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File - Mrs. LeCompte
File - Mrs. LeCompte

... Therefore, RNA grows one nucleotide at a time in the 5’  3’ direction ...
Biology I ECA Review Standard 7 Genetics
Biology I ECA Review Standard 7 Genetics

... allelic and polygenic traits and illustrate their inheritance patterns over multiple generations.  7.3 Determine the likelihood of the appearance of a specific trait in an offspring given the genetic make-up of the parents.  7.4 Explain the process by which a cell copies its DNA and identify facto ...
L.R. Huang, J.O. Tegenfeldt, J.J. Kraeft, J.C. Sturm, R.H. Austin, E.C. Cox, "Generation of large-area tunable uniform electric fields in microfluid arrays for rapid DNA separation," Tech. Dig. Int. Elect. Dev. Mtg., pp. 363-366 (2001).
L.R. Huang, J.O. Tegenfeldt, J.J. Kraeft, J.C. Sturm, R.H. Austin, E.C. Cox, "Generation of large-area tunable uniform electric fields in microfluid arrays for rapid DNA separation," Tech. Dig. Int. Elect. Dev. Mtg., pp. 363-366 (2001).

... microposts (Fig. 4), fabricated on fused silica. The size of the microposts is comparable to that of a DNA molecule in the ~100kbp range as a random coil. Under DC fields, DNA molecules do not interact with the microposts, and migrate at a constant mobility, independent of their molecular weight [4] ...
Promoters
Promoters

... is twofold • 1. Base-pairing of something to the transcript to destabilize the RNA-DNA hybrid • 2. Something that causes transcription to pause • A normal intrinsic terminator satisfies the first condition by causing a hairpin to form in the transcript, and the second by causing a string of U’s to b ...
testis formation. gene(s) - Journal of Medical Genetics
testis formation. gene(s) - Journal of Medical Genetics

... reasons. (1) It has been shown that H-Y antigen is not required for testis determination.404' (2) ZFX has been shown to escape inactivation,42 so that if two copies of ZFX result in sex reversal, Klinefelter patients should develop as females. In the present case, polyarteritis nodosa (autoimmune in ...
C - MCC Year 12 Biology
C - MCC Year 12 Biology

... Introns are spliced out by spliceosomes leaving only the sequences that will be expressed. This is an example of RNA processing. The introns usually are degraded. The result is a mature mRNA strand that will leave the nucleus to be translated. ...
Document
Document

... •Only 50 μl of food transferred otherwise InstaGene is overwhelmed (~ 5 mg of original material) •Boiling releases DNA from food into the InstaGene solution ...
chapter 25 tortora
chapter 25 tortora

... and speed up a reaction by • acting as a template for substrate orientation, • stressing the substrates and stabilizing the transition state, • providing a favorable microenvironment, • participating directly in the catalytic reaction. ...
Notions of Biochemistry and Molecular Biology Manipulating DNA
Notions of Biochemistry and Molecular Biology Manipulating DNA

... inheritance Proposed the three laws of inheritance Could not explain what genes are or where they physically ...
Concepts of Genetics
Concepts of Genetics

... Avery and his colleagues recognized the genetic and biochemical implications of their work. They observed that “nucleic acids of this type must be regarded not merely as structurally important but as functionally active in determining the biochemical activities and specific characteristics of pneumo ...
Unit 1 - Calderglen High School
Unit 1 - Calderglen High School

... The two strands of the DNA run in opposite directions – i.e. they are antiparallel with deoxyribose and phosphate at 3’ and 5’ ends of each strand. (One strand runs in a 5’ to 3’ direction; the other runs in a 3’ to 5’ direction.) Chromosomes consist of DNA that is tightly coiled around proteins. Th ...
Section 7.1 DNA Cloning with Plasmid Vectors
Section 7.1 DNA Cloning with Plasmid Vectors

... The essence of cell chemistry is to isolate a particular cellular component and then analyze its chemical structure and activity. In the case of DNA, this is feasible for relatively short molecules such as the genomes of small viruses. But genomes of even the simplest cells are much too large to dir ...
Barbara McClintock
Barbara McClintock

