slide 28 of 37 Copyright Pearson Prentice Hall End Show 9-2 The Krebs Cycle and Electron Transport Comparing Photosynthesis and Cellular Respiration On a global level, photosynthesis and cellular respiration are also opposites. • Photosynthesis removes carbon dioxide from the atmosphere and cellular respiration puts it back. • Photosynthesis releases oxygen into the atmosphere and cellular respiration uses that oxygen to release energy from food. Slide 29 of 37 Copyright Pearson Prentice Hall End Show 9-2 Click to Launch: Continue to: - or - Slide 30 of 37 End Show Copyright Pearson Prentice Hall 9-2 The Krebs cycle breaks pyruvic acid down into a. oxygen. b. NADH. c. carbon dioxide. d. alcohol. Slide 31 of 37 End Show Copyright Pearson Prentice Hall 9-2 What role does the Krebs cycle play in the cell? a. It breaks down glucose and releases its stored energy. b. It releases energy from molecules formed during glycolysis. c. It combines carbon dioxide and water into high-energy molecules. d. It breaks down ATP and NADH, releasing stored energy. Slide 32 of 37 End Show Copyright Pearson Prentice Hall 9-2 In eukaryotes, the electron transport chain is located in the a. cell membrane. b. inner mitochondrial membrane. c. cytoplasm. d. outer mitochondrial membrane. Slide 33 of 37 End Show Copyright Pearson Prentice Hall 9-2 To generate energy over long periods, the body must use a. stored ATP. b. lactic acid fermentation. c. cellular respiration. d. glycolysis. Slide 34 of 37 End Show Copyright Pearson Prentice Hall 9-2 Which statement correctly describes photosynthesis and cellular respiration? a. Photosynthesis releases energy, while cellular respiration stores energy. b. Photosynthesis and cellular respiration use the same raw materials. c. Cellular respiration releases energy, while photosynthesis stores energy. d. Cellular respiration and photosynthesis produce the same products. Slide 35 of 37 End Show Copyright Pearson Prentice Hall END OF SECTION