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Study Station 1: Macromolecules A macromolecule is a very large molecule made up of smaller units called monomers. Macromolecules are formed by dehydration synthesis in which water molecules are removed to form bonds. There are four major macromolecules: carbohydrates, lipids, proteins, and nucleic acids. Carbohydrates are commonly called sugars aka saccharides. When iodine is placed in a carbohydrate solution, the iodine will turn from yellow to black. Lipids include the fatty acids, oils, and waxes. They can be saturated or unsaturated. Proteins are made from chains of amino acids. There are 20 different amino acids. Lastly, DNA and RNA are our nucleic acids. DNA is double stranded while RNA is single stranded. Both carry genetic information of our cells. Practice Question 1: A student filled two Petri dishes with a clear cornstarch gel, then marked the letter “X” invisibly onto the gel in Petri dish 1 with a damp cotton swab. He then placed saliva from his mouth onto a second cotton swab and used that swab to mark the letter “X” invisibly onto the gel in Petri dish 2. Fifteen minutes later, he rinsed both Petri dishes with a dilute solution of iodine to indicate the presence of starch. The surface of Petri dish 1 turned completely blue, indicating starch. Most of the surface of Petri dish 2 was blue, except the letter “X” was clear, as shown above. The most probable explanation of the clear “X” is that A B C D the starch in the gel was absorbed by the damp cotton swab. the iodine reacted with a chemical in the saliva and broke down. a chemical in the saliva broke down the starch in the gel. the saliva prevented the iodine from contacting the starch in the gel. Study Station 2: Cell/Plasma Membrane When you think about a membrane, imagine it is like a big plastic bag with some tiny holes. That bag holds all of the cell pieces and fluids inside the cell and keeps any nasty things outside the cell. The holes are there to let some things move in and out of the cell. Thus, the cell membrane is semi-permeable. The cell membrane is not one solid piece. Everything in life is made of smaller pieces and a membrane is no different. Compounds called proteins and phospholipids make up most of the cell membrane. The phospholipids make the basic bag. The proteins are found around the holes and help move molecules in and out of the cell. Scientists describe the organization of the phospholipids and proteins with the fluid mosaic model. That model shows that the phospholipids are in a shape like a head and a tail. The heads like water (hydrophilic) and the tails do not like water (hydrophobic). The tails bump up against each other and the heads are out facing the watery area surrounding the cell. The two layers of cells are called the bilayer. Practice Question 2a: The cell membrane of the red blood cell will allow water, oxygen, carbon dioxide, and glucose to pass through. Because other substances are blocked from entering, this membrane is called A B C D perforated. semi-permeable. non-conductive. permeable. Practice Question 2b: The plasma membrane of a cell consists of A protein molecules arranged in two layers with polar areas forming the outside of the membrane. B two layers of lipids organized with the nonpolar tails forming the interior of the membrane. C lipid molecules positioned between two carbohydrate layers. D protein molecules with polar and nonpolar tails. Study Station 3: Proteins and Enzymes Proteins are made of monomers called amino acids. There are 20 amino acids that exist. The sequence and different amounts of amino acids makes proteins different from each other. Enzymes are a type of proteins that catalyze (i.e. increase the rates of) chemical reactions. In enzymatic reactions, the molecules at the beginning of the process are called substrates, and the enzyme converts them into different molecules, the products. Enzymes are extremely selective and work like a “lock and key.” Enzymes will work slowly in cold temperatures. They will also denature (deform) in extremely hot temperatures and extreme acidity or basicity. Practice Question 3: Some snake venoms are harmful because they contain enzymes that destroy blood cells or tissues. The damage caused by such a snake bite could best be slowed by A applying ice to the bite area. B drinking large amounts of water. C inducing vomiting. D increasing blood flow to the area Study Station 4: Prokaryotes vs Eukaryotes Prokaryotic cells, like bacteria, have no nuclei, while eukaryotic cells (animals, plants, fungi) do have true nuclei. Despite their apparent differences, these two cell types have a lot in common. They perform most of the same kinds of functions, and in the same ways. Both are enclosed by plasma membranes, filled with cytoplasm, and loaded with small structures called ribosomes. Both have DNA which carries the archived instructions for operating the cell. Despite all of these similarities, the differences are also clear. Eukaryotic cells are much larger and much more complex than prokaryotic cells. If we take a closer look at the comparison of these cells, we see the following differences: 1. Eukaryotic cells have a true nucleus, bound by a double membrane. Prokaryotic cells have no nucleus. 