UCSC genome support forum
... 2.2. Enter your assembly of choice and enter your coordinates in the “search term” box 2.3. Click the “submit” button 3. In the blue navigation bar at the top of the screen, click “View/DNA” 4. Click the “get DNA” button 5. Copy the DNA sequence 6. Navigate to http://genome.ucsc.edu/cgi-bin/hgBlat 7 ...
... 2.2. Enter your assembly of choice and enter your coordinates in the “search term” box 2.3. Click the “submit” button 3. In the blue navigation bar at the top of the screen, click “View/DNA” 4. Click the “get DNA” button 5. Copy the DNA sequence 6. Navigate to http://genome.ucsc.edu/cgi-bin/hgBlat 7 ...
PCR Clean-up Kit / 96-well PCR Clean
... cosmid DNA (per well) from up to 1.3 ml of bacterial culture. This method is based on a modified alkaline lysis of the bacteria in combination with the selective binding of the plasmid DNA to a special silica membrane. The purified plasmid or cosmid DNA is free from protein, genomic DNA and RNA and ...
... cosmid DNA (per well) from up to 1.3 ml of bacterial culture. This method is based on a modified alkaline lysis of the bacteria in combination with the selective binding of the plasmid DNA to a special silica membrane. The purified plasmid or cosmid DNA is free from protein, genomic DNA and RNA and ...
My PP Genetics
... Each codon represents an amino acid. These are strung together to make a protein. Translation uses transfer RNA (tRNA) to transfer amino acids to the protein being assembled. Copyright © 2007 Pearson Education, Inc., publishing as Pearson Addison-Wesley ...
... Each codon represents an amino acid. These are strung together to make a protein. Translation uses transfer RNA (tRNA) to transfer amino acids to the protein being assembled. Copyright © 2007 Pearson Education, Inc., publishing as Pearson Addison-Wesley ...
lntraclonal mating in Trypanosoma brucei is
... 1995). Other lower eukaryotes have multiple mating types, determined by loci which encode or control production of specific pheromones ; such systems ensure that mating occurs only between organisms of different ...
... 1995). Other lower eukaryotes have multiple mating types, determined by loci which encode or control production of specific pheromones ; such systems ensure that mating occurs only between organisms of different ...
Repetitive complete hydatidiform mole can be biparental in origin
... pregnancy, is associated with the presence of two paternal genomes and thus involves imprinted genes, that is genes which are normally only expressed from the maternally or paternally derived allele. Further evidence that the trophoblastic hyperplasia typical of molar pregnancies results from increa ...
... pregnancy, is associated with the presence of two paternal genomes and thus involves imprinted genes, that is genes which are normally only expressed from the maternally or paternally derived allele. Further evidence that the trophoblastic hyperplasia typical of molar pregnancies results from increa ...
Structure and function of the GINS complex, a key component of the
... but two essential proteins, Sld2 and Sld3, have been shown to comprise the minimal set required for replication initiation [21,22]. Phosphorylation of Sld3 by S-CDK on Thr600 and Ser622 is essential for cell viability, substituting these two residues with non-phosphorylatable alanine residues blocks ...
... but two essential proteins, Sld2 and Sld3, have been shown to comprise the minimal set required for replication initiation [21,22]. Phosphorylation of Sld3 by S-CDK on Thr600 and Ser622 is essential for cell viability, substituting these two residues with non-phosphorylatable alanine residues blocks ...
Molecular Diagnostics in Clinical Microbiology
... PCR, based on the detection of the methicillin resistance determinant mecA, is still considered to be the gold standard molecular-diagnostic tool for MRSA. PCR assays which detect a single target (mecA) are both robust and easy to perform (6). However, amplification inhibition may lead to false-nega ...
... PCR, based on the detection of the methicillin resistance determinant mecA, is still considered to be the gold standard molecular-diagnostic tool for MRSA. PCR assays which detect a single target (mecA) are both robust and easy to perform (6). However, amplification inhibition may lead to false-nega ...
Tutorial: Mendelian Genetics - Integrated DNA Technologies
... Today, when the word “genetics” is mentioned the mind is at once occupied with terms like cloning, PCR, the genome project, and genomics. Just a few decades ago, however, the word genetics conjured up a very different set of terms including crossing, segregation, Punnett square, and binomial expansi ...
... Today, when the word “genetics” is mentioned the mind is at once occupied with terms like cloning, PCR, the genome project, and genomics. Just a few decades ago, however, the word genetics conjured up a very different set of terms including crossing, segregation, Punnett square, and binomial expansi ...
