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DNA - Belle Vernon Area School District
DNA - Belle Vernon Area School District

... First case to use DNA typing – 1983. 15 year old girl was raped and murdered. No evidence. Three years latter another girl in Narborough was raped and murdered in a similar manner. 17 year old Rodney Buckland was arrested making a statement in the second murder but proclaiming his innocence in the e ...
Recombinant DNA Lab
Recombinant DNA Lab

... Recombinant DNA refers to DNA of one organism inserted into the DNA of another. A Transformation refers to the process of creating recombinant DNA. The major tools of recombinant DNA technology are bacterial enzymes called restriction enzymes. Each enzyme recognizes a short, specific nucleotide sequ ...
Immune system
Immune system

Innate Immune Response to Ebolavirus Infection
Innate Immune Response to Ebolavirus Infection

... rates are approximately 40 to 90%, depending on the virus, with ZEBOV and MARVAngola being the most virulent. There are no FDA-approved vaccines or therapeutics to combat EBOV or MARV infection, and we still lack an understanding of the host’s innate immune response to these Category A Priority Path ...
Chapter 8: Cell Division
Chapter 8: Cell Division

... 1. Understand the two-step process of protein synthesis (transcription and translation); also define what a protein is. 2. Know the difference between DNA and RNA. 3. Know the three different types of RNA. 4. Know what codons are and their role in determining the amino acid composition of proteins; ...
FANCE Antibody
FANCE Antibody

... (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersen ...
IMMU 7630 PRACTICE MIDTERM
IMMU 7630 PRACTICE MIDTERM

... C. IgM 8. Cells that express class II MHC on their surface include: A. B cells, Dendritic cells, Helper T cells B. B cells, Macrophages, Dendritic cells C. Cytotoxic T cells, Macrophages, Dendritic cells 9. When a T-independent antigen, such as a complex carbohydrate, is injected, you expect to see ...
Chapter 20
Chapter 20

... A genomic library that is made using bacteria is the collection of recombinant vector clones produced by cloning DNA fragments from an entire genome  Types of Libraries:  Bacteriophage: stored as a collection of phage clones  BAC: A bacterial artificial chromosome (BAC) is a large plasmid that ha ...
Adverse Immune Reactions and Immune Deficiencies
Adverse Immune Reactions and Immune Deficiencies

... Acquired immune deficiency syndrome (AIDS). The immune response becomes defective as a result of exposure to the Human Immunodeficiency Virus (HIV). The HIV infects selectively CD4+ T cells and macrophages. The disease is usually lethal due to loss of CD4+ T cells. B cell and T cell tumours (myeloma ...
View/Open - Repository Unhas
View/Open - Repository Unhas

... The purpose of this research was to apply a non-detrimental technique to screen shrimp broodstocks for virus infection. Individual or pooled feces samples were collected and analyzed using multiplex PCR. The total of 125 and 75 broodstocks from Bombana and Aceh, respectively, were examined for virus ...
DNA and Genetic Material
DNA and Genetic Material

... the T-region into plant nuclear genome • Insert gene of interest and marker in the T-region by restriction enzymes – the pathogen will then “infect” the plant material • Works fantastically well with all dicot plant species ...
Explain what genetic recombination is, why it is important and ho it
Explain what genetic recombination is, why it is important and ho it

... prepare a DNA vaccine vaccine,, a gene from a pathogen is artificially replicated and then injected directly into uninfected people. If human cells take up the gene, some may use it to make the protein encoded by the gene.  The presence of the foreign protein in the body triggers an immune response ...
Lecture 7
Lecture 7

... • pigmented lesions on areas of the skin exposed to the sun and • an elevated incidence of skin cancer. It turns out that XP can be caused by mutations in any one of several genes - all of which have roles to play in NER. James Cleaver went around and collected cells from hundreds of these patients. ...
Custom Polyclonal Antibody Order Information
Custom Polyclonal Antibody Order Information

