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Photosynthesis - continued - 6CO2 + 12H2O Light, Pigments Enzymes C6H12O6 + 6O2 + 6H2O - Takes place in two major stages: 1. Light dependent stage Requires direct involvement of light to occur. Light is captured by pigments located in the thylakoids which are found inside the chloroplast. Energy from light is used to create ATP and NADPH (nicotinamide adenine dinucleotide phosphate). Both are high energy compounds. A process called the electron transport chain, located in thylakoid membrane, occurs in this stage and is responsible for creating the ATP and NADPH. H20 is used in this stage and O2 (waste) is made. 2. Light independent stage Series of chemical reactions that produce glucose A Rxn B Rxn C Rxn D Rxn E Sometimes is called the “dark” stage but this is misleading. This stage can occur in either dark or light, does not need direct sunlight. Uses the products of the light dependent stage, ATP and NADPH. Involves direct input of CO2 Produces glucose Occurs in a cycle, called the Calvin Cycle, due to the series of chemical reactions starting and ending with the same molecule. (draw picture) All steps are enzyme driven Calvin Cycle takes place in a liquid filled area of the chloroplasts called the stoma It takes 6 “turns” of the cycle to create one glucose The 6 carbons for the glucose come from the 6CO2 and is called carbon-fixation due to carbons being taken from an inorganic compound (CO2) and being “fixed” into an organic compound (C6H12 O6) Occurs in a cycle, called the Calvin Cycle, due to the series of chemical reactions starting and ending with the same molecule. A Rxn Rxn B Rxn Rxn C Rxn Rxn D Rxn Rxn A All steps are enzyme driven Calvin cycle takes place in a liquid filled area of the chloroplast called the stroma It takes six “turns” of the cycle to create one glucose The six carbons for the glucose come from six CO2 and is called carbon-fixation due to carbons being taken from an inorganic compound (CO2) and being “fixed” into an organic compound (C6H12O6). CO2 6 Carbon Sugar ATP RuBP (5 Carbon Sugar) ADP Calvin Cycle NADPH NADP C 6 turns C6H12O6 (glucose) Photosynthesis - continued Chloroplast outer membrane inner membrane granum stroma thylakoid