Somatic MEN1 gene mutation does not contribute
... homo- or hemizygosity. In one case only we identified a possible point mutation in the promoter region. As the patient originated in South America, we cannot exclude a polymorphism. Unfortunately, peripheral blood was not available to discriminate between these two possibilities. Our results are con ...
... homo- or hemizygosity. In one case only we identified a possible point mutation in the promoter region. As the patient originated in South America, we cannot exclude a polymorphism. Unfortunately, peripheral blood was not available to discriminate between these two possibilities. Our results are con ...
Mitochondrial transfer: Ethical, legal and social implications in assisted reproduction
... theory they have more than two biological and social parents. Applying this sensationalism on a broader scale, would children born through embryo donation then have four parents? Nevertheless, researchers have suggested that if a paternal female relative is available and willing to donate oocytes fo ...
... theory they have more than two biological and social parents. Applying this sensationalism on a broader scale, would children born through embryo donation then have four parents? Nevertheless, researchers have suggested that if a paternal female relative is available and willing to donate oocytes fo ...
File
... If these mutagens interact with DNA, they can produce mutations at high rates. Some compounds interfere with base-pairing, increasing the error rate of DNA replication. Others weaken the DNA strand, causing breaks and inversions that produce chromosomal mutations. Cells can sometimes repair the dama ...
... If these mutagens interact with DNA, they can produce mutations at high rates. Some compounds interfere with base-pairing, increasing the error rate of DNA replication. Others weaken the DNA strand, causing breaks and inversions that produce chromosomal mutations. Cells can sometimes repair the dama ...
Case study of primary imatinib resistance and correlation of BCR
... This method is even more sensitive than mutation detection by sequencing of the ABL ATPbinding domain [30] as DNA sequencing can only be used for point mutation detection when the proportion of mutated cells is more than 30% [21]. In cases where the number of mutated cells is less than 30% of the to ...
... This method is even more sensitive than mutation detection by sequencing of the ABL ATPbinding domain [30] as DNA sequencing can only be used for point mutation detection when the proportion of mutated cells is more than 30% [21]. In cases where the number of mutated cells is less than 30% of the to ...
Developmental Effects and Pregnancy Outcomes Associated with Cancer Chemotherapy Use during Pregnancy
... anhydramnios; based on stillbirth and livebirth pregnancies • Apparent rate for any chemotherapy exposure was not different than the prevalence of reductions in amniotic fluid in the general population in US – However, 39% of the reported reductions in amniotic fluid occurred in pregnancies exposed ...
... anhydramnios; based on stillbirth and livebirth pregnancies • Apparent rate for any chemotherapy exposure was not different than the prevalence of reductions in amniotic fluid in the general population in US – However, 39% of the reported reductions in amniotic fluid occurred in pregnancies exposed ...
Co-dominant SCAR marker for detection of the begomovirus
... habrochaites LA0386 (909 bp, EU046611) was identical to the sequence from H24. The sequence for the T0302 marker (789 bp, EU046612) was also obtained for another accession, S. chilense LA2779, that has been used as a source of begomovirusresistance genes (Agrama and Scott, 2006); and the T0302 seque ...
... habrochaites LA0386 (909 bp, EU046611) was identical to the sequence from H24. The sequence for the T0302 marker (789 bp, EU046612) was also obtained for another accession, S. chilense LA2779, that has been used as a source of begomovirusresistance genes (Agrama and Scott, 2006); and the T0302 seque ...
No correlation between phenotype and genotype in boys with a
... mutant alleles of 1:200. We did not find any signs of mosaicism. We believe that this patient is a true hemizygous male, but we cannot prove that some non-mutated cells exist in the brain alone. Although the 816dup7 mutation has not been found in females, it is predicted to cause RS, since a large r ...
... mutant alleles of 1:200. We did not find any signs of mosaicism. We believe that this patient is a true hemizygous male, but we cannot prove that some non-mutated cells exist in the brain alone. Although the 816dup7 mutation has not been found in females, it is predicted to cause RS, since a large r ...
foreign
... Ans. E.coli were allowed to grow on medium containing 15N for many generations so that 15N was incorporated in newly synthesized DNA making it heavy DNA (Nitrogen is important constituent of DNA) = ½ The heavy DNA can be differentiated from light DNA by Caesium Chloride Density Gradient centrifugati ...
