SYBR Green I Nucleic Acid Gel Stain
... SYBR Green I Stain is a highly sensitive fluorescent stain for detecting nucleic acids in agarose and polyacrylamide gels. The dye exhibits a preferential affinity for DNA and its fluorescent signal is greatly enhanced when bound to DNA (more than an order of magnitude greater than the fluorescent e ...
... SYBR Green I Stain is a highly sensitive fluorescent stain for detecting nucleic acids in agarose and polyacrylamide gels. The dye exhibits a preferential affinity for DNA and its fluorescent signal is greatly enhanced when bound to DNA (more than an order of magnitude greater than the fluorescent e ...
Classification of Centers for Disease Control Group Eugonic
... group of Gram-negative saccharolytic organisms predominantly recovered from human wounds resulting from dog or cat bites. EF-4 isolates are considered to be commensal organisms found in the oral cavity of dogs and cats, although systemic infections in humans and animals have also been reported (Gani ...
... group of Gram-negative saccharolytic organisms predominantly recovered from human wounds resulting from dog or cat bites. EF-4 isolates are considered to be commensal organisms found in the oral cavity of dogs and cats, although systemic infections in humans and animals have also been reported (Gani ...
Mitochondrial diseases and the role of the yeast models
... (Johannsen & Ravussin, 2009). They are not the topics of this review and some will be treated in other chapters of this issue. Here, we will concentrate on diseases due to mutations that directly affect mitochondrial genes or gene expression in a monogenic manner. Because mitochondrial biogenesis re ...
... (Johannsen & Ravussin, 2009). They are not the topics of this review and some will be treated in other chapters of this issue. Here, we will concentrate on diseases due to mutations that directly affect mitochondrial genes or gene expression in a monogenic manner. Because mitochondrial biogenesis re ...
Myriad--Ambry -- Final Version of Ambry Preliminary Injunction
... million dollars to implement this discovery and create a molecular diagnostic test for hereditary breast and ovarian cancer related to the BRCA1 and BRCA2 genes that has revolutionized patient care and provided medical diagnosis and treatment options never thought possible. This investment has yield ...
... million dollars to implement this discovery and create a molecular diagnostic test for hereditary breast and ovarian cancer related to the BRCA1 and BRCA2 genes that has revolutionized patient care and provided medical diagnosis and treatment options never thought possible. This investment has yield ...
Microbial Ecology: Where are we now?
... undesired enzymatic digestion of secondary single stranded molecular structures. Formation of ‘pseudo-T-RFs’ result in additional peaks that may lead to over estimation of microbial diversity and the need to generate clone libraries due to inaccessibility of sequences (Egert and Friedrich 2003). Typ ...
... undesired enzymatic digestion of secondary single stranded molecular structures. Formation of ‘pseudo-T-RFs’ result in additional peaks that may lead to over estimation of microbial diversity and the need to generate clone libraries due to inaccessibility of sequences (Egert and Friedrich 2003). Typ ...
PowerPoint Presentation - Meiosis
... • Intimate contact provides route for infection by parasites (AIDS, syphillis, etc.) • Genetic costs: in sex, we pass on only half of genes to offspring. • Males are an expensive luxury - in most species they contribute little to rearing offspring. ...
... • Intimate contact provides route for infection by parasites (AIDS, syphillis, etc.) • Genetic costs: in sex, we pass on only half of genes to offspring. • Males are an expensive luxury - in most species they contribute little to rearing offspring. ...
Structure, expression and chromosomal location of the Oct
... the relevant parental t-haplotypes. This lack of polymorphism was not unexpected because all t-haplotypes are generally believed to be descended from a single ancestral chromosome. Another approach to examine the relationship between Oct-4 and t 12 is to analyze the genomic structure and sequence of ...
... the relevant parental t-haplotypes. This lack of polymorphism was not unexpected because all t-haplotypes are generally believed to be descended from a single ancestral chromosome. Another approach to examine the relationship between Oct-4 and t 12 is to analyze the genomic structure and sequence of ...
Meiosis - TeacherWeb
... • Large amounts of energy required to find a mate and do the mating: specialized structures and behavior required • Intimate contact provides route for infection by parasites (AIDS, syphillis, etc.) • Genetic costs: in sex, we pass on only half of genes to offspring. • Males - in most species they c ...
... • Large amounts of energy required to find a mate and do the mating: specialized structures and behavior required • Intimate contact provides route for infection by parasites (AIDS, syphillis, etc.) • Genetic costs: in sex, we pass on only half of genes to offspring. • Males - in most species they c ...
KAIE LOKK Comparative genome-wide DNA methylation
... the proportion of methylated CpG sites can vary greatly over a genome. Repeated sequences tend to be the most heavily methylated, while CpG-rich regulatory regions are almost exclusively unmethylated in all human tissues. As DNA methylation is vital for the normal functioning of organism, changes in ...
