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004 - cse.sc.edu
004 - cse.sc.edu

... Commands for Files • Files are used to store information, for example, data or the results of some analysis. • You will mostly deal with text files • Files on the RCR Alpha are automatically backed up to tape every night. ...
Plant centromeres: structure and control Eric J Richards and R Kelly
Plant centromeres: structure and control Eric J Richards and R Kelly

... for the role of simple DNA repeats in specification of centromere function also comes from structural dissection of human and Drosophila centromeres. Analysis of structurally rearranged human Y chromosomes indicates that the alphoid repeat array is an important functional component of the centromere ...
CapeTownGenomes
CapeTownGenomes

...  New sequencing technologies provide better representation of the genome (by removing cloning steps) and deeper coverage but are harder to assemble because of the short-read lengths.  People now talk about the ‘accessible’ genome for a species. This simply means the output from a reasonably deep s ...
Impact Of The Hypermuscularity GDF8 Gene On Sheep Maternal
Impact Of The Hypermuscularity GDF8 Gene On Sheep Maternal

... The models were applied with Analysis of Variance for prolificacy and lamb 30-days weight traits and with logistic regression for mortality rate trait. Results and Discussion Prolificacy. Regarding the prolificacy trait, results show (Table 1) that the mean level at la Sapinière flock was quite high ...
The Large Loop Repair and Mismatch Repair Pathways
The Large Loop Repair and Mismatch Repair Pathways

... mismatch repair (MMR) and large loop repair (LLR), have been identified previously, but the substrate range of these repair systems has never been defined. To determine the substrates for the MMR and LLR repair pathways, we constructed insertion mutations at HIS4 that form loops of varying sizes whe ...
Polymorphism of FecB Gene in Nine Sheep Breeds or Strains and
Polymorphism of FecB Gene in Nine Sheep Breeds or Strains and

... domain of BMPR-IB, and mutant sheep are characterized by ‘precocious’ differentiation of ovarian follicles, leading to the production of large numbers of ovulatory follicles that are smaller in diameter than ...
Molecular genetics of Rett syndrome and clinical
Molecular genetics of Rett syndrome and clinical

... codons within the last exon may not lead to nonsensemediated mRNA decay [28]. Therefore, the possibility still exists that premature stop codons within exon 4 may produce a truncated form of the protein that retains partial function. A truncated protein that retains the MBD, for instance, may still ...


... obtained from the cross Elgin × E420. The polymorphic fragment generated with primer UBC330 showed a 3:1 segregation ratio but was not linked to the Rps1 locus. Linkage analysis of the five RAPD markers that produce E420-specific bands along with two RFLP markers (Diers et al. 1992) relative to the ...
A Guide to pregnancy
A Guide to pregnancy

... Routine blood count, immunology, biochemistry for kidney and liver function blood tests should be taken. In the presence of a flare there may be evidence of leucopoenia (low white cells). Unreliable features during pregnancy include low platelets and anaemia, as these can occur as part of a normal p ...
- SciTech Connect
- SciTech Connect

... mRNA in the oocyte leads to defects in segment specification, such as defective heads in the embryo.9,10 These and other studies demonstrate that maternally supplied RNAs are required for proper development and can be inherited by the zygote. Studies of sperm composition have shown that despite comm ...
Clin Infect Dis. - Repositorio Académico UPC
Clin Infect Dis. - Repositorio Académico UPC

... 143 stool specimens previously found to be positive for EPEC by PCR of colonies isolated on MacConkey plates of stool culture were analyzed in this study. All stool samples had been tested for specific genes of all 6 pathotypes of diarrheagenic E. coli, including stx1 and sxt2 of Shigatoxin-producin ...
References
References

... traits has been the focus of coadaptation models where specific combinations of demand ...
Interpregnancy weight change and risk of adverse pregnancy
Interpregnancy weight change and risk of adverse pregnancy

... (median 0·7, IQR –0·3 to 1·7) during a mean interpregnancy interval of 24 months (median 20, IQR 13–31). Weight gain between pregnancies decreased with age, education, height, and BMI at the first pregnancy, and was lower in women of Nordic origin; and increased with smoking at the first pregnancy, th ...
Hthsci 2231
Hthsci 2231

... Activity #1: • Under Lab 3 • Click on activity 1 • Click on “Tour of the Basics” • Do all 6 “What is…” exercises Activity #2: • Click on activity 2 • Build your own DNA molecule and transcribe and translate a gene. Activity #3: • Click on activity 3 • Click on “What are Genetic Disorders” o Review l ...
Cytogenetics Cytogenetics
Cytogenetics Cytogenetics

... Example • The first chromosome, long arm, second region of the chromosome, the fourth band of that sub-region ...
Thai Journal of Obstetrics and Gynaecology
Thai Journal of Obstetrics and Gynaecology

Genetic or epigenetic difference causing discordance between
Genetic or epigenetic difference causing discordance between

