7.2mb ppt - UCLA.edu
... deaminase (AID), which is expressed specifically in germinal-centre centroblasts1, is required for this process, but its exact role is unknown2. Here we show that AID is required for SHM in the centroblast-like Ramos cells, and that expression of AID is sufficient to induce SHM in hybridoma cells, w ...
... deaminase (AID), which is expressed specifically in germinal-centre centroblasts1, is required for this process, but its exact role is unknown2. Here we show that AID is required for SHM in the centroblast-like Ramos cells, and that expression of AID is sufficient to induce SHM in hybridoma cells, w ...
GENETICS PROBLEMS: Include the appropriate Punnett Squares to
... 9. Muscular dystrophy is a sex-linked recessive disorder. A man with muscular dystrophy marries a woman who does not have this disease. Half of their daughters and half of their sons develop this disease. What is the genotype of the father? The mother? Document how you arrived at your answer or rece ...
... 9. Muscular dystrophy is a sex-linked recessive disorder. A man with muscular dystrophy marries a woman who does not have this disease. Half of their daughters and half of their sons develop this disease. What is the genotype of the father? The mother? Document how you arrived at your answer or rece ...
40. Bacterial Transformation Lab Notebook TEACHER
... the scissors and tape with petri dishes, chemicals, thermal processes, and scientific equipment. As you complete the problem, record your protocol and results. Problem: Because previous types of insulin production caused adverse reactions in patients, alternate production methods were necessary. Thr ...
... the scissors and tape with petri dishes, chemicals, thermal processes, and scientific equipment. As you complete the problem, record your protocol and results. Problem: Because previous types of insulin production caused adverse reactions in patients, alternate production methods were necessary. Thr ...
Nucleolar Dominance - Indiana University Bloomington
... sequencing ladders used as size markers. Note that 5-aza-20 deoxycytosine and trichostatin A together are not signi®cantly more effective at derepressing the B. oleracea genes than is either chemical alone, suggesting that DNA methylation and histone deacetylation are partners in the same repression ...
... sequencing ladders used as size markers. Note that 5-aza-20 deoxycytosine and trichostatin A together are not signi®cantly more effective at derepressing the B. oleracea genes than is either chemical alone, suggesting that DNA methylation and histone deacetylation are partners in the same repression ...
et al. MATERIALS AND METHODS Construction of the synthetic network
... protein life-time. It is assumed that the effective protein and plasmid life-times are dominated by dilution due to cell growth and therefore are determined by the cell doubling time. The coefficients presented here correspond to static global noise. We presented our results using this simple assump ...
... protein life-time. It is assumed that the effective protein and plasmid life-times are dominated by dilution due to cell growth and therefore are determined by the cell doubling time. The coefficients presented here correspond to static global noise. We presented our results using this simple assump ...
Mary Ann Osley*, Alastair Fleming, and Cheng
... yeast H2B. This duplication of effort appears to target ub-H2B in different regions of chromatin for deubiquitylation, and may constitute a general mechanism for deubiquitylation of the same protein when it is present in different cellular compartments. II.2 Factors regulating histone ubquitylation ...
... yeast H2B. This duplication of effort appears to target ub-H2B in different regions of chromatin for deubiquitylation, and may constitute a general mechanism for deubiquitylation of the same protein when it is present in different cellular compartments. II.2 Factors regulating histone ubquitylation ...
P D G E
... quantity of specific mRNA of interest that is present in a sample (the target). The molecules in the target are labelled, and a specialised scanner is used to measure the amount of hybridisation (intensity) of the target at each probe. Gene intensity values are recorded for a number of microarray ex ...
... quantity of specific mRNA of interest that is present in a sample (the target). The molecules in the target are labelled, and a specialised scanner is used to measure the amount of hybridisation (intensity) of the target at each probe. Gene intensity values are recorded for a number of microarray ex ...
Recent Statistical Approaches
... type I error (false positive) increases sharply with the number of hypotheses. ...
... type I error (false positive) increases sharply with the number of hypotheses. ...
Recurrent Tandem Gene Duplication Gave Rise
... process was simulated using 2,000 replicates with the number of segregating sites set to that observed in the data. However, these approaches, based on the polymorphic spectrum, are of limited utility in testing for neutrality in young genes because a reduced level of diversity and skew toward rare ...
