Homologous Recombination (Introductory Concepts
... chromosome, but exchange between them cannot generate new gene combinations as the two chromosome copies are identical (unless mistakes in replication has generated mutations). However, the presence of an intact template copy of the genome is highly useful in salvaging by re ...
... chromosome, but exchange between them cannot generate new gene combinations as the two chromosome copies are identical (unless mistakes in replication has generated mutations). However, the presence of an intact template copy of the genome is highly useful in salvaging by re ...
The nucleotides
... the two strands wind around each other, forming a double helix. • eukaryotic cells, DNA is found associated with various types of proteins (known collectively as nucleoprotein) present in the nucleus, whereas in prokaryotes, the protein-DNA complex is present in the nucleoid. • Phosphodiester bonds ...
... the two strands wind around each other, forming a double helix. • eukaryotic cells, DNA is found associated with various types of proteins (known collectively as nucleoprotein) present in the nucleus, whereas in prokaryotes, the protein-DNA complex is present in the nucleoid. • Phosphodiester bonds ...
When replication travels on damaged templates: bumps and blocks
... replicative polymerase. In vitro, the translesion polymerase reaction also requires the activated form of RecA bound to the single-strand region, consistent with the structures and proteins that are present at replicated lesion-containing sites. In vivo, the absence of Pol V also modestly reduces th ...
... replicative polymerase. In vitro, the translesion polymerase reaction also requires the activated form of RecA bound to the single-strand region, consistent with the structures and proteins that are present at replicated lesion-containing sites. In vivo, the absence of Pol V also modestly reduces th ...
Identifying Stage-Specific Genes by Combining
... The identification of stage-specific genes provides a starting point to identify key regulatory elements and transcriptional regulators essential for the malaria parasite to complete its life cycle. It can provide for a better understanding of mechanisms responsible for the pathology or transmission ...
... The identification of stage-specific genes provides a starting point to identify key regulatory elements and transcriptional regulators essential for the malaria parasite to complete its life cycle. It can provide for a better understanding of mechanisms responsible for the pathology or transmission ...
Mutations in human pathology - diss.fu
... exon1390. When a mutation affects the splice donor site, this results in skipping of the upstream exon1393. Some nonsense mutations have also been reported to induce exon skipping1388. Sometimes, mutations can cause abnormal RNA splicing by activation of cryptic splice sites: a sequence which normal ...
... exon1390. When a mutation affects the splice donor site, this results in skipping of the upstream exon1393. Some nonsense mutations have also been reported to induce exon skipping1388. Sometimes, mutations can cause abnormal RNA splicing by activation of cryptic splice sites: a sequence which normal ...
Relative Expression of a Dominant Mutated ABCC8
... members. The methylation patterns of the ABCC8 promoter region and the KCNQ1 ICR (50%) were normal, regardless of the patient’s mutation or disease status (Fig. 3D and E) (some data not shown). DISCUSSION ...
... members. The methylation patterns of the ABCC8 promoter region and the KCNQ1 ICR (50%) were normal, regardless of the patient’s mutation or disease status (Fig. 3D and E) (some data not shown). DISCUSSION ...
Extensive post-transcriptional regulation of miRNAs within
... Imperial College London, London, United Kingdom ...
... Imperial College London, London, United Kingdom ...
Metagenomics: DNA sequencing of environmental samples
... pathogens and symbionts, which cannot survive outside their hosts; environmental microbes, most of which cannot be grown in pure culture; and ancient organisms whose only record is fossilized remains. DNA for these studies is extracted directly from the organisms in their natural habitat, such as ho ...
... pathogens and symbionts, which cannot survive outside their hosts; environmental microbes, most of which cannot be grown in pure culture; and ancient organisms whose only record is fossilized remains. DNA for these studies is extracted directly from the organisms in their natural habitat, such as ho ...
Crystal structure of the nucleosome core particle at 2.8 Å
... DNA of defined sequence and histones lacking post-translational modifications (22,23), diffract anisotropically to between 1.8-2.2 Å. Recombinant histone proteins enabled heavy-atom derivatives to be prepared through the substitution of cysteine at many sites. Use of an ESRF undulator beam line allo ...
... DNA of defined sequence and histones lacking post-translational modifications (22,23), diffract anisotropically to between 1.8-2.2 Å. Recombinant histone proteins enabled heavy-atom derivatives to be prepared through the substitution of cysteine at many sites. Use of an ESRF undulator beam line allo ...
Lecture Handouts
... As you read these pieces bear in mind that Lewontin and Pinker have been on opposite sides of the debate over biological determinism for many years. Lewontin has often been labelled as a Marxist critic of biological determinism while Pinker is often interpreted as a strong biological reductionist. S ...
... As you read these pieces bear in mind that Lewontin and Pinker have been on opposite sides of the debate over biological determinism for many years. Lewontin has often been labelled as a Marxist critic of biological determinism while Pinker is often interpreted as a strong biological reductionist. S ...
CRISPR: The Last Piece of the Genetic Puzzle
... unwanted and potentially harmful mutation. Another problem is delivering the Cas9 protein and the guide crRNA into the cells.14 However, CRISPR/Cas9 is a new technology, and new systems are being developed to overcome these challenges. Synthetic CRISPR RNA (scrRNA) uses chemically modified nucleotid ...
