Binding of the EcoRII methyltransferase to 5
... EcoRII-DNA adduct with staphylococcal protease V8 by high pressure liquid chromatography and polyacrylamide gel electrophoresis. Sequencing of the peptide indicated the DNA bound to a region of the protein that is conserved in all procaryotic DNA(cytosine-5)methyltransferases. We have previously sho ...
... EcoRII-DNA adduct with staphylococcal protease V8 by high pressure liquid chromatography and polyacrylamide gel electrophoresis. Sequencing of the peptide indicated the DNA bound to a region of the protein that is conserved in all procaryotic DNA(cytosine-5)methyltransferases. We have previously sho ...
Leukaemia Section t(11;11)(q13;q23) Atlas of Genetics and Cytogenetics in Oncology and Haematology
... control of her karyotype revealed a t(11;11)(q13;q23), in the absence of any sign of leukemia in the bone marrow, over a period of 30 months following the discover of the t(11;11). Other cases of t(11;11)(q13;q23) were: A 13-year-old girl, who have had a M4eo AML with inv(16)(p13q22). ...
... control of her karyotype revealed a t(11;11)(q13;q23), in the absence of any sign of leukemia in the bone marrow, over a period of 30 months following the discover of the t(11;11). Other cases of t(11;11)(q13;q23) were: A 13-year-old girl, who have had a M4eo AML with inv(16)(p13q22). ...
Roles of the Amino Group of Purine Bases in the Thermodynamic
... a conformation that did not alter the overall B-form of the DNA helix. These spectra were also insensitive to the salt concentration and solvent composition investigated in this study (Figure S1). The CD spectral data suggested that the differences in thermodynamic stabilities among the duplexes res ...
... a conformation that did not alter the overall B-form of the DNA helix. These spectra were also insensitive to the salt concentration and solvent composition investigated in this study (Figure S1). The CD spectral data suggested that the differences in thermodynamic stabilities among the duplexes res ...
How Does Replication-Associated Mutational Pressure Influence
... 1997; Kunst et al. 1997). Conversely, some experiments have proved that the frequency of mutations introduced into the nontranscribed DNA strand is higher than those in the transcribed strand (Francino et al. 1996). Replication is thought to be another cause of ...
... 1997; Kunst et al. 1997). Conversely, some experiments have proved that the frequency of mutations introduced into the nontranscribed DNA strand is higher than those in the transcribed strand (Francino et al. 1996). Replication is thought to be another cause of ...
Deep Insight Section Common fragile sites and genomic instability
... that take place within domains spatially and temporally separated (Wei et al., 1998). Usually transcription occurs in G1 phase and sometimes in S phase. When this happens, transcription is thought to be spatially separated from replication sites (Vieira et al., 2004). Gene expression induction in ma ...
... that take place within domains spatially and temporally separated (Wei et al., 1998). Usually transcription occurs in G1 phase and sometimes in S phase. When this happens, transcription is thought to be spatially separated from replication sites (Vieira et al., 2004). Gene expression induction in ma ...
Sample Chapter - McGraw Hill Higher Education
... has a molecular structure that resembles two linked rings. The other two bases— cytosine (C) and thymine (T)—are called pyrimidines. They each have a molecular structure consisting of a single ring. The nucleotides in a DNA molecule are bonded to each other in such a way that they form a chain that ...
... has a molecular structure that resembles two linked rings. The other two bases— cytosine (C) and thymine (T)—are called pyrimidines. They each have a molecular structure consisting of a single ring. The nucleotides in a DNA molecule are bonded to each other in such a way that they form a chain that ...
Leukaemia Section t(11;19)(q23;p13.1) Atlas of Genetics and Cytogenetics in Oncology and Haematology
... Note: Two different translocations (and two clinical entities), both involving 11q23 with a common breakpoint in MLL, and 19p13 with different breakpoints are now identified: the above mentioned, and the t(11;19)(q23;p13.3). ...
