First report of a tetracycline-inducible gene
... OriC, S. citri chromosomal replication origin; ori, colE1 replication origin from pBS+; Ampr and Gmr, genes conferring resistance to ampicillin and gentamicin, respectively. Tnp, transposase gene of Tn4001; TetR, tetracycline repressor gene from E. coli; Spi, S. citri spiralin gene devoid of its own ...
... OriC, S. citri chromosomal replication origin; ori, colE1 replication origin from pBS+; Ampr and Gmr, genes conferring resistance to ampicillin and gentamicin, respectively. Tnp, transposase gene of Tn4001; TetR, tetracycline repressor gene from E. coli; Spi, S. citri spiralin gene devoid of its own ...
Preferential X-chromosome inactivation, DNA
... of X-linked genes. The observed methylation differences may be summarised as follows (reviewed in Monk, 1986): (1) CpG islands at the 5' end of X-linked genes are methylated on the inactive X chromosome. In contrast, other CpG sequences in the body of X-linked genes may be more methylated on the act ...
... of X-linked genes. The observed methylation differences may be summarised as follows (reviewed in Monk, 1986): (1) CpG islands at the 5' end of X-linked genes are methylated on the inactive X chromosome. In contrast, other CpG sequences in the body of X-linked genes may be more methylated on the act ...
Cytokine responses in metal-induced allergic contact in Jacob Taku Minang
... potentially dangerous substances. These cells are thought to recognise particular highly conserved pathogen-associated molecular patterns or PAMP’s (e.g carbohydrate structures such as lypopolysaccharides; LPS) present on many different microorganisms (reviewed in Franc et al., 1999; Teixeira et al. ...
... potentially dangerous substances. These cells are thought to recognise particular highly conserved pathogen-associated molecular patterns or PAMP’s (e.g carbohydrate structures such as lypopolysaccharides; LPS) present on many different microorganisms (reviewed in Franc et al., 1999; Teixeira et al. ...
IMMUNOMODULATORY ACTIVITY OF ALCOHOLIC EXTRACT OF HABENARIA INTERMEDIA IN MICE Research Article
... Immunomodulatory agents that are free from side effects and which can be administered for long duration to obtain a continuous immune activation are highly desirable for the prevention of diseases. There a variety of naturally and chemically derived compound discovered with the Immunomodulatory acti ...
... Immunomodulatory agents that are free from side effects and which can be administered for long duration to obtain a continuous immune activation are highly desirable for the prevention of diseases. There a variety of naturally and chemically derived compound discovered with the Immunomodulatory acti ...
Restriction Fragment Length Polymorphism of hsp70
... Previous studies from our laboratory have demonstrated that the intermediate phenotype of thermosensitivity is present in hypertensive mice and rats. Increased expression of hsp70 caused by increased transcription rate was demonstrated in vivo, in organs, and in cultured cells from spontaneously hyp ...
... Previous studies from our laboratory have demonstrated that the intermediate phenotype of thermosensitivity is present in hypertensive mice and rats. Increased expression of hsp70 caused by increased transcription rate was demonstrated in vivo, in organs, and in cultured cells from spontaneously hyp ...
High Level Expression of HLA-A*0203
... mixing the biotinylated monomer with streptavidin-PE at a ratio of 4:1. Flow cytometry showed that this tetramer could specifically react with antigen-specific CD8+ T cells, indicating that it was biologically functional. These results provide a foundation for further characterization of antigen-spe ...
... mixing the biotinylated monomer with streptavidin-PE at a ratio of 4:1. Flow cytometry showed that this tetramer could specifically react with antigen-specific CD8+ T cells, indicating that it was biologically functional. These results provide a foundation for further characterization of antigen-spe ...
Detection of fg/mL Levels of GM
... differentiation of myeloid progenitor cells into immune cells. Chemotherapy patients are sometimes given recombinant GM-CSF (molgramostim or sargramostim) to increase production of white blood cells and prevent neutropenia. The basal levels of GM-CSF in blood are in the fg/mL range, and are therefor ...
... differentiation of myeloid progenitor cells into immune cells. Chemotherapy patients are sometimes given recombinant GM-CSF (molgramostim or sargramostim) to increase production of white blood cells and prevent neutropenia. The basal levels of GM-CSF in blood are in the fg/mL range, and are therefor ...
