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Episcopal Academy AP Biology Investigation 8: Transformation C. Gray Mitchell After completing lab & lab quiz 6I on http://www.phschool.com/science/biology_place/labbench/lab6/intro.html answer the following questions: 1. What is genetic transformation? Cite one example of natural transformation and one example of engineered transformation. 2. What organism will we use in our lab and what medium will we grow it on? 3. What ‘transformation factor’ will we use (plasmid, gene gun, virus)? 4. What is ‘log phase’ bacterial growth and why is it so important that we transform only those colonies that are in ‘log phase’? 5. What does it mean to make bacterial cells ‘competent’? What chemical do we use to induce ‘competence’? 6. What is the purpose of ‘heat shock’? 7. How do we ascertain that E. coli cells have been successfully transformed? 8. How is transformation efficiency calculated? 9. Name two ways that modern biotechnology uses transformation.
