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Philippine Society of Maternal and Fetal Medicine
Philippine Society of Maternal and Fetal Medicine

... provide maximum sensitivity. Pregnancy brings about changes in insulin sensitivity, degradation and activity, and it has been recognized that non-pregnant norms for oral glucose tolerance might not be valid during pregnancy. In 1964, O’Sullivan and Mahan published their landmark study using a 3-hour ...
An Artist in Gene Editing - Max-Planck
An Artist in Gene Editing - Max-Planck

... Center: The two RNA molecules can be merged – this makes it easier to use CRISPR-Cas9 for research purposes. Bottom: The CRISPR-RNA, together with its recognition sequence, attaches itself to a suitable DNA sequence, identifying the interface for Cas9. This is how genes can be cut out or new ones in ...
Messenger RNA
Messenger RNA

... Genes contain coded DNA instructions that tell cells how to build proteins. The first step in decoding these genetic instructions is to copy part of the base sequence from DNA into RNA, which then uses these instructions to direct the production of protein which help to establish an organism’s chara ...
Table VI. Diagnostic laboratory studies for PBD Test Metabolite/gene
Table VI. Diagnostic laboratory studies for PBD Test Metabolite/gene

... ketogenic diet, nonfasting or hemolyzed samples, normal in RCDP1 Phytanic acid is normal in newborns and accumulates only through dietary intake Greatest reduction in RCDP1 Adjunct to VLCFA and plasmalogen testing Adjunct to VLCFA and plasmalogen testing Confirmatory testing, also allows more comple ...
Pospartum Hemorrhage (PPH)
Pospartum Hemorrhage (PPH)

... vaginal delivery or 1000 mL after Cesarean delivery. Decline in hematocrit of more than 10% may be used. Severe PPH is greater than or equal to 1000 ml within 24 hours after delivery. Primary postpartum hemorrhage occurs in first 24 hours, while secondary hemorrhage occurs after 24 hours and before ...
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(lectures 24

... Hawaiian species of the genus Drosophila. Being dipterans (flies) they have giant salivary gland chromosomes which are not only polytene (multiple stranded) and can have many bands identified on them by staining for DNA, but also the two homologues are paired in this larval salivary gland! (This mus ...
Hydrogen autotrophy of Nocardia opaca strains is
Hydrogen autotrophy of Nocardia opaca strains is

... megaplasmids in each of the two wild-type strains of N. opaca studied. This success is also due to the application of an alternative method for preparation of DNA in agarose plugs containing the bacterial cells. For unknown reasons this method was found not to be suited to detecting the cccDNA plasm ...
DNA Lesson 2 Guide
DNA Lesson 2 Guide

... 2. Build the DNA on top of the gene strip. Check that your nucleotides match the strip exactly. The bottom strand of DNA is the gene. 3. Notice the DNA nucleotides on the bottom of your gene strip are marked in groups of 3 with dark gray boxes. A group of 3 nucleotides is called a codon. ...
Powerpoint template for scientific posters (Swarthmore
Powerpoint template for scientific posters (Swarthmore

... Meiothermus ruber Genome Annotation Project is a network of regional 2-year and 4-year colleges/universities that are collaborating to annotate the ~3000 putative coding regions identified in the initial automated gene-calling analysis of the Meiothermus ruber genome. In this project, 11 students fr ...
Human Inheritance Patterns
Human Inheritance Patterns

... The couple was no longer concerned about their risk of having a child with CF based on these results. To support their belief, they had a healthy 5-year-old son who had a negative sweat test at the age of 4 months. The counselor explained the need to review the records and scheduled a followup appoi ...
Perl Laboratory Study Guide – Section I
Perl Laboratory Study Guide – Section I

... Add a line that takes an output filename from the command line. For example, the command line should be something like: perl ex6-1.pl infile.fasta outfile.txt At the end of the script, add a couple of lines that open, and write to, a results file. Below is an example of what writing to a file might ...
Smith, GF and Warren, ST: The biology of Down syndrome. Annals of the New York Academy of Science 450: 1-9 (1985).
Smith, GF and Warren, ST: The biology of Down syndrome. Annals of the New York Academy of Science 450: 1-9 (1985).

... will likely be quite small when one considers that monosomy for a single gene (i.e., a heterozygote for a metabolic disorder) is phenotypically normal. Therefore, it is not unlikely that for many genes, the presence of an extra copy would likewise be of little consequence. Thus the remaining genes m ...
Chapter 12 Inheritance Patterns and Human Genetics
Chapter 12 Inheritance Patterns and Human Genetics

... E. Edward’s Syndrome A. Trisomy 18 B. Most children only live a few months C. All major organs affected ...
Molecular Marker Technology for Cotton Plant Improvement
Molecular Marker Technology for Cotton Plant Improvement

... components by finding DNA markers closely linked to each trait, it might be possible to manipulate them with efficiency for single gene traits. This would help the advancement of breeding material through consistent progress. DNA markers technology has provided plant breeders with a tool to select d ...
DNA Mutation and Repair
DNA Mutation and Repair

... • Each cell division = copy 6 x 109 bases • Average replication error rate = ~10-10/nucleotide • Any given gene may be the site of ~109 mutations when considering all cells and cell divisions • Human heterozygosity - measure of allelic difference within an individual • ~0.08% (~1/1250 bp) ...
genotyping arabidopsis - STLCC.edu :: Users` Server
genotyping arabidopsis - STLCC.edu :: Users` Server

