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Kigali Institute of Science and Technology Institut des Sciences et de Technologie de Kigali Avenue de l'Armée, B.P. 3900 Kigali, Rwanda INSTITUTE EXAMINATIONS – ACADEMIC YEAR 2012 - 2013 SUPPLEMENTARY EXAMINATION FACULTY OF SCIENCE SCIENCE 3: APPLIED BIOLOGY SECOND YEAR SEMESTER II BIO 3222: MICROBIAL DIVERSITY Date: /2013 TIME: 2 HOURs MAXIMUM MARKS = 60 _____________________________________________________________________ INSTRUCTIONS 1. 2. 3. 4. 5. 6. 7. This paper contains TWO sections. Section A is compulsory, and Answer any TWO out of the Three questions in Section B. No written materials are allowed into the Examination Room. Write all your answers in the answer booklet provided. Do not forget to write your Registration Number. Do not write any answers on this question paper. Where appropriate draw large, clear and well labeled diagram in your answers 1 Section A : (Compulsory Questions) Attempt all of the following Short-answer and Definition Questions. (20 marks) 1. Give brief comparative definitions for each of the following paired terms: (2 marks each) a) b) c) d) Classification versus Taxonomy of microorganisms Binomiai Nomenclature versus Identification of microorganisms The Archaea verus the Eucarya group of microbes Yeasts versus molds 2. Define each of the following related terminologies? a) Protista, Eucayota and Monera b) Microbial morphology and microbial physiology c) Mitochondria, chloroplasts and endoplasmic reticulum d) Peptidoglycan layer, Outer membrane and Nuclear membrane (2 marks each) 3. What can you tell about the G+C mole% content of microbial DNAs and what is its significant importance? (2 marks) 4. Which kind of Gram-negative bacteria among the aerobic, facultative anaerobic and obligate anaerobic organisms are associated with the ability of growing autotrophically with H2 their energy source and the ability of using methane gas as a carbon source ? (2.5 marks) 5. Give at least one distinguishing property for each of the following bacterial groups: (1.5 marks each) a) Gonococcus c) Staphylococcus b) Streptococcus d) Salmonella e) Escherichia coli 6. a) What are the three basic criteria that Whittacher used to regroup organisms into five different kingdom levels? (1 marks) b) What do we mean by the term « Opportunistic Pathogens»? Give any two examples of the Gram-negative opportunistic bacterial pathogens of humans. (1 marks) Section B : (Descriptive and Essay-type Questions) (40 marks) Attempt any Two of the following Three questions. 1. a) Explain the morphological distinguishing features the Mycoplasmas compared to the other groups of bacteria. b) In which division and class are these groups classified? c) What kind of effects do these Mycoplasmas have on the human body? (20 marks) 2. a) What are the different general taxonomical features shown by the Molds and Yeasts? b) Indicate one or two commonly known Divisions of these groups of microbes. c) Explain their taxonomical diversity or classification systems giving an example of one genus and species each. (20 marks for each) 2 3. Write an essay on the descriptive features of the morphological, physiological, and taxonomical characteristics which can distinguish between Streptococcus and Staphylococcus bacteruak groups. (20 marks) END OF THE QUESTIONS and GOOD LUCK 3