Ancient DNA Laboratory Guidelines
... aDNA Laboratory, simply remove the trash and place it in the hallway outside the room. This should be done every day that the aDNA Laboratory is used, even if projects require the use of the aDNA Laboratory over several days. Publishable aDNA Laboratory Results “Ancient” DNA studies over the past f ...
... aDNA Laboratory, simply remove the trash and place it in the hallway outside the room. This should be done every day that the aDNA Laboratory is used, even if projects require the use of the aDNA Laboratory over several days. Publishable aDNA Laboratory Results “Ancient” DNA studies over the past f ...
Transgenic Plastids in Basic Research and Plant Biotechnology
... transformation event involves the change of only a single (or at most a few) out of several thousand plastid genome copies in a leaf cell. During subsequent cell and organelle divisions, the presence of high concentrations of the selecting antibiotic favors multiplication of chloroplasts containing ...
... transformation event involves the change of only a single (or at most a few) out of several thousand plastid genome copies in a leaf cell. During subsequent cell and organelle divisions, the presence of high concentrations of the selecting antibiotic favors multiplication of chloroplasts containing ...
Bacteroides mobilizable and conjugative genetic elements
... rteA and rteB have been shown to be in the same operon as tetQ, production of these proteins is presumably due to translational coupling one ribosomes begin to translate the operon mRNA. RteA and RteB have no role in tetracycline regulation of operon expression. This was somewhat surprising because ...
... rteA and rteB have been shown to be in the same operon as tetQ, production of these proteins is presumably due to translational coupling one ribosomes begin to translate the operon mRNA. RteA and RteB have no role in tetracycline regulation of operon expression. This was somewhat surprising because ...
345 - Timstar
... that alteration may not necessarily change the amino acid sequence of the subsequent protein produced due to degeneracy of the genetic code. On the other hand, SNPs that do not fall into a coding sequence may still affect that region of DNA in such a way by inducing gene splicing, transcription fact ...
... that alteration may not necessarily change the amino acid sequence of the subsequent protein produced due to degeneracy of the genetic code. On the other hand, SNPs that do not fall into a coding sequence may still affect that region of DNA in such a way by inducing gene splicing, transcription fact ...
Antisense Transcript and RNA Processing
... was required for viability but could not produce stable atpB transcripts. Based on strand-specific RT-PCR, S1 nuclease protection, and RNA gel blots, evidence was obtained that the PSþ genome stabilizes atpB mRNA by generating an atpB antisense transcript, which attenuates the degradation of the pol ...
... was required for viability but could not produce stable atpB transcripts. Based on strand-specific RT-PCR, S1 nuclease protection, and RNA gel blots, evidence was obtained that the PSþ genome stabilizes atpB mRNA by generating an atpB antisense transcript, which attenuates the degradation of the pol ...
2q13 microduplications
... When the sperm and egg cells join they form a single cell and this cell must continuously make copies of itself (and all its genetic material) in order to produce the billions of cells that are necessary for human growth and development. Sometimes during the formation of the egg or sperm cells or du ...
... When the sperm and egg cells join they form a single cell and this cell must continuously make copies of itself (and all its genetic material) in order to produce the billions of cells that are necessary for human growth and development. Sometimes during the formation of the egg or sperm cells or du ...
A fost luat în studiu caracterul multifoliolar deoarece acest caracter
... trait was not observed in any of the descendants. Further on it was emphasized for 14% of the descendants: the number of leaflets per each leave varied between 4 and 6. From the F2 individuals, which were divided into two categories depending on the presence or absence of multileaflet trait, the B1 ...
... trait was not observed in any of the descendants. Further on it was emphasized for 14% of the descendants: the number of leaflets per each leave varied between 4 and 6. From the F2 individuals, which were divided into two categories depending on the presence or absence of multileaflet trait, the B1 ...
HUMAN CHROMOSOMES
... The somatic tissues of females are thus said to be mosaic because they represent the contribution of genes from different X chromosomes. In each somatic cell the genes in only one X chromosome will be expressed, but the X chromosome that is genetically active will differ from cell to cell. The m ...
... The somatic tissues of females are thus said to be mosaic because they represent the contribution of genes from different X chromosomes. In each somatic cell the genes in only one X chromosome will be expressed, but the X chromosome that is genetically active will differ from cell to cell. The m ...
PDF - Journal of Genomics
... acids and carbohydrates that are abundant in their phloem-based diet or produced by the host. Genomic evidence suggests that several amino acid biosynthetic pathways are shared between aphid and Buchnera, providing the aphid the ability to regulate the endosymbiont’s metabolism (16). No aphid specie ...
... acids and carbohydrates that are abundant in their phloem-based diet or produced by the host. Genomic evidence suggests that several amino acid biosynthetic pathways are shared between aphid and Buchnera, providing the aphid the ability to regulate the endosymbiont’s metabolism (16). No aphid specie ...
