An efficient acetylation of dextran using in situ activated acetic
... purified by re-precipitation and their purity was verified by spectroscopic techniques. It was found that on increasing the molar ratio of acetic anhydride to dextran, the degree of substitution (DS) also increased, which means that in this way, the DS can be controlled. This observation is obvious ...
... purified by re-precipitation and their purity was verified by spectroscopic techniques. It was found that on increasing the molar ratio of acetic anhydride to dextran, the degree of substitution (DS) also increased, which means that in this way, the DS can be controlled. This observation is obvious ...
MBOATReviewBST - Imperial Spiral
... Ghrelin was identified by Kojima et al [18] during a search for a binding partner of an orphan G protein coupled receptor (GHS-R1a) which stimulates secretion of growth hormones in the pituitary gland. After cleavage of pro-ghrelin (117 amino acids), ghrelin (28 amino acids) undergoes post-translati ...
... Ghrelin was identified by Kojima et al [18] during a search for a binding partner of an orphan G protein coupled receptor (GHS-R1a) which stimulates secretion of growth hormones in the pituitary gland. After cleavage of pro-ghrelin (117 amino acids), ghrelin (28 amino acids) undergoes post-translati ...
CRISPR/Cas9 as a tool for creation of p53 knock
... (strain U87, details may be found in Golding et al. 2009)6 with mutated/defective p53 (strain U87-281G, which can be generated from U87 by expressing mutant p53, or mp53) were more sensitive to radiotherapy than glioma cells with wild-type (wt) p53 (U87), after one of the genes that plays a role in ...
... (strain U87, details may be found in Golding et al. 2009)6 with mutated/defective p53 (strain U87-281G, which can be generated from U87 by expressing mutant p53, or mp53) were more sensitive to radiotherapy than glioma cells with wild-type (wt) p53 (U87), after one of the genes that plays a role in ...
Lab 6
... which the acyl group is the acetyl group. Such reactions are called acetylation reactions. These reactions involve the transfer of an acetyl acyl group to an oxygen atom (i.e., the substitution of an acetyl group for an H atom bonded to oxygen). When alcohols or phenols are acylated, the products ar ...
... which the acyl group is the acetyl group. Such reactions are called acetylation reactions. These reactions involve the transfer of an acetyl acyl group to an oxygen atom (i.e., the substitution of an acetyl group for an H atom bonded to oxygen). When alcohols or phenols are acylated, the products ar ...
Ribosomes: Cashing in on crystals
... density between the bound and free ribosome crystals. The overall architecture of the 70S ribosome seen here is similar to that observed previously by cryo-electron microscopy. Furthermore, structural features seen in the 30S and 50S electron density maps are also observed, such as the long rRNA hel ...
... density between the bound and free ribosome crystals. The overall architecture of the 70S ribosome seen here is similar to that observed previously by cryo-electron microscopy. Furthermore, structural features seen in the 30S and 50S electron density maps are also observed, such as the long rRNA hel ...
Np73 is capable of inducing apoptosis by co
... Key words: Np73, apoptosis, BH3-only proteins, Fanconi anaemia, p53, specificity protein 1 (Sp1). Cite this article as: Bioscience Reports (2015) 35, e00198, doi:10.1042/BSR20150039 ...
... Key words: Np73, apoptosis, BH3-only proteins, Fanconi anaemia, p53, specificity protein 1 (Sp1). Cite this article as: Bioscience Reports (2015) 35, e00198, doi:10.1042/BSR20150039 ...
Plant organelle proteomics
... were apparently free of contaminating membranes from other organelles, although some of the proteins have been found in proteomic studies of thylakoids, stroma and plastid envelopes. It is unclear whether plastoglobules are cross contaminants of those suborganelle preparations, or whether proteins a ...
... were apparently free of contaminating membranes from other organelles, although some of the proteins have been found in proteomic studies of thylakoids, stroma and plastid envelopes. It is unclear whether plastoglobules are cross contaminants of those suborganelle preparations, or whether proteins a ...
Sec14p-like proteins regulate phosphoinositide homoeostasis and
... Sec9p t-SNARE (target membrane soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptor) function Stt4p and Mss4p reside in the yeast plasma membrane [22]. This raises the possibility that SFH proteins modulate exocytic and/or endocytic events at the plasma membrane. To investig ...