Characterisation of the immune response after - diss.fu
... apoptosis. Nevertheless, a significant number of cells persists and become memory cells after antigen elimination. A hallmark of protective immunity is determined by the clonally selection of antigenspecific B cells that generate neutralizing antibodies (Pulendran and Ahmed 2006). B lymphocytes, mar ...
... apoptosis. Nevertheless, a significant number of cells persists and become memory cells after antigen elimination. A hallmark of protective immunity is determined by the clonally selection of antigenspecific B cells that generate neutralizing antibodies (Pulendran and Ahmed 2006). B lymphocytes, mar ...
Homo-Dimeric Spherulin 3a: A Single
... presence of DTT as a reducing agent clearly proves this interpretation to be valid. To ensure a well-defined state of association of the native protein and to avoid side reactions of the cysteine residues (e.g., aggregation of the thermally denaturated protein), the cysteine in position 4 was replac ...
... presence of DTT as a reducing agent clearly proves this interpretation to be valid. To ensure a well-defined state of association of the native protein and to avoid side reactions of the cysteine residues (e.g., aggregation of the thermally denaturated protein), the cysteine in position 4 was replac ...
ISG15 Is Critical in the Control of Chikungunya Virus - HAL
... during an antiviral immune response has not been examined. The rapid induction of ISG15 following IFN stimulation has led to the identification of an antiviral role for ISG15 during infection. ISG152/2 mice display increased susceptibility to SINV, herpes simplex virus-1 (HSV-1), gamma herpes virus ...
... during an antiviral immune response has not been examined. The rapid induction of ISG15 following IFN stimulation has led to the identification of an antiviral role for ISG15 during infection. ISG152/2 mice display increased susceptibility to SINV, herpes simplex virus-1 (HSV-1), gamma herpes virus ...
Jigsaw handout - the Biology Scholars Program Wiki
... When lactose is present, it is taken into the cell and converted to allolactose. Allolactose binds to lac repressor and causes a conformation change that alters the structure of lac repressor so that it cannot bind to DNA. Therefore, in the presence of lactose, lac repressor does not bind to the ope ...
... When lactose is present, it is taken into the cell and converted to allolactose. Allolactose binds to lac repressor and causes a conformation change that alters the structure of lac repressor so that it cannot bind to DNA. Therefore, in the presence of lactose, lac repressor does not bind to the ope ...
Isolation and characterization of a repeated sequence (RPS1) of
... were virtually identical; however, one or two chromosomes were variable in size (Asakura et al., 1991). In virtually all cases, the chromosome that varied in size was chromosome 2. This suggested that chromosome 2 is too variable to be useful for distinguishing between strains. A similar variable ch ...
... were virtually identical; however, one or two chromosomes were variable in size (Asakura et al., 1991). In virtually all cases, the chromosome that varied in size was chromosome 2. This suggested that chromosome 2 is too variable to be useful for distinguishing between strains. A similar variable ch ...
Local Immune Responses in Human Tuberculosis: Learning From
... Importantly, the onset of adaptive immunity in human tuberculosis is delayed compared with other infections, which allows the bacterial load in the lung to expand significantly at the early stages of infection [3]. Studies from the site of tuberculosis infection in the murine lung have demonstrated ...
... Importantly, the onset of adaptive immunity in human tuberculosis is delayed compared with other infections, which allows the bacterial load in the lung to expand significantly at the early stages of infection [3]. Studies from the site of tuberculosis infection in the murine lung have demonstrated ...
Document
... Mendel’s Law of Segregation A. Law of Segregation states that a pair of factors (alleles) is segregated, or separated, during the formation of gametes (reproductive cells) (1) When two gametes combine during fertilization, the offspring have two factors controlling a specific trait (Gg) ...
... Mendel’s Law of Segregation A. Law of Segregation states that a pair of factors (alleles) is segregated, or separated, during the formation of gametes (reproductive cells) (1) When two gametes combine during fertilization, the offspring have two factors controlling a specific trait (Gg) ...