... the proportion of methylated CpG sites can vary greatly over a genome. Repeated sequences tend to be the most heavily methylated, while CpG-rich regulatory regions are almost exclusively unmethylated in all human tissues. As DNA methylation is vital for the normal functioning of organism, changes in ...
Development of Co-Dominant Amplified Polymorphic Sequence
... Resistance to M. grisea isolate PO6-6 in RIL29 is located on chromosome 11. Both Pi1 and Pi7(t) initially were mapped using a chromosome marker set derived from the mapping population developed at Cornell in the late 1980s and early 1990s (17). However, all markers used in this report are derived fr ...
... Resistance to M. grisea isolate PO6-6 in RIL29 is located on chromosome 11. Both Pi1 and Pi7(t) initially were mapped using a chromosome marker set derived from the mapping population developed at Cornell in the late 1980s and early 1990s (17). However, all markers used in this report are derived fr ...
reproductive cell fate transition in plants - Development
... during MMC specification (Olmedo-Monfil et al., 2010; Singh et al., 2011) and later for the initiation of female gametophyte development (Tucker et al., 2012). In addition, SMC differentiation is characterized by elevated transcriptional levels for many of the enzymes that participate in epigenetic ...
... during MMC specification (Olmedo-Monfil et al., 2010; Singh et al., 2011) and later for the initiation of female gametophyte development (Tucker et al., 2012). In addition, SMC differentiation is characterized by elevated transcriptional levels for many of the enzymes that participate in epigenetic ...
Translational Repression of C. elegans p53 by GLD
... tumors have defects in upstream signaling components of the p53 pathway such as inactivation of the positive regulators ARF or CHK2 (Sharpless and DePinho, 1999; Bartek and Lukas, 2003), or overexpression of the negative regulator Mdm2 (Freedman et al., 1999). For those tumors that retain functional ...
... tumors have defects in upstream signaling components of the p53 pathway such as inactivation of the positive regulators ARF or CHK2 (Sharpless and DePinho, 1999; Bartek and Lukas, 2003), or overexpression of the negative regulator Mdm2 (Freedman et al., 1999). For those tumors that retain functional ...
Systematic and Applied Microbiology - digital
... analyzed in an agarose gel (Figure 2). All the tyramine producer strains gave a 1.9 kb DNA fragment. The strain CECT 964 did not amplify the DNA fragment. These results agreed with those obtained from the chromatographic analysis. In summary, all the E. faecium strains analysed were unexpectedly abl ...
... analyzed in an agarose gel (Figure 2). All the tyramine producer strains gave a 1.9 kb DNA fragment. The strain CECT 964 did not amplify the DNA fragment. These results agreed with those obtained from the chromatographic analysis. In summary, all the E. faecium strains analysed were unexpectedly abl ...
Chapter 1 - Institut Montefiore
... • The human genome consists of about 3 ×109 base pairs and contains about 30,000 genes • Much of the DNA is either in introns or in intragenic regions • Cells containing 2 copies of each chromosome are called diploid (most human cells). Cells that contain a single copy are called haploid. • Humans h ...
... • The human genome consists of about 3 ×109 base pairs and contains about 30,000 genes • Much of the DNA is either in introns or in intragenic regions • Cells containing 2 copies of each chromosome are called diploid (most human cells). Cells that contain a single copy are called haploid. • Humans h ...
Curr. Microbiol. 42
... 5 (Table 2) detected one that yielded an amplicon specific to cry8Ba. This same field-collected strain must be novel because it did not react with a cry8Ba-specific pair of primers [2]. A new gene will be detected by propagating a specific amplicon(s) differing from the standard pattern for cry7 (Fi ...
... 5 (Table 2) detected one that yielded an amplicon specific to cry8Ba. This same field-collected strain must be novel because it did not react with a cry8Ba-specific pair of primers [2]. A new gene will be detected by propagating a specific amplicon(s) differing from the standard pattern for cry7 (Fi ...
Missouri Western State University
... A Burnt Pancake Model (cont.) We want the most efficient way to change the stack. Below we use only three flips instead of five. ...
... A Burnt Pancake Model (cont.) We want the most efficient way to change the stack. Below we use only three flips instead of five. ...
FEMS Microbiology Letters
... PFO and tetrameric POR/VOR/KOR. Sequence alignments performed for only some of these oxidoreductase groups are scattered in the literature [2,16^18]. Most of the tetrameric and dimeric oxidoreductase-containing species do not possess a homodimeric PFO. Campylobacter jejuni is an exception as it cont ...
