Survey
* Your assessment is very important for improving the work of artificial intelligence, which forms the content of this project
Download 9/24/2012 Worksheet#7 KEY
Transcript
Molecular genetics Terms to study before coming to next SI. Eventually you will see these in a quiz we will have in SI. I won't give the answers for these; you can Google a reliable source to check yourself DNA helicase DNA topoisomerase DNA ligase DNA polymerase 3 Okazaki fragments Leading strand Lagging strand Origins of replication Histone proteins Nucleosomes Centromere Gene Transcription Translation ____________ injected _________ type R and __________ type S bacteria into a mouse. Griffith, living, heat-killed What happened? Why? The mouse died because the DNA of the S bacteria was incorporated into the R strain, making a new virulent S strain. The strands of DNA are made of deoxyribonucleotides T F The backbone of DNA contains: a. nucleotides b. sugars c. phosphates d. purine and pyrimidine e. b and c What is found at the 5' end and 3' end of the backbone? Phosphate at the 5' end and hydroxyl on the sugar at the 3' end What nucleic bases pair with each other? A pairs with T and C pairs with G Which bases are pyrimidines and which are purines? See the book We write out DNA from ____ to _____ on the upper strand and from _____ to _______ on the complementary strand. 5' – 3', 3' – 5' What types of bonds are found in DNA Phosphodiester bonds, hydrogen bonds, covalent bonds DNA replication is explained with the ______________ model Semi-conservative Mendelson and Stahl got equal fractions of heavy and light DNA after the second replication T F What enzyme makes DNA replication possible? DNA Polymerase What does deoxynucleoside triphosphate do? The anhydride bond breaks leaving a nucleotide and a phosphate on the DNA strand, and a pyrophosphate not in the DNA strand When DNA replicates, which way does the leading strand grow? It grows from 5' – 3', but DNA is antiparallel. So the leading strand grows successively as the DNA unzips. How does the lagging strand grow? DNA primase adds short new strands called primer RNA, Okazaki fragments are made by DNA polymerase, and DNA ligase joins Okazaki fragments. Which is the correct strand match for the DNA sequence 5'CGTAGTCAGT3'? 5'ACTGACTACG3' What is chromatin fiber? Packed nucleosomes of DNA How many protein encoding genes in the human body? 21000 What are the 3 stages of translation? Initiation, Elongation, Termination What are transcription factors? They recruit the RNA polymerase What are the modifications on eukaryotic genes? Introns, Splicing, Tail, Cap What is an exon? Regions that are not spliced How is the RNA different from DNA? It is single strand, It has uracil, It is not replicated What is the promoter? The protein that binds to DNA to start transcription