... Sec9p t-SNARE (target membrane soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptor) function Stt4p and Mss4p reside in the yeast plasma membrane [22]. This raises the possibility that SFH proteins modulate exocytic and/or endocytic events at the plasma membrane. To investig ...
Sequence ID: ref|WP_006700522.1
... ChIP-chip or ChIP-seq to detect chromatin modifications: 17 mods are associated with active genes in CD-4 T cells ...
... ChIP-chip or ChIP-seq to detect chromatin modifications: 17 mods are associated with active genes in CD-4 T cells ...
Chem*3560 Lecture 11: Regulation by proteolytic cleavage
... The reaction that activates trypsinogen is induced by the protease enteropeptidase, which is a membrane protein exposed on the external surface of the mucosal cells that line the duodenum. Enteropeptidase is specific for the sequence Lys-Ile found in trypsinogen. The quantity of enteropeptidase pres ...
... The reaction that activates trypsinogen is induced by the protease enteropeptidase, which is a membrane protein exposed on the external surface of the mucosal cells that line the duodenum. Enteropeptidase is specific for the sequence Lys-Ile found in trypsinogen. The quantity of enteropeptidase pres ...
Minus end - Website Staff UI
... A. Electron micrograph of a myosin II thick filaments B. Schematic diagram of myosin molecules which are aggregated by means of their tail regions with their head projecting to the outside of the filaments C. A small section of a myosin II filaments as reconstructed from electron micrograph ...
... A. Electron micrograph of a myosin II thick filaments B. Schematic diagram of myosin molecules which are aggregated by means of their tail regions with their head projecting to the outside of the filaments C. A small section of a myosin II filaments as reconstructed from electron micrograph ...
Functions of Ribosome-Associated Chaperones and their Interaction
... Initially, TF was discovered as a cytosolic protein involved in the translocation of the outer membrane protein pro-OmpA across the plasma membrane as it has the ability to promote the insertion of chemically denatured pro-OmpA into membrane vesicles [31]. However, TF is not essential for viability ...
... Initially, TF was discovered as a cytosolic protein involved in the translocation of the outer membrane protein pro-OmpA across the plasma membrane as it has the ability to promote the insertion of chemically denatured pro-OmpA into membrane vesicles [31]. However, TF is not essential for viability ...
NUTRICELL START [en tête: NUTRIENTS]
... Diammonium phosphate 55%, inactivated yeast (S. cerevisiae), organic nitrogen < 10% DM (expressed as N), yeast autolysates (S. cerevisiae), organic nitrogen < 12% DM (expressed as N), amino acids 10% to 20% DM (DNFB method), yeast cell envelopes (S. cerevisiae) 6%, thiamine 0.1%. Non-allergenic, GMO ...
... Diammonium phosphate 55%, inactivated yeast (S. cerevisiae), organic nitrogen < 10% DM (expressed as N), yeast autolysates (S. cerevisiae), organic nitrogen < 12% DM (expressed as N), amino acids 10% to 20% DM (DNFB method), yeast cell envelopes (S. cerevisiae) 6%, thiamine 0.1%. Non-allergenic, GMO ...
Tubulin Subunit Carboxyl Termini Determine Polymerization Efficiency
... if conservative substitutions are counted) suggesting a com- PAG (FMC).The lowergel buffer pH was9.2, and SDS in the mon evolutionary origin (3). Both subunits are acidic proteins, electrode buffer (0.1%) was lauryl sulfate (Sigma) or equivalent (14). but the fl subunit is slightly more acidic than ...
... if conservative substitutions are counted) suggesting a com- PAG (FMC).The lowergel buffer pH was9.2, and SDS in the mon evolutionary origin (3). Both subunits are acidic proteins, electrode buffer (0.1%) was lauryl sulfate (Sigma) or equivalent (14). but the fl subunit is slightly more acidic than ...
pdf format - Faculty members Homepages
... phosphorylation (1). Evidence suggests that these covalent modifications play important roles in several biological activities involving chromatin, e.g., transcription and replication (2). Histone deacetylases (HDACs) catalyze the removal of the acetyl group from the lysine residues in the N-termina ...
