T. Takahashi, B. C. Vo Ngo, L. Xiao, G. Arya, and M. J. Heller
... mimics, most have failed and only a few have produced minimal reaction rates that can barely be considered catalytic. One particular approach we have focused on is the use of short-sequence peptides that contain key catalytic groups in close proximity. In this study, we designed six different peptid ...
... mimics, most have failed and only a few have produced minimal reaction rates that can barely be considered catalytic. One particular approach we have focused on is the use of short-sequence peptides that contain key catalytic groups in close proximity. In this study, we designed six different peptid ...
the function and synthesis of ribosomes
... binding close to the pivot point of the head and sterically blocking its movement28. Antibiotic-resistant mutations in r-protein S5 might reduce the stability of the head–body interaction, so that mobility is maintained even in the presence of the antibiotic. Paromomycin binds to a loop that is invo ...
... binding close to the pivot point of the head and sterically blocking its movement28. Antibiotic-resistant mutations in r-protein S5 might reduce the stability of the head–body interaction, so that mobility is maintained even in the presence of the antibiotic. Paromomycin binds to a loop that is invo ...
Refining the Definition of Plant Mitochondrial
... Met aminopeptidase cleavage is known to depend on the length of the side chain of the second amino acid (Sherman et al., 1985; Giglione and Meinnel, 2001). If the second incorporated amino acid is Ala (A), Gly (G), Pro (P), Ser (S), or Thr (T), the initiator Met is systemically removed. If the secon ...
... Met aminopeptidase cleavage is known to depend on the length of the side chain of the second amino acid (Sherman et al., 1985; Giglione and Meinnel, 2001). If the second incorporated amino acid is Ala (A), Gly (G), Pro (P), Ser (S), or Thr (T), the initiator Met is systemically removed. If the secon ...
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... The design of enzymes with improved or non-native properties has become a common approach [40–42]. Engineering TGases may provide solutions to increase their applicability in biocatalytic contexts. TG2 has been engineered towards catalyzing amide bond formation between various synthetic substrates, ...
... The design of enzymes with improved or non-native properties has become a common approach [40–42]. Engineering TGases may provide solutions to increase their applicability in biocatalytic contexts. TG2 has been engineered towards catalyzing amide bond formation between various synthetic substrates, ...
Package `PPInfer`
... Title Inferring functionally related proteins using protein interaction networks Description Interactions between proteins occur in many, if not most, biological processes. Most proteins perform their functions in networks associated with other proteins and other biomolecules. This fact has motivate ...
... Title Inferring functionally related proteins using protein interaction networks Description Interactions between proteins occur in many, if not most, biological processes. Most proteins perform their functions in networks associated with other proteins and other biomolecules. This fact has motivate ...
Hitting the Target: Emerging Technologies in the Search for Kinase
... searches for candidate Akt substrates with Scansite (16). This approach successfully identified known and novel Akt substrates that were confirmed by using conventional methods of substrate validation. One problem with this method is that in any given mass range Scansite will predict several strong ...
... searches for candidate Akt substrates with Scansite (16). This approach successfully identified known and novel Akt substrates that were confirmed by using conventional methods of substrate validation. One problem with this method is that in any given mass range Scansite will predict several strong ...
english,
... enantioselection, since both alcohols-enantiomers were glucosylated, with different amount ratios. The obtained diastereomeric b-glucosides of racemic alcohols were not separated by TLC with the solvent applied. The racemic mixture of (±)-2-phenyl-propan-1-ol produced through transglucosylation two ...
... enantioselection, since both alcohols-enantiomers were glucosylated, with different amount ratios. The obtained diastereomeric b-glucosides of racemic alcohols were not separated by TLC with the solvent applied. The racemic mixture of (±)-2-phenyl-propan-1-ol produced through transglucosylation two ...
Histones - scientia.global
... Histones form the bulk of the protein component of chromatin – a complex of macromolecules found in cells, consisting of DNA, RNA and protein. Originally, histones were thought to be only involved in packing chromosomal DNA in eukaryotic cells, but now, they are clearly also important players in reg ...
... Histones form the bulk of the protein component of chromatin – a complex of macromolecules found in cells, consisting of DNA, RNA and protein. Originally, histones were thought to be only involved in packing chromosomal DNA in eukaryotic cells, but now, they are clearly also important players in reg ...
Synthetic biology for engineering acetyl coenzyme a
... enzyme complex, and this was found to be higher than the endogenous mitochondrial PDH complex of yeast. As discussed in the paper, PDH expression has been claimed in an earlier study and here a PDC deletion strain was used as background strain (16). In this study, the authors expressed the PDH subun ...
... enzyme complex, and this was found to be higher than the endogenous mitochondrial PDH complex of yeast. As discussed in the paper, PDH expression has been claimed in an earlier study and here a PDC deletion strain was used as background strain (16). In this study, the authors expressed the PDH subun ...
