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Chapter 7: CELLS Introduction: 1. Robert Hooke an English scientist in the early 1600’s observed cells for the first time using a compound light microscope to study cork, dead cells of oak bark. Before the invention of the microscope, no one knew about cells or about microscopic organisms like bacteria and viruses. Cell Theory 1. All organisms are composed of one or more cells. 2. The Cell is the basic functional unit of life. A single cell exhibits all 5 characteristics of life. 3. All Cells come from preexisting cells. Cells divide to form 2 identical cells. 4. Cells pass on genetic information to their Daughter cells in the form of DNA. •Cells are the smallest living units. •They are organized & specialized. Stomach Cells (Cells) Stomach Tissue Stomach (Tissue) (Organ) Digestive System (Organ System) 1. The Cells: Cells are made up of specialized parts called Organelles. Each organelle has a specific function for cell survival. 2. All the Organelles are suspended in a clear, gelatinous fluid called Cytoplasm. Major Organelles and Their Functions 1. Nucleus: controls the functions of a cell through DNARNA Protein Trait. a. Chromatin: Loose DNA inside the nucleus containing over 600,000 pages of information giving all of the directions on the structure and function of an organism. b. Nucleolus: a dark spot in the nucleus responsible for the making of Ribosomes. c. Nuclear Envelope: A semi- permeable, double Phospholipid membrane that separates the nucleus from the cytoplasm. Major Organelles and Their Functions 2. Cell Membrane: Controls what goes in an out of a cell (Homeostasis). Major Organelles and Their Functions 3. Mitochondria: Produces energy for the cell through the process of Cell Respiration. ( Power Plant) Major Organelles and Their Functions 4. Lysosomes: Breaks down worn out cell parts and large molecules that enter the cell with digestive enzymes (Garbage Truck) Major Organelles and Their Functions 5. Ribosomes: Build Proteins (Factory) Major Organelles and Their Functions 6. Endoplasmic Reticulum (ER): a Transport system between the Nucleus and the Cytoplasm. A. Rough ER: its walls are lined with Ribosomes. This organelle is folded many times to provide more surface area for Ribosomes. Function: Transports Proteins, and is a site for chemical reactions. B. Smooth ER: No Ribosomes. Function: Site for Chemical Reactions and builds Lipids Major Organelles and Their Functions 6. Endoplasmic Reticulum (ER): a transports system between the Nucleus and the Cytoplasm. Transit System ( Major Organelles and Their Functions 7. Golgi Apparatus (Golgi Body): Packages Proteins for export outside the cell. Major Organelles and Their Functions 8. Cytoskeleton: is a support structure in the cytoplasm. It forms a framework for the cell, like your skeleton forms a framework for your body. The Cytoskeleton is made of: A. Microtubules: Thin, hollow cylinders made of protein. B. Microfilaments: Thin, solid protein fibers. Major Organelles and Their Functions 9. Cilia and Flagella: are structures that aid in the locomotion and feeding of cells. They are located on the outside of some cells. They are composed of pairs of microtubules, with a central pair surrounded by 9 additional pairs. All surrounded by the Plasma membrane. Major Organelles and Their Functions 9. Cilia and Flagella: Major Organelles and Their Functions A. Cilia: Short, numerous, hair-like projections that move in a wave like motion Major Organelles and Their Functions 9. Cilia: Major Organelles and Their Functions B. Flagella: are longer projections that move with a whip-like motion. Major Organelles and Their Functions 9.Flagella: Major Organelles and Their Functions 9.Flagella: Major Organelles and Their Functions Example of How Cell Organelles Work Together The Production and Exporting of Protien 1. Nucleolus- build ribosomes 2. Nucleus – sends instructions to the ribosomes 3. Ribosomes make protein 4. ER- Transports protein to Golgi 5. Golgi – Packages protein 6. Protein- Leaves the cell through the Cell Membrane. Cell Part Function City Counter Part I. EUKARYOTES Vs PROKARYOTES 2 types of cells 1.Prokaryotes: Don’t contain membrane bound organelles or nucleus. They are the most primitive cells. Ex. Bacteria. All prokaryotes are unicellular organisms. 2. Eukaryotes: Contain membrane bound organelles and a Nucleus. Organelles are separated into distinct compartments. Ex. Some unicellular organisms such as algae and yeast. + all multicellular organisms are Eukaryotes. Characteristics Prokaryotes Eukaryotes DNA Loose in the Organized as Cytoplasm, chromatin in a organized in nucleus a single ring. Organelles Contains ribosomes but no other membrane bound organelle. Life Forms Unicellular Both unicellular Ex. Bacteria AND Multicellular Contains ribosomes and many organelles Two Types of Eukaryotic cells: Animals and Plant Cells: Both have all the organelles we have talked about. There are only a few differences. Plants have the following: 1. Cell Wall: An inflexible barrier that protects plants and some prokaryote cells and gives it support. Plant cell wall is made up of the Carbohydrate called Cellulose. 2. Vacuoles: Membrane bound sacs used for storage of food, enzymes, water, and waste products. Plant cells have much larger vacuoles than animal cells. 3. Chloroplasts: organelles that capture light energy food to store for a later time. Photosynthesis A. Chlorophyll: The pigment in chloroplasts that traps light energy and gives leaves and stems their green color. 