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Case Study Learning via Simulations of Molecular Biology Techniques
Case Study Learning via Simulations of Molecular Biology Techniques

... The disease is multifactorial and inheritance patterns are complex. Some forms of familial Alzheimer disease appear to be inherited as autosomal dominant traits, while others are recessive. Spontaneous Alzheimer disease also can occur in the absence of inherited factors. Mutations in at least four g ...
Reading frame
Reading frame

... • a series of codons in DNA/RNA that specify the amino acid sequence of the protein that the gene codes for • begins with an initiation codon - usually (but not always) ATG • ends with a termination codon: TAA, TAG or TGA ...
Study Guide
Study Guide

... trisomic; those that have received just one copy of a chromosome are said to be monosomic for the chromosome.  Fig 15.12 shows non-disjunction. This leads to trisomy of which the most common example is Down’s syndrome (an aneuploid condition-chromosome 21). You should understand how this happens.  ...
Document
Document

...  Genetic complexity grows with structural complexity: eubacteria have ~103 genes, single-cell eukaryotes 104-105.  All organisms possess a lot of non-coding, or junk, DNA: stretches of nucleic acid that does not lead to proteins.  Useful to evolutionary biologists: • Mutations in junk DNA do not ...
State v. Johnson
State v. Johnson

... From a statistical standpoint, the situation is analogous to estimating the proportion of blond, blue-eyed, fair-skinned people in Europe by separately counting the frequencies of people with blond hair, people with blue eyes, and people with fair skin and calculating their proportions [by applicat ...
09_01.jpg
09_01.jpg

... Human Genome Project -- “Why sequence junk?!” • 90% of human genome (3.3x109) in finished status, ie 99% of euchromatin. • 45% of the genome are repeat sequences. • 5% of the genome encodes genes (1.5% is coding). • 35,000 ~ 40,000 genes with multiple splicing products per gene (build 34). • Finish ...
Day 1 General information • Lecture powerpoints under resources
Day 1 General information • Lecture powerpoints under resources

... regulation of the eukaryotic cell division cycle and have also been implicated in the control of gene transcription and other processes P54 RNA helicase: transcription factor, protein that binds to a specific DNA sequence to control the transcription of DNA to mRNA If the cell aborts the replication ...
Ledbetter Presentation 8/15/05
Ledbetter Presentation 8/15/05

Practical class № 1 (1)
Practical class № 1 (1)

... found out which consists of two cylinders and formed microtubules and located perpendicularly. There was determines that this organelle is a constituent of mitotical spindle of division in animal cells. The name of this organelle is: A. Mitochondria B. Rybosome C. ER D. Centrosome E. Lysosome 19. Th ...
rflp analysis of mitochondrial dna in the genus secale
rflp analysis of mitochondrial dna in the genus secale

... EcoRI, HaeIII, HindIII, MspI, PstI, SalI and XhoI, from which nine mitochondrial gene probes (atp6, atp9, atp1, cox1, nad3, nad6, nad9, pol-r, orf25) were hybridized, by means of digestion products, for seven species of the genus Secale. RFLP EcoRI/pol-r specific markers were determined for all the ...
Caenibacterium thermophilum is a later synonym of Schlegelella
Caenibacterium thermophilum is a later synonym of Schlegelella

... Fig. 1. Neighbour-joining tree, based on 16S rRNA gene sequences, showing the estimated phylogenetic relationships among S. thermodepolymerans, Caenibacterium thermophilum and the nearest members of the b-Proteobacteria. Accession numbers are given in parentheses. Bootstrap values are shown for rele ...
Package `rDNA`
Package `rDNA`

... This argument is only used if algorithm="attenuation" is set. When using the attenuation algorithm, ignore.agreement=TRUE specifies that the agreement variable should be ignored completely. For example, if the initial statement is positive and another actor uses the same concept in a negative way, a ...
presentation slides - Environmental Health and Safety
presentation slides - Environmental Health and Safety

... The deliberate transfer of a drug resistance trait to microorganisms that are not known to acquire the trait naturally, if such acquisition could compromise the ability to control disease agents in humans, veterinary medicine, or agriculture... Consideration should be given as to whether the drug re ...
Genetic Mapping with CAPS Markers
Genetic Mapping with CAPS Markers