TFE3 contains two activation domains, one acidic and the other
... is contained within the 102 amino acid C-terminal portion of TFE3 shown to be sufficient for activation (Fig. 1 A). Interestingly, although the proline content in the highly conserved region is low (3/38), all three prolines are perfectly conserved. The two TFE3 activation domains activate transcrip ...
... is contained within the 102 amino acid C-terminal portion of TFE3 shown to be sufficient for activation (Fig. 1 A). Interestingly, although the proline content in the highly conserved region is low (3/38), all three prolines are perfectly conserved. The two TFE3 activation domains activate transcrip ...
Properties of a newly characterized protein of the bovine - K-REx
... Reaction of affinity-purified mouse anti-protein X and mouse anti-transacetylase antibodies with the subunits and polypeptides prepared from the kidney or heart pyruvate dehydrogenase complex and with the kidney a-ketoglutarate dehydrogenase complex ...
... Reaction of affinity-purified mouse anti-protein X and mouse anti-transacetylase antibodies with the subunits and polypeptides prepared from the kidney or heart pyruvate dehydrogenase complex and with the kidney a-ketoglutarate dehydrogenase complex ...
characterization of proteins from the cytoskeleton of giardia lamblia
... dimers in organelle- or site-specific patterns. Giardia is an endoparasitic flagellate with a number of microtubular organelles. Extraction with Triton isolates from this organism the eight flagellar axonemes and a large disc cytoskeleton that normally supports the cell's adhesive sucker. Although t ...
... dimers in organelle- or site-specific patterns. Giardia is an endoparasitic flagellate with a number of microtubular organelles. Extraction with Triton isolates from this organism the eight flagellar axonemes and a large disc cytoskeleton that normally supports the cell's adhesive sucker. Although t ...
Characterization of Low Molecular Weight Glutenin Subunits by
... Wheat grain of the cultivar Yecora Rojo, lot CWC-141, was obtained from the California Wheat Commission. IE-purified glutenin was prepared from the endosperm fraction. Flour was milled in a Brabender Quadrumat Senior mill (C. W. Brabender, South Hackensack, NJ), and flour protein was 11.0% on a 13% ...
... Wheat grain of the cultivar Yecora Rojo, lot CWC-141, was obtained from the California Wheat Commission. IE-purified glutenin was prepared from the endosperm fraction. Flour was milled in a Brabender Quadrumat Senior mill (C. W. Brabender, South Hackensack, NJ), and flour protein was 11.0% on a 13% ...
Microsoft Word
... The amine 37 and 3--carbolinic acid (29) were coupled using DCC/DMAP in dry DCM to give corresponding amide 38 in 70% yield. Also in a different experiment amine 37 and 3-carbolinic acid (29) were coupled using HOBT/EDCI to give amide 38 in 68% yield. Acetinide protected amide compound (38) was re ...
... The amine 37 and 3--carbolinic acid (29) were coupled using DCC/DMAP in dry DCM to give corresponding amide 38 in 70% yield. Also in a different experiment amine 37 and 3-carbolinic acid (29) were coupled using HOBT/EDCI to give amide 38 in 68% yield. Acetinide protected amide compound (38) was re ...
Identification of the tRNA-binding Protein Arc1p as a Novel Target of
... both arc1⌬ and arc1⌬los1⌬ mutants, indicating that biotinylation of Arc1p is not essential for activity. ...
... both arc1⌬ and arc1⌬los1⌬ mutants, indicating that biotinylation of Arc1p is not essential for activity. ...
intact-protein based sample preparation strategies for
... National Cancer Institute (Emmert-Buck et al., 1996). It has been successfully used to isolate single cells within a tissue section (Emmert-Buck et al., 1996). Cells can be selected according to their phenotypic and functional characteristics. The major limitation of the LCM approach for proteomics ...
... National Cancer Institute (Emmert-Buck et al., 1996). It has been successfully used to isolate single cells within a tissue section (Emmert-Buck et al., 1996). Cells can be selected according to their phenotypic and functional characteristics. The major limitation of the LCM approach for proteomics ...
Amino acids
... Importance of Proteins 1- Proteins form the building units of all body cells 2-Almost all enzymes are protein in nature 3- Many hormones are protein in nature as insulin and thyroxine 4- Certain biological compounds necessary for maintenance of life as hemoglobin, myoglobin etc are protein substanc ...
... Importance of Proteins 1- Proteins form the building units of all body cells 2-Almost all enzymes are protein in nature 3- Many hormones are protein in nature as insulin and thyroxine 4- Certain biological compounds necessary for maintenance of life as hemoglobin, myoglobin etc are protein substanc ...
Amino acids
... Importance of Proteins 1- Proteins form the building units of all body cells 2-Almost all enzymes are protein in nature 3- Many hormones are protein in nature as insulin and thyroxine 4- Certain biological compounds necessary for maintenance of life as hemoglobin, myoglobin etc are protein substanc ...
... Importance of Proteins 1- Proteins form the building units of all body cells 2-Almost all enzymes are protein in nature 3- Many hormones are protein in nature as insulin and thyroxine 4- Certain biological compounds necessary for maintenance of life as hemoglobin, myoglobin etc are protein substanc ...