... In high school Barbara discovered science and the joy of problem solving. She decided to pursue science in college. Each of the McClintock children’s individuality had been supported and encouraged — at least until adolescence. Then Barbara’s mother became concerned that her children fit into adult ...
Reactive Oxygen Species
Reactive Oxygen Species

... (a) results in the formation of the substrate complex (b). The ferric P450 then accepts the first electron from CPR (cytochrome P450 reductase), thereby being reduced to the ferrous intermediate (c). This intermediate then binds an oxygen molecule to form oxycomplex (d), which is further reduced to ...
Recent progress on the Ada response for inducible repair of DNA
Recent progress on the Ada response for inducible repair of DNA

... adaptive response in other microorganisms. Furthermore, certain algae and fungi growing in saline environments generate MeCl as a product of chloride detoxification (Sedgwick and Vaughan, 1991). MeCl is probably the most abundant methylating agent in our environment (Crutzen and Andreae, 1990). Chem ...
High Frequency of Recombination (Hfr)
High Frequency of Recombination (Hfr)

... ...bacteria exhibiting a high frequency of recombination, – an alteration DNA sequence such that the genotype of subsequent individuals differs from the parent, ...
Worksheet - DNA Code
Worksheet - DNA Code

High Frequency of Recombination (Hfr)
High Frequency of Recombination (Hfr)

... ...bacteria exhibiting a high frequency of recombination, – an alteration DNA sequence such that the genotype of subsequent individuals differs from the parent, ...
GHSGT Ecology/Genetics Review (EcoGenReview)
GHSGT Ecology/Genetics Review (EcoGenReview)

... 13. How does the production of sperm and egg cells differ? A. Each meiotic division produces four sperm cells and one egg cell. Sperm are motile while the egg can not move on its own. B. The egg cell is much larger than a sperm cell. C. The number of chromosomes found in a human sperm is different f ...
Activity #3a - Center for Occupational Research and Development
Activity #3a - Center for Occupational Research and Development

Glossary of Scientific Terms Used in this
Glossary of Scientific Terms Used in this

... samples for their safe transport. These cards can inactivate viruses and bacteria, and still preserve the integrity of the organism’s nucleic acids, which can later be used for molecular diagnostic procedures. Gene: The basic unit of heredity; a sequence of DNA nucleotides on a chromosome. ...
Lec 19
Lec 19

... following example. If a stock preparation of phage is allowed to grow upon E. coli strain C and this stock is then tried upon E. coli C and E. coli K, the titres observed on these two strains will differ by several orders of magnitude, the titre on E. coli K being the lowest. The phages are said to ...
A History of Genetics and Genomics
A History of Genetics and Genomics

... Therefore, his groundbreaking research went largely unnoticed. It was not until 1900 that others, who had performed similar experiments to his, arrived at the same conclusions. Their publications cited his work, leading to a rediscovery of the Mendelian principles. Quickly following the rediscovery, ...
regulation of cell cycle
regulation of cell cycle

... between the start and stop signals code for the various AAs of the gene product but do not include any of the 3 stop codons. When examining an unknown DNA sequence, one indication that it may be part of a gene is the presence of an open reading frame or ORF. An ORF is any stretch of DNA that when tr ...
REPORTING CATEGORY 1: CELL STRUCTURE AND
REPORTING CATEGORY 1: CELL STRUCTURE AND

... 15. Sometimes, mutations in the DNA nucleotide bases can occur. This can be due to environmental factors like UV rays and radiation poisoning or it can be due to mistakes that occur when your cells go through DNA Replication during the S Phase of Interphase. There are two types of Gene Mutations (wh ...
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DNA supercoil



DNA supercoiling refers to the over- or under-winding of a DNA strand, and is an expression of the strain on that strand. Supercoiling is important in a number of biological processes, such as compacting DNA. Additionally, certain enzymes such as topoisomerases are able to change DNA topology to facilitate functions such as DNA replication or transcription. Mathematical expressions are used to describe supercoiling by comparing different coiled states to relaxed B-form DNA.As a general rule, the DNA of most organisms is negatively supercoiled.
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