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Chapter 9 Genetics of Bacteria Study Outline 1. Overview of genetic transfer in bacteria 2. Bacterial conjugation a. Bacteria can transfer genetic material during conjugation b. Conjugation requires direct physical contact c. An F+ strain transfers an F factor to an F- strain during conjugation d. Bacteria may contain different types of plasmids 3. Conjugation and mapping via HFR strains a. Hfr strains contain an F factor integrated into the bacterial chromosome b. Hfr strains can transfer a portion of the bacterial chromosome to recipient cells c. Conjugation experiments can map genes along the E. coli chromosome d. A genetic map of the E. coli chromosome has been obtained from many conjugation studies 4. Bacterial transduction a. Bacteriophages transfer genetic material from one bacterial cell to another vi transduction b. Cotransduction can be used to map genes that are within 2 minutes of each other 5. Bacterial transformation 6. Horizontal gene transfer