... PFO and tetrameric POR/VOR/KOR. Sequence alignments performed for only some of these oxidoreductase groups are scattered in the literature [2,16^18]. Most of the tetrameric and dimeric oxidoreductase-containing species do not possess a homodimeric PFO. Campylobacter jejuni is an exception as it cont ...
reproductive cell fate transition in plants - Development
... ago9-4, sgs3-11 and rdr6-2 (Olmedo-Monfil et al., 2010), which were grown at 23°C in a growth incubator (Percival). The GFP lines shown Fig. 2 and supplementary material Fig. S1 are the following: HTR5-GFP is pHTR5::HTR5-GFP and HTR8-GFP is pHTR8::HTR8-CFP (Ingouff et al., 2010); H2A.Z-GFP is pHTA11 ...
... ago9-4, sgs3-11 and rdr6-2 (Olmedo-Monfil et al., 2010), which were grown at 23°C in a growth incubator (Percival). The GFP lines shown Fig. 2 and supplementary material Fig. S1 are the following: HTR5-GFP is pHTR5::HTR5-GFP and HTR8-GFP is pHTR8::HTR8-CFP (Ingouff et al., 2010); H2A.Z-GFP is pHTA11 ...
Sequence requirements for function of the
... gene loci (Asano and Wharton, 1999; Calvi et al., 1998; Royzman et al., 1999). The data demonstrate that Drosophila chorion gene amplification uses evolutionarily conserved machinery for initiation; however, some mechanism must exist to uniquely mark the chorion gene loci origins for activation duri ...
... gene loci (Asano and Wharton, 1999; Calvi et al., 1998; Royzman et al., 1999). The data demonstrate that Drosophila chorion gene amplification uses evolutionarily conserved machinery for initiation; however, some mechanism must exist to uniquely mark the chorion gene loci origins for activation duri ...
The genomic landscape of meiotic crossovers and gene
... Sequencing and genotyping of meiotic tetrads We constructed F1 hybrids of the A. thaliana accessions Columbia (Col) and Landsberg erecta (Ler) in the quartet1 (qrt1) −/− background (Preuss et al., 1994) and crossed individual pollen tetrads to a male sterile EMS mutant of the Cape Verde Island (Cvi) ...
... Sequencing and genotyping of meiotic tetrads We constructed F1 hybrids of the A. thaliana accessions Columbia (Col) and Landsberg erecta (Ler) in the quartet1 (qrt1) −/− background (Preuss et al., 1994) and crossed individual pollen tetrads to a male sterile EMS mutant of the Cape Verde Island (Cvi) ...
Base Composition and Translational Selection are Insufficient to
... linear ~10kb genome that is expressed as a polyprotein, luteoviruses contain a linear genome of 5.3- ...
... linear ~10kb genome that is expressed as a polyprotein, luteoviruses contain a linear genome of 5.3- ...
Polygenes and Modifier Genes for Tetracycline and
... ‘double dose effect’ does not occur. The tet in 6T20, MT93 and TM14 DNA preparations has additive effects with the mtr in 4B10, increasing its level of resistance from 0.3 to 0.6 ,ug ml-l. The same effect occurs using mtr DNA (4B10 or MT93) in combination with tet (6T20). The tet tern in TM14 DNA ha ...
... ‘double dose effect’ does not occur. The tet in 6T20, MT93 and TM14 DNA preparations has additive effects with the mtr in 4B10, increasing its level of resistance from 0.3 to 0.6 ,ug ml-l. The same effect occurs using mtr DNA (4B10 or MT93) in combination with tet (6T20). The tet tern in TM14 DNA ha ...
Phytopathology
... is an emerging methodology useful for identification of DNA fragments and may be applicable for rapid identification and detection of plant pathogens associated with plants (20,21,26). An array of species-specific oligonucleotide probes representing the various pathogens of potato, built on a solid ...
... is an emerging methodology useful for identification of DNA fragments and may be applicable for rapid identification and detection of plant pathogens associated with plants (20,21,26). An array of species-specific oligonucleotide probes representing the various pathogens of potato, built on a solid ...
Extrachromosomal DNA
Extrachromosomal DNA is any DNA that is found outside of the nucleus of a cell. It is also referred to as extranuclear DNA or cytoplasmic DNA. Most DNA in an individual genome is found in chromosomes but DNA found outside of the nucleus also serves important biological functions.In prokaryotes, nonviral extrachromosomal DNA is primarily found in plasmids whereas in eukaryotes extrachromosomal DNA is primarily found in organelles. Mitochondrial DNA is a main source of this extrachromosomal DNA in eukaryotes. Extrachromosomal DNA is often used in research of replication because it is easy to identify and isolate.Extrachromosomal DNA was found to be structurally different from nuclear DNA. Cytoplasmic DNA is less methylated than DNA found within the nucleus. It was also confirmed that the sequences of cytoplasmic DNA was different from nuclear DNA in the same organism, showing that cytoplasmic DNAs are not simply fragments of nuclear DNA.In addition to DNA found outside of the nucleus in cells, infection of viral genomes also provides an example of extrachromosomal DNA.