Gene quantification using real-time quantitative PCR
... DNA copy number measurements are important in determining the extent of genomic imbalance that underlies most malignancies. There are numerous techniques available for measuring DNA copy number in tumors; each method has specific advantages and disadvantages. Chromosomal CGH can detect imbalances ac ...
... DNA copy number measurements are important in determining the extent of genomic imbalance that underlies most malignancies. There are numerous techniques available for measuring DNA copy number in tumors; each method has specific advantages and disadvantages. Chromosomal CGH can detect imbalances ac ...
Parallel Genetic and Phenotypic Evolution of DNA Superhelicity in
... function has recently been shown to be under strong selection in Escherichia coli. During the evolution of 12 initially identical populations propagated in a defined environment for 20,000 generations, parallel increases in DNA supercoiling were observed in ten populations. The genetic changes assoc ...
... function has recently been shown to be under strong selection in Escherichia coli. During the evolution of 12 initially identical populations propagated in a defined environment for 20,000 generations, parallel increases in DNA supercoiling were observed in ten populations. The genetic changes assoc ...
video slide - Dublin City Schools Home
... A Closer Look: Obtaining the Gene of Interest • How can a researcher obtain DNA that encodes a particular gene of interest? • The “shotgun” approach is one way to synthesize a gene of interest. – Millions of recombinant plasmids containing different segments of foreign DNA are produced. – This coll ...
... A Closer Look: Obtaining the Gene of Interest • How can a researcher obtain DNA that encodes a particular gene of interest? • The “shotgun” approach is one way to synthesize a gene of interest. – Millions of recombinant plasmids containing different segments of foreign DNA are produced. – This coll ...
π, γ
... 1. Remove all circular singletons of Π and Γ. 2. Lemma 1 Close every {π, π}-path ({γ, γ}-path) into a cycle by adding a single new adjacency to Π* (Γ*). 3. Form a maximum set of 2-bracelets (only chains remaining). 4. Form a maximum set of even 2-chains by linking pairs of πpaths (γ-paths) having ...
... 1. Remove all circular singletons of Π and Γ. 2. Lemma 1 Close every {π, π}-path ({γ, γ}-path) into a cycle by adding a single new adjacency to Π* (Γ*). 3. Form a maximum set of 2-bracelets (only chains remaining). 4. Form a maximum set of even 2-chains by linking pairs of πpaths (γ-paths) having ...
No Slide Title
... • Use in screening: PCR based detection of populations with anomalies in cell-cycle regulators e.g. L.O.H. of p16, cyclin over-expression, amplification of a gene • Detection of relapse/minimal residual disease • Use in prognosis: e.g. tumors with high S phase fraction detected by flow cytometry hav ...
... • Use in screening: PCR based detection of populations with anomalies in cell-cycle regulators e.g. L.O.H. of p16, cyclin over-expression, amplification of a gene • Detection of relapse/minimal residual disease • Use in prognosis: e.g. tumors with high S phase fraction detected by flow cytometry hav ...
IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS)
... atypical EPEC. Fourty isolates (80%) of E. coli found do not have any one of the specific virulence genes, these isolates identified as non-EPEC (Figures 1, 2, 3, 4 and Table 3). It was shown that some of E. coli isolates were carriedthe bfpAgene approximately, 200bp which represent the non-specific ...
... atypical EPEC. Fourty isolates (80%) of E. coli found do not have any one of the specific virulence genes, these isolates identified as non-EPEC (Figures 1, 2, 3, 4 and Table 3). It was shown that some of E. coli isolates were carriedthe bfpAgene approximately, 200bp which represent the non-specific ...
File
... The phenomenon of transformation was first discovered in 1928 by Frederick Griffith. In his now famous experiment, he injected mice with living non-pathogenic cells of the bacterium Streptococcus pneumonia and with heat-killed pathogenic of the same bacterium. The results displayed the properties of ...
... The phenomenon of transformation was first discovered in 1928 by Frederick Griffith. In his now famous experiment, he injected mice with living non-pathogenic cells of the bacterium Streptococcus pneumonia and with heat-killed pathogenic of the same bacterium. The results displayed the properties of ...
Genetic variation within Cape stumpnose, Sparidae) Rhabdosargus holubi
... geographic localities along the South African coastline from St Lucia in the northeast to Klein River in the southwest. Juveniles were sampled in estuaries and adults were collected in the marine intertidal zone. Mitochondrial DNA control region fragments of 368 bp in length were obtained from a tot ...
... geographic localities along the South African coastline from St Lucia in the northeast to Klein River in the southwest. Juveniles were sampled in estuaries and adults were collected in the marine intertidal zone. Mitochondrial DNA control region fragments of 368 bp in length were obtained from a tot ...
Capstone project 2015 in pdf format
... Description: Replication of organelle genomes has to be regulated in relation to the replication of the nuclear genome for faithful passage to the daughter cells. Counter to the large (>120 kb) organelle DNA found in other photosynthetic organisms, the chloroplast genomes of many dinoflagellates are ...