... dystrophy,34,35 red–green color blindness, Hunter disease,19 and Fabry’s disease.36 For example, in female MZ twins discordant for fragile X syndrome, the length of CGG repeat did not differ, but its methylation status was different. The normal allele of FMR-1 was methylated and inactivated in the a ...
Plants` Epigenetic Secrets
Plants` Epigenetic Secrets

... There are three different types of DNA methylation in plants: CG, CHH (where H is any base except G), and CHG. In Arabidopsis, CG methylation is found on some genes, but primarily on repeat sequences that make up transposons, as well as other repeat sequences in the genome. CHH methylation is found ...
20. Transposable Genetic Elements
20. Transposable Genetic Elements

... Bacterial insertion sequences Insertion sequences, or insertion-sequence (IS) elements, are now known to be segments of bacterial DNA that can move from one position on a chromosome to a different position on the same chromosome or on a different chromosome. When IS elements appear in the middle of ...
Solid Tumour Section Nervous system: Medulloblastoma Atlas of Genetics and Cytogenetics
Solid Tumour Section Nervous system: Medulloblastoma Atlas of Genetics and Cytogenetics

... strongly immuno-reactive for Vimentin. Some tumours are immunoreactive for NSE, Synaptophysine and GSAP. ...
Unbalanced Translocation Breakout
Unbalanced Translocation Breakout

... • Chorionic villus sampling (CVS) – at about 11 to 12 weeks • Amniocentesis – at about 16 to 18 weeks of pregnancy • Non-Invasive Prenatal Testing (NIPT) analyzes cellfree fetal DNA circulating in maternal blood – at about 10 to 22 weeks ...
Semiconservative Replication in the Quasispecies Model
Semiconservative Replication in the Quasispecies Model

... over the gene sequence space. Subsequent studies on the quasispecies model have focused almost exclusively on the error catastrophe [3, 4, 5, 6, 7, 8, 9, 10], though there has also been some work on the dynamical aspects of the equations [11, 12]. More recently, other phase transitions besides the e ...
Localization of a blood pressure QTL on rat chromosome 1 using
Localization of a blood pressure QTL on rat chromosome 1 using

... Quantitative analysis of Sa gene expression. Sa mRNA levels were quantitated using a Hewlett-Packard Scan Jet 3C scanner and NIH Image 1.61 software (Biomedical Magnetic Resonance Laboratory, University of Illinois at UrbanaChampaign). Quantitation was done on the same filter at different time expos ...
Controversias sobre estudios de trombofilia y profilaxis
Controversias sobre estudios de trombofilia y profilaxis

... births in which the intestines herniate through a congenital defect in the abdominal wall on one side of the umbilical cord. That said, the reliability of this risk estimate has been questioned because of use of other drugs, the type of control subjects selected, and failure to definitively confirm ...
Strategies for the molecular genetic manipulation and visualization of the... Penicillium marneffei
Strategies for the molecular genetic manipulation and visualization of the... Penicillium marneffei

... in highly efficient homologous integration (Table 1)(Bugeja et al, 2012). This study describes the development of additional auxotrophic and dominant selectable markers to broaden the options for selection of transformants containing introduced DNA in the type strain of P. marneffei or into clinical ...
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Cell-free fetal DNA

Cell-free fetal DNA (cffDNA) is fetal DNA circulating freely in the maternal blood stream. It can be sampled by venipuncture on the mother. Analysis of cffDNA provides a method of non-invasive prenatal diagnosis.cffDNA originates from the trophoblasts making up the placenta. It is estimated that 2-6% of the DNA in the maternal blood is fetal in origin. The fetal DNA is fragmented and makes its way into the maternal bloodstream via shedding of the placental microparticles into the maternal bloodstream (figure 1). Studies have shown that cffDNA can first be observed as early as 7 weeks gestation, and the amount of cffDNA increases as the pregnancy progresses. cffDNA diminishes quickly after the birth of the baby, so that it is no longer detectable in the maternal blood approximately 2 hours after birth. cffDNA is significantly smaller than the maternal DNA in the bloodstream, with fragments approximately 200bp in size. Many protocols to extract the fetal DNA from the maternal plasma use its size to distinguish it from the maternal DNA.Studies have looked at, and some even optimized, protocols for testing non-compatible RhD factors, sex determination for X-linked genetic disorders and testing for single gene disorders. Current studies are now looking at determining aneuploidies in the developing fetus. These protocols can be done earlier than the current prenatal testing methods, and have no risk of spontaneous abortion, unlike current prenatal testing methods. Non-invasive prenatal diagnosis (NIPD) has been implemented in the UK and parts of the US; it has clear benefits above the standard tests of chorionic villi sample (CVS) and amniocentesis which have procedure-related miscarriage risks of about 1 in 100 pregnancies and 1 in 200 pregnancies, respectively.As a method of prenatal diagnosis, cell-free fetal DNA techniques share the same ethical and practical issues, such as the possibility of prenatal sex discernment and sex selection.
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