2009 - Barley World
... progeny of the cross between two completely inbred lines will be a. 1:1 b. 1:2:1 c. 3:1 d. 9:3:3:1 33. The highest temperature steps in a PCR reaction are necessary for a. denaturation of the DNA. b. primer annealing. c. primer extension. d. cutting at palindromic sequences. 34. RFLPs are a. a type ...
... progeny of the cross between two completely inbred lines will be a. 1:1 b. 1:2:1 c. 3:1 d. 9:3:3:1 33. The highest temperature steps in a PCR reaction are necessary for a. denaturation of the DNA. b. primer annealing. c. primer extension. d. cutting at palindromic sequences. 34. RFLPs are a. a type ...
Biotechnology - The Bio Edge
... 28. When electrical current is applied during a gel electrophoresis procedure, the DNA fragments are separated by A. B. C. D. E. ...
... 28. When electrical current is applied during a gel electrophoresis procedure, the DNA fragments are separated by A. B. C. D. E. ...
BioSc 231 2001 Exam4
... _____ Clusters of highly repetitive DNA located near the centromeres and telomeres are called A. Nucleosomes B. Euchromatin C. Chromatids D. Heterochromatin E. 30 nm chromatin _____ Which histone protein is present as a monomer within the nucleosome and is not a part of the core particle? A. H1 B. H ...
... _____ Clusters of highly repetitive DNA located near the centromeres and telomeres are called A. Nucleosomes B. Euchromatin C. Chromatids D. Heterochromatin E. 30 nm chromatin _____ Which histone protein is present as a monomer within the nucleosome and is not a part of the core particle? A. H1 B. H ...
AP Biology Review Chapters 11-12 Review Questions Chapter 11
... f) Punnett squares give POSSIBLE outcomes in the offspring. Are the resultant offspring always in the numbers predicted? If a 3:1 ratio was predicted for a trait and a child is born with the recessive trait, what is the probability that the next child will be born with the recessive trait? g) Be abl ...
... f) Punnett squares give POSSIBLE outcomes in the offspring. Are the resultant offspring always in the numbers predicted? If a 3:1 ratio was predicted for a trait and a child is born with the recessive trait, what is the probability that the next child will be born with the recessive trait? g) Be abl ...
BIOTECHNOLOGY
... occurring enzymes that act like a pair of molecular scissors to cut DNA in a predictable and precise manner, at a specific nucleotide sequence called a recognition site . Hamilton Smith, John Hopkins University, won the Nobel Prize in 1978 for discovering restriction enzymes in bacteria (Hind III). ...
... occurring enzymes that act like a pair of molecular scissors to cut DNA in a predictable and precise manner, at a specific nucleotide sequence called a recognition site . Hamilton Smith, John Hopkins University, won the Nobel Prize in 1978 for discovering restriction enzymes in bacteria (Hind III). ...
Biology Honors Final Review
... 2. If an animal cell is placed in a hypertonic solution, which way does the water move? In a hypotonic solution? In an isotonic solution? 3. What organelle regulates what gets into the cell? 4. Describe exocytosis and endocytosis. Why are these processes important to a cell? Unit: 5 1. What types o ...
... 2. If an animal cell is placed in a hypertonic solution, which way does the water move? In a hypotonic solution? In an isotonic solution? 3. What organelle regulates what gets into the cell? 4. Describe exocytosis and endocytosis. Why are these processes important to a cell? Unit: 5 1. What types o ...
GENETICS EXAM 3 FALL 2004 Student Name
... been cut with the restriction enzyme SfoI? Arrows indicate where the enzymes cut DNA. Circle each correct answer. SfoI cuts the following sequence: GGCGCC CCGCGG #1 GGCGCC CCGCGG ...
... been cut with the restriction enzyme SfoI? Arrows indicate where the enzymes cut DNA. Circle each correct answer. SfoI cuts the following sequence: GGCGCC CCGCGG #1 GGCGCC CCGCGG ...
Document
... Automated sequencing machines, particularly those made by PE Applied Biosystems, use 4 colors, so they can read all 4 bases at once. ...
... Automated sequencing machines, particularly those made by PE Applied Biosystems, use 4 colors, so they can read all 4 bases at once. ...
BLAST - Georgia State University
... – The goal is to maximize Score(s,DNA) by varying the starting positions si, where: ...
... – The goal is to maximize Score(s,DNA) by varying the starting positions si, where: ...
Prokaryotes, Viruses, and Protistans
... • Bacteria are the smallest living organisms • Viruses are smaller but are not alive ...
... • Bacteria are the smallest living organisms • Viruses are smaller but are not alive ...
DNA - Biology
... lengths of the VNTR regions can then be determined. The apparatus for a gel electrophoresis consists of a container filled with a porous agarose gel. An electrical current is run through the gel and, since DNA is slightly negatively charged, the fragments will move through the pores in the gel at di ...
