Overexpression of DNA repair genes is associated with metastasis
... cycle regulation, DNA replication, DNA repair) and many biological processes have already been characterized as directly related to metastasis [6–8]. Among these genes, we have particularly focused on those involved in the maintenance of genetic stability in human cells in order to determine whether ...
... cycle regulation, DNA replication, DNA repair) and many biological processes have already been characterized as directly related to metastasis [6–8]. Among these genes, we have particularly focused on those involved in the maintenance of genetic stability in human cells in order to determine whether ...
Knox. The Gene Genie.
... Scientists in both labs realized that Cas9 might be useful for genome editing, a type of genetic engineering that uses enzymes as molecular pruning shears. The enzymes, called nucleases, create breaks at specific sites in the double-stranded DNA helix; a cell then repairs the break, sometimes incorp ...
... Scientists in both labs realized that Cas9 might be useful for genome editing, a type of genetic engineering that uses enzymes as molecular pruning shears. The enzymes, called nucleases, create breaks at specific sites in the double-stranded DNA helix; a cell then repairs the break, sometimes incorp ...
Slide 1
... The Harlow laboratory at Harvard Medical School would like to automate their plasmid DNA purification process to increase throughput, improve purity, and reduce cost. Our focus was to design and demonstrate the feasibility of an improved single unit operation which meets the purity and yield require ...
... The Harlow laboratory at Harvard Medical School would like to automate their plasmid DNA purification process to increase throughput, improve purity, and reduce cost. Our focus was to design and demonstrate the feasibility of an improved single unit operation which meets the purity and yield require ...
Cytogenetic and molecular cytogenetic analysis in clinical genetics
... DNA probes specific to the telomeres of all human chromosomes. Useful for the detection of chromosome structural abnormalities such as cryptic translocations or small deletions that are not easily visualized by standard ...
... DNA probes specific to the telomeres of all human chromosomes. Useful for the detection of chromosome structural abnormalities such as cryptic translocations or small deletions that are not easily visualized by standard ...
Single-stranded DNA-binding Proteins
... copies (E. coli and human mitochondrial SSB) of the OB fold in its active state. The SSBs are responsible for several important functions in DNA replication, including: 1. configuring the ssDNA for use by DNA polymerase – this entails arraying the template DNA in an extended single-stranded conformat ...
... copies (E. coli and human mitochondrial SSB) of the OB fold in its active state. The SSBs are responsible for several important functions in DNA replication, including: 1. configuring the ssDNA for use by DNA polymerase – this entails arraying the template DNA in an extended single-stranded conformat ...
Pursuing DNA Catalysts for Protein Modification
... CONSPECTUS: Catalysis is a fundamental chemical concept, and many kinds of catalysts have considerable practical value. Developing entirely new catalysts is an exciting challenge. Rational design and screening have provided many new small-molecule catalysts, and directed evolution has been used to o ...
... CONSPECTUS: Catalysis is a fundamental chemical concept, and many kinds of catalysts have considerable practical value. Developing entirely new catalysts is an exciting challenge. Rational design and screening have provided many new small-molecule catalysts, and directed evolution has been used to o ...
A multistep epigenetic switch enables the stable
... involve a DNA conformational change. Total cell fluorescence (log10 a.u.) Total cell fluorescence (log10 a.u.) DNase I footprinting studies have not identified an OxyR-binding site in the proximal The origin of the partial expression state was investigated by region13; therefore, it is unlikely that ...
... involve a DNA conformational change. Total cell fluorescence (log10 a.u.) Total cell fluorescence (log10 a.u.) DNase I footprinting studies have not identified an OxyR-binding site in the proximal The origin of the partial expression state was investigated by region13; therefore, it is unlikely that ...
Gateway LR Clonase Enzyme Mix
... owned by Invitrogen Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). No license is conveyed un ...
... owned by Invitrogen Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). No license is conveyed un ...
Non-Enzymatic, Low Temperature Fluorescence in situ
... couples), alternative mechanisms for ISH may exist. For example, small amounts of single-stranded target DNA present either naturally, or as the result of the fixation process alone, might in some cases be sufficient to allow visualization of probetarget binding sites using low temperature protocols ...