... phosphorylation (1). Evidence suggests that these covalent modifications play important roles in several biological activities involving chromatin, e.g., transcription and replication (2). Histone deacetylases (HDACs) catalyze the removal of the acetyl group from the lysine residues in the N-termina ...
the PDF file - Sanjaxx Soccer School
... Protein intake has become quite a popular topic these days amongst athletes, who enjoy swinging back protein shakes on the regular. Athletes have a greater need for protein intake for tissue repair and it can be used as fuel for endurance athletes. Soccer athletes need 0.5 – 0.8 gram of protein for ...
... Protein intake has become quite a popular topic these days amongst athletes, who enjoy swinging back protein shakes on the regular. Athletes have a greater need for protein intake for tissue repair and it can be used as fuel for endurance athletes. Soccer athletes need 0.5 – 0.8 gram of protein for ...
AMINO ACIDS AND THEIR POLYMERS
... ________ 12. The order in which the amino acids of a peptide molecule are linked is called the amino acid sequence. ________ 13. Proteins have a characteristic three-dimensional shape that is determined by the interactions between the amino acids in the protein. ...
... ________ 12. The order in which the amino acids of a peptide molecule are linked is called the amino acid sequence. ________ 13. Proteins have a characteristic three-dimensional shape that is determined by the interactions between the amino acids in the protein. ...
Document
... Monosaccharide and disaccharide are osmotically active substances, contributing to the solute potential of cell sap. Cellulose is structural materials to build up cell wall. Starch and glycogen are storage materials. ...
... Monosaccharide and disaccharide are osmotically active substances, contributing to the solute potential of cell sap. Cellulose is structural materials to build up cell wall. Starch and glycogen are storage materials. ...
Human/Mouse/Rat PP2A Catalytic Subunit Antibody
... BACKGROUND Protein Phosphatase 2A (PP2A) dephosphorylates serine and threonine residues in proteins. This ubiquitously expressed enzyme plays a critical role in modulating cell survival, growth factor responses, and neurotransmission. Phosphorylation near the Cterminus at Y307 of the catalytic su ...
... BACKGROUND Protein Phosphatase 2A (PP2A) dephosphorylates serine and threonine residues in proteins. This ubiquitously expressed enzyme plays a critical role in modulating cell survival, growth factor responses, and neurotransmission. Phosphorylation near the Cterminus at Y307 of the catalytic su ...
Lecture 31
... To control proteolysis- most proteases are made as precursor proteins (proprotcins or zymogens) this form is inactive or has very low activity To get to the active form, need a protease or can have slow auto-proteolysis, it depends upon the system. 2T method in cukaryotes, almost every protease has ...
... To control proteolysis- most proteases are made as precursor proteins (proprotcins or zymogens) this form is inactive or has very low activity To get to the active form, need a protease or can have slow auto-proteolysis, it depends upon the system. 2T method in cukaryotes, almost every protease has ...
Probing chromatin-modifying enzymes with chemical tools Wolfgang
... the sulfonium-methyl group of SAM is replaced by an aziridine, and generates bisubstrate analogue 5 that serves as inhibitor of PRMT1.21 While the reaction is useful for generating PMT inhibitors, it does not allow characterization of novel PMT substrates.22 To identify the substrates of PMTs, surro ...
... the sulfonium-methyl group of SAM is replaced by an aziridine, and generates bisubstrate analogue 5 that serves as inhibitor of PRMT1.21 While the reaction is useful for generating PMT inhibitors, it does not allow characterization of novel PMT substrates.22 To identify the substrates of PMTs, surro ...
Intelligent Systems and Molecular Biology-short-version-ics
... Constraints – design of large synthetic genes DNA nanotechnology and space-filling DNA tetrahedra ...
... Constraints – design of large synthetic genes DNA nanotechnology and space-filling DNA tetrahedra ...
Acetylation
Acetylation (or in IUPAC nomenclature ethanoylation) describes a reaction that introduces an acetyl functional group into a chemical compound. (Deacetylation is the removal of the acetyl group.)Acetylation refers to the process of introducing an acetyl group (resulting in an acetoxy group) into a compound, namely the substitution of an acetyl group for an active hydrogen atom. A reaction involving the replacement of the hydrogen atom of a hydroxyl group with an acetyl group (CH3 CO) yields a specific ester, the acetate. Acetic anhydride is commonly used as an acetylating agent reacting with free hydroxyl groups. For example, it is used in the synthesis of aspirin and heroin.