4. Animal cells contain Centrioles, a structure that plays a part in cell division. Plants don’t have Centrioles. Animal Plant Cells Cells Contains a cell wall No Cell wall Chloroplasts No Chloroplasts Large Vacuoles Small Vacuoles Nucleus+ all the Nucleus+ all organelles we talked the about before except no organelles we Centrioles talked about before + Centrioles CELL MEMBRANE AND CELLULAR TRANSPORT 1. Plasma or Cell Membrane: maintains the cell’s internal environment, maintaining Homeostasis. 2. HOW? The plasma membrane is Selectively Permeable: it allows some molecules into the cell while keeping others out. Ex. A screen in a window. 3. Structure: The plasma membrane is composed of a Phospholipids Bilayer, 2 layers of phospholipids back to back. CELL MEMBRANE AND CELLULAR TRANSPORT Phospholipid: A lipid with a phosphate group attached to it. **Remember: Polar molecules attract polar molecules. Polar attracts polar. Polar repels non-polar. *** Since Water is polar and is located both on the inside and on the outside of cells: The non-polar tails hide from the water but also act as a barrier to it and to other polar molecules. CELL MEMBRANE AND CELLULAR TRANSPORT Fluid Mosaic Model: The plasma membrane is flexible. Phospholipid molecules move within the membrane. *Cholesterol: prevents the fatty acid tails from sticking together. As a result, it helps the membrane’s fluidity. CELL MEMBRANE AND CELLULAR TRANSPORT **Transport Proteins: Proteins imbedded in the membrane that span the membrane like floating tunnels connecting the inside and the outside environments of the cell. They open up at times to allow needed molecules in and waste out. CELL MEMBRANE AND CELLULAR TRANSPORT CELL MEMBRANE AND CELLULAR TRANSPORT ***Marker Proteins: Proteins on the outer surface of the Membrane. They help cells identify each other like a uniform. It prevents your immune system from attacking your cells. Ex. Blood types and Bacteria. CELL MEMBRANE AND CELLULAR TRANSPORT ****Receptor Proteins: Proteins on the outer surface of the cell membrane that act as a lock for transport proteins. This helps our body control what goes in and out of cells with the help of hormones (endocrine system). Hormones act as a key for the locks so cells will let certain substances into cells when needed. Ex. Insulin / Diabetes example. Chapter 8.1 : Cellular Transport In 1827, Scottish scientist, Robert Brown observed what was later called Brownian motion. 1. Brownian Motion: The random movement of Particles. All Molecules, solid, liquid, and gas have kinetic energy, the energy of motion, and are constantly moving. The result of Brownian Motion is Diffusion. Diffusion: the net movement of particles from an area of higher concentration to an area of lower concentration. Ex. If you spray cologne into a room, the particles of the fragrance will move from its concentrated spray (Higher Concentration) to the rest of the room (Lower Concentration) until the particles are distributed evenly throughout the room. Diffusion is a slow process because it depends on random movements of particles. There are 3 factors that affect its speed: A. Concentration: the more concentrated the substance, the more rapidly diffusion occurs. B. Temperature: increased temperature = Molecular Movement = increased rate of Diffusion Pressure: increased Pressure = Molecular Movement = increased rate of Diffusion. Diffusion in Living Systems Concentration Gradient: The difference in concentration of a substance across space. There are concentration gradients between the water solutions outside cells and inside cells. Outside Cell Inside Cell 90% 50% Water Water 10% 50% Other Other Substances will move with the concentration gradient, from high concentration, to low concentration until a Dynamic Equilibrium is reached. Dynamic Equilibrium: When materials moving out of the cell = materials moving inside the cell. Human Example: Oxygen diffuses into the capillaries of the lungs because there is a greater concentration of Oxygen in the Air Sacs of the lungs than in the Capillaries. Osmosis: the diffusion of water across a selectively permeable membrane. It is an important of Homeostasis. Isotonic Solution: There is an equal amount of dissolved substances inside and outside the cell. As a result, the concentration of water is the same inside and outside the cell. – No net movement of water = Dynamic Equilibrium. B. Hypotonic Solution: The concentration of water is greater outside the cell that inside. As a result, water goes from the higher concentration outside the cell to the lower concentration inside the cell. C. Hypertonic Solution: The concentration of water is greater inside the cell than outside the cell. As a result, water would go from the higher concentration inside the cell to the lower concentration outside the cell. Real Life Example: Bacteria will not grow on fruit preserves because when they land on the sugar filled preserves, the bacteria shrivel up loosing water weight until they die. IV. TWO TYPES OF TRANSPORT 1. Passive Transport: Movement of particles across a membrane down the concentration gradient. Like going down stream in a canoe, this requires no cell energy. A. Ex. Diffusion and Osmosis B. Facilitated Diffusion: Particles that can’t go through the phospholipid bilayer or are too large to pass through can still come through the membrane with the help of transport proteins. * Transport proteins provide convenient openings for particles to pass through, requiring no energy. 2. Active Transport: Transport of particles into and out of a cell from an area of low concentration to an area of high concentration. From low to high, up the concentration gradient. Like going up stream in a boat, this V. TRANSPORT OF LARGE PARTICLES: 1. Endocytosis: (in) is a process by which a cell surrounds and takes in large materials from its environment. 2. Exocytosis: (out) The reverse process where large materials are exported from the cell.