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HONORS BIOLOGY FINAL EXAM STUDY GUIDE 2015
HONORS BIOLOGY FINAL EXAM STUDY GUIDE 2015

... 5. If an organism has 80 chromosomes, how would non-disjunction affect the resulting daughter cells during meiosis? Provide a specific example of a human genetic disorder resulting from non-disjunction. 6. Ms. Tung cuts her big toe. What cellular division process would be used to repair the damage? ...
Gene Cloning
Gene Cloning

... do not because the mixture contains a large excess of short DNA molecules, called oligonucleotides or primers, which anneal to the DNA molecules at specific positions. 3) The temperature is raised to 74˚C. This is the optimum working temperature for the Taq DNA polymerase that is present in the mixt ...
Dr. McKay`s lecture
Dr. McKay`s lecture

... ‘Essentially complete’ as of December 1998 Contains ~100 million bp on 6 chromosomes Predicted to contain ~20, 000 genes. ~ 55% of these genes are similar to genes from other organisms. • ~ 20% associated with mutationally defined genetic loci ...
Genetics Slides
Genetics Slides

... Offspring ...
Microbial Genetics
Microbial Genetics

... be internal complementary regions within an RNA strand that can form double-stranded “hairpin loops” (C to G; A to U) An RNA strand can also form a double-stranded structure with a DNA strand; in this case, the U on the RNA will base-pair with the A on the DNA. ...
Biology 321 Spring 2011 Answers to Assignment Set #5
Biology 321 Spring 2011 Answers to Assignment Set #5

... d. Examination of a normal control group is important because some sequence variations will be associated with disease and others will have no obvious effect on the encoded protein or on the organismal phenotype. In order to understand how the gene product functions and to assess the phenotypic impl ...
Methods
Methods

... familiarizing ourselves with the characteristics of bacteria in terms of how they grow, what they need to grow and so on. The first laboratory method that was taught was that of streaking for singles. To execute this procedure, a toothpick was dipped into a colony and used to inoculate the colony on ...
Structure of DNA and History
Structure of DNA and History

... “The central dogma deals with the detailed residue-by-residue transfer of sequential information. It states that such information cannot be transferred back from protein to either protein or nucleic acid.” ...
S x - IBIVU
S x - IBIVU

... 2 - Score or bit score is a value calculated from the number of gaps and substitutions associated with each aligned sequence. The higher the score, the more significant the alignment. Each score links to the corresponding pairwise alignment between query sequence and hit sequence (also referred to a ...
Advancements in Genetic Engineering
Advancements in Genetic Engineering

... components. Even functional compromise and defects in muscular components can be attributed to more than 30 genes and their intergenic sequences. Considering genes involved in nervous system that affect motor movement, the disease is subjected to more than one thousand causative DNA sequence variati ...
Chapter 25
Chapter 25

... substrates are ATP, GTP, CTP, and UTP, and PPi is one of products. Substrate hydrolysis provides synthesis energy. 3. After 10 nucleotides are added, σ-factor is dissociated from the enzyme. 4. RNA polymerase under- and over-winds DNA at the behind and ahead of transcription bubble, respectively. Th ...
Simple and straightforward construction of a mouse gene targeting
Simple and straightforward construction of a mouse gene targeting

... plasmid, pVgRXR (Invitrogen), by PCR using primer pair PGKEM7S and PGKEM7A (Table 1). The PCR product was annealed to a single-stranded DNA of pLox-neo prepared from an E.coli dut ung strain co-infected with M13 KO7 helper phage, and then transformed into a dut+ ung+ strain followed by selection f ...
Honors Biology Module 7 Cellular Reproduction
Honors Biology Module 7 Cellular Reproduction

... When a cell needs to make a protein, its DNA has the plan for making that protein in a long series of threenucleotide base sequences. Messenger RNA reads this sequence and makes a “negative image” of the relevant portion of DNA. It then takes this series of nucleotide base sequences out to the ribo ...
Mixed Questions
Mixed Questions

... 2. Describe how to experimentally determine mechanisms of DNA transfer ...
DustinHancks_proposal
DustinHancks_proposal

... Hybrid zones exist where the two species occur together in parts of Illinois and Missouri, in some cases with documentation of up to sixty years, yet they retain their specific identity (Braasch and Smith, 1965), (Thomerson, 1967). Mitochondrial DNA and microsatellite analysis has been performed rec ...
Chapter 4 genetics
Chapter 4 genetics

... • DNA wraps around proteins and compacts (made smaller) to be made into chromosomes. • Genes are on chromosomes • A gene is a segment of DNA at a specific location on a chromosome that influences heredity characteristic. ...
Genetic Techniques for Biological Research Chapter7
Genetic Techniques for Biological Research Chapter7

... RESEARCH ...
DNA
DNA

... • Genetic material can be introduced into single-celled organisms – it automatically passes through generations – difficult to do it in higher organisms. • The first method applied successfully to mammals was DNA microinjection (Gordon and Ruddle 1981). • In mammalian cells linear DNA fragments are ...
and the DNA
and the DNA

... • Chromosomes have unequal distribution of nucleotide sequences that are transcribe and translated • Our genome is smaller than we thought; only about 30,000 -40,000 genes • The same gene can encode different versions of a protein. An organism’s complete set of proteins is called its proteome. • Tra ...
Unit test review
Unit test review

...  Independent Work  Exit Question ...
STUDY OF VNTR HUMAN POLYMORPHISMS BY PCR
STUDY OF VNTR HUMAN POLYMORPHISMS BY PCR

... In all cases DNA segments are amplified and subsequently subjected to various analyzes and studies. In a PCR reaction, the first step is the preparation of the DNA sample that is extracted from various biological sources or tissues. In PCR, the DNA or gene to be amplified is defined as "target" and ...
Document
Document

... D. Testis ...
PowerPoint 簡報
PowerPoint 簡報

... In fact, it is unlikely that either Griffith or Avery had any real feeling for how important their work would ultimately become. Instead, each focused only on answering a very specific question. Griffith wondered, "Can bacteria change from one type to another?" and showed that they could. Avery ask ...
Comparison of p53 Structure: Wild type vs. mutant
Comparison of p53 Structure: Wild type vs. mutant

... • Notice that the portion of the protein that directly interacts with the DNA is highly conserved (purple) • Other protein regions are less highly conserved ...
8000 - International Commission on Missing Persons
8000 - International Commission on Missing Persons

... The total number of Srebrenica-related sites where human remains have been recoverd is 430. This includes 94 graves, and 336 surface sites. Over 17,000 sets of human remains (bodies and body parts) related to Srebrenica 1995 have been examined in mortuaries by pathologists and anthropologists. ...
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Genomics

Genomics is a discipline in genetics that applies recombinant DNA, DNA sequencing methods, and bioinformatics to sequence, assemble, and analyze the function and structure of genomes (the complete set of DNA within a single cell of an organism). Advances in genomics have triggered a revolution in discovery-based research to understand even the most complex biological systems such as the brain. The field includes efforts to determine the entire DNA sequence of organisms and fine-scale genetic mapping. The field also includes studies of intragenomic phenomena such as heterosis, epistasis, pleiotropy and other interactions between loci and alleles within the genome. In contrast, the investigation of the roles and functions of single genes is a primary focus of molecular biology or genetics and is a common topic of modern medical and biological research. Research of single genes does not fall into the definition of genomics unless the aim of this genetic, pathway, and functional information analysis is to elucidate its effect on, place in, and response to the entire genome's networks.
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