... unwanted and potentially harmful mutation. Another problem is delivering the Cas9 protein and the guide crRNA into the cells.14 However, CRISPR/Cas9 is a new technology, and new systems are being developed to overcome these challenges. Synthetic CRISPR RNA (scrRNA) uses chemically modified nucleotid ...
Eliminate unnecessary lanes in gels
... In order to determine whether the transposome had really inserted at random into the genome, we examined the sequences in the genome into which the transposons had inserted. To do this, we first recovered each of the transposons along with a portion of the adjacent genomic DNA via a plasmid rescue p ...
... In order to determine whether the transposome had really inserted at random into the genome, we examined the sequences in the genome into which the transposons had inserted. To do this, we first recovered each of the transposons along with a portion of the adjacent genomic DNA via a plasmid rescue p ...
Expression effects
... Epigenetics – Genomic imprinting Modification of specific genes during gametogenesis so that only the paternal or maternal allele is expressed after fertilization, i.e. “Parent of origin” gene expression A limited number of genes behave in this way • ~80 of 30,000 human genes • Primarily expressed ...
... Epigenetics – Genomic imprinting Modification of specific genes during gametogenesis so that only the paternal or maternal allele is expressed after fertilization, i.e. “Parent of origin” gene expression A limited number of genes behave in this way • ~80 of 30,000 human genes • Primarily expressed ...
2014-2015 Internship descriptions
... simultaneously with histone modifications and chromatin associated proteins. Using confocal microscopy we then record high resolution 3D images of nuclei and analyze colocalization of several signals. The internship will be adapted to fit with the current research carried out in our group. Right now ...
... simultaneously with histone modifications and chromatin associated proteins. Using confocal microscopy we then record high resolution 3D images of nuclei and analyze colocalization of several signals. The internship will be adapted to fit with the current research carried out in our group. Right now ...
NULL ALLELES OF HUMAN COMPLEMENT C4 Evidence for
... C4 is the only component of the human complement system that is coded for by two genes, C4A and C4B, showing 99% homology. The genes for the two C4 isotypes are located with the genes for the second component (C2), factor B (BF), and steroid 21-hydroxylase (21-OHA and 21-OHB) between HLA-B and -DR i ...
... C4 is the only component of the human complement system that is coded for by two genes, C4A and C4B, showing 99% homology. The genes for the two C4 isotypes are located with the genes for the second component (C2), factor B (BF), and steroid 21-hydroxylase (21-OHA and 21-OHB) between HLA-B and -DR i ...
Salma Nassef
... Hereditary breast and ovarian cancer (HBOC) is an inherited cancer syndrome that is associated with mutations in the BRCA1 and BRCA2 genes. Carriers of BRCA mutations, both men and women, are at an increased risk for developing certain cancers. Carriers are most notably at an increased risk to devel ...
... Hereditary breast and ovarian cancer (HBOC) is an inherited cancer syndrome that is associated with mutations in the BRCA1 and BRCA2 genes. Carriers of BRCA mutations, both men and women, are at an increased risk for developing certain cancers. Carriers are most notably at an increased risk to devel ...
MCB 421-2006: Homologous Recombination
... effect (60% decrease), — the two genes must be working in separate pathways, and there are more functional pathways left; 3) the double mutant shows a synergistic effect (99% down) — there are only two pathways, and the two mutations inactivate both. To see how epistatic analysis works, let us cons ...
... effect (60% decrease), — the two genes must be working in separate pathways, and there are more functional pathways left; 3) the double mutant shows a synergistic effect (99% down) — there are only two pathways, and the two mutations inactivate both. To see how epistatic analysis works, let us cons ...
HNF-1B specifically regulates the transcription of the
... Since deletion of the entire HNF1B gene is frequently found in human patients, it seems likely that a gene dosage effect is involved. However, some mutated factors behave as dominant negative proteins that may possibly inactivate the wild type protein [9]. Recently, novel mutations in the HNF1B gene ...
... Since deletion of the entire HNF1B gene is frequently found in human patients, it seems likely that a gene dosage effect is involved. However, some mutated factors behave as dominant negative proteins that may possibly inactivate the wild type protein [9]. Recently, novel mutations in the HNF1B gene ...
Here - EdSpace
... to modify any genomic sequence with high levels of specificity. The system first found in bacteria allows these species to develop resistance to foreign genetic elements, providing an acquired immunity.1 More recently, the technique has been expanded to model organisms, such as C. elegans, in which ...
... to modify any genomic sequence with high levels of specificity. The system first found in bacteria allows these species to develop resistance to foreign genetic elements, providing an acquired immunity.1 More recently, the technique has been expanded to model organisms, such as C. elegans, in which ...
Cancer epigenetics
Cancer epigenetics is the study of epigenetic modifications to the genome of cancer cells that do not involve a change in the nucleotide sequence. Epigenetic alterations are as important as genetic mutations in a cell’s transformation to cancer, and their manipulation holds great promise for cancer prevention, detection, and therapy. In different types of cancer, a variety of epigenetic mechanisms can be perturbed, such as silencing of tumor suppressor genes and activation of oncogenes by altered CpG island methylation patterns, histone modifications, and dysregulation of DNA binding proteins. Several medications which have epigenetic impact are now used in several of these diseases.