11357_2014_9648_MOESM1_ESM
... Supplemental figure 3S To validate Infinium 450K BeadChip data methylation 3 CpG sites of TNFa and of DUSP22 were analysed by Pyrosequencing. In addition, two CpG sites in the promoter of the FABP4 gene that are not present on the 450K array were analysed. The obtained results show that the aging ef ...
... Supplemental figure 3S To validate Infinium 450K BeadChip data methylation 3 CpG sites of TNFa and of DUSP22 were analysed by Pyrosequencing. In addition, two CpG sites in the promoter of the FABP4 gene that are not present on the 450K array were analysed. The obtained results show that the aging ef ...
D-loop - BioMed Central
... and a negative AT-skew (-0.2531), i.e. the transcript of the major strand is relatively rich in nucleotides G and T, and correspondently poor in C and A (nucleotide frequencies as follows: T 0.447, C 0.110, A 0.267, G 0.176). This pattern, referred to as the reverse strand bias [1], is also found in ...
... and a negative AT-skew (-0.2531), i.e. the transcript of the major strand is relatively rich in nucleotides G and T, and correspondently poor in C and A (nucleotide frequencies as follows: T 0.447, C 0.110, A 0.267, G 0.176). This pattern, referred to as the reverse strand bias [1], is also found in ...
Bewildering Bs: an impression of the 1st B-Chromosome
... of each of these processes. In summary, Bs may be absent from a certain population because it is beyond the limit of the species' ecological tolerance for B chromosomes and/or because Bs have not reached this locality from their centre of origin. Another intriguing point that was raised is why are t ...
... of each of these processes. In summary, Bs may be absent from a certain population because it is beyond the limit of the species' ecological tolerance for B chromosomes and/or because Bs have not reached this locality from their centre of origin. Another intriguing point that was raised is why are t ...
Final Exam Review Part B - Hudson City School District
... • A. from both somatic and sex cells • B. from only sex cells • C. from only somatic cells • ANSWER: B ...
... • A. from both somatic and sex cells • B. from only sex cells • C. from only somatic cells • ANSWER: B ...
Lesson title: Nucleic acids Lesson date: 30.12.2013 One sentence
... differences between DNA and RNA. 3. Students will be able to infer that the sequence of the nucleic acids in DNA is the key to how DNA provides instructions to the cell. 4. Students will be able to relate this nucleic acids puzzle activity to Watson and Crick’s original discovery of the structure of ...
... differences between DNA and RNA. 3. Students will be able to infer that the sequence of the nucleic acids in DNA is the key to how DNA provides instructions to the cell. 4. Students will be able to relate this nucleic acids puzzle activity to Watson and Crick’s original discovery of the structure of ...
1 - life.illinois.edu
... the K system. When lambda is grown on the K host, the DNA is K modified but not P1 modified. Thus when these phages infects the K strain, they make plaques efficiently because the DNA is not restricted. However, the P1 restriction system degrades the DNA and the frequency of plaques decreases by 1,0 ...
... the K system. When lambda is grown on the K host, the DNA is K modified but not P1 modified. Thus when these phages infects the K strain, they make plaques efficiently because the DNA is not restricted. However, the P1 restriction system degrades the DNA and the frequency of plaques decreases by 1,0 ...
Chapter 14 Notes - Gonzaga High School
... combinations produce four distinct phenotypes in a 9:3:3:1 ratio. This was consistent with Mendel’s results. Mendel repeated the dihybrid cross experiment for other pairs of characters and always observed a 9:3:3:1 phenotypic ration in the F2 generation. Each character appeared to be inherited indep ...
... combinations produce four distinct phenotypes in a 9:3:3:1 ratio. This was consistent with Mendel’s results. Mendel repeated the dihybrid cross experiment for other pairs of characters and always observed a 9:3:3:1 phenotypic ration in the F2 generation. Each character appeared to be inherited indep ...