... Description: Replication of organelle genomes has to be regulated in relation to the replication of the nuclear genome for faithful passage to the daughter cells. Counter to the large (>120 kb) organelle DNA found in other photosynthetic organisms, the chloroplast genomes of many dinoflagellates are ...
INFINITI CYP450 2C19+ Assay
... for the detection and genotyping of the CYP2C19 allelic variants in genomic deoxyribonucleic acid (DNA) obtained from whole blood samples. The INFINITI CYP450 2C19+ Assay is comprised of the BioFilmChip® Microarray and the Intellipac® Reagent Module. The BioFilmChip Microarray consists of a polyeste ...
... for the detection and genotyping of the CYP2C19 allelic variants in genomic deoxyribonucleic acid (DNA) obtained from whole blood samples. The INFINITI CYP450 2C19+ Assay is comprised of the BioFilmChip® Microarray and the Intellipac® Reagent Module. The BioFilmChip Microarray consists of a polyeste ...
Identification and characterisation of Bacillus subtilis as cellulase
... in the presence of glucose and nitrate, some anaerobic growth can occur (Claus ...
... in the presence of glucose and nitrate, some anaerobic growth can occur (Claus ...
Preimplantation genetic diagnosis: State of the ART 2011
... For many years, this method remained unchanged. It was only in the ESHRE PGD Consortium data collection for cycles performed in 2004 that the laser was used more often that acid Tyrodes (Harper et al. 2008). The other change was the introduction of Ca2? Mg2? free biopsy media that reduced the juncti ...
... For many years, this method remained unchanged. It was only in the ESHRE PGD Consortium data collection for cycles performed in 2004 that the laser was used more often that acid Tyrodes (Harper et al. 2008). The other change was the introduction of Ca2? Mg2? free biopsy media that reduced the juncti ...
A genetic linkage map for watermelon based on
... Introduction (PI) 296341 (C. lanatus var. citroides) showed resistance to races 0, 1, and 2 of Fusarium oxysporum f. sp. niveum (Martyn and Netzer, 1991). A test with race 2 for progenies of a self-pollinated plant of PI 296341 resulted in a segregation of about three susceptible plants to one resis ...
... Introduction (PI) 296341 (C. lanatus var. citroides) showed resistance to races 0, 1, and 2 of Fusarium oxysporum f. sp. niveum (Martyn and Netzer, 1991). A test with race 2 for progenies of a self-pollinated plant of PI 296341 resulted in a segregation of about three susceptible plants to one resis ...
Variation 3.3
... and her family are your patients. Just before her twins, Sondra and Jason, were born, Ms. Lindsey’s husband, also African-American, died in an automobile accident. His parents were physiologically normal, but he had a brother who died of sickle cell disease at the age of 19. Ms. Lindsey explains to ...
... and her family are your patients. Just before her twins, Sondra and Jason, were born, Ms. Lindsey’s husband, also African-American, died in an automobile accident. His parents were physiologically normal, but he had a brother who died of sickle cell disease at the age of 19. Ms. Lindsey explains to ...
DNA methylation profiling identifies epigenetic dysregulation in
... pregnancy in rats have been shown to result in development of T2D in offspring (Inoue et al, 2009) while chronic high-fat diet in fathers programs b-cell dysfunction in female rat offspring (Ng et al, 2010). In humans, a reduced birth weight together with an accelerated growth in infancy has been as ...
... pregnancy in rats have been shown to result in development of T2D in offspring (Inoue et al, 2009) while chronic high-fat diet in fathers programs b-cell dysfunction in female rat offspring (Ng et al, 2010). In humans, a reduced birth weight together with an accelerated growth in infancy has been as ...
Mitochondrial Transcript Processing and Restoration of Male Fertility
... this hypothesis; the 1.85 kb transcript is a primary (initiated) transcript and hence not the result of RNA processing ( Kennell and Pring 1989). Plants segregating for the Rf1, Rf8, or Rf* restorers accumulate novel T-urf13 transcripts. Genetic analyses of four rf1-m alleles established that rf1-m ...
... this hypothesis; the 1.85 kb transcript is a primary (initiated) transcript and hence not the result of RNA processing ( Kennell and Pring 1989). Plants segregating for the Rf1, Rf8, or Rf* restorers accumulate novel T-urf13 transcripts. Genetic analyses of four rf1-m alleles established that rf1-m ...
Regulation of Bacterial Conjugation in Microaerobiosis by
... 2002; Zahrl et al. 2006; Lau-Wong et al. 2008). Some host-encoded regulators may control conjugal transfer in most (perhaps all) F-like plasmids while others may be plasmid specific. For instance, F transfer undergoes a drastic decrease in stationary phase (Frost and Manchak 1998), a behavior that i ...
... 2002; Zahrl et al. 2006; Lau-Wong et al. 2008). Some host-encoded regulators may control conjugal transfer in most (perhaps all) F-like plasmids while others may be plasmid specific. For instance, F transfer undergoes a drastic decrease in stationary phase (Frost and Manchak 1998), a behavior that i ...