... process was simulated using 2,000 replicates with the number of segregating sites set to that observed in the data. However, these approaches, based on the polymorphic spectrum, are of limited utility in testing for neutrality in young genes because a reduced level of diversity and skew toward rare ...
Caspary T, Cleary MA, Baker CC, Guan XJ, Tilghman SM. Mol Cell Biol. 1998 Jun;18(6):3466-74. Multiple mechanisms of imprinting on distal mouse chromosome 7.
... contains a cluster of four maternally expressed genes, H19, Mash2, Kvlqt1, and p57Kip2, as well as two paternally expressed genes, Igf2 and Ins2, and assess the expression and imprinting of Mash2, Kvlqt1, and p57Kip2 during development in embryonic and extraembryonic tissues. Unlike Igf2 and Ins2, w ...
... contains a cluster of four maternally expressed genes, H19, Mash2, Kvlqt1, and p57Kip2, as well as two paternally expressed genes, Igf2 and Ins2, and assess the expression and imprinting of Mash2, Kvlqt1, and p57Kip2 during development in embryonic and extraembryonic tissues. Unlike Igf2 and Ins2, w ...
Respiratory Epithelial Gene Expression in Patients with Mild and
... RNA of sufficient quantity or quality after purification to permit microarray analysis. The remaining 37 samples were hybridized to Affymetrix Human Genome HG-U133 A and B microarrays. Twenty-three of the U133A arrays and 21 of the matching U133B arrays passed the stringent quality control guidelines ...
... RNA of sufficient quantity or quality after purification to permit microarray analysis. The remaining 37 samples were hybridized to Affymetrix Human Genome HG-U133 A and B microarrays. Twenty-three of the U133A arrays and 21 of the matching U133B arrays passed the stringent quality control guidelines ...
Gene Section ANXA1 (annexin A1) Atlas of Genetics and Cytogenetics
... Laryngeal squamous cell carcinoma Disease In surgical tissue specimens from 20 patients with laryngeal squamous cell carcinoma, ultrastructural immunocytochemistry analysis showed in vivo downregulation of ANXA1 expression in the tumor and increased in mast cells and laryngeal squamous carcinoma cel ...
... Laryngeal squamous cell carcinoma Disease In surgical tissue specimens from 20 patients with laryngeal squamous cell carcinoma, ultrastructural immunocytochemistry analysis showed in vivo downregulation of ANXA1 expression in the tumor and increased in mast cells and laryngeal squamous carcinoma cel ...
The possibilities of practical application of transgenic mammalian
... also detected in the brain, kidneys and testis of some transgenic mouse lines and murine cell lines (Lin et al. 1995, Kerr et al. 1998, Zhang et al. 2002, Zhu et al. 2004, Kwon et al. 2006). Kwon et al. (2006) cloned an 8.8 kb pig UPII (pUPII) promoter region and investigated which cells within the ...
... also detected in the brain, kidneys and testis of some transgenic mouse lines and murine cell lines (Lin et al. 1995, Kerr et al. 1998, Zhang et al. 2002, Zhu et al. 2004, Kwon et al. 2006). Kwon et al. (2006) cloned an 8.8 kb pig UPII (pUPII) promoter region and investigated which cells within the ...
MtHap_GWA_README
... The association analysis is divided into two steps that can be run through a single shell script (tassel_shell.pbs, this example is run as a batch job at the Minnesota Supercomputing Institute. Exact details are likely to differ for other servers). First, TASSEL is called by command line to perform ...
... The association analysis is divided into two steps that can be run through a single shell script (tassel_shell.pbs, this example is run as a batch job at the Minnesota Supercomputing Institute. Exact details are likely to differ for other servers). First, TASSEL is called by command line to perform ...
Microarray statistical validation and functional annotation
... If the stringency of the statistical validation is too high biologically meaningful genes can be lost making more difficult to role out functional correlations between the differentially expressed genes. If the stringency of the statistical validation is too loose the increase of false positives cre ...
... If the stringency of the statistical validation is too high biologically meaningful genes can be lost making more difficult to role out functional correlations between the differentially expressed genes. If the stringency of the statistical validation is too loose the increase of false positives cre ...
Overexpression of DNA repair genes is associated with metastasis
... gene expression signatures have been reported for primary tumours with metastatic capacity suggesting that most, if not all, of these primary tumour cells exhibit metastatic potency [1,3,4]. In several cases where this has been searched, the metastasis and primary tumour in the same patient show sim ...
