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Chloramphenicol PDF
Chloramphenicol PDF

... Chloramphenicol inhibits host protein synthesis and thus prevents replication of the host chromosome. Plasmid replication, however, is independent of newly synthesized proteins and continues for several hours until up to 2000–3000 copies per cell are accumulated. Alternatively, the cell culture can ...
DNA Structure: Gumdrop Modeling Student Version
DNA Structure: Gumdrop Modeling Student Version

... identify what is different between the DNA of the plant, mammal, and bacterium. Compare the plant and mammal DNA. ...
Ataxia- telangiectasia Mutated (ATM)
Ataxia- telangiectasia Mutated (ATM)

... DNA can be damaged from two sources: ...
9700 BIOLOGY
9700 BIOLOGY

... 4 more than one strain (needs more than one type of vaccine) ; A more than one type (that causes cholera) R constantly mutating 5 vaccine, only gives short-term protection / requiring boosters ; 6 antigenic concealment ; 7 qualified ; e.g. organism in intestines, difficult for antibodies to reach 8 ...
Circulation and gas exchange
Circulation and gas exchange

... Lymphocytes originate from pluripotent stem cells in bone marrow, or in the fetus, mainly in liver. Lymphocytes are alike; they differentiate into T cells or B cells, depending on where they continue their maturation (Fig. 39.6) p858 Lymphocytes that migrate from the bone marrow to the thymus, a gla ...
Overview of B-Cell Development
Overview of B-Cell Development

... • The receptors produced by each lymphocyte have a unique antigen specificity which is determined by the structure of their antigen-binding site. • The wide range of antigen specificities in the antigen receptor repertoire is due to variation in the amino acid sequence in the V region. • Each indivi ...
Genetics exam 4
Genetics exam 4

... C. Involves proof-reading of the mRNA D. Prokaryotic RNA usually undergoes nuclear processing E. Polypeptides are synthesized by addition of amino acids to the amino terminus _____ Which of the following features is common to both DNA replication and transcription? A. Nucleotides are added to the 5' ...
Biotech & Genetic Engineering PP
Biotech & Genetic Engineering PP

... A transgenic organism has one or more genes from another organism inserted into the genome. ...
8 Expression and Modification of Recombinant Proteins
8 Expression and Modification of Recombinant Proteins

... Gene initially cloned and plasmid propagated in bacterial cells Mammalian cells transformed by electroporation (with linear plasmid) and gene integrates (1 or more times) into random locations within different CHO chromosomes Multiple rounds of growth and selection using methotrexate to select for t ...
Hepatitis Viruses
Hepatitis Viruses

... The leading cause of cirrhosis, a common cause of hepatocellular carcinoma The leading reason for liver transplantation. Coinfection with HIV is common. Prevalence is higher in some countries in Africa and Asia. Egypt has the highest serovalence for HCV (20% in some areas). ...
5.4 PPT_Codon Charts
5.4 PPT_Codon Charts

... (mRNA) to bring to the necklace factory (ribosomes).  The Delivery Trucks (tRNA) bring the ingredients (amino acids) to the ...
Polymerase Chain Reaction (PCR) + Electrophoresis with agarose
Polymerase Chain Reaction (PCR) + Electrophoresis with agarose

... separation of the DNA fragments from your PCR reaction is accomplished by exploiting the mobilities with which different sized molecules are able to traverse the gel. Longer molecules migrate more slowly because they experience more drag within the gel. Because the size of the molecule affects its m ...
CureVac`s Innovative Approach for Prophylactic Vaccination
CureVac`s Innovative Approach for Prophylactic Vaccination

... mRNA is a very promising molecule for prophylactic vaccines. Its physiological role is to transfer genetic, protein-building information from the cell’s nucleus to the cytoplasm where this information is translated into the corresponding protein. CureVac embraces this natural mechanism in order to e ...
Biology 120 Lab Exam 2 Review Session
Biology 120 Lab Exam 2 Review Session