... (In order to improve the success rate of the service, it is recommended to provide more than 5 mg protein antigen and the purity should be higher than 90%. Please provide relevant information as much as possible.) ...
Molecular Genetics And Otolaryngology
Molecular Genetics And Otolaryngology

... therapy: gene addition and gene replacement. In general gene addition is more practical, as it is the expression of a normal allele that is required. Gene replacement, in contrast, has never been accomplished in vivo. The goal of gene therapy is the improvement of a patient’s health by correction of ...
Z333 Lecture
Z333 Lecture

... All cells have MHC molecules – most body cells have MHC I (lymphocytes have MHC II in addition) MHC molecules displayed on cell surface – each binds a specific peptide foreign fragment then “displays” it on surface. ...
Composite Transposons
Composite Transposons

... 700 to 5000 bp which can move from one location in a DNA sequence to another. They have short 16-41 bp inverted repeats on their ends. They encode a transposase which catalyses site-specific recombination. ...
Infections
Infections

... Roseola (exanthem subitum): A 4-year-old child experiences a rapid onset of high fever that lasts for 3 days and then suddenly returns to normal. Two days later, a maculopapular rash appears on the trunk and spreads to other parts of the body. ...
Cancer Gene Detection
Cancer Gene Detection

... The p53 tumor suppressor protein The p53 gene like the Rb gene, is a tumor suppressor gene, i.e., its activity stops the formation of tumors. If a person inherits only one functional copy of the p53 gene from their parents, they are predisposed to cancer and usually develop several independent tumor ...
Alternative Approaches to Molecular Biology
Alternative Approaches to Molecular Biology

... Since each strand of the starting DNA is used as a template for one copy of the replicated DNA (semiconservative replication) one copy will be shorter than the other. After many, many rounds of replication, cells with ...
No Slide Title
No Slide Title

... Upstream Elements of Class II • Can be several of these, two that are often found: 1. GC boxes (GGGCGG and CCGCCCC) – Stimulate transcription in either orientation – May be multiple copies – Must be close to TATA box (different from enhancers) – Bind the Sp1 factor 2. CCAAT box – Stimulates transcr ...
Slide 1
Slide 1

... 12.6 Recombinant cells and organisms can mass-produce gene products  Recombinant cells and organisms constructed by DNA technologies are used to manufacture many useful products, chiefly proteins.  Bacteria are often the best organisms for manufacturing a protein product because bacteria – have p ...
Gene Therapy and Genetic Counseling
Gene Therapy and Genetic Counseling

... • Remove parts of virus that cause symptoms or disease • Also remove parts of virus that trigger immune reaction • Add corrected gene to viral DNA • Administered this virus to specific cells that need functional protein • Choose “best” virus for the job ...
Supplemental Materials and Methods (doc 44K)
Supplemental Materials and Methods (doc 44K)

... The sampling area is located in the Russian discontinuous permafrost zone (62°57'E, 67°03'N). The sampling area has a mean annual temperature of -5.8°C and a mean annual precipitation of 505 mm. Cryoturbated peat circles (4 to 25 m in diameter) which develop through soil mixing as a result of frost ...
Section 13-2
Section 13-2

... some interesting things. Scientists have developed many transgenic organisms, which are organisms that contain genes from other organisms. Recently, scientists have removed a gene for green fluorescent protein from a jellyfish and tried to insert it into a monkey. ...
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DNA vaccination



DNA vaccination is a technique for protecting an animal against disease by injecting it with genetically engineered DNA so cells directly produce an antigen, resulting in a protective immunological response. Several DNA vaccines have been released for veterinary use, and there has been promising research using the vaccines for viral, bacterial and parasitic diseases, as well as to several tumour types. Although only one DNA vaccine has been approved for human use, DNA vaccines may have a number of potential advantages over conventional vaccines, including the ability to induce a wider range of immune response types.
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