Fellows seminar 9-19-2014
... Is there support for epigenetics? Methylation is one of the commonly studied epigenetic marks addition of a methyl group to the 5-carbon position of a cytosine residue located next to guanine 1. methylation can lead to the binding of methylated CpG binding proteins and transcriptional repressor ...
... Is there support for epigenetics? Methylation is one of the commonly studied epigenetic marks addition of a methyl group to the 5-carbon position of a cytosine residue located next to guanine 1. methylation can lead to the binding of methylated CpG binding proteins and transcriptional repressor ...
Restriction Enzymes, Gel Electrophoresis and Mapping DNA
... • Ultracentrifugation—density uniform (except satellite DNA) • Hybridization kinetics—complexity of regions of DNA, no specifics ...
... • Ultracentrifugation—density uniform (except satellite DNA) • Hybridization kinetics—complexity of regions of DNA, no specifics ...
CR75th Anniversary Commentary
... portfolio of cellular machinery to implement these processes continues to unravel in what we now investigate every day as activation of, and heritably transmitting of, information from cell signaling pathways. These include switches in patterns of gene expression and the cell nuclear events that fix ...
... portfolio of cellular machinery to implement these processes continues to unravel in what we now investigate every day as activation of, and heritably transmitting of, information from cell signaling pathways. These include switches in patterns of gene expression and the cell nuclear events that fix ...
Genetics and Epigenetics of Human Disease
... 0.1% that we don’t have in common with everyone else. This is understandable given the excitement surrounding the Human Genome Project and the ubiquitous use of the DNA double helix icon in all things biomedical. But DNA alone is not destiny. Human development from conception to adulthood is an inse ...
... 0.1% that we don’t have in common with everyone else. This is understandable given the excitement surrounding the Human Genome Project and the ubiquitous use of the DNA double helix icon in all things biomedical. But DNA alone is not destiny. Human development from conception to adulthood is an inse ...
word
... Conjugation - Reproduction or "mating" of bacterial cells Example of how small amounts of recombinant DNA are “manufactured” through use of plasmids A. Plasmids carrying recombinant DNA can be used to infect bacteria such as E. coli B. These plasmids contain a small, circular piece of DNA ...
... Conjugation - Reproduction or "mating" of bacterial cells Example of how small amounts of recombinant DNA are “manufactured” through use of plasmids A. Plasmids carrying recombinant DNA can be used to infect bacteria such as E. coli B. These plasmids contain a small, circular piece of DNA ...
plasmid to transform
... • Taq polymerase is extracted from bacteria that live in hot springs, so they remain active at temperatures up to 90°C. ...
... • Taq polymerase is extracted from bacteria that live in hot springs, so they remain active at temperatures up to 90°C. ...
Unit 1 content check list
... Describe how many proteins can come from one gene Describe how post-translational modification can increase the number of forms of proteins Describe how amino acid code is folded into a protein ...
... Describe how many proteins can come from one gene Describe how post-translational modification can increase the number of forms of proteins Describe how amino acid code is folded into a protein ...
04/20
... 4. Telomeric and centromeric regions Features of Facultative Heterochromatin 1. Referred to as silent chromatin 2. Potential to become heterochromatic (Barr body) ...
... 4. Telomeric and centromeric regions Features of Facultative Heterochromatin 1. Referred to as silent chromatin 2. Potential to become heterochromatic (Barr body) ...
DNA Structure, Function and Replication 1
... By Dr. Ingrid Waldron, Department of Biology, University of Pennsylvania, © 2014 ; Teachers are encouraged to copy this Student Handout for classroom use. A Word file (which can be used to prepare a modified version if desired) and Teacher Preparation Notes with learning goals, instructional suggest ...
... By Dr. Ingrid Waldron, Department of Biology, University of Pennsylvania, © 2014 ; Teachers are encouraged to copy this Student Handout for classroom use. A Word file (which can be used to prepare a modified version if desired) and Teacher Preparation Notes with learning goals, instructional suggest ...
DNA extraction from cheek cells protocol I mailed to you
... By Dr. Ingrid Waldron, Department of Biology, University of Pennsylvania, © 2014 ; Teachers are encouraged to copy this Student Handout for classroom use. A Word file (which can be used to prepare a modified version if desired) and Teacher Preparation Notes with learning goals, instructional suggest ...