... couples), alternative mechanisms for ISH may exist. For example, small amounts of single-stranded target DNA present either naturally, or as the result of the fixation process alone, might in some cases be sufficient to allow visualization of probetarget binding sites using low temperature protocols ...
... the abscisic acid response element (ABRE) and drought response element (DRE) (Table 1). A number of cis elements have been defined based on in silico and functional analysis data on a large number of promoters and is available in the database. The tools like PLACE help in identifying these sequences ...
Biochemical Defects Associated with Cancer
... MP1 cellular extract contains all MMR factors that are required for repair except MutLα A titration was done to identify conditions under which these factors aren’t limiting for repair Repair efficiency CT loop ...
... MP1 cellular extract contains all MMR factors that are required for repair except MutLα A titration was done to identify conditions under which these factors aren’t limiting for repair Repair efficiency CT loop ...
![P[acman]: A BAC Transgenic Platform for Targeted Insertion of](http://s1.studyres.com/store/data/015771109_1-230af42a78d308ddb0e06d89019d2ef2-300x300.png)
P[acman]: A BAC Transgenic Platform for Targeted Insertion of
... docking sites were identified within the Drosophila genome, potentially bypassing desired integration events in engineered attP sites. Fortunately, they did not seem receptive to attB plasmids, because all integration events were at the desired attP sites (11). Thus, recovery of large DNA fragments ...
... docking sites were identified within the Drosophila genome, potentially bypassing desired integration events in engineered attP sites. Fortunately, they did not seem receptive to attB plasmids, because all integration events were at the desired attP sites (11). Thus, recovery of large DNA fragments ...
letters Solution structure of the DNA-binding domain of MafG
... (149°) is considerably larger than the typical value for the common HTH motif (∼110°)10. Thus, MafG(1–76) may have a protein–DNA interaction mode distinct from that of the ordinary HTH-type DNA-binding domains. Recently, the 3D structure of the DNA-binding domain of Skn-1, a developmental transcript ...
... (149°) is considerably larger than the typical value for the common HTH motif (∼110°)10. Thus, MafG(1–76) may have a protein–DNA interaction mode distinct from that of the ordinary HTH-type DNA-binding domains. Recently, the 3D structure of the DNA-binding domain of Skn-1, a developmental transcript ...
Text Book of Molecular Biology
... then link together the two ends,the twisting formed deformation is locked into the system. This deformation is shown as supercoiling, since it manifests itself as a coiling of the DNA axis around itself in a higher-order coil, and corresponds to a change in linking number from the relaxed circular ...
... then link together the two ends,the twisting formed deformation is locked into the system. This deformation is shown as supercoiling, since it manifests itself as a coiling of the DNA axis around itself in a higher-order coil, and corresponds to a change in linking number from the relaxed circular ...
Chromosome Project
... Chromosome Project You will be assigned a chromosome number to research. Answer the following questions in a Powerpoint or Prezi. The presentation must be emailed to me at [email protected] on the due date with your name as the subject line. Please do not print the presentation. Use the following we ...
... Chromosome Project You will be assigned a chromosome number to research. Answer the following questions in a Powerpoint or Prezi. The presentation must be emailed to me at [email protected] on the due date with your name as the subject line. Please do not print the presentation. Use the following we ...
lec03-1
... Common subunits: RPB5, 6, 8, 10 and 12 are the common subunits. Nonessential subunits: two of the subunits appear to be essential only under certain conditions: RPB4 and RPB9. Deletion mutants of these two function well at normal temperatures, but fail to grow at either higher or lower temperatures ...
... Common subunits: RPB5, 6, 8, 10 and 12 are the common subunits. Nonessential subunits: two of the subunits appear to be essential only under certain conditions: RPB4 and RPB9. Deletion mutants of these two function well at normal temperatures, but fail to grow at either higher or lower temperatures ...
Roles of the Amino Group of Purine Bases in the Thermodynamic
... a conformation that did not alter the overall B-form of the DNA helix. These spectra were also insensitive to the salt concentration and solvent composition investigated in this study (Figure S1). The CD spectral data suggested that the differences in thermodynamic stabilities among the duplexes res ...
