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Title of paper (Times New Roman, Font size: 18, Bold, Center aligned, reddish brown color) Author 1#1, Author 2#2,Author 3#3..(Times New Roman, Font size:12 ,Bold ,Center aligned) #1 Address, Phone no. and email Id of Author 1(Times New Roman, Font size:12,Center aligned) #2 Address, Phone no. and email Id of Author 2(Times New Roman, Font size:12,Center aligned) #3 Address, Phone no. and email Id of Author 3(Times New Roman, Font size:12,Center aligned) ABSTRACT (Times New Roman, Font size:12 ,Bold , left aligned, Reddish brown color) (Times New Roman, Font size:12 ,Justify aligned, paragraph :single spacing)Bicep is the genus in which the well-known Indian medicinal plant Brahmi (Bacopa monnieri) is included. Three of the most common allied species of brammi isBacopa eisenii,Bacopa repens and B.Carolinian. Here an attempt was made to find out the relationship between the four species of bacopa with the help of NADH dehydrogenase subunit F, the common enzyme sequence available in the NCBI databank. We studied physic-chemical parameters like amino acid frequency, isoelectric point, aliphatic index, GRAVY, charges, extinction coefficient, hydrophobicity and antigenicity. The domains in the protein and their secondary structure along with peptide sequences and Trans membrane sequences also predicted. Key words: Bacopa, Brahmi, NADH dehydrogenase subunit F, antigenicity, peptide, transmembrane, phylogeny. Corresponding Author: Ashokan.K.V(Name of corresponding Author) INTRODUCTION (Times New Roman, Font size: 12, Bold, left aligned Reddish brown color) (Times New Roman, Font size:12 ,Justify aligned, paragraph :single spacing)Bacopa is a genus of 70 - 100 aquatic and palatial herbs belonging to the family plantaginaceae, distributed throughout the warmer regions of the world [1]. Table1. Physicochemical properties (Times New Roman, Font size:12 ,left aligned,)- put the table near to its description Information Sequence type Length (Number of amino acids) Accession Number B. eisenii Protein B.repens Protein B.Carolinian Protein 288 265 702682 ABR28437 ABR28436 AF123677_1 B. mornnieri Protein ABP88016 MATERIALS AND METHODS (Times New Roman, Font size:12 ,Bold ,left aligned Reddish brown color) (Times New Roman, Font size:12 ,Justify aligned, paragraph :single spacing) Fig 1: Complexity ployt of NADH (Times New Roman, Font size: 12, center aligned)- put the figure near to its description Continue your points according to paper CONCLUSION (Times New Roman, Font size:12 ,Bold ,left aligned Reddish brown color) (Times New Roman, Font size:12 ,Justify aligned, paragraph :single spacing) REFERENCE (Times New Roman, Font size:12 ,Bold ,left aligned Reddish brown color) (Times New Roman, Font size:12 ,Justify aligned, paragraph :single spacing) [1] C.D.K.Cook, B.J. Gupta, E.M.Rix, J.Scheller, and M.Serrz, Water plants of the world, Jurh, The Hague. Court, A. B. (1957),Sundry notes on three Victori, 1974. [2] A. Agrawal,M.N.Pandey, and G.P.Dubey, Management of mental deficiency by an indigenous drug, Brahmi (Bacopa monniera),Pharmacopsychoecologia., Vol.6(1), pp.15,1993. Page Layout Your paper must use a page size corresponding to A4. The margins must be set as follows: Top = Bottom =Left = Right = 1" Page Numbers, Headers and Footers Do not use Page numbers, headers and footers.