... gene expression signatures have been reported for primary tumours with metastatic capacity suggesting that most, if not all, of these primary tumour cells exhibit metastatic potency [1,3,4]. In several cases where this has been searched, the metastasis and primary tumour in the same patient show sim ...
Gene Section NF2 (neurofibromatosis type 2) Atlas of Genetics and Cytogenetics
... This article is an update of: Huret JL. NF2 (neurofibromatosis type 2). Atlas Genet Cytogenet Oncol Haematol.1998;2(2):39-40. ...
... This article is an update of: Huret JL. NF2 (neurofibromatosis type 2). Atlas Genet Cytogenet Oncol Haematol.1998;2(2):39-40. ...
Differential Gene Expression Differentially Expressed Genes
... The BH procedure is a step-up procedure that provides strong control of the FDR. The key to understanding/interpretation is to understand the meaning of the FDR. The FDR indicates the expected (average) proportion of ’discoveries’ (ie, rejected null hypotheses) that are ’false discoveries’ (ie, the ...
... The BH procedure is a step-up procedure that provides strong control of the FDR. The key to understanding/interpretation is to understand the meaning of the FDR. The FDR indicates the expected (average) proportion of ’discoveries’ (ie, rejected null hypotheses) that are ’false discoveries’ (ie, the ...
Hox gene regulation by C. elegans sop-3
... in developmental biology. Hox transcription factors constitute one class of transcriptional inputs whose role appears to be to convey positional cell identity (Gellon and McGinnis, 1998). Hox proteins interact with target promoters in combination with the co-factors extradenticle and homothorax, whi ...
... in developmental biology. Hox transcription factors constitute one class of transcriptional inputs whose role appears to be to convey positional cell identity (Gellon and McGinnis, 1998). Hox proteins interact with target promoters in combination with the co-factors extradenticle and homothorax, whi ...
New Certation Gene on the First Linkage Group Found
... of the cross of HO 639 with a Japanese marker line, and after F, of the cross between a Japanese tester and each of HO 1392 and HO 1405. Assuming that there are some correlation between the semi-sterility and the segregationdistortion in the above cases, it was examined whether or not the segregatio ...
... of the cross of HO 639 with a Japanese marker line, and after F, of the cross between a Japanese tester and each of HO 1392 and HO 1405. Assuming that there are some correlation between the semi-sterility and the segregationdistortion in the above cases, it was examined whether or not the segregatio ...
Leukaemia Section t(5;12)(p13;p1 3) NIPBL/ETV6 Atlas of Genetics and Cytogenetics
... Note The t(5;12)(p13;p13) involves the ETV6 gene (12p13), a transcription factor frequently rearranged in myeloid and lymphoid leukemias. More than 30 ETV6 fusion gene partners have been described. Most translocations involving ETV6 generate fusion genes that lead to the activation of transcription ...
... Note The t(5;12)(p13;p13) involves the ETV6 gene (12p13), a transcription factor frequently rearranged in myeloid and lymphoid leukemias. More than 30 ETV6 fusion gene partners have been described. Most translocations involving ETV6 generate fusion genes that lead to the activation of transcription ...
Chpt20_TxnlRegChromatin.doc
... nuclei. This shows that the amount of globin gene DNA in erythroid nuclei is substantially reduced by the DNase I treatment, i.e. the globin gene is sensitive to DNase I in a cell that is expressing it. {To put a finer touch on it, the erythrocytes are descended from cells that were actively express ...
... nuclei. This shows that the amount of globin gene DNA in erythroid nuclei is substantially reduced by the DNase I treatment, i.e. the globin gene is sensitive to DNase I in a cell that is expressing it. {To put a finer touch on it, the erythrocytes are descended from cells that were actively express ...
View/print full test page
... o Deletion/duplication analysis is performed using a high resolution, custom microarray platform designed to target the genes of interest at the exon level. Detection rates are limited to the genes specified; this test does not provide whole genome analysis. Gene panels are a more cost-effective app ...
... o Deletion/duplication analysis is performed using a high resolution, custom microarray platform designed to target the genes of interest at the exon level. Detection rates are limited to the genes specified; this test does not provide whole genome analysis. Gene panels are a more cost-effective app ...