... Ans. E.coli were allowed to grow on medium containing 15N for many generations so that 15N was incorporated in newly synthesized DNA making it heavy DNA (Nitrogen is important constituent of DNA) = ½ The heavy DNA can be differentiated from light DNA by Caesium Chloride Density Gradient centrifugati ...
A Mutation in the Eta Subunit of Pyruvate Dehydrogenase
... In previous studies, biochemical and immunologic experiments demonstrated that the proband had variable PD C activity in cells and tissues and that the level of activity corresponded to the amo un t of E] subunit proteins detected by immunoblot analysis (18). Whereas fibroblasts had normal activity ...
... In previous studies, biochemical and immunologic experiments demonstrated that the proband had variable PD C activity in cells and tissues and that the level of activity corresponded to the amo un t of E] subunit proteins detected by immunoblot analysis (18). Whereas fibroblasts had normal activity ...
Pregnancy and Systemic Lupus Erythematosus: at a Single Institution
... Concerning medical therapy, only cyclophosphamide, mycophenolate mofetil, methotrexate, and leflunomide are totally contraindicated drugs both in pregnancy and lactation because of their teratogenicity and embryotoxicity. Minimal data on cyclosporine and TNF alpha inhibitors during pregnancy limit t ...
... Concerning medical therapy, only cyclophosphamide, mycophenolate mofetil, methotrexate, and leflunomide are totally contraindicated drugs both in pregnancy and lactation because of their teratogenicity and embryotoxicity. Minimal data on cyclosporine and TNF alpha inhibitors during pregnancy limit t ...
Print this article - International Journal of Research in Medical
... Hashomer severity score.12-14 The diagnosis of FMF is based on clinical symptoms, and supported by family history and ethnic origin. Since the discovery of MEFV gene, molecular genetic testing is used as a diagnostic helper, particularly in atypical cases. Amyloidosis, the most considerable complica ...
... Hashomer severity score.12-14 The diagnosis of FMF is based on clinical symptoms, and supported by family history and ethnic origin. Since the discovery of MEFV gene, molecular genetic testing is used as a diagnostic helper, particularly in atypical cases. Amyloidosis, the most considerable complica ...
Resources for the map-based cloning of tga1
... segregated for Tga1-maize vs. tga1-teosinte1, was generated by self-pollinating F1 hybrids of the parental stocks. DNAs were isolated using a simplified cTAB method similar to that described elsewhere (Doebley and Stec, 1993, Genetics 134:559-570) except that only one CHCl3 extraction was performed ...
... segregated for Tga1-maize vs. tga1-teosinte1, was generated by self-pollinating F1 hybrids of the parental stocks. DNAs were isolated using a simplified cTAB method similar to that described elsewhere (Doebley and Stec, 1993, Genetics 134:559-570) except that only one CHCl3 extraction was performed ...
Molecular evidence for the existence of additional members of the
... 3720 BA Bilthoven, The Netherlands ...
... 3720 BA Bilthoven, The Netherlands ...
Protein A gene expression is regulated by DNA supercoiling which
... simply due to alterations in the growth rate. We also found that the level of production of the cell-wall-associated protein A was much higher (15-fold) in the presence of novobiocin than in its absence (Fig. 3b). ...
... simply due to alterations in the growth rate. We also found that the level of production of the cell-wall-associated protein A was much higher (15-fold) in the presence of novobiocin than in its absence (Fig. 3b). ...
High-resolution mapping of the leaf rust disease resistance gene Lr1
... Keller 1999). Comparison of the gene composition at orthologous Lrk loci in wheat, barley and rice showed that the high density of genes is conserved at syntenic loci of large and small grass genomes (Feuillet and Keller 1999). Therefore, gene-rich regions in the wheat genome may be amenable to mole ...
... Keller 1999). Comparison of the gene composition at orthologous Lrk loci in wheat, barley and rice showed that the high density of genes is conserved at syntenic loci of large and small grass genomes (Feuillet and Keller 1999). Therefore, gene-rich regions in the wheat genome may be amenable to mole ...
Assembly of additional heterochromatin distinct from centromere
... linear DNA molecules that end with telomere sequences are used (Ikeno et al., 1998). To investigate how transcriptional activity and resulting chromatin structures affect de novo centromere-chromatin assembly and HAC formation, we first constructed the basic alphoid BAC (7C5-basic BAC). It contains ...
... linear DNA molecules that end with telomere sequences are used (Ikeno et al., 1998). To investigate how transcriptional activity and resulting chromatin structures affect de novo centromere-chromatin assembly and HAC formation, we first constructed the basic alphoid BAC (7C5-basic BAC). It contains ...
