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Shotgun DNA sequencing using cloned DNase I
Shotgun DNA sequencing using cloned DNase I

... can be processed. Because the clones are selected randomly, however, the average amount of new sequence information per clone is substantially diminished as the sequence nears completion. ...
Teacher Guide DNA to Protein.ver8 - RI
Teacher Guide DNA to Protein.ver8 - RI

...  Highlight that three nucleotides encode one amino acid in the chain. Also, review that there are available amino acids (which attach to tRNAs) in the cell.  Both transcription and translation can be reviewed step-by-step.  Transfer RNAs (tRNAs) are missing from the model; there is a link at the ...
Activity 1: How Mendel`s Pea Plants Helped Us With Genetics You
Activity 1: How Mendel`s Pea Plants Helped Us With Genetics You

... Genetics is the science of genes and heredity. Inside the nucleus of all your body cells are 23 pairs of chromosomes. Each chromosome carries hundreds to thousands of genes on it. Each gene describes some of your traits. Traits include characteristics like your hair color, eye color, allergies, body ...
Lecture notes: Genetics a.p.
Lecture notes: Genetics a.p.

... They are initially transcribed, but not translated, because they are excised from the transcript before mature RNA leaves the nucleus. Exons: Coding sequences of a gene that are transcribed and expressed RNA splicing: RNA processing that removes introns and joins exons from eukaryotic premRNA; produ ...
x2-5 genetics Sp12
x2-5 genetics Sp12

... Most traits are determined by a combination of genes & environment Even sex can be altered by environment! (ex. exposure to certain hormones/chemicals) BPA is a chemical used in making some plastics. Mimics estrogen and might alter reproductive anatomy and function in early development. ...
ProteinSynthesis
ProteinSynthesis

... • DNA stays in the nucleus, but molecules are built in the cytoplasm of the cell. • So, the code must be copied and moved out into the cytoplasm, where proteins are assembled. • This process is called PROTEIN ...
Chapter07_Outline
Chapter07_Outline

... • E. coli phage  is a temperate phage capable of both lytic and lysogenic, cycles • The DNA of  is a linear molecule with cohesive ends (cos) that pairing yields a circular molecule • In lysogen prophage  is linearly inserted between the gal and bio genes in the bacterial DNA • The sites of  int ...
A conserved repetitive DNA element located in the centromeres of
A conserved repetitive DNA element located in the centromeres of

... proper segregation of chromosomes during mitosis and meiosis, thus ensuring equal distribution of genetic information to the next generation. The centromeric region of higher eukaryotic chromosomes is structurally specified by the primary constriction at which the sister chromatids associate and a p ...
Portfolio 2 - Biology2Nash
Portfolio 2 - Biology2Nash

... three basic parts: a base, a deoxyribose molecule, and a phosphate group. There are four different bases: adenine, cytosine, guanine, and thymine. Only one base is found in each nucleotide. Follow the directions. 1. In the diagram below, the sequence of nucleotides has the code AGCT. Color the diagr ...
Test Blueprint
Test Blueprint

... of molecules, disposal of wastes, function of cellular parts, and synthesis of new molecules (TEKS 4B) The student will compare the structures and functions of different types of biomolecules such as carbohydrates, lipids, proteins, and nucleic acids (TEKS 9A) The student will compare the energy flo ...
Introduction 1
Introduction 1

... Lk = Tw + Wr s = Wr/Tw Lk = linking number: Number of times one edge of ribbon linked around other – topological property  cannot change w/o cutting. (calculate by Lk = Tw+Wr) Twist = winding of Watson around Crick – integrated angle of twist/2p along length, not an integer, necessarily (calculate ...
D2 - Interchim
D2 - Interchim

... Genomic DNA Isolation Kit provides all the necessary reagents and protocols for quickly extracting high-molecular-weight DNA from whole blood, cultured cells, tissue, and bacteria. This kit precludes the need for phenol, chloroform or other organic extraction. RNA is removed by trea™ent with DNase-f ...
Crystal structure of Cas9 in complex with guide RNA and target DNA
Crystal structure of Cas9 in complex with guide RNA and target DNA

