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Annexure `AAB-CD-01` L T P/S SW/FW TOTAL CREDIT UNITS 3 0 2
Annexure `AAB-CD-01` L T P/S SW/FW TOTAL CREDIT UNITS 3 0 2

... At the end of this course, the students will be able to:  Define and analyze the structural features of genetic materials  Describe the prokaryotic and eukaryotic gene expression  Describe mobile genetic elements  Define enzymes that are used to exploit cells and organisms Module I DNA & Protein ...
No Slide Title
No Slide Title

... 1) an enzymatic or other functional assay (specific DNA binding) 2) Western blotting if you have antibodies 3) recognizable band on an SDS-PAGE gel usually possible if the protein is over-expressed (but not so good if you are trying to purify functional protein) ...
THE IMMUNE SYSTEM
THE IMMUNE SYSTEM

... Viral DNA is created and inserts into cell’s DNA Infected cell divides with new DNA code Cell division creates raw protein material Raw infected material is packaged into an immature virus cell Leaves infected cell through “budding” New immature cell matures and then attacks another healthy cell ...
Dr, McKenna`s Slides
Dr, McKenna`s Slides

Document
Document

... •Homologues of mutS and mutL genes exist so enzymes involved in eukaryotic mismatch repair likely to be similar to prokaryotic enzymes. •BUT, no homologue of MutH (protein that recognizes unmethylated newly synthesized strand) so recognition of newly synthesized strand does not appear to occur via a ...
Biotechnology - Valhalla High School
Biotechnology - Valhalla High School

... What are restriction enzymes? • These enzymes were discovered in bacteria. • Each restriction enzyme recognizes a certain DNA sequence, and cuts it. • For example: A restriction enzyme may recognize the sequence, “TTGG”. • Everytime this “enzyme” sees this sequence, it cuts the strand between the T ...
lesson viii - MisterSyracuse.com
lesson viii - MisterSyracuse.com

... specific sequence of bases. It signals the start of a gene. 12. RNA polymerase attaches here, and starts adding bases, using the DNA as a template strand. It is much slower than DNA polymerase, at only 40 bases per second. 13. It moves along until it hits the terminator. “You have been targeted for ...
HIV and immunity
HIV and immunity

... This formidable array of defense mechanisms Allows HIV to avoid being suppressed by our immune system Integration and latency ...
Cell-mediated Immunity
Cell-mediated Immunity

... Listeria monocytogenes = Intracellular pathogen Experiment: Inject mouse with killed Listeria bacteria to stimulate immunity that enables mouse to resist challenge injection with live organisms Determine the mechanism of protection by transferring serum or cells from the immune mouse to a naïve reci ...
biocp_mar20
biocp_mar20

Unit 3 Biochemistry
Unit 3 Biochemistry

... 7. In DNA replication A pairs with ________, T pairs with __________, G pairs with ________, and C pairs with ________. 8. In transcription (DNA is used to make ____________). A pairs with ...
DNA Detectives What is Your DNA Alias? The central dogma of
DNA Detectives What is Your DNA Alias? The central dogma of

... We use four letters to code all the information contained in DNA: A, T, C and G. The letters are used in groups of three. A group is called a codon. DNA contains the information that is needed by your body to make proteins. The different proteins have specific functions, such as making our hearts, h ...
It`s in the genes – data storage turns to DNA
It`s in the genes – data storage turns to DNA

... ‘At the Institute, we share biological data with other scientists to improve their insights into life,’ said Goldman. ‘We add value to it and send it back into the research community via the Internet. But we realised that, as the volume of biological data we receive grows exponentially, our budget ...
Human Uteroglobin/SCGB1A1 Antibody
Human Uteroglobin/SCGB1A1 Antibody

... prolactin (1). Uteroglobin is found in blood, urine and other body fluids (1). Human Uteroglobin cDNA encodes a 21 amino acid (aa) signal sequence and a 70 aa  mature protein. It shares 53­56% aa identity with mouse, rat, bovine, canine, equine or rabbit Uteroglobin, and is active in mice (4). The m ...
Biochemistry ± DNA Chemistry and Analysis DNA o Adenosine
Biochemistry ± DNA Chemistry and Analysis DNA o Adenosine