Specialized Transduction by Bacteriophage P22 in Salmonella typhimurium: Genetic and Physical Structure of the Transducing Genomes and the Prophage Attachment Site.
... we found that DNA molecules from P22pro-I and P22pro-3 each contain a substitution which adds length to the composite genome making the intracellular replicated genome too long to fit into a single phage particle. In this respect, and in many of their biological properties, the proline-transducing p ...
... we found that DNA molecules from P22pro-I and P22pro-3 each contain a substitution which adds length to the composite genome making the intracellular replicated genome too long to fit into a single phage particle. In this respect, and in many of their biological properties, the proline-transducing p ...
Characterization of sex chromosomes in rainbow trout and coho
... Pardo et al., 2000). Our FISH analysis with the 5S ribosomal DNA probe in diverse rainbow trout strains confirms results described by Morán et al. (1996). As expected, fluorescent signals are localized in the NOR bearing chromosome pair and in males with heteromorphic sex chromosomes on one subteloc ...
... Pardo et al., 2000). Our FISH analysis with the 5S ribosomal DNA probe in diverse rainbow trout strains confirms results described by Morán et al. (1996). As expected, fluorescent signals are localized in the NOR bearing chromosome pair and in males with heteromorphic sex chromosomes on one subteloc ...
Caffeine overrides S. pombe SM checkpoint
... analysis of these mutants has provided important information about the mechanisms of checkpoint control, and from these studies a picture of how checkpoints may work at the molecular level is beginning to emerge. Two of the most extensively characterised pathways are the S-M checkpoint, which preven ...
... analysis of these mutants has provided important information about the mechanisms of checkpoint control, and from these studies a picture of how checkpoints may work at the molecular level is beginning to emerge. Two of the most extensively characterised pathways are the S-M checkpoint, which preven ...
transposon
... is given the prefix IS, followed by a number that identifies the type. The IS elements are normal constituents of bacterial chromosomes and plasmids. To describe an insertion into a particular site, a double colon is used; so λ::IS1 describes an IS1 element inserted into phage lambda. The IS ele ...
... is given the prefix IS, followed by a number that identifies the type. The IS elements are normal constituents of bacterial chromosomes and plasmids. To describe an insertion into a particular site, a double colon is used; so λ::IS1 describes an IS1 element inserted into phage lambda. The IS ele ...
Jigsaw handout - the Biology Scholars Program Wiki
... binds to lac repressor and causes a conformation change that alters the structure of lac repressor so that it cannot bind to DNA. Therefore, in the presence of lactose, lac repressor does not bind to the operator and RNA polymerase can transcribe the genes in the lac operon. Case study (homework or ...
... binds to lac repressor and causes a conformation change that alters the structure of lac repressor so that it cannot bind to DNA. Therefore, in the presence of lactose, lac repressor does not bind to the operator and RNA polymerase can transcribe the genes in the lac operon. Case study (homework or ...
Pairing of homologous regions in the mouse genome is associated
... cluster are expressed from the maternal allele, while a long noncoding RNA expressed from the paternal allele covers the locus to create a repressive compartment [15–17]. The maternal allele carries a germline methylation mark in the locus control region (KvDMR1), and monoallelic expression of the n ...
... cluster are expressed from the maternal allele, while a long noncoding RNA expressed from the paternal allele covers the locus to create a repressive compartment [15–17]. The maternal allele carries a germline methylation mark in the locus control region (KvDMR1), and monoallelic expression of the n ...
Selection of Suitable Endogenous Reference Genes for Relative
... trace the amplification of a target DNA sequence by monitoring fluorescence emitted from specific double-stranded DNA binding dyes or the fluorophore-labeled during the process of reaction [3,12,19]. In addition, it is valuable for the detection of rearrangements between two transgenic plant lines. ...
... trace the amplification of a target DNA sequence by monitoring fluorescence emitted from specific double-stranded DNA binding dyes or the fluorophore-labeled during the process of reaction [3,12,19]. In addition, it is valuable for the detection of rearrangements between two transgenic plant lines. ...
Extrachromosomal DNA
Extrachromosomal DNA is any DNA that is found outside of the nucleus of a cell. It is also referred to as extranuclear DNA or cytoplasmic DNA. Most DNA in an individual genome is found in chromosomes but DNA found outside of the nucleus also serves important biological functions.In prokaryotes, nonviral extrachromosomal DNA is primarily found in plasmids whereas in eukaryotes extrachromosomal DNA is primarily found in organelles. Mitochondrial DNA is a main source of this extrachromosomal DNA in eukaryotes. Extrachromosomal DNA is often used in research of replication because it is easy to identify and isolate.Extrachromosomal DNA was found to be structurally different from nuclear DNA. Cytoplasmic DNA is less methylated than DNA found within the nucleus. It was also confirmed that the sequences of cytoplasmic DNA was different from nuclear DNA in the same organism, showing that cytoplasmic DNAs are not simply fragments of nuclear DNA.In addition to DNA found outside of the nucleus in cells, infection of viral genomes also provides an example of extrachromosomal DNA.