... the buffer because magnesium acts as a co-enzyme, fitting into the three dimensional structure of the protein to activate it. Too much magnesium can reduce the ability of the polymerase to stay attached to the template (i.e., fidelity is decreased), so the right concentration (1.0 – 3.0 mM) is cruci ...
question 2 - Curriculum Development
question 2 - Curriculum Development

... Telophase I Telophase 2 ...
Highly conserved features of DNA binding between two divergent
Highly conserved features of DNA binding between two divergent

... cerevisiae transcription factor Bas1p has revealed that mutations in the tryptophan residues strongly impair function of the protein both in vitro and in vivo (8). An interesting exception to this rule is the CDC5 subfamily, which contains the Cef1p protein from S.cerevisiae (9). Proteins from this ...
A Rapid Screening Method to Detect Nonsense and Frameshift
A Rapid Screening Method to Detect Nonsense and Frameshift

... normal DNA inserts varied, especially in comparison to the lighter blue color obtained from the mutant inserts. Although it was not difficult to differentiate colors among colonies on a single plate, comparisons from one plate to another were difficult to interpret. Colors obtained from samples with ...
Ribosomal DNA sequences reveal gregarine pathogens
Ribosomal DNA sequences reveal gregarine pathogens

... The gene coding for the small subunit of ribosomal RNA (SSU RNA) is the most intensively sequenced marker for phylogenetic studies in all groups of organisms, including mites. Newly obtained sequence data can be quickly and easily compared with all published sequences of this marker deposited in Gen ...
Tu et al. Respond to “Barker Meets Simpson”
Tu et al. Respond to “Barker Meets Simpson”

... is the product of the interaction between genetic and environmental factors. Thus, while many studies exploring the fetal origins of adult disease hypothesis use birth weight as a marker for prenatal factors responsible for size at birth, birth weight is also a variable that captures those other gen ...
Breeding desired quality wheat by reverse genetics
Breeding desired quality wheat by reverse genetics

... test (SKCS) showed phenotypes with harder or softer grain character were identified in these mutants. A recent survey by Pickering and Bhave (2007) of the genotypes of Australian hard wheats noted the presence of only the Pina-D1b and Pinb-D1b types. The TILLING work allows for the expansion of the ...
Cytogenetic and AZF microdeletions on the Y chromosome of
Cytogenetic and AZF microdeletions on the Y chromosome of

... Master Mix reactions are shown. Each Multiplex Master Mix is used for amplification of Male Genomic DNA (MD115A) samples (lanes 1, 3, 5, 8, 10) and a representative sample containing (lanes 2, 4, 7, 9, 11). Bands resulting from the amplification of the positive Male Genomic DNA control are compared ...
Slide 1
Slide 1

... 1) Unordered traits, such that there is no structure in their states. Two states can only be identical or different. The most important example is a nucleotide site, which can accept 4 states - A, T, G, C one cannot usually say that A is more similar to T than to G. Many loci are unordered traits, i ...
Lecture 11-POSTED-BISC441-2012
Lecture 11-POSTED-BISC441-2012

... pregnancy and reduces maternal food intake (via nausea), which stimulates placental growth?? Might protection from toxins, pathogens be a byproduct of this system?? Might variation in aversion to different foods be a non-adaptive byproduct of general nausea?? ...
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Cell-free fetal DNA

Cell-free fetal DNA (cffDNA) is fetal DNA circulating freely in the maternal blood stream. It can be sampled by venipuncture on the mother. Analysis of cffDNA provides a method of non-invasive prenatal diagnosis.cffDNA originates from the trophoblasts making up the placenta. It is estimated that 2-6% of the DNA in the maternal blood is fetal in origin. The fetal DNA is fragmented and makes its way into the maternal bloodstream via shedding of the placental microparticles into the maternal bloodstream (figure 1). Studies have shown that cffDNA can first be observed as early as 7 weeks gestation, and the amount of cffDNA increases as the pregnancy progresses. cffDNA diminishes quickly after the birth of the baby, so that it is no longer detectable in the maternal blood approximately 2 hours after birth. cffDNA is significantly smaller than the maternal DNA in the bloodstream, with fragments approximately 200bp in size. Many protocols to extract the fetal DNA from the maternal plasma use its size to distinguish it from the maternal DNA.Studies have looked at, and some even optimized, protocols for testing non-compatible RhD factors, sex determination for X-linked genetic disorders and testing for single gene disorders. Current studies are now looking at determining aneuploidies in the developing fetus. These protocols can be done earlier than the current prenatal testing methods, and have no risk of spontaneous abortion, unlike current prenatal testing methods. Non-invasive prenatal diagnosis (NIPD) has been implemented in the UK and parts of the US; it has clear benefits above the standard tests of chorionic villi sample (CVS) and amniocentesis which have procedure-related miscarriage risks of about 1 in 100 pregnancies and 1 in 200 pregnancies, respectively.As a method of prenatal diagnosis, cell-free fetal DNA techniques share the same ethical and practical issues, such as the possibility of prenatal sex discernment and sex selection.
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