... progeny to evaluate the number of crossing-over events between different regions of the chromosome and the gene AGO1 and thus to locate the gene. The F2 plants that are homozygous for the mutation of interest (+/+), and thus showing the mutant phenotype, will be used for mapping. Since both of their ...
2. If 20% of the DNA in a guinea pig cell is adenine, what
2. If 20% of the DNA in a guinea pig cell is adenine, what

genetics chapter - UBC Let`s Talk Science
genetics chapter - UBC Let`s Talk Science

... Remember how the plasmids carry cool genes that give antibiotic resistance to bacteria? Bacteria WITH plasmids will be able to live and grow on the Petri dish with antibiotics. Bacteria WITHOUT plasmids will die. So we know that all the bacteria that are living, growing and duplicating contain the p ...
Biotechnology Australia
Biotechnology Australia

... These antibodies remain in the body and protect against future infection by the naturally-occurring form of the disease. Vaccines have been and are still used to control a number of life-threatening diseases including measles, polio, tuberculosis and tetanus. However today the vaccines are developed ...
printer-friendly version of benchmark
printer-friendly version of benchmark

... enzyme called RNA polymerase opens the necessary gene in the DNA and begins adding complimentary nucleotides to “copy” the gene base sequence. RNA does not contain thymine; instead, it contains uracil (U). Therefore, as mRNA copies the gene from DNA, it pairs adenine with uracil, thymine with adenin ...
The Structure of the Human AGT Protein Bound to DNA
The Structure of the Human AGT Protein Bound to DNA

... lesions. Flipping out each base sequentially may not be the most efficient method. Our previous experiments indicate that a mispaired aberrant base in the helical structure is more likely to be detected by AGT than the same base in a Watson–Crick basepair.43 We suggest that finding weakened base-pai ...
Chapter 26 Presentation-Phylogeny and the Tree of Life
Chapter 26 Presentation-Phylogeny and the Tree of Life

... Different genes often evolve at different rates, even within the same evolutionary lineage. Molecular trees have the ability to encompass short and long periods of time because genes evolve at different rates. ...
Full Text - Harvard University
Full Text - Harvard University

... genomic sequence than exons. But what was the function of this prevalent genomic feature? Since introns are removed from the nascent RNA, they marked a departure from early studies of genetic code. Introns did not fit easily into the simple, linear transfer of genetic infor­ma­ tion from DNA to mRNA ...
Genetic Engineering via Bacterial Transformation
Genetic Engineering via Bacterial Transformation

... To SEE the Central Dogma in action: ...
Chapter 3: Presentation Slides
Chapter 3: Presentation Slides

... • Meiosis produces four cells, each of which contains one copy of each pair of homologous chromosomes = genetically haploid • Mitosis produces two cells which contain both members of each pair of homologous chromosomes = genetically diploid ...
DNA RNA Protein
DNA RNA Protein

... • DNA can only be synthesized from 5’ to 3’, by adding new nucleotides to the 3’ end. • This is a problem, because both strands must be synthesized at the replication fork, and one strand will necessarily be synthesized in the opposite direction from the movement of the replication fork. • In realit ...
Bacterial Genetics
Bacterial Genetics

... c. RNA polymerase binds to promoter region in a particular way and switches the genes on and transcripts are generated until it reaches termination sequence i. The termination sequence is a particular sequence that has been recognized by RNA polymerase and tells the RNA polymerase to stop. This is t ...
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Extrachromosomal DNA



Extrachromosomal DNA is any DNA that is found outside of the nucleus of a cell. It is also referred to as extranuclear DNA or cytoplasmic DNA. Most DNA in an individual genome is found in chromosomes but DNA found outside of the nucleus also serves important biological functions.In prokaryotes, nonviral extrachromosomal DNA is primarily found in plasmids whereas in eukaryotes extrachromosomal DNA is primarily found in organelles. Mitochondrial DNA is a main source of this extrachromosomal DNA in eukaryotes. Extrachromosomal DNA is often used in research of replication because it is easy to identify and isolate.Extrachromosomal DNA was found to be structurally different from nuclear DNA. Cytoplasmic DNA is less methylated than DNA found within the nucleus. It was also confirmed that the sequences of cytoplasmic DNA was different from nuclear DNA in the same organism, showing that cytoplasmic DNAs are not simply fragments of nuclear DNA.In addition to DNA found outside of the nucleus in cells, infection of viral genomes also provides an example of extrachromosomal DNA.
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