... lengths of the VNTR regions can then be determined. The apparatus for a gel electrophoresis consists of a container filled with a porous agarose gel. An electrical current is run through the gel and, since DNA is slightly negatively charged, the fragments will move through the pores in the gel at di ...
Cellular Control
... this prevents RNA polymerase binding whilst the other site binds with lactose, when bound it changes the shape of the repressor protein so that it no longer fits onto the Operator DNA so it would free RNA polymerase to transcribe ...
... this prevents RNA polymerase binding whilst the other site binds with lactose, when bound it changes the shape of the repressor protein so that it no longer fits onto the Operator DNA so it would free RNA polymerase to transcribe ...
X-Sheet 2 Protein Synthesis and DNA Fingerprinting
... being the order of the nitrogenous base sequences. A DNA profile is designed by using DNA probes. A selection of DNA sequences within the DNA profile forms what is termed the VNTR pattern for that individual. Forensic scientists are able to compare the DNA profiles to a sample that is provided from ...
... being the order of the nitrogenous base sequences. A DNA profile is designed by using DNA probes. A selection of DNA sequences within the DNA profile forms what is termed the VNTR pattern for that individual. Forensic scientists are able to compare the DNA profiles to a sample that is provided from ...
BACTERIA TRANSFORMATION LAB (ACTIVITY)
... a bacterial plasmid, and then cut these two DNA molecules into fragments using special enzymes called restriction enzymes. The DNA fragments are spliced together with an enzyme called ligase. Finally the engineered plasmid is taken up by a bacterial cell for replication and expression of the inserte ...
... a bacterial plasmid, and then cut these two DNA molecules into fragments using special enzymes called restriction enzymes. The DNA fragments are spliced together with an enzyme called ligase. Finally the engineered plasmid is taken up by a bacterial cell for replication and expression of the inserte ...
Chapter 8: Microbial Genetics
... – Cause electrons to pop out of their usual shells – Ions can combine with bases in DNA, resulting in errors in DNA – Breakage of covalent bonds in sugar-phosphate backbonebreaks in chromosomes ...
... – Cause electrons to pop out of their usual shells – Ions can combine with bases in DNA, resulting in errors in DNA – Breakage of covalent bonds in sugar-phosphate backbonebreaks in chromosomes ...
Outlines_Ch16
... autophosphorylated on histidine when it binds an inducer. • VirA activates VirG by transferring the phosphate group to it. • The VirA-VirG is one of several bacterial two component systems that use a phosphohistidine relay. ...
... autophosphorylated on histidine when it binds an inducer. • VirA activates VirG by transferring the phosphate group to it. • The VirA-VirG is one of several bacterial two component systems that use a phosphohistidine relay. ...
Nucleosome
A nucleosome is a basic unit of DNA packaging in eukaryotes, consisting of a segment of DNA wound in sequence around eight histone protein cores. This structure is often compared to thread wrapped around a spool.Nucleosomes form the fundamental repeating units of eukaryotic chromatin, which is used to pack the large eukaryotic genomes into the nucleus while still ensuring appropriate access to it (in mammalian cells approximately 2 m of linear DNA have to be packed into a nucleus of roughly 10 µm diameter). Nucleosomes are folded through a series of successively higher order structures to eventually form a chromosome; this both compacts DNA and creates an added layer of regulatory control, which ensures correct gene expression. Nucleosomes are thought to carry epigenetically inherited information in the form of covalent modifications of their core histones.Nucleosomes were observed as particles in the electron microscope by Don and Ada Olins and their existence and structure (as histone octamers surrounded by approximately 200 base pairs of DNA) were proposed by Roger Kornberg. The role of the nucleosome as a general gene repressor was demonstrated by Lorch et al. in vitro and by Han and Grunstein in vivo.The nucleosome core particle consists of approximately 147 base pairs of DNA wrapped in 1.67 left-handed superhelical turns around a histone octamer consisting of 2 copies each of the core histones H2A, H2B, H3, and H4. Core particles are connected by stretches of ""linker DNA"", which can be up to about 80 bp long. Technically, a nucleosome is defined as the core particle plus one of these linker regions; however the word is often synonymous with the core particle. Genome-wide nucleosome positioning maps are now available for many model organisms including mouse liver and brain.Linker histones such as H1 and its isoforms are involved in chromatin compaction and sit at the base of the nucleosome near the DNA entry and exit binding to the linker region of the DNA. Non-condensed nucleosomes without the linker histone resemble ""beads on a string of DNA"" under an electron microscope.In contrast to most eukaryotic cells, mature sperm cells largely use protamines to package their genomic DNA, most likely to achieve an even higher packaging ratio. Histone equivalents and a simplified chromatin structure have also been found in Archea, suggesting that eukaryotes are not the only organisms that use nucleosomes.