RiboMAX(TM) Large Scale RNA Production Systems
... This protocol was developed by combining and modifying two published protocols that use HEPES buffer (1) and yeast inorganic pyrophosphatase (2). The development of this system, a comparison to a standard transcription protocol (8), and data demonstrating the enhanced “translatability” of RNA genera ...
... This protocol was developed by combining and modifying two published protocols that use HEPES buffer (1) and yeast inorganic pyrophosphatase (2). The development of this system, a comparison to a standard transcription protocol (8), and data demonstrating the enhanced “translatability” of RNA genera ...
... A C-G basepair and a U-G pair have been provided to help illustrate your answer (i.e. draw some hydrogen bonds!) You need not use both in you answer. Choice A: Formation of double stranded DNA. Choice B: Binding of charged tRNA to mRNA (including wobble basepairing) Choice C: Recognition of specific ...
Nanosep® Centrifugal Devices - Protocols for Use
... Ultrafiltration (UF) is a membrane separation technique used to separate extremely small particles and dissolved molecules in fluids. The primary basis for separation is molecular size, although other factors such as molecule shape and charge can also play a role. Molecules larger than the membrane ...
... Ultrafiltration (UF) is a membrane separation technique used to separate extremely small particles and dissolved molecules in fluids. The primary basis for separation is molecular size, although other factors such as molecule shape and charge can also play a role. Molecules larger than the membrane ...
Am. J. Physiol. 1989, 257, L47
... detect a transcript (in Northern blots). Alternatively, DNA from other species can be analyzed (Zoo blots) (35). This latter strategy is based on the hypothesis that exon sequences are more likely to have been conserved throughout evolution than those of introns. A positive signal detected in a Zoo ...
... detect a transcript (in Northern blots). Alternatively, DNA from other species can be analyzed (Zoo blots) (35). This latter strategy is based on the hypothesis that exon sequences are more likely to have been conserved throughout evolution than those of introns. A positive signal detected in a Zoo ...
Physiological characterization of natural transformation in
... 1986). As more and more details of natural transformation in a number of these bacteria have been elucidated, it is becoming clear that distinct differences exist in this process between these organisms. Wellknown examples are the sequence specificity of DNA uptake in Haemophilus (Goodgal, 1982) and ...
... 1986). As more and more details of natural transformation in a number of these bacteria have been elucidated, it is becoming clear that distinct differences exist in this process between these organisms. Wellknown examples are the sequence specificity of DNA uptake in Haemophilus (Goodgal, 1982) and ...
Plasmids
... (Note: bacterial gene names are not capitalized. Plus sign indicates wild type gene.) ...
... (Note: bacterial gene names are not capitalized. Plus sign indicates wild type gene.) ...
Giant chromosomes
... • The paired chromosomes of oocytes in meiosis consist of numerous chromatin loops arranged along an axis . Chiasma formation is visible at various locations. • Each segment of a lampbrush chromosome consists of a series of chromatin loops, originating from an axis and a condensed structure, the chr ...
... • The paired chromosomes of oocytes in meiosis consist of numerous chromatin loops arranged along an axis . Chiasma formation is visible at various locations. • Each segment of a lampbrush chromosome consists of a series of chromatin loops, originating from an axis and a condensed structure, the chr ...
Gene Section FANCC (Fanconi anaemia complementation group C) Atlas of Genetics and Cytogenetics
... FANCF, and FANCG; this complex is only found in the nucleus. FANCA and FANCG form a complex in the cytoplasm, through a N-term FANCA (involving the nuclear localization signal) - FANCG interaction; FANCC join the complex; phosphorylation of FANCA would induce its translocation into the nucleus.This ...
... FANCF, and FANCG; this complex is only found in the nucleus. FANCA and FANCG form a complex in the cytoplasm, through a N-term FANCA (involving the nuclear localization signal) - FANCG interaction; FANCC join the complex; phosphorylation of FANCA would induce its translocation into the nucleus.This ...
chromosome disorders.
... rearrangements and those that appear balanced cytogenetically but are really unbalanced at the molecular level. • Even when structural rearrangements are truly balanced, they can pose a threat to the subsequent generation because carriers are likely to produce a high frequency of unbalanced gametes ...
