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Genes and How they work!
Genes and How they work!

... about 250 A’s to 3’ end  long A tail. Needed to prevent degradation. ...
Comparative Genomics
Comparative Genomics

... are transcribed simultaneously. These genes were shown to share a promoter, much like prokaryotes control gene expression. ...
BioSc 231 2001 Exam5
BioSc 231 2001 Exam5

... B. is imposed by the position of cells in the embryo C. is mediated by physical interaction between cells D. is mediated by morphogens _____ Mutations that cause cells to undergo developmental fates characteristic of other types of cells are called A. execution mutations B. transformation mutations ...
Transcription Biology Review
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... • Proteins that line up several amino acid contacts bind strongly to specific DNA sequences ...
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... formation of new proteins RNA uses the DNA as a template to read the code in order to produce the right protein with the correct order and number of amino acids. ...
Chapter 17 Powerpoint
Chapter 17 Powerpoint

... The purpose of tRNA. • Interpreter of the mRNA codons is tRNA (transfer RNA) • Each tRNA molecule has a section called the anticodon. • tRNA grabs a specific amino acid & brings it to the ribosome ...
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... 9) Why is 15 bases the minimum for a oligo nucelotide design during a PCR experiment?________________________ 10) SNP is an abbreviation for______________________________? 11) Given random DNA , how frequent will the sequence TATA be found in the genome?________ 12) What three regions (not counting ...
RNA and Protein Synthesis
RNA and Protein Synthesis

... The amino acids form a peptide bond to hold them together. The next amino acid is brought in and is attached This continues until the ribosome reaches a stop codon The completed protein (polypeptide chain) is then released ...
Regulation of Gene Expression
Regulation of Gene Expression

... CAP mRNA synthesis Operon ? because CAP is bound and lac repressor is not ...
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Role for a bidentate ribonuclease in the initiation step of RNA
Role for a bidentate ribonuclease in the initiation step of RNA

... plants, fungi and mammals. The enzyme has a distinctive structure, which includes a helicase domain and dual RNase III motifs. Dicer also contains a region of homology to the RDE1/QDE2/ ARGONAUTE family that has been genetically linked to RNAi9,10. Biochemical studies have suggested that post-transc ...
Replication, Transcription, Translation
Replication, Transcription, Translation

... 1. Know the parts of a DNA nucleotide 2. Know the meaning o, and understand the process for the following words: replication, transcription, translation. 3. Know the respective sugars and nitrogenous bases that DNA and RNA contain. 4. Be able to name each of the 3 types of RNA and be able to explain ...
Chapter 12.5
Chapter 12.5

... GENE REGULATION: AN EXAMPLE • WE WILL USE E. COLI TO GIVE AN EXAMPLE OF TURNING A GENE ON AND OFF. • THE 4288 PROTEIN REGION IS MADE OF 3 GENES. THESE 3 GENES GET TURNED OFF AND ON TOGETHER. THESE GENES ARE RESPONSIBLE FOR BREAKING DOWN LACTOSE FOR THE BACTERIA. • A GROUP OF GENES THAT THAT TURN OF ...
New Microsoft Office PowerPoint Presentation
New Microsoft Office PowerPoint Presentation

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Gene Regulation - Marblehead High School
Gene Regulation - Marblehead High School

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Molecular Genetics - Lake Travis Independent School District
Molecular Genetics - Lake Travis Independent School District

... Nucleotides are made of ribose (instead of deoxyribose) RNA is single-stranded (DNA is double) The base Thymine is replaced with Uracil in RNA ...
AP Biology - Naber Biology
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... 4. Enzymatic pathways involve a series of different enzymes that catalyze reactions in sequence. In order for this to occur, the genes that code for these enzymes are coordinately controlled by being clustered in units known as operons. To better understand how an operon functions, begin by explaini ...
The Central Dogma of Molecular Biology
The Central Dogma of Molecular Biology

