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CHAPTER 20 OUTLINE VIRUSES AND BACTERIA THE TRUE BACTERIA: Most ancient of organisms TWO BACTERIAL KINGDOMS: Archaebacteria (ancient) and Eubacteria (true) Prokaryotes (both): lack nuclear membrane, mitochondria, Golgi apparatus, ER, lysosomes Divide by fission SYSTEMATICS: Eubacterial evolution: no consensus…??? See 20.7 diagram: Gram + and Gram - (react with Gram’s stain) ANATOMY: see size chart overhead 20.8 Size Cell Shape: should be review (look at pictures on p. 562) Cell Wall Different from plant cell walls (plant wall is made of cellulose, eubacteria wall is made of murein Murein: polymer of polysaccharides cross linked with amino acids( also called peptidoglycan) INTERESTING: Penicillin inhibits the formation of exposed peptidoglycan, and therefore interferes with reproduction of gram+ bacteria. Mycoplasmas Capsules: Endospores: mostly rod shaped form these resting cells. HOW BACTERIA MOVE: Flagella UNLIKE eucaryotic cell flagella. HOW BACTERIA REPRODUCE: Binary fission: 2 equal daughter cells essentially like the parent cell. ENORMOUS reproductive potential Ie. division once every 20 minutes. This would result in 500,000 “offspring” in 6 hours… There is genetic variation…review chapter 10 a. Conjugation (DNA transferred from donor cells) b. transformation (DNA from dead cells c. transduction (DNA carried by viruses) OBTAINING ENERGY AND RAW MATERIALS: most are heterotrophic (parasites/saprophytes) Many are aerobic, some anaerobic Obligate anaerobes: Facultative anaerobes Some perform both fermentation and the Krebs/Electron Transport Chain, if oxygen is present. There are at least 10 other types of fermentation besides lactic acid and alcoholic. BACTERIAL PHOTOSYNTHESIS: Most are chemoheterotrophs Chemoautotrophs: (in ch.40 we will discuss nitrifying bacteria in more depth) Photosynthetic bacteria: (photoheterotrophs), Example: Green and purple anaerobic photosynthetic bacteria possess chromatophores, vesicles that contain pigments/enzymes for the photosynthesis (only photosystem I) Do NOT possess chlorophyll a Photoautotrophs: Example: Cyanobacteria: aerobic photosynthetic bacteria. -cyanobacteria generate molecular oxygen as a by-product of photosynthesis -contain: phycocyanin (blue pigment). -may contain: phycoerythrin (red pigment). FIXING NITROGEN: cyanobacteria and other anaerobic bacteria Conversion of N2 into ammonia (NH3). LIMITED BY THE ENZYME, NITROGENASE, WHICH IS INACTIVE IN PRESENCE OF OXYGEN…see below… HOW does cyanobacteria do it? They are oxygen-generating photosynthesizers…. Most are filamentous forms that produce heterocysts, where the fixation takes place. DISEASE CAUSING BACTERIA: many…STD, TB, tetanus… Can disease tissues, can interfere with normal functions of organism, can produce toxins Exotoxins are produced by living bacterial cells. BENEFICIAL BACTERIA: far outnumber harmful ones Ie. nitrogen fixing bacteria…vital to agriculture, decomposers, human digestive system, industrial processes, food industry, antibiotic production (read p. 572-573 for ideas) ARCHAEBACTERIA: Differ from eubacteria in a number of ways: a. cell walls, if present have different chemical properties b. lipids in their cell membranes are branched, not straight c. “younger” kingdom than the eubacterial kingdom Four groups: confined to EXTREME habitats 1. Methanogens 2. Halophiles 3. Sulfur Reducers: 4. Thermoacidophilic Bacteria