Characterizing a Lambda Red Recombinase Induced Presumptive
... binding site for inducers such as allolactose and IPTG. The resulting LacI mutant should produce the same amount of lac gene products in the presence and absence of an inducer. A method that is used for performing gene replacements in E.coli is the phage λ Red pathway. The λ Red pathway allows linea ...
... binding site for inducers such as allolactose and IPTG. The resulting LacI mutant should produce the same amount of lac gene products in the presence and absence of an inducer. A method that is used for performing gene replacements in E.coli is the phage λ Red pathway. The λ Red pathway allows linea ...
PPT - Bioinformatics.ca
... – If the gene is not expressed, a mutation in that gene would be less interesting – If the gene is expressed but only from the wild type allele, this might suggest loss-of-function (haploinsufficiency) – If the mutant allele itself is expressed, this might suggest a candidate drug target Module 4 – ...
... – If the gene is not expressed, a mutation in that gene would be less interesting – If the gene is expressed but only from the wild type allele, this might suggest loss-of-function (haploinsufficiency) – If the mutant allele itself is expressed, this might suggest a candidate drug target Module 4 – ...
Frameshift mutations of RIZ, but no point mutations in RIZ1
... fore, the absence of changes in the dinucleotide markers tested here is explainable by the small number of lesions investigated. In gastrointestinal and endometrial carcinomas, MSI has been an important other criterion in lesions with RIZ frameshift mutations (Piao et al., 2000; Chadwick et al., 200 ...
... fore, the absence of changes in the dinucleotide markers tested here is explainable by the small number of lesions investigated. In gastrointestinal and endometrial carcinomas, MSI has been an important other criterion in lesions with RIZ frameshift mutations (Piao et al., 2000; Chadwick et al., 200 ...
Genetic evaluation of suspected osteogenesis imperfecta (OI)
... mutation in the family is known, using DNA extracted from CVS cells or amniocytes. The time from initial tissue sampling to test result is about 7–14 days. ...
... mutation in the family is known, using DNA extracted from CVS cells or amniocytes. The time from initial tissue sampling to test result is about 7–14 days. ...
All research involving recombinant DNA molecules must comply
... 8. Experiments involving more than 10 liters of culture of recombinant DNA organisms or constructs (Section III-D-6*)? 9. Generation of synthetic or recombinant DNA/RNA molecules containing twothirds or less of the genome of any eukaryotic virus (Section III-E-1*)? 10. Viable synthetic or recombinan ...
... 8. Experiments involving more than 10 liters of culture of recombinant DNA organisms or constructs (Section III-D-6*)? 9. Generation of synthetic or recombinant DNA/RNA molecules containing twothirds or less of the genome of any eukaryotic virus (Section III-E-1*)? 10. Viable synthetic or recombinan ...
Induction of fetal demise before abortion
... intentional medical act violated the definitions of the PartialBirth Abortion Ban Act of 2003, which applies only when the fetus has cardiac activity. Although some providers perform amniotomy and divide the cord the day before D&E [70], this intervention has not been described recently in the medic ...
... intentional medical act violated the definitions of the PartialBirth Abortion Ban Act of 2003, which applies only when the fetus has cardiac activity. Although some providers perform amniotomy and divide the cord the day before D&E [70], this intervention has not been described recently in the medic ...
Breastfeeding: Maternal diet and lifestyle
... • Fat synthesis (TG synthesized in cytoplasm and smooth endoplasmic reticulum + precursors imported from maternal circulation): alveolar cells syntesize SCFA ...
... • Fat synthesis (TG synthesized in cytoplasm and smooth endoplasmic reticulum + precursors imported from maternal circulation): alveolar cells syntesize SCFA ...
Warren, ST and Nelson, DL: Trinucleotide repeat expansions in neurological disease. Current Opinion in Neurobiology 3:752-759 (1993).
... more severe, are occasionally seen and are usually paternally inherited [ 531. In one of the first successful attempts at linkage analysis between a restriction fragment length polymorphism and an unmapped human disease, Gusella et al. [ 541 assigned HD to chromosome 4. Intense efforts over the foll ...
... more severe, are occasionally seen and are usually paternally inherited [ 531. In one of the first successful attempts at linkage analysis between a restriction fragment length polymorphism and an unmapped human disease, Gusella et al. [ 541 assigned HD to chromosome 4. Intense efforts over the foll ...