... were isolated with a tightly linked RAPD marker, and subsequently the regions flanking the Rps1 locus were nearly saturated with AFLP markers. Identification and mapping of RAPD markers linked to the Rps1 locus. The screening of 400 decamer primers against the cultivar Williams (rps1-k) and its NIL ...
CYP2B6 NESTED PCR: A GOOD APPROACH FOR PATIENTS ON METHADONE Original Article
... Methadone is a μ-receptor agonist valued for its effectiveness in the treatment of opioid dependency and the management of pain. It has high, 70-90%, oral bioavailability and a long plasma elimination half-life when administered in a racemic mixture [1–4], as shown by studies of differential blood-m ...
... Methadone is a μ-receptor agonist valued for its effectiveness in the treatment of opioid dependency and the management of pain. It has high, 70-90%, oral bioavailability and a long plasma elimination half-life when administered in a racemic mixture [1–4], as shown by studies of differential blood-m ...
CD4+ T-Cell-Independent Secondary Immune Responses to
... Pneumocystis infection upon adoptive transfer into Rag1(−/−) mice (3). As such, CD4+ T-cells have been a primary focus in the study of host defense against this pathogen. However, it has also been shown that adoptive transfer of hyperimmune serum mediates effective passive immunity to Pneumocystis i ...
... Pneumocystis infection upon adoptive transfer into Rag1(−/−) mice (3). As such, CD4+ T-cells have been a primary focus in the study of host defense against this pathogen. However, it has also been shown that adoptive transfer of hyperimmune serum mediates effective passive immunity to Pneumocystis i ...
The plant genome`s methylation status and response to stress
... isomerase genes (PAI1 and PAI4) form an inverted repeat. Transcription of this repeat generates a double-stranded RNA (dsRNA) signal for cytosine methylation and causes the methylation of the repeat itself and of two unlinked PAI genes (PAI2 and PAI3) [30,31]. Within a segregating population derived ...
... isomerase genes (PAI1 and PAI4) form an inverted repeat. Transcription of this repeat generates a double-stranded RNA (dsRNA) signal for cytosine methylation and causes the methylation of the repeat itself and of two unlinked PAI genes (PAI2 and PAI3) [30,31]. Within a segregating population derived ...
Epigenetic mechanisms regulate placental c-myc
... phenotypic behavior of placental trophoblasts is highly regulated, unlike most tumor tissues, involving a highly complex differential global gene expression profile varying with gestational age. The importance of this regulatory mechanism in placental development becomes clearer in the case of aberra ...
... phenotypic behavior of placental trophoblasts is highly regulated, unlike most tumor tissues, involving a highly complex differential global gene expression profile varying with gestational age. The importance of this regulatory mechanism in placental development becomes clearer in the case of aberra ...
Surveying Saccharomyces Genomes to Identify Functional Elements
... sequences within proteins, but has been less useful for identifying functional sequence elements in nonprotein-coding DNA because the relatively rapid rate of change of nonprotein-coding sequences and the relative simplicity of non-coding regulatory sequence elements necessitates the comparison of s ...
... sequences within proteins, but has been less useful for identifying functional sequence elements in nonprotein-coding DNA because the relatively rapid rate of change of nonprotein-coding sequences and the relative simplicity of non-coding regulatory sequence elements necessitates the comparison of s ...
A-level Biology Previous essay titles and mark schemes
... transformed organisms that benefit humans Other uses of DNA Cell cycle and treatment of cancer Gene therapy; Medical diagnosis and the treatment of human disease; The use of DNA probes to screen patients for clinically important genes. ...
... transformed organisms that benefit humans Other uses of DNA Cell cycle and treatment of cancer Gene therapy; Medical diagnosis and the treatment of human disease; The use of DNA probes to screen patients for clinically important genes. ...
DNA vaccination
DNA vaccination is a technique for protecting an animal against disease by injecting it with genetically engineered DNA so cells directly produce an antigen, resulting in a protective immunological response. Several DNA vaccines have been released for veterinary use, and there has been promising research using the vaccines for viral, bacterial and parasitic diseases, as well as to several tumour types. Although only one DNA vaccine has been approved for human use, DNA vaccines may have a number of potential advantages over conventional vaccines, including the ability to induce a wider range of immune response types.