Griffith`s Experiment
Griffith`s Experiment

Simulation of Gene Splicing (Genetic Engineering
Simulation of Gene Splicing (Genetic Engineering

... What sticky ends have you made on the human DNA containing the growth hormone gene? What sticky ends have you made on the bacterial DNA (plasmid)? Compare the two. What do you observe? Once the recombinant DNA you just constructed was in existence, the next step would be to insert it into a new bact ...
Using Total Internal Reflection Fluorescence Microscopy, DNA
Using Total Internal Reflection Fluorescence Microscopy, DNA

... example, the pioneering efforts of several research groups have advanced microscopy to the point where it is now possible to visualize individual biological molecules as they perform their functions in real time. These newly emerging technologies are already beginning to revolutionize the ways that ...
QIAquick® Gel Extraction Kit
QIAquick® Gel Extraction Kit

... column stand for 1 min, and then centrifuge for 1 min. After the addition of Buffer EB to the QIAquick membrane, increasing the incubation time to up to 4 min can increase the yield of purified DNA. 10. If purified DNA is to be analyzed on a gel, add 1 volume of Loading Dye to 5 volumes of purified ...
Brooker Chapter 11
Brooker Chapter 11

... • Cap structure is recognized by cap-binding proteins Role of Cap-binding proteins – Movement of some RNAs into the cytoplasm – Early stages of translation – Splicing of introns ...
Viruses Nonliving Structure Reproduction
Viruses Nonliving Structure Reproduction

... Typically, a virus can enter a cell when either a part of the capsid or the spikes in the envelope match receptors in the host cell. Some viruses have a narrow host range and can infect only one or a few species. For example, the smallpox virus infects only humans. Viruses with a ...
overview - El Paso High School
overview - El Paso High School

... DNA to determine its structure. Franklin’s X-ray crystallography convinced them the molecule was helical. Modeling also showed that DNA strands are anti-parallel. (VIDEO 9.1 Deoxyribonucleic acid: A three-dimensional model) FIGURE 9.5 DNA IS a Double Helix Watson and Crick suggested that: • Nucleoti ...
TIANamp Genomic DNA Kit
TIANamp Genomic DNA Kit

chapter 15 chromosomal basis of inheritance
chapter 15 chromosomal basis of inheritance

... XO – Turner syndrome, females of shorter stature and sterile without replacement hormones. 1 in every 5000 live births. Cri du chat – “Cry of the cat,” caused by deletion in chromosome 5. Mental retardation, small head, and a cry that sounds like a distressed cat’s mewing. Usu. die in infancy or ear ...
Molecular genetics of gene expression
Molecular genetics of gene expression

... • What are the differences between DNA and RNA? • Describe the main parts of a gene and their functions. • What role do cis-regulatory elements and trans-acting factors play in gene regulation? • What is responsible for the wide diversity of protein structure found in nature? • In what different way ...
THIRD WORLD NETWORK - Biosafety Information Centre
THIRD WORLD NETWORK - Biosafety Information Centre

... appears doubtful that WHO would have approved of the experiments if it had been formally asked to. Under WHO rules, only specific types of research that are essential for public health may be conducted with smallpox and its genes. These rules appear to preclude Sandia's research, especially since Sa ...
Genetic Recombination in Eukaryotes
Genetic Recombination in Eukaryotes

... Chiasmata at meiosis. ...
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DNA supercoil



DNA supercoiling refers to the over- or under-winding of a DNA strand, and is an expression of the strain on that strand. Supercoiling is important in a number of biological processes, such as compacting DNA. Additionally, certain enzymes such as topoisomerases are able to change DNA topology to facilitate functions such as DNA replication or transcription. Mathematical expressions are used to describe supercoiling by comparing different coiled states to relaxed B-form DNA.As a general rule, the DNA of most organisms is negatively supercoiled.
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