TARGET: a new method for predicting protein subcellular
... localization of the entire proteome (Kumar et al., 2002; Huh et al., 2003); however, such diligent feats are not practicable in all species. Therefore, experimental annotation of protein subcellular localization is not able to keep up with the large number of sequences that continue to emerge from t ...
... localization of the entire proteome (Kumar et al., 2002; Huh et al., 2003); however, such diligent feats are not practicable in all species. Therefore, experimental annotation of protein subcellular localization is not able to keep up with the large number of sequences that continue to emerge from t ...
Topological studies suggest that the pathway of the protons through
... been conserved. In yeast subunit 6, two amino acid substitutions leading to oligomycin resistance have been identified [20]. The mutated residues would correspond to positions 195 and 256 in subunit a of E. coli, located in the middle of the hydrophobic segments 5 and 7. These residues might be dire ...
... been conserved. In yeast subunit 6, two amino acid substitutions leading to oligomycin resistance have been identified [20]. The mutated residues would correspond to positions 195 and 256 in subunit a of E. coli, located in the middle of the hydrophobic segments 5 and 7. These residues might be dire ...
Identification of cellular proteins that bind to the human
... MRC Retrovirus Research Laboratory, Department of Veterinary Pathology, University of Glasgow, Bearsden Road, Glasgow G61 1QH, U.K. ...
... MRC Retrovirus Research Laboratory, Department of Veterinary Pathology, University of Glasgow, Bearsden Road, Glasgow G61 1QH, U.K. ...
Short transmembrane domains with high
... Fig. 1. Geometric features of TMDs in metazoan type II Golgi and plasma membrane proteins. (A) Average volume of amino acids of Type II Golgi and plasma membrane protein TMDs. Values are mean 6 2 s.e.m. calculated in a window of five amino acids at each position in the TMD for all proteins in the da ...
... Fig. 1. Geometric features of TMDs in metazoan type II Golgi and plasma membrane proteins. (A) Average volume of amino acids of Type II Golgi and plasma membrane protein TMDs. Values are mean 6 2 s.e.m. calculated in a window of five amino acids at each position in the TMD for all proteins in the da ...
Chapter 14 Preparing Semisynthetic and Fully Synthetic Histones
... 1. Introduction Nucleosomes, the fundamental unit of chromatin, are protein–DNA complexes that package and organize DNA in the nucleus of the cell (1). The core nucleosome particle contains two copies each of four histone proteins: H3, H4, H2A, and H2B. Each of these histone proteins has multiple si ...
... 1. Introduction Nucleosomes, the fundamental unit of chromatin, are protein–DNA complexes that package and organize DNA in the nucleus of the cell (1). The core nucleosome particle contains two copies each of four histone proteins: H3, H4, H2A, and H2B. Each of these histone proteins has multiple si ...
國立清華大學 - 罕見疾病基金會
... Human NADH dehydrogenase (ubiquinone) Fe-S protein 7 (NDUFS7), one of the most conserved core subunits of mitochondrial complex I, plays an important role in the oxidative phosphorylation system (OXPHOS). This protein binds one iron-sulfur cluster N2 (tetranuclear) which is the terminal redox center ...
... Human NADH dehydrogenase (ubiquinone) Fe-S protein 7 (NDUFS7), one of the most conserved core subunits of mitochondrial complex I, plays an important role in the oxidative phosphorylation system (OXPHOS). This protein binds one iron-sulfur cluster N2 (tetranuclear) which is the terminal redox center ...
Function and specificity of 14-3-3 proteins in the regulation of
... 1993; Toroser and Huber, 1997). However, there is a sitespeci®c regulatory interaction between 14-3-3 proteins and Ser-229 of spinach SPS which inhibits SPS activity (Toroser et al., 1998). The interaction of 14-3-3s and target proteins can be reduced in vitro by 5¢-AMP which interacts with 14-3-3s ...
... 1993; Toroser and Huber, 1997). However, there is a sitespeci®c regulatory interaction between 14-3-3 proteins and Ser-229 of spinach SPS which inhibits SPS activity (Toroser et al., 1998). The interaction of 14-3-3s and target proteins can be reduced in vitro by 5¢-AMP which interacts with 14-3-3s ...
Acetylation
Acetylation (or in IUPAC nomenclature ethanoylation) describes a reaction that introduces an acetyl functional group into a chemical compound. (Deacetylation is the removal of the acetyl group.)Acetylation refers to the process of introducing an acetyl group (resulting in an acetoxy group) into a compound, namely the substitution of an acetyl group for an active hydrogen atom. A reaction involving the replacement of the hydrogen atom of a hydroxyl group with an acetyl group (CH3 CO) yields a specific ester, the acetate. Acetic anhydride is commonly used as an acetylating agent reacting with free hydroxyl groups. For example, it is used in the synthesis of aspirin and heroin.