... Genomic Library for isolating a specific gene ± has non-coding regions in it x cDNA is just the coding region and will have introns removed to generate protein x Vector: DNA to be inserted is small (6-10kb) o ȜSKDJHYHFWRUV can handle up to 20kb ± infects E.coli o COSmids: can handle up to 50kb ƒ P ...
Foundations in Microbiology
Foundations in Microbiology

... 1. Do not occur simultaneously – transcription occurs in the nucleus and translation occurs in the cytoplasm. 2. Eucaryotic start codon is AUG, but it does not use formyl-methionine. 3. Eucaryotic mRNA encodes a single protein, unlike bacterial mRNA which encodes many. 4. Eucaryotic DNA contains int ...
Chapter
Chapter

... which causes lysis of the target cells. C8 consists of three subunits C8A, C8B, and C8G. This study focuses on the porcine C8G gene (pC8G) aiming to identify its cDNA sequence, to detect single nucleotide polymorphisms (SNPs) within the gene, and to analyze its polymorphic association with hemolytic ...
RNA and Protein Synthesis
RNA and Protein Synthesis

... others are red blood cells that have lost their nuclei and are packed with hemoglobin? Why are cells so different in structure and function? If the characteristics of a cell depend upon the proteins that are synthesized, what does this tell you about protein synthesis? Work with a partner to discuss ...
Ch. 7 Gene Expresion part 2
Ch. 7 Gene Expresion part 2

... D A base-pair substitution in DNA replaces a thymine with an adenine. When the altered mRNA is translated, valine replaces glutamate as the sixth amino acid of the new polypeptide chain. Hemoglobin with this chain is called HbS, or sickle hemoglobin. ...
Unit 9 Classification and Microorganisms
Unit 9 Classification and Microorganisms

... right away. The virus remains inactive for a period of time. The viral DNA that is embedded into the host’s DNA is called a prophage. ...
Cryptography and Linguistics of Macromolecules Cryptography and
Cryptography and Linguistics of Macromolecules Cryptography and

... In general terms, an MSA process results in a set of aligned sequences, usally with a calculation of the relative similarity among them, and a model of the alignment, usually with some score of its reliability. This model conveys the recu rrencies found in the set of sequences, and can be expressed ...
Biology
Biology

... types not including the kidney.  B. The cord blood stem cells are multipotent and thus can differentiate only into limited  cell types not including the kidney.  C. The kidney contains many different types of stem cells which are only available at the  embryonic cell stage.  D. The kidney does not c ...
PPT
PPT

...  Based on DNA properties & WC complementarity  The inherent three dimensional structure of DNA & self-assembly  DNA tiles(double & triple cross-over molecules)  Branched junction, graph-like DNA structure - Splicing of tree like structure(junction & graph-like DNA) - these model are yet to be co ...
homologous recombination
homologous recombination

Biology 3 Questions  1. Which is found in prokaryotic cell? (Cell)
Biology 3 Questions 1. Which is found in prokaryotic cell? (Cell)

... a) B cells are lymphocytes that mature in bone marrow and mediate antibody directed immunity b) Stem cells in the bone marrow produce lymphocyte by meiosis c) Antibodies are produced by B cell and can bind to antigen d) In cell-mediated immunity T cell respond to virally infected cells. e) all true ...
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DNA vaccination



DNA vaccination is a technique for protecting an animal against disease by injecting it with genetically engineered DNA so cells directly produce an antigen, resulting in a protective immunological response. Several DNA vaccines have been released for veterinary use, and there has been promising research using the vaccines for viral, bacterial and parasitic diseases, as well as to several tumour types. Although only one DNA vaccine has been approved for human use, DNA vaccines may have a number of potential advantages over conventional vaccines, including the ability to induce a wider range of immune response types.
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