Download genetic engineering practice test

1.
Small circular molecules of DNA in bacteria re called
a. plasmids
b. desmids c. pili
d. F particles e. transferrins
2.
Enzymes used to cut DNA molecule in recombinant DNA research are
a. ligases b. restriction enzymes c. transcriptases
d. DNA polymerases
e. replicases
3.
Plasmids
a. are self-replicating circular molecules of DNA b. are sites for inserting genes for
amplification c. may be transferred between different species of bacteria
d. may confer the ability to donate genetic material when bacteria conjugate (reproduce)
e. all of these
4.
The “natural” use of restriction enzymes by bacteria is to
a. integrate viral DNA b. destroy viral DNA c. repair “sticky ends”
genes e. clone DNA
d. copy the bacterial
5.
Restriction enzymes
a. often produce staggered cuts in DNA that are useful in splicing genes b. are like most
enzymes in being very specific in their action
c. are natural defense mechanisms evolved
in bacteria to guard against or conteract bacteriophages
d. are used along with ligase and
plasmids to produce a DNA libarary e. all of these
6.
Which of the following enzymes join the paired sticky ends of DNA fragments?
a. reverse transcriptase b. restriction enzymes c. DNA ligase d. DNA polymerase
e. transferase
7.
RNA can manufacture DNA via the action of
a. DNA polymerase b. RNA polymerase c. reverse transcriptase
d. ligase e. restriction endonucleases
8.
The laboratory technique used to separate the DNA fragments produced by automated DNA
sequencing is
a. the polymerase chain reaction b. gel electrophoresis c. ultracentrifugation
d. electron microscopy e. fluorescence microscopy
9.
What is the ultimate source of genetic variability?
a. inbreeding b. plasmids
c. hybridization
d. mutations
10.
Which of the following is a clone?
a. the adult female sheep whose DNA was used to produce Dolly b. a transgenic mouse
c. genetically identical bacteria produced from a single cell d. the tobacco plant with the
luciferase gene
11.
One function of gel electrophoresis is to
a. separate DNA fragments b. cut DNA c. recombine DNA
DNA
d. make many copies of
12.
The process of making changes in the DNA code of living organisms is called
a. selective breeding b. genetic engineering c. inbreeding d. hybridization
13.
A DNA molecule produced by combining DNA from different sources is known as
a. a mutant b. a hybrid c. a polyploid
d. recombinant DNA
14.
Knowing the sequence of an organisms DNA allows researchers to
a. clone the organism b. mutate the DNA c. study specific genes
d. extract the DNA
15.
All of the following involve the genetic engineering technique EXCEPT
a. cutting and rearranging the DNA n b. using restriction enzymes to cut specific sequences
of DNA
c. cloning the genes into the host organism
d. using any cell as a vector
16.
Vectors are vehicles of transport of foreign DNA fragments into host cells and usually are
either a bacterial plasmid or
a. a virus b. a protist c. yeast
d. a plant cell e. human cell
17.
By gel electrophoresis, fragments of DNA can be separated according to electrical charge and
___
a. sequence
b. electrical charge
c. size
d. species
18.
Which of the following would be true of a transgenic mouse?
a. it has genes easily transferred to other mice b. it has genes from some other source
c. it is a mutant
d. it is infected by some virus
e. it comes from a group of mice whose
DNA is easily manipulated
19.
Restriction enzymes work by
a. cutting DNA at specific recognition sequences b. cutting DNA at random into many
fragments
c. splicing DNA from different sources together
d. enzymes that limit DNARNA hybridization
20.
“Blunt ends” are
a. produced by DNA probes b. single stranded ends of double stranded DNA that are
created by the cutting of restriction enzymes b. double stranded ends of DNA that are
spliced to the end of some other DNA sample
e. single stranded ends of DNA that are cut
out by probes
21.
Most genetic engineering experiments include four stages. Which of the following is NOT
one of them?
a. cleavage of DNA b. production of recombinant DNA c. cloning d. screening
e. treatment of disease
22.
Cloning is a method of producing a large number of cells all of which
a. are able to manufacture none of their own proteins
b. are capable of cleaving DNA from
any source c. can synthesize human interferon
d. contain the plasmid with the desired
DNA fragment
e. might be able to cause cancer in humans
23.
A commercially significant human protein now produced in bacteria is
a. hemoglobin b. gamma globulin c. AZT
d. human insulin
e. HIV vaccine
24.
___ is the transfer of normal genes into body cells to correct a genetic defect
a. reverse transcriptase b. nucleic acid hybridization
c. PCR
d. gene therapy
25.
A transgenic organism
a. has genes from a different member of its own species b. has genes from a different
species
c. has genes that are not its own
d. has genes that have been altered in some
way