... Note: Two different translocations (and two clinical entities), both involving 11q23 with a common breakpoint in MLL, and 19p13 with different breakpoints are now identified: the above mentioned, and the t(11;19)(q23;p13.3). ...
Agrobacterium-mediated DNA transfer, and then some
... has raised the possibility that antibiotic resistance genes on the plasmid might be transferred to plants. T-DNA–encoded genes are transcribed from typical eukaryotic promoters. However, transcription in plants of bacterial chromosomal genes from prokaryotic promoters would be problematic. The likel ...
... has raised the possibility that antibiotic resistance genes on the plasmid might be transferred to plants. T-DNA–encoded genes are transcribed from typical eukaryotic promoters. However, transcription in plants of bacterial chromosomal genes from prokaryotic promoters would be problematic. The likel ...
Selick, H.E., Barry, J., Cha, T. - Bruce Alberts
... The 32 protein also stimuiates synthesis by the polym erase on a primed single-stranded temrlate, and it seems to exert part of its effect through a direct interaction with the T4 DNA polymerase (21,22). The addition of gene 41 protein to the above five-pro tein “core replication system” makes possi ...
... The 32 protein also stimuiates synthesis by the polym erase on a primed single-stranded temrlate, and it seems to exert part of its effect through a direct interaction with the T4 DNA polymerase (21,22). The addition of gene 41 protein to the above five-pro tein “core replication system” makes possi ...
Discussion S1.
... additional support, a number of interesting interactions were validated by coimmunoprecipitation (Figure 4B). Several known functional complexes can be identified including the Uvr-system (involved in base-excision repair), the Ruv-system (resolves recombination junctions), and the DNA replication c ...
... additional support, a number of interesting interactions were validated by coimmunoprecipitation (Figure 4B). Several known functional complexes can be identified including the Uvr-system (involved in base-excision repair), the Ruv-system (resolves recombination junctions), and the DNA replication c ...
MSLs Cumulative Review
... Cells that become the colored part of the eye are passed from parents to children. Molecules that contain the information that determines eye color are passed from parents to children. Nothing having anything to do with eye color is passed from parents to children. ...
... Cells that become the colored part of the eye are passed from parents to children. Molecules that contain the information that determines eye color are passed from parents to children. Nothing having anything to do with eye color is passed from parents to children. ...
A Dnmt2-like protein mediates DNA methylation in
... discussed controversially for a long period of time. There are several reports that demonstrate the absence of 5methylcytosine from pupal and adult stages of fly development (Patel and Gopinathan, 1987; Tweedie et al., 1999). In addition, it has also been shown that DNA from Drosophila embryos is la ...
... discussed controversially for a long period of time. There are several reports that demonstrate the absence of 5methylcytosine from pupal and adult stages of fly development (Patel and Gopinathan, 1987; Tweedie et al., 1999). In addition, it has also been shown that DNA from Drosophila embryos is la ...
DNA Technology: What is it? Technology is the practical use of
... Technology is the practical use of Scientific knowledge; so DNA Technology is using what we know about the structure and functioning of DNA to improve life through forensics(solving crimes/mysteries), bioinformatics, pharmacology/nanotechnology (creating solutions for health and nutrition) Start by ...
... Technology is the practical use of Scientific knowledge; so DNA Technology is using what we know about the structure and functioning of DNA to improve life through forensics(solving crimes/mysteries), bioinformatics, pharmacology/nanotechnology (creating solutions for health and nutrition) Start by ...
2 An Overview of Nucleic Acid Chemistry, Structure, and Function
... polynucleotide strands that are coiled about one another in a spiral (3,4). Each polynucleotide strand is held together by phosphodiester bonds linking adjacent deoxyribose moieties. The two polynucleotide strands are held together by a variety of noncovalent interactions, including lipophilic inter ...