... a conformation that did not alter the overall B-form of the DNA helix. These spectra were also insensitive to the salt concentration and solvent composition investigated in this study (Figure S1). The CD spectral data suggested that the differences in thermodynamic stabilities among the duplexes res ...
Hybridization of labeled DNA
... of unknown significance where we could find no published link or only a weak link to neuropsychiatric disorders including 6p22.3, 7q11.22, 7q31.32, 9p24.1, 12p11.22, 13q34 (2 non-overlapping), 17p12, 17p13.2, 17p13.3, 19q13.42, CHST, EFEMP1, KCNJ12, and SCLT1, although a few overlapping events have ...
... of unknown significance where we could find no published link or only a weak link to neuropsychiatric disorders including 6p22.3, 7q11.22, 7q31.32, 9p24.1, 12p11.22, 13q34 (2 non-overlapping), 17p12, 17p13.2, 17p13.3, 19q13.42, CHST, EFEMP1, KCNJ12, and SCLT1, although a few overlapping events have ...
typing methods - Micro-Rao
... using agarose gel electrophoresis. The pattern stained by ethidium bromide and examined under UV light. Different strains of the same species have different REA profiles because of variations in their DNA sequences. Advantages: All the strains can by typed with good reproducibility. Disadvantages: T ...
... using agarose gel electrophoresis. The pattern stained by ethidium bromide and examined under UV light. Different strains of the same species have different REA profiles because of variations in their DNA sequences. Advantages: All the strains can by typed with good reproducibility. Disadvantages: T ...
2016‐12‐15 1
... What is a DNA molecule? A piece of double stranded DNA (i.e. a double helix of DNA) What is the difference between a chromosome and a chromatid? A replicated chromosome is made up of two chromatids which are joined by the centromere. The chromatids separate from each other during mitosis and is d ...
... What is a DNA molecule? A piece of double stranded DNA (i.e. a double helix of DNA) What is the difference between a chromosome and a chromatid? A replicated chromosome is made up of two chromatids which are joined by the centromere. The chromatids separate from each other during mitosis and is d ...
ATTORNEY FOR APPELLANT John H. Watson Public Defender
... statistically reliable result by comparing more loci among the huge number comprising a strand of one human’s DNA with that of another. Id. at 340-41. The results from the STR analysis in this case indicated that the chance that a white male other than Troxell was the source of the hair found in the ...
... statistically reliable result by comparing more loci among the huge number comprising a strand of one human’s DNA with that of another. Id. at 340-41. The results from the STR analysis in this case indicated that the chance that a white male other than Troxell was the source of the hair found in the ...
Unit A: Global Agriculture
... 1. What makes up DNA? (backbone and the rungs of ladder) 2. Who discovered DNA? (Two male scientists) 3. DNA is the instruction for the building blocks of what? (insulin is an example of this) 4. What genetic disease did Patrice suffer from? 5. What “project” was completed to map a human’s DNA? 6. W ...
... 1. What makes up DNA? (backbone and the rungs of ladder) 2. Who discovered DNA? (Two male scientists) 3. DNA is the instruction for the building blocks of what? (insulin is an example of this) 4. What genetic disease did Patrice suffer from? 5. What “project” was completed to map a human’s DNA? 6. W ...
Transposons - iPlant Pods
... • Produces stress-inducible networks (cold, salt, others?) • Generates dominant alleles Naito et al, Nature, 2009 ...
... • Produces stress-inducible networks (cold, salt, others?) • Generates dominant alleles Naito et al, Nature, 2009 ...
DNA supercoil
DNA supercoiling refers to the over- or under-winding of a DNA strand, and is an expression of the strain on that strand. Supercoiling is important in a number of biological processes, such as compacting DNA. Additionally, certain enzymes such as topoisomerases are able to change DNA topology to facilitate functions such as DNA replication or transcription. Mathematical expressions are used to describe supercoiling by comparing different coiled states to relaxed B-form DNA.As a general rule, the DNA of most organisms is negatively supercoiled.