... The process of removing the intron is called splicing The intron is looped out and cut away from the exons by snRNPs (small nuclear ribonucleoprotein) (snurps) The exons are spliced together to produce the translatable mRNA The mRNA is now ready to leave the nucleus and be translated into protein ...
Transcription lesson
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... Initiation: Each gene of double stranded DNA has a sense strand and an antisense strand. The sense strand contains the “instructions” for protein synthesis. On the sense strand is a nucleotide sequence (called a promoter sequence) where RNA polymerase can bind. (RNA polymerase makes RNA) ...
Regulation of gene expression: Prokaryotic
Regulation of gene expression: Prokaryotic

... • Occurs when hairpin loops form from intramolecular GC base pairing in mRNA. • Sometimes a special protein called termination factor, rho is required for termination. ...
Nucleotide sequence of the gene encoding the
Nucleotide sequence of the gene encoding the

... complementary to the 3'-end of the 7.6 kbp PstI fragment. The product, 800 bp in length, contained the sequence encoding the missing C-terminus and the stop codon. The pol El A gene is 5205 bp long. Compared to other known pol III A genes it contains a 450 bp insert (from about position 1540 to posi ...
Biology 303 EXAM III
Biology 303 EXAM III

... Two types of post-transcriptional modifications that take place in the mRNA of eukaryotes are 1. the addition of a poly T sequence at the 5' end of the gene and the addition of a poly U tail at the 3' end. 2. addition of a poly A sequence at the 5' end and the addition of a “cap” at the 3' end of th ...
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... have DNA within a membrane bound compartment and prokaryotes do not? Could eukaryotes function without it? ...
Chapter 17 - HCC Learning Web
Chapter 17 - HCC Learning Web

... C) an enzyme that catalyzes the association between the large and small ribosomal subunits D) an enzyme that synthesizes RNA as part of the transcription process E) an enzyme that uses RNA as a substrate 5) During splicing, which molecular component of the spliceosome catalyzes the excision reaction ...
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RNA interference



RNA interference (RNAi) is a biological process in which RNA molecules inhibit gene expression, typically by causing the destruction of specific mRNA molecules. Historically, it was known by other names, including co-suppression, post-transcriptional gene silencing (PTGS), and quelling. Only after these apparently unrelated processes were fully understood did it become clear that they all described the RNAi phenomenon. Andrew Fire and Craig C. Mello shared the 2006 Nobel Prize in Physiology or Medicine for their work on RNA interference in the nematode worm Caenorhabditis elegans, which they published in 1998.Two types of small ribonucleic acid (RNA) molecules – microRNA (miRNA) and small interfering RNA (siRNA) – are central to RNA interference. RNAs are the direct products of genes, and these small RNAs can bind to other specific messenger RNA (mRNA) molecules and either increase or decrease their activity, for example by preventing an mRNA from producing a protein. RNA interference has an important role in defending cells against parasitic nucleotide sequences – viruses and transposons. It also influences development.The RNAi pathway is found in many eukaryotes, including animals, and is initiated by the enzyme Dicer, which cleaves long double-stranded RNA (dsRNA) molecules into short double-stranded fragments of ~20 nucleotide siRNAs. Each siRNA is unwound into two single-stranded RNAs (ssRNAs), the passenger strand and the guide strand. The passenger strand is degraded and the guide strand is incorporated into the RNA-induced silencing complex (RISC). The most well-studied outcome is post-transcriptional gene silencing, which occurs when the guide strand pairs with a complementary sequence in a messenger RNA molecule and induces cleavage by Argonaute, the catalytic component of the RISC complex. In some organisms, this process spreads systemically, despite the initially limited molar concentrations of siRNA.RNAi is a valuable research tool, both in cell culture and in living organisms, because synthetic dsRNA introduced into cells can selectively and robustly induce suppression of specific genes of interest. RNAi may be used for large-scale screens that systematically shut down each gene in the cell, which can help to identify the components necessary for a particular cellular process or an event such as cell division. The pathway is also used as a practical tool in biotechnology, medicine and insecticides.
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