... By Dr. Ingrid Waldron, Department of Biology, University of Pennsylvania, © 2014 ; Teachers are encouraged to copy this Student Handout for classroom use. A Word file (which can be used to prepare a modified version if desired) and Teacher Preparation Notes with learning goals, instructional suggest ...
Genetics - PCB 3063
... • Enzymes that remove acetyl groups from histones are recruited to methylated DNA. – There are additional types of histone modification as well, such as methylation of the histones. ...
... • Enzymes that remove acetyl groups from histones are recruited to methylated DNA. – There are additional types of histone modification as well, such as methylation of the histones. ...
3. Mapping Epigenetic Seed Genes to Affymatrix
... If a “ESGi-LPj” pair was significantly straight similar, there must be a group of genes coxpressed with seed genes (ESG) whose up-regulated genes in LPj were enriched with the coexpressed genes with ESGi, and (or) whose down-regulated genes in LPj were enriched with the anti-coexpressed genes with E ...
... If a “ESGi-LPj” pair was significantly straight similar, there must be a group of genes coxpressed with seed genes (ESG) whose up-regulated genes in LPj were enriched with the coexpressed genes with ESGi, and (or) whose down-regulated genes in LPj were enriched with the anti-coexpressed genes with E ...
Biochemistry ± DNA Chemistry and Analysis DNA o Adenosine
... x Stacked bases in dsDNA shield UV absorption x Melting causes UV to increase absorbance by bases ± monitor melting based on absorbance in soln Heating causes H-bonds to disrupt, 2 strands separate x A=T melts at lower temps than GŁ& Annealing: H-bond formation allows dsDNA to form complementary ...
... x Stacked bases in dsDNA shield UV absorption x Melting causes UV to increase absorbance by bases ± monitor melting based on absorbance in soln Heating causes H-bonds to disrupt, 2 strands separate x A=T melts at lower temps than GŁ& Annealing: H-bond formation allows dsDNA to form complementary ...
This would be given at the end of the unit
... b. Extract the protein from the bacterial culture. c. Use a restriction enzyme to cut out the gene from human DNA. d. Transform bacteria with the recombinant plasmid. 4. Analyzing DNA by gel electrophoresis allows researchers to a. compare DNA samples from different sources. b. determine whether a p ...
... b. Extract the protein from the bacterial culture. c. Use a restriction enzyme to cut out the gene from human DNA. d. Transform bacteria with the recombinant plasmid. 4. Analyzing DNA by gel electrophoresis allows researchers to a. compare DNA samples from different sources. b. determine whether a p ...
The amount of DNA, # of genes and DNA per gene in various
... The first proof was provided In 1961 by measuring the ratio of different dinucleotides in DNA. The concentration of 5’AG3’ was equal to 5’CT3’ (as expected from an antiparallel orientation) and not equal to 5’TC3’ (as expected from a a parallel orientation). DNA sequencing in 1970s confirmed this co ...
... The first proof was provided In 1961 by measuring the ratio of different dinucleotides in DNA. The concentration of 5’AG3’ was equal to 5’CT3’ (as expected from an antiparallel orientation) and not equal to 5’TC3’ (as expected from a a parallel orientation). DNA sequencing in 1970s confirmed this co ...
Chapter 6 Genes and Gene Technology Section 1 We now know
... If any of the changes occur, a mutation results. The mutation may not have any affect on the organism, or it could cause harm to the organism to the point that death results. Mutations do happen, but we are very fortunate that many of these mistakes are repaired in the cell, but sometimes the mistak ...
... If any of the changes occur, a mutation results. The mutation may not have any affect on the organism, or it could cause harm to the organism to the point that death results. Mutations do happen, but we are very fortunate that many of these mistakes are repaired in the cell, but sometimes the mistak ...
File
... 2. describe the importance of DNA barcoding by listing several applications. 3. describe the goal of the iBOL project. 4. explain eBOL and navigate the website 5. explain the BOLD 6. give an outline of the process necessary for DNA barcoding of rockfish 7. explain the difference between nuclear and ...
... 2. describe the importance of DNA barcoding by listing several applications. 3. describe the goal of the iBOL project. 4. explain eBOL and navigate the website 5. explain the BOLD 6. give an outline of the process necessary for DNA barcoding of rockfish 7. explain the difference between nuclear and ...
learning_goals_objectives
... 4. draw a model to show the basic structure on a nucleotide 5. explain the bonds between consecutive nucleotides and the bonds between the bases, and explain their relative strengths. 6. state the central Dogma of Molecular Biology and understand when transcription and translation occur 7. generate ...
