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BY www.whatisthebiotechnology.com www.wahtisthebiotechnology.com 1 INTRODUCTION PURPOSE METHODS 1.Vectors mediated transformation 2.Direct gene transfer/ without vector Transformation www.wahtisthebiotechnology.com 2 Plant transformation is the technique or way to integration and expression of desire gene or DNA fragment in plant cell for improvement plant breed is known as plant transformation. Transformation involving integration of introduced gene in the plant nuclear genome www.wahtisthebiotechnology.com 3 The improvement to crop plants and other plants for desire purposes like production of diseases free plants, improve quality of plants, improving quantity (product), salt, drought resistant plants. And other purpose of this technology is used as an experimental tool for plant physiology and to study research work. www.wahtisthebiotechnology.com 4 Vectors mediated transformation-plasmids or viruses used as vectors. Example Cauliflower mosaic virus (CaMV), tobacco mosaic viruses www.wahtisthebiotechnology.com 5 www.wahtisthebiotechnology.com 6 Direct gene transfer/ without vector Transformation Chemicals techniques Physical techniques www.wahtisthebiotechnology.com 7