2. Eukaryotic DNA is linear; prokaryotic DNA is circular (it has no ends). 3. The cytoplasm of eukaryotic cells is filled with a large, complex collection of organelles, many of them enclosed in their own membranes; the prokaryotic cell contains no membrane-bound organelles which are independent of the plasma membrane. Practice Question 4: Eukaryotic cells are differentiated from prokaryotic cells because eukaryotic cells A are much smaller. B have permeable membranes. C have a higher rate of reproduction. D have nuclei. Study Station 5: Eukaryotic Organelles Below is a list of organelles that are commonly found in eukaryotic cells, which are found in plant, animal, and fungi cells. Organelle Function Nucleus The “brains” of the cell, the nucleus directs cell activities and contains genetic material called chromosomes made of DNA. Mitochondria Ribosomes Make energy out of food Make protein Golgi Apparatus Make, process and package proteins Lysosome Contains digestive enzymes to help break food down Endoplasmic Reticulum Called the "intracellular highway" because it is for transporting all sorts of items around the cell. Vacuole Used for storage, vacuoles usually contain water or food. (Are you are thirsty? Perhaps your vacuoles need some water!) Plant cells also have: Chloroplasts Use sunlight to create food by photosynthesis Cell Wall For support Practice Question 5: Which cellular organelle is responsible for packaging the proteins that the cell secretes? A cytoskeleton B cell membrane C lysosome D Golgi apparatus Study Station 6: DNA DNA stands for deoxyribonucleic acid and is one of the two types of nucleic acid found in our cells. The name describes what the molecule is. DNA is: A Nucleic Acid (i.e. it is made up of strings of nucleotides bonded together) Has a backbone made of phosphate and deoxyribose DNA is a record of instructions telling the cell what its job is going to be. A good analogy for DNA as a whole is a set of blueprints for the cell, or computer code telling a PC what to do. It is written in a special alphabet that is only four letters long! Unlike a book or computer screen, DNA isn't flat and boring - it is a beautiful curved ladder. We call this shape a double helix. The letters of the DNA alphabet (called bases) make up the rungs, special sugars and other atoms make up the handrail. The rungs are very special. Each one has a name, but they prefer to be called by their initials: A, T, C and G They do not like to be by themselves so always pair up with a friend. But they are very choosy about their friends: A and T are best friends and always hang out together G and C are best friends and always hang out together Practice Question 6: Which of the following base pair sequences could be produced in DNA replication? A 5' AGTCUT 3' 3' TCUGTA 5' B 5' AGTCAT 3' 3' TCAGTA 5' C 5' AGTCAT 3' 3' CTGACG 5' D 5' AGTCAT 3' 3' UCAGUA 5' Study Station 7: Central Dogma Protein Synthesis Steps in Protein Synthesis: STEP 1: The first step in protein synthesis is the transcription of mRNA from a DNA gene in the nucleus. The mRNA migrates from the nucleus into the cytoplasm. The mRNA goes to a ribosome, which is either free floating in the cytoplasm or attached to the rough endoplasmic reticulum. In the cytoplasm, protein synthesis is actually initiated by the AUG codon (3 nucleotides) on mRNA. The tRNA which initiates the protein synthesis has the amino acid methionine attached. The process continues with the next codon. The amino acid chain will attach together to make a polypeptide (a protein). Practice Question 7: 5' ATCAGCGCTGGCGGT 3' The above sequence of DNA is part of a gene. How many amino acids are coded for by this segment? A) 5 B) 8 C) 10 D) 20 Study Station 8: Mitosis Mitosis is the process by which a eukaryotic cell separates the chromosomes in its cell nucleus into two identical sets, in two separate nuclei. It is generally followed immediately by cytokinesis, which divides the nuclei, cytoplasm,organelles and cell membrane into two cells containing roughly equal shares of these cellular components. Mitosis and cytokinesis together define the mitotic (M) phase of the cell cycle— the division of the mother cell into two daughter cells, genetically identical to each other and to their parent cell. There are 4 phases of mitosis: prophase, metaphase, anaphase, and telophase. This accounts for approximately 10% of the cell cycle. In mitosis, the starting cell is diploid; it has 2 sets of DNA (one from the egg and one from the sperm). In the end of this cell cycle, the daughter cells are also diploid. So 2n (2 sets of DNA) 2n (2 sets of DNA). Practice Question 8: Which of the following sequences represents chromosome number during mitosis? A) n + n 2n B) 2n 2n C) n n D) 2n n Study Station 9: Meiosis Meiosis is a special type of cell division necessary for sexual reproduction in eukaryotes. The cells produced by meiosis are gametes or spores. In many organisms, including all animals andland plants (but not some other groups such as fungi), gametes are called sperm and egg cells. The outcome of meiosis is four (genetically unique) haploid cells, compared with the two (genetically identical) diploid cells produced from mitosis. Meiosis begins with one diploid cell containing two copies of each chromosome—one