... 4. draw a model to show the basic structure on a nucleotide 5. explain the bonds between consecutive nucleotides and the bonds between the bases, and explain their relative strengths. 6. state the central Dogma of Molecular Biology and understand when transcription and translation occur 7. generate ...
Three epigenetic information channels and their different roles in
... emergence of complex multicellular organisms. Other authors have observed that in multicellular organisms, epigenetic marks will only be transmitted between generations provided they do not interfere with somatic differentiation and cell heredity (Jablonka & Lamb, 2005, pp. 148–150). Our contributio ...
... emergence of complex multicellular organisms. Other authors have observed that in multicellular organisms, epigenetic marks will only be transmitted between generations provided they do not interfere with somatic differentiation and cell heredity (Jablonka & Lamb, 2005, pp. 148–150). Our contributio ...
Name: “Berry Full of DNA” DNA Extraction Lab Question: What
... 6. When the test tube is about 1/8 full, remove the funnel. Discard any extra mashed specimen pulp with the cheesecloth. 7. MRS. C will do this for you! Slowly drizzle cold ethanol along the side of the test tube, until the test tube is about half full of liquid. The ethanol should form a separate l ...
... 6. When the test tube is about 1/8 full, remove the funnel. Discard any extra mashed specimen pulp with the cheesecloth. 7. MRS. C will do this for you! Slowly drizzle cold ethanol along the side of the test tube, until the test tube is about half full of liquid. The ethanol should form a separate l ...
chapter11
... Telomeric DNA can be lengthened by a DNA replicating enzyme called telomerase. Cells that produce telomerase continue to divide indefinitely beyond the point at which cell division would normally cease. Active telomerase is found in germ cells that give rise to sperm and eggs in animals, but it is a ...
... Telomeric DNA can be lengthened by a DNA replicating enzyme called telomerase. Cells that produce telomerase continue to divide indefinitely beyond the point at which cell division would normally cease. Active telomerase is found in germ cells that give rise to sperm and eggs in animals, but it is a ...
What is DNA Fingerprinting
... 2. Go to the following site: http://www.pbs.org/wgbh/nova/sheppard/analyze.html Before doing the activity, read the following background information: Background Essay: Create a DNA Fingerprint In the last 15 years, DNA has played an increasingly important role in our legal system. Tissue evidence is ...
... 2. Go to the following site: http://www.pbs.org/wgbh/nova/sheppard/analyze.html Before doing the activity, read the following background information: Background Essay: Create a DNA Fingerprint In the last 15 years, DNA has played an increasingly important role in our legal system. Tissue evidence is ...
Epigenetics
Epigenetics is the study, in the field of genetics, of cellular and physiological phenotypic trait variations that are caused by external or environmental factors that switch genes on and off and affect how cells read genes instead of being caused by changes in the DNA sequence. Hence, epigenetic research seeks to describe dynamic alterations in the transcriptional potential of a cell. These alterations may or may not be heritable, although the use of the term ""epigenetic"" to describe processes that are not heritable is controversial. Unlike genetics based on changes to the DNA sequence (the genotype), the changes in gene expression or cellular phenotype of epigenetics have other causes, thus use of the prefix epi- (Greek: επί- over, outside of, around).The term also refers to the changes themselves: functionally relevant changes to the genome that do not involve a change in the nucleotide sequence. Examples of mechanisms that produce such changes are DNA methylation and histone modification, each of which alters how genes are expressed without altering the underlying DNA sequence. Gene expression can be controlled through the action of repressor proteins that attach to silencer regions of the DNA. These epigenetic changes may last through cell divisions for the duration of the cell's life, and may also last for multiple generations even though they do not involve changes in the underlying DNA sequence of the organism; instead, non-genetic factors cause the organism's genes to behave (or ""express themselves"") differently.One example of an epigenetic change in eukaryotic biology is the process of cellular differentiation. During morphogenesis, totipotent stem cells become the various pluripotent cell lines of the embryo, which in turn become fully differentiated cells. In other words, as a single fertilized egg cell – the zygote – continues to divide, the resulting daughter cells change into all the different cell types in an organism, including neurons, muscle cells, epithelium, endothelium of blood vessels, etc., by activating some genes while inhibiting the expression of others.