... polynucleotide strands that are coiled about one another in a spiral (3,4). Each polynucleotide strand is held together by phosphodiester bonds linking adjacent deoxyribose moieties. The two polynucleotide strands are held together by a variety of noncovalent interactions, including lipophilic inter ...
A Flow Cytometry Approach to Detect In vivo Chromatin Compaction
... The chromatin condensation of soybean leaf samples from five different development stages, those are from shoot apical meristem (SAM), folded trifoliate, half-expanded trifoliate, fullyexpanded trifoliate to matured and old (3 months) leaves were tested by this method. Non staining samples were used ...
... The chromatin condensation of soybean leaf samples from five different development stages, those are from shoot apical meristem (SAM), folded trifoliate, half-expanded trifoliate, fullyexpanded trifoliate to matured and old (3 months) leaves were tested by this method. Non staining samples were used ...
(FA-SAT) in a Cat Fibrosarcoma Might Be Related to Chromosomal
... numerical chromosomal alterations. Further segmental chromosomal gains and losses come from structural chromosomal alterations, including reciprocal and nonreciprocal translocations, homogeneously staining regions, amplifications, insertions, and deletions. Structural alterations may result in a fur ...
... numerical chromosomal alterations. Further segmental chromosomal gains and losses come from structural chromosomal alterations, including reciprocal and nonreciprocal translocations, homogeneously staining regions, amplifications, insertions, and deletions. Structural alterations may result in a fur ...
The Mammalian Mismatch Repair Pathway Removes DNA 8
... MEF extracts (Figure 3B). There were no detectable differences among the Msh2-proficient and -defective variants of Colo26 in this regard. It is unclear why elevated levels of DNA 8-oxoG coexist with a high Ogg-1 activity in these mouse tumor cells. It may reflect unusual kinetics of the Ogg-1-media ...
... MEF extracts (Figure 3B). There were no detectable differences among the Msh2-proficient and -defective variants of Colo26 in this regard. It is unclear why elevated levels of DNA 8-oxoG coexist with a high Ogg-1 activity in these mouse tumor cells. It may reflect unusual kinetics of the Ogg-1-media ...
A common mechanism for ATP hydrolysis in ABC transporter and
... Both helicases and ABC proteins contain two wellcharacterized ATP-binding signatures in their primary structures, known as the Walker A- and B-motifs15. Initially, the A-motif, A/Gx4GKT/S, was found in some ATP-utilizing enzymes; for example, RecA, the α and β subunits of F1-ATPase, and myosin15. Th ...
... Both helicases and ABC proteins contain two wellcharacterized ATP-binding signatures in their primary structures, known as the Walker A- and B-motifs15. Initially, the A-motif, A/Gx4GKT/S, was found in some ATP-utilizing enzymes; for example, RecA, the α and β subunits of F1-ATPase, and myosin15. Th ...
From Genetics to DNA
... of guanosine, as well as double-strand breaks. It has been estimated that in each human cell, about 500 bases suffer oxidative damage per day. Of these oxidative lesions, the most dangerous are double-strand breaks, as these lesions are difficult to repair and can produce point mutations, insertions ...
... of guanosine, as well as double-strand breaks. It has been estimated that in each human cell, about 500 bases suffer oxidative damage per day. Of these oxidative lesions, the most dangerous are double-strand breaks, as these lesions are difficult to repair and can produce point mutations, insertions ...
The Effects of Plasmid on Genotype and Phenotype
... plasmid carrying this gene, then the bacteria can grow in the presence of ampicillin and are said to have the phenotype "ampicillin-resistant". The plasmid you will be using in your experiments is a plasmid constructed by scientists for use in recombinant DNA experiments. The beta-lactamase gene was ...
... plasmid carrying this gene, then the bacteria can grow in the presence of ampicillin and are said to have the phenotype "ampicillin-resistant". The plasmid you will be using in your experiments is a plasmid constructed by scientists for use in recombinant DNA experiments. The beta-lactamase gene was ...