Cell Type
Cell Type

... their antibody class from IgM to IgG, IgA, and sometimes IgE (Link to page 15 – Antibody classes and link from page 15 back to page 9). Changes in antibody class are directed by cytokines produced by T-cells. Memory cells do not take part in the initial direct battle – they persist, travelling the n ...
Genes: Structure, Replication, & Mutation
Genes: Structure, Replication, & Mutation

... spontaneously due to error during DNA replication Induced mutation: one that has been caused by damage resulting from chemical or radiation treatment (mutagen) ...
Biology Standard 2 Test Prep
Biology Standard 2 Test Prep

... A. The number of chromosomes increases from haploid to diploid. B. The number of chromosomes decreases from diploid to haploid. C. There is a segregation of dominant and recessive genes. D. There is an integration of dominant and recessive genes. 16. Which is true of meiosis? A. Identical cells are ...
Ch 14 Lymphatic System
Ch 14 Lymphatic System

... destroy the transplanted tissue -similar to cellular immune response against non-self antigens -The greater the difference between cell surface molecules the quicker and severe the reaction -Matching up donor and recipient tissues better prevents severe reactions -Immunosuppressive drugs are given t ...
Gene Therapy - Problems And Challenges
Gene Therapy - Problems And Challenges

... expression are the crucial issues for clinically relevant gene therapy. • Viruses are naturally evolved vehicles which efficiently transfer their genes into host cells. This ability made them desirable for engineering virus vector systems for the delivery of therapeutic genes. • The viral vectors re ...
BIOL212ImmuneSystemNotes
BIOL212ImmuneSystemNotes

... molecules MHC (major histocompatibility complex) molecules are host proteins that display the antigen fragments on the cell surface In infected cells, MHC molecules bind and transport antigen fragments to the cell surface, a process called antigen presentation A T cell can then bind both the antigen ...
Exam #3 Review
Exam #3 Review

... falls off the mRNA and dissociates into its subunits. CAUTION: Often it is easy to feel comfortable with the principles and processes of replication, transcription and translation. However, I find that it is more difficult when trying to answer questions that ask one to relate the three: Practice: W ...
Supplemental Note
Supplemental Note

... Genes were annotated with functional data from the Gene Ontology (GO) consortium (http://www.geneontology.org). We considered GO terms that were annotated at Level 3 or greater and were represented by at least 10 but not more than 1000 genes. A GO term was considered to be significantly changed by ...
Anti-food polysaccharide antibody formation in Inflammatory
Anti-food polysaccharide antibody formation in Inflammatory

... high level of antibodies against yeast polysaccharide such as ß-glucans. These antibodies may be a factor triggering gut inflammation through over-activation of the gut immune system in IBD patients. In this project, we hypothesise IBD patients develop antibodies against other food polysaccharides ( ...
The Mediators of Inflammation.
The Mediators of Inflammation.

... • GCs, IL10 and some other factors can inhibit iNOS or its induction. • With active oxygen, NO can form peroxynitrite which is a potent cytotoxic agent. • Can be blocked in (e.g.septic shock) by arginine analogues such as L-NMMA. • NO is scavenged by haemoglobin and reacts with thiols. ...
2421_Ch17.ppt
2421_Ch17.ppt

... the protein fractions are called globulins (fig. 17.2) most antibodies are found in the gamma fraction, thus this antibodyrich serum component is called immune serum globulin or gamma globulin ...
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DNA vaccination



DNA vaccination is a technique for protecting an animal against disease by injecting it with genetically engineered DNA so cells directly produce an antigen, resulting in a protective immunological response. Several DNA vaccines have been released for veterinary use, and there has been promising research using the vaccines for viral, bacterial and parasitic diseases, as well as to several tumour types. Although only one DNA vaccine has been approved for human use, DNA vaccines may have a number of potential advantages over conventional vaccines, including the ability to induce a wider range of immune response types.
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