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Electrolytes Biology Electrolytes • Free moving ions in a solution that conduct electricity – Galvanic cell converts chemical energy into electrical – Electrolytic cell – converts electrical energy into chemical Biological concepts related to ions • Fluid balance • Photosynthesis/ respiration – Electron transport system • Membrane potential • Oxidation reduction reactions Terms • Flow of electrons (ions) from one region to another is driven by a difference in potential energy • Voltage: difference in electrochemical potential energy between two electrodes (V) – Does not depend on size of the electrodes or amount of materials. AAA, AA, A all have 1.54 V – All our cells have -90mV • Current: the rate of electron flow (amps) Major electrolytes • Taken as minerals (along with vitamins) • Several of them are needed for proper function of enzymes (cofactors) • As for your body, the major electrolytes are as follows: – – – – – – – – sodium (Na+) - depolarization potassium (K+) - repolarization chloride (Cl-) – balancing the charges calcium (Ca2+) – trigger muscle contraction magnesium (Mg2+) bicarbonate (HCO3-) – acid/base phosphate (PO42-) - bones sulfate (SO42-) - bones Electrolytes and Water Balance • Pure water vs electrolytes • cells need to be bathed in fluids — inside and out. • the current enables electrolytes to regulate how and where fluids are distributed throughout the body, which includes keeping water from floating freely across cell membranes. – Water follows the charged ions • help maintain a state of fluid balance. • transport nutrients into cells and wastes out of them. • The difference in electrical balance inside and outside of cells also allows: – – – – for transmission of nerve impulses, contraction or relaxation of muscles, blood pressure control, and proper gland functioning. Sodium levels • 136 and 145 milliequivalents per liter (mEq/L) of sodium • Sodium plays a key role in your body. It helps maintain normal blood pressure, supports the work of your nerves and muscles, and regulates your body's fluid balance. • Hyponatremia: When the sodium levels in your blood become too low, excess water enters your cells and causes them to swell. Swelling in your brain is especially dangerous because the brain is confined by your skull and unable to expand. • Hypernatremia, the body contains too little water for the amount of sodium. The sodium level in blood becomes abnormally high when water loss exceeds sodium loss, as typically occurs in dehydration, typically causes thirst. The most serious symptoms of hypernatremia result from brain dysfunction, confusion, muscle twitching, seizures, coma, and death. Water and Mineral Availability Only minerals dissolved in water in spaces among soil particles are available -Organic soil particles tend to have negative charges, and so attract positive ions -Therefore, active transport is needed to move positive ions into root hairs 8 Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display. 1. Soil particles tend to + – + have a negative charge. + + – – + – – – Soil particle + – – – + – – + – ATP – – + – + + – + – 2. Positive ions are attracted to soil particles. + + 3. Negative ions stay in + – – – Root hair + + solution surrounding roots, creating a charge gradient that tends to “pull” positive ions out off the root cells. + 4. Active transport is Water required to acquire and maintain K+ and other positive ions in the root. 9 Plant Nutrients Plants require a number of inorganic nutrients -Macronutrients: Used in relatively large amounts -Nine = C, O, H, N, K, Ca, Mg, P & S -Micronutrients: Used in minute amounts -Seven = Cl, Fe, Mn, Zn, B, Cu & Mo A deficiency of any one can have severe effects on plant growth 10 Plant Nutrients 11 Responses to Mechanical Stimuli http://plantsinmotion.bio.indiana.edu/plantmotion/m ovements/nastic/mimosa/mimosa.html (animation of plant response) Mimosa pudica leaves have swollen structures called pulvini at the base of their leaflets -When leaves are stimulated, an electrical signal is generated -Triggers movement of ions to outer side of pulvini -Water follows by osmosis -Decreased interior turgor pressure causes the leaf to fold 12 Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display. Leaflet blade Pulvinus Vascular tissue Petiole a. Cells gaining turgor b. Cells losing turgor Cl– K+ H2 O 13 Carnivorous Plants Often grow in acidic soils that lack nitrogen -Trap and digest small animals, primarily insects, to extract additional nutrients -Have modified leaves adapted for luring and trapping prey -Prey is digested with enzymes secreted from specialized glands 14 15 The Rate of Transpiration Guard cells have thicker cell walls on the inside and thinner cell walls elsewhere -This allows them to bulge and bow outward when they become turgid -Causing the stomata to open Turgor in guard cells results from the active uptake of potassium (K+), chloride (Cl–), and malate 16 Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display. Tied end Slightly inflated balloon “Stoma” Add turgor pressure (air) Add thickened inner walls (overlapping duct tape) 17 Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display. Closed stoma: flaccid guard cells Little water in vacuole Open stoma: turgid guard cells H2O K+ Malate2– Cl– H2O H2O Vacuole filled with water 18 The Rate of Transpiration Closed stoma: flaccid guard cells Open stoma: turgid guard cells H2O K+ H2O Cl– H2O Malate2– H2O K+ H2O Cl– H2O Malate2– ABA K+ K+ H2O H2O Cl– Cl– H2O H2O ABA Malate2– ABA K+ H2O Cytosol H2O Cl– Malate2– H2O K+ H 2O Malate2– Cytosol H2O Cl– H2O Malate2– ABA ABA 19 Water Stress Responses Plants, such as mangroves, that grow in salt water produce pneumatophores -Long, spongy, air-filled roots, that emerge above the mud -Have large lenticels through which oxygen enters -These plants also secrete large quantities of salt 20 Water Stress Responses 21 Water Stress Responses Plants called halophytes live in saline soil -Produce high concentrations of organic molecules in their roots -This decreases the water potential enhancing water uptake from the soil 22 Humans and Electric shock • 1mA – sense the shock • 10-20mA “knocked across the room” or “can’t let go” effect – muscle contract and propelling them • 100-300 mA – irregular uncoordinated heart beat disrupts overall pattern of the heart beat • Defibrillator – 6A – continuous ventricular contraction and respiratory paralysis • Everything stops – back to normal heart beat Frequency effect • 60 Hz – same as firing frequency of nerves • Humans are most sensitive and is most dangerous frequency Resting membrane potential • Bioelectricity – Slight differences in ion concentrations – Inside more negative – Outside more positive – Difference -80mV – Concentration gradient - electrical potential – Coulombs force Action potential • Depolarization • Repolarization Fig. 8.10 Fig. 8.9 Fig. 8.18 Fig. 12.14 Fig. 12.15 Fig. 12.16