PDF
... was unaffected even by continuous treatments at the above concentrations, so that initiated buds always developed the normal tubular appearance characteristic of later stages. This supports the earlier suggestion (Clarkson & Wolpert, 1967) that bud elongation must be interpreted in terms of tissue m ...
... was unaffected even by continuous treatments at the above concentrations, so that initiated buds always developed the normal tubular appearance characteristic of later stages. This supports the earlier suggestion (Clarkson & Wolpert, 1967) that bud elongation must be interpreted in terms of tissue m ...
1. Introduction
... two nearby single strand breaks in the DNA duplex of one or more chromosomes. Breaks can be generated by exogenous (e.g., ionizing radiations) or endogenous (e.g., reactive oxygen species) agents. Because “open” DNA ends cannot ligate to each other without catalysis by specific enzymes, the cellular ...
... two nearby single strand breaks in the DNA duplex of one or more chromosomes. Breaks can be generated by exogenous (e.g., ionizing radiations) or endogenous (e.g., reactive oxygen species) agents. Because “open” DNA ends cannot ligate to each other without catalysis by specific enzymes, the cellular ...
Microarrays
... 0.1 ng gDNA and 104 cfu/mL in a pure culture. Even though the authors reported reliable detection from binary and ternary mixtures, data on the relative sensitivity of the microarray have not been provided. Analysis of mock spiked PIF samples revealed sensitivity in the range of 1–10 cfu/25 g sample ...
... 0.1 ng gDNA and 104 cfu/mL in a pure culture. Even though the authors reported reliable detection from binary and ternary mixtures, data on the relative sensitivity of the microarray have not been provided. Analysis of mock spiked PIF samples revealed sensitivity in the range of 1–10 cfu/25 g sample ...
Ribotyping of Clostridium perfringens from industrially produced
... DNA analysis (ribotyping), a method which analyses restriction fragment length polymorphisms in the chromosomal genes that encode rRNA, has been shown to be useful for microbial species identification and subtyping. Methods and Results: The current study has used ribotyping to examine 111 Cl. perfri ...
... DNA analysis (ribotyping), a method which analyses restriction fragment length polymorphisms in the chromosomal genes that encode rRNA, has been shown to be useful for microbial species identification and subtyping. Methods and Results: The current study has used ribotyping to examine 111 Cl. perfri ...
Towards Programmable Molecular Machines
... We believe theoretical techniques and algorithmic analysis can offer significant insight for the development of these machines. Our goal in this paper is to take one class of molecular machines and formally show how they can be used to perform programmable tasks. Before describing our results in mor ...
... We believe theoretical techniques and algorithmic analysis can offer significant insight for the development of these machines. Our goal in this paper is to take one class of molecular machines and formally show how they can be used to perform programmable tasks. Before describing our results in mor ...
Biology EOC Review Questions
... Variation within species was important to the development of Darwin’s theory of evolution. Which statement does individual variation help explain? ...
... Variation within species was important to the development of Darwin’s theory of evolution. Which statement does individual variation help explain? ...
Chapter 8 Power Point
... • Activity theory - theory of adjustment to aging that assumes older people are happier if they remain active in some way, such as volunteering or developing a hobby. • Cellular clock theory - based on the idea that cells only have so many times that they can reproduce; once that limit is reached, d ...
... • Activity theory - theory of adjustment to aging that assumes older people are happier if they remain active in some way, such as volunteering or developing a hobby. • Cellular clock theory - based on the idea that cells only have so many times that they can reproduce; once that limit is reached, d ...
Extrachromosomal DNA
Extrachromosomal DNA is any DNA that is found outside of the nucleus of a cell. It is also referred to as extranuclear DNA or cytoplasmic DNA. Most DNA in an individual genome is found in chromosomes but DNA found outside of the nucleus also serves important biological functions.In prokaryotes, nonviral extrachromosomal DNA is primarily found in plasmids whereas in eukaryotes extrachromosomal DNA is primarily found in organelles. Mitochondrial DNA is a main source of this extrachromosomal DNA in eukaryotes. Extrachromosomal DNA is often used in research of replication because it is easy to identify and isolate.Extrachromosomal DNA was found to be structurally different from nuclear DNA. Cytoplasmic DNA is less methylated than DNA found within the nucleus. It was also confirmed that the sequences of cytoplasmic DNA was different from nuclear DNA in the same organism, showing that cytoplasmic DNAs are not simply fragments of nuclear DNA.In addition to DNA found outside of the nucleus in cells, infection of viral genomes also provides an example of extrachromosomal DNA.