from the organism’s mother and one from its father—and produces four haploid cells containing one copy of each chromosome. Each of the resulting chromosomes in the gamete cells is a unique mixture of maternal and paternal DNA, resulting in offspring that are genetically distinct from either parent. This gives rise to genetic diversity in sexually reproducing populations. Practice Question 9: Which of the following statements correctly describes meiosis? A) Cells divide only once during meiosis. B) Meiosis does not occur in reproductive cells. C) The cells produced at the end of meiosis are genetically identical to the parent cell. D) The cells produced at the end of meiosis contain half the number of chromosomes as the parent cell. Study Station 10: Genetics DNA is wrapped together to form structures called chromosomes. Most cells in the human body have 23 pairs of chromosomes, making a total of 46. Individual sperm and egg cells, however, have just 23 unpaired chromosomes. You received half of your chromosomes from your mother's egg and the other half from your father's sperm cell. A male child receives an X chromosome from his mother and a Y chromosome from his father; females get an X chromosome from each parent. Genes are sections or segments of DNA that are carried on the chromosomes and determine specific human characteristics, such as height or hair color. Because you have a pair of each chromosome, you have two copies of every gene (except for some of the genes on the X and Y chromosomes in boys, because boys have only one of each). Heredity is the passing of genes from one generation to the next. You inherit your parents' genes. Heredity helps to make you the person you are today: short or tall, with black hair or blond, with brown eyes or blue. Genes can be either dominant or recessive. Dominant genes show their effect even if there is just one mutation in one copy of that gene pair; the one mutation "dominates" the normal back-up copy of the gene, and the characteristic shows itself. A person can be born with gene mutations, or they can happen over a lifetime. Mutations can occur when cells are aging or have been exposed to certain chemicals or radiation. Fortunately, cells usually recognize these types of mutations and repair them by themselves. Other times, however, they can cause illnesses, such as some types of cancer. If the gene mutation exists in egg or sperm cells, children can inherit the gene mutation from their parents. When the mutation is in every cell of the body (meaning a child was born with it), the body is not able to "repair" the gene change. Study Station 11: Punnett Squares One of the easiest ways to calculate the mathematical probability of inheriting a specific trait was invented by an early 20th century English geneticist named Reginald Punnett. His technique employs what we now call a Punnett square. This is a simple graphical way of discovering all of the potential combinations of genotypes that can occur in children, given the genotypes of their parents. It also shows us the odds of each of the offspring genotypes occurring. Setting up and using a Punnett square is quite simple once you understand how it works. You begin by drawing a grid of perpendicular lines: Next, you put the genotype of one parent across the top and that of the other parent down the left side. For example, if parent pea plant genotypes were YY and yy respectively, the setup would be: y Note that only one letter goes in each box for the parents. It does not matter which parent is on the side or the top of the Punnett square. Next, all you have to do is fill in the boxes by copying the row and column-head letters across or down into the empty squares. This gives us the predicted frequency of all of the potential genotypes among the offspring each time reproduction occurs. Remember, homozygous means you have the same alleles from each parent (AA or aa). Heterozygous means you received different alleles from your parent (Aa). y Biology Review for Final: Stations Directions: 1. You have eight (8) minutes at each station before rotating. 2. Read the paragraph of information at the station. 3. Summarize the information on your own paper following the instructions below. 4. Answer the practice question for that station. 5. Rotate to the next station and perform steps “a-d” again. For Each Station: a) Write the station name down b) Summarize the important points of the information provided. Use bullet points. c) What is the answer to the multiple choice question? d) What is the evidence that proves your answer is right? (It is okay to quote a sentence from the information) Biology Review for Final: Stations Directions: 1. You have eight (8) minutes at each station before rotating. 2. Read the paragraph of information at the station. 3. Summarize the information on your own paper following the instructions below. 4. Answer the practice question for that station. 5. Rotate to the next station and perform steps “a-d” again. For Each Station: a) Write the station name down b) Summarize the important points of the information provided. Use bullet points. c) What is the answer to the multiple choice question? d) What is the evidence that proves your answer is right? (It is okay to quote a sentence from the information)