Differential chromatin packaging of genomic
... and one is the CpG methylation. Almost all imprinted genes have sequence elements that are methylated on only one of the two parental alleles. The differential methylation is a signal that leads to an inactive state of chromatin probably through binding to methyl-CpG-binding proteins, such as MeCP2, ...
... and one is the CpG methylation. Almost all imprinted genes have sequence elements that are methylated on only one of the two parental alleles. The differential methylation is a signal that leads to an inactive state of chromatin probably through binding to methyl-CpG-binding proteins, such as MeCP2, ...
iGenetics: A Molecular Approach, 3e (Russell/Bose)
... Comparison of the genomes of different dog breeds may therefore shed light on which genetic elements are responsible for the radically different developmental pathways of different dog breeds–a process that may represent a microcosm of species-level differentiation over evolutionary time. Skill: Con ...
... Comparison of the genomes of different dog breeds may therefore shed light on which genetic elements are responsible for the radically different developmental pathways of different dog breeds–a process that may represent a microcosm of species-level differentiation over evolutionary time. Skill: Con ...
ods of time, until activated b), a activated, the I`irtrl DNA hiiacks the
... co.mpenents: a nitrogenous base (in DNA, A, C, T, or G), a sugar (blue), and a phosphate group (yellow). The nucleotides are joined to one another by covalent'bonds between the sggqq of _on9 nuClgotide and the phosphate of the next. This results in a sugar-phosphate backbone, a repeating pattern of ...
... co.mpenents: a nitrogenous base (in DNA, A, C, T, or G), a sugar (blue), and a phosphate group (yellow). The nucleotides are joined to one another by covalent'bonds between the sggqq of _on9 nuClgotide and the phosphate of the next. This results in a sugar-phosphate backbone, a repeating pattern of ...
Nucleosome
A nucleosome is a basic unit of DNA packaging in eukaryotes, consisting of a segment of DNA wound in sequence around eight histone protein cores. This structure is often compared to thread wrapped around a spool.Nucleosomes form the fundamental repeating units of eukaryotic chromatin, which is used to pack the large eukaryotic genomes into the nucleus while still ensuring appropriate access to it (in mammalian cells approximately 2 m of linear DNA have to be packed into a nucleus of roughly 10 µm diameter). Nucleosomes are folded through a series of successively higher order structures to eventually form a chromosome; this both compacts DNA and creates an added layer of regulatory control, which ensures correct gene expression. Nucleosomes are thought to carry epigenetically inherited information in the form of covalent modifications of their core histones.Nucleosomes were observed as particles in the electron microscope by Don and Ada Olins and their existence and structure (as histone octamers surrounded by approximately 200 base pairs of DNA) were proposed by Roger Kornberg. The role of the nucleosome as a general gene repressor was demonstrated by Lorch et al. in vitro and by Han and Grunstein in vivo.The nucleosome core particle consists of approximately 147 base pairs of DNA wrapped in 1.67 left-handed superhelical turns around a histone octamer consisting of 2 copies each of the core histones H2A, H2B, H3, and H4. Core particles are connected by stretches of ""linker DNA"", which can be up to about 80 bp long. Technically, a nucleosome is defined as the core particle plus one of these linker regions; however the word is often synonymous with the core particle. Genome-wide nucleosome positioning maps are now available for many model organisms including mouse liver and brain.Linker histones such as H1 and its isoforms are involved in chromatin compaction and sit at the base of the nucleosome near the DNA entry and exit binding to the linker region of the DNA. Non-condensed nucleosomes without the linker histone resemble ""beads on a string of DNA"" under an electron microscope.In contrast to most eukaryotic cells, mature sperm cells largely use protamines to package their genomic DNA, most likely to achieve an even higher packaging ratio. Histone equivalents and a simplified chromatin structure have also been found in Archea, suggesting that eukaryotes are not the only organisms that use nucleosomes.