... rearrangements and those that appear balanced cytogenetically but are really unbalanced at the molecular level. • Even when structural rearrangements are truly balanced, they can pose a threat to the subsequent generation because carriers are likely to produce a high frequency of unbalanced gametes ...
Construction and genetic characterization of temperature-sensitive mutant alleles of the yeast actin gene.
... The Integrative Replacement/Disruption Strategy. Knowing that the actin gene, when disrupted, results in a recessive lethal mutation presented both opportunities and problems for the design of a scheme to isolate point mutations affecting actin. The major opportunity was the expectation that mutatio ...
... The Integrative Replacement/Disruption Strategy. Knowing that the actin gene, when disrupted, results in a recessive lethal mutation presented both opportunities and problems for the design of a scheme to isolate point mutations affecting actin. The major opportunity was the expectation that mutatio ...
Identification of a novel streptococcal gene cassette mediating
... pharmacological approaches. In Vibrio cholerae, the ciprofloxacin-induced SOS response increased the expression of genes necessary for transfer of the integrating conjugative element SXT (3). SXT carries several antibiotic resistance genes, and the transfer of this element to other strains of V. cho ...
... pharmacological approaches. In Vibrio cholerae, the ciprofloxacin-induced SOS response increased the expression of genes necessary for transfer of the integrating conjugative element SXT (3). SXT carries several antibiotic resistance genes, and the transfer of this element to other strains of V. cho ...
Microsynth GmbH
... a different running behavior, leading to double peaks that are not congruently superposed, but slightly shifted from each other. Sometimes the second primer binding site of the target sequence differs in a few internal positions. In this case, the primer does not bind with the same efficiency as it ...
... a different running behavior, leading to double peaks that are not congruently superposed, but slightly shifted from each other. Sometimes the second primer binding site of the target sequence differs in a few internal positions. In this case, the primer does not bind with the same efficiency as it ...
Trouble Shooting Guide
... a different running behavior, leading to double peaks that are not congruently superposed, but slightly shifted from each other. Sometimes the second primer binding site of the target sequence differs in a few internal positions. In this case, the primer does not bind with the same efficiency as it ...
... a different running behavior, leading to double peaks that are not congruently superposed, but slightly shifted from each other. Sometimes the second primer binding site of the target sequence differs in a few internal positions. In this case, the primer does not bind with the same efficiency as it ...
Restriction Enzymes in Microbiology, Biotechnology and
... R-M system. This grows efficiently in the absence of an R-M system (middle row, left; eop=1) because no restriction enzyme is present, and also in the presence of PstI (middle row, middle; eop=1) because the phage DNA carries the protective, PstIspecific, modification. It grows poorly, however, on t ...
... R-M system. This grows efficiently in the absence of an R-M system (middle row, left; eop=1) because no restriction enzyme is present, and also in the presence of PstI (middle row, middle; eop=1) because the phage DNA carries the protective, PstIspecific, modification. It grows poorly, however, on t ...
Chapter 2 - Institut Montefiore
... It contains vital information that gets passed on to each successive generation. It coordinates the making of itself as well as other molecules (proteins). If it is changed slightly, serious consequences may result. If it is destroyed beyond repair, the cell dies. Changes in the DNA of cells in ...
... It contains vital information that gets passed on to each successive generation. It coordinates the making of itself as well as other molecules (proteins). If it is changed slightly, serious consequences may result. If it is destroyed beyond repair, the cell dies. Changes in the DNA of cells in ...
DNA supercoil
DNA supercoiling refers to the over- or under-winding of a DNA strand, and is an expression of the strain on that strand. Supercoiling is important in a number of biological processes, such as compacting DNA. Additionally, certain enzymes such as topoisomerases are able to change DNA topology to facilitate functions such as DNA replication or transcription. Mathematical expressions are used to describe supercoiling by comparing different coiled states to relaxed B-form DNA.As a general rule, the DNA of most organisms is negatively supercoiled.