Document
... The enzyme RNA polymerase binds to a site on the DNA at the start of a gene (The sequence of DNA that is transcribed into RNA is called a gene). RNA polymerase separates the DNA strands and synthesises a complementary RNA copy from the antisense DNA strand It does this by covalently bonding ribonucl ...
... The enzyme RNA polymerase binds to a site on the DNA at the start of a gene (The sequence of DNA that is transcribed into RNA is called a gene). RNA polymerase separates the DNA strands and synthesises a complementary RNA copy from the antisense DNA strand It does this by covalently bonding ribonucl ...
2.7 DNA replication, transcription and translation
... The enzyme RNA polymerase binds to a site on the DNA at the start of a gene (The sequence of DNA that is transcribed into RNA is called a gene). RNA polymerase separates the DNA strands and synthesises a complementary RNA copy from the antisense DNA strand It does this by covalently bonding ribonucl ...
... The enzyme RNA polymerase binds to a site on the DNA at the start of a gene (The sequence of DNA that is transcribed into RNA is called a gene). RNA polymerase separates the DNA strands and synthesises a complementary RNA copy from the antisense DNA strand It does this by covalently bonding ribonucl ...
New genes with old modus operandi
... genes. For example, null mutations in the mukB gene caused conditional lethality associated with filamentous cells and irregular nucleoid distribution. Thus, not only the positioning but also the separation of the nucleoids was affected. Nucleoids in cells of a ΔmukB strain had lost their position a ...
... genes. For example, null mutations in the mukB gene caused conditional lethality associated with filamentous cells and irregular nucleoid distribution. Thus, not only the positioning but also the separation of the nucleoids was affected. Nucleoids in cells of a ΔmukB strain had lost their position a ...
telomeres - Atlas of Genetics and Cytogenetics in Oncology and
... VI.3.1. Molecular and cytogenetic studies have indicated chromosomes with even a single unprotected chromosome end are genetically unstable until telomere integrity has been restored. During this period of genetic instability, breakage-fusion-breakage (BFB) cycles occur, often culminating in chromos ...
... VI.3.1. Molecular and cytogenetic studies have indicated chromosomes with even a single unprotected chromosome end are genetically unstable until telomere integrity has been restored. During this period of genetic instability, breakage-fusion-breakage (BFB) cycles occur, often culminating in chromos ...
Gel electrophoresis of restriction digest
... runs. Both Xylene Cyanol and Bromphenol Blue contain glycerol which increases the density of the sample for easier loading. One the gel electrophoresis has been completed ethidium bromide us used for the direct visualization of DNA in gels. The dye intercalates between the stacked bases on nucleic a ...
... runs. Both Xylene Cyanol and Bromphenol Blue contain glycerol which increases the density of the sample for easier loading. One the gel electrophoresis has been completed ethidium bromide us used for the direct visualization of DNA in gels. The dye intercalates between the stacked bases on nucleic a ...
What is a Mutation?
... The diagram shows three generations of cells produced by a single cell through mitosis. In the process, a single mutation occurred at the point indicated. The mutation caused changes within a dominant allele. How many of the 15 cells contain the mutation? Apr'06 10th -20 ...
... The diagram shows three generations of cells produced by a single cell through mitosis. In the process, a single mutation occurred at the point indicated. The mutation caused changes within a dominant allele. How many of the 15 cells contain the mutation? Apr'06 10th -20 ...
This article appeared in a journal published by Elsevier. The
... by these DNA vaccines through intramuscular immunization. The IgG subclass (IgG1 and IgG2a) analysis indicates that divalent DNA vaccine induces both Th1 and Th2 immune responses. The protective efficacy was evaluated by the survival against lethal challenge with A. pleuropneumoniae serotype 1. The g ...
... by these DNA vaccines through intramuscular immunization. The IgG subclass (IgG1 and IgG2a) analysis indicates that divalent DNA vaccine induces both Th1 and Th2 immune responses. The protective efficacy was evaluated by the survival against lethal challenge with A. pleuropneumoniae serotype 1. The g ...
Introduction to the course II
... haploid MATa cell, all cells in the colony will be MATa (haploid). Similarly, it is also possible to grow colonies of MATα (haploid) or MATa/MATα (diploid) cells. Homothalic strains exist only as MATa/MATα. This is due to the fact that the haploid homothallic cells change their mating type (!) durin ...
... haploid MATa cell, all cells in the colony will be MATa (haploid). Similarly, it is also possible to grow colonies of MATα (haploid) or MATa/MATα (diploid) cells. Homothalic strains exist only as MATa/MATα. This is due to the fact that the haploid homothallic cells change their mating type (!) durin ...
Article PDF
... was observed between the first 50 ns and the rest of the trajectory. During the first 50 ns, the first two peaks of the nucleosome-sodium RDF showed a subtle shift of some sodium from the second peak to the first. We did not observe any significant changes in nucleosomeion RDFs after the first 50 ns ...
... was observed between the first 50 ns and the rest of the trajectory. During the first 50 ns, the first two peaks of the nucleosome-sodium RDF showed a subtle shift of some sodium from the second peak to the first. We did not observe any significant changes in nucleosomeion RDFs after the first 50 ns ...
Gene expression regulation and the lactase gene
... Fig. 22. Structure and biosynthesis of the lactase protein. Biosynthesis of the lactase protein. In differentiated small intestinal enterocytes the 49.3 kb lactase gene is transcribed into a 6.2 kb mRNA (step a). Translation of lactase mRNA results in the pre-pro-LPH which is translocated over the e ...
... Fig. 22. Structure and biosynthesis of the lactase protein. Biosynthesis of the lactase protein. In differentiated small intestinal enterocytes the 49.3 kb lactase gene is transcribed into a 6.2 kb mRNA (step a). Translation of lactase mRNA results in the pre-pro-LPH which is translocated over the e ...
How functions are combined within the ABC model
... APETALA2 is a class A gene required for whorl 1 and 2, but is expressed in all whorls. How is AP2 activity restricted to whorls 1 and 2 ? ...
... APETALA2 is a class A gene required for whorl 1 and 2, but is expressed in all whorls. How is AP2 activity restricted to whorls 1 and 2 ? ...
Physicochemical studies on interactions between DNA and RNA
... The interaction between Escherichia coli RNA polymerase and a restriction fragment of coliphage T7 DNA containing four promoter sites for the coli enzyme has been studied by difference uv absorption spectroscopy in a low ionic strength buffer containing 10 mM MgCl 2 and 50 mM KC1. The binding of the ...
... The interaction between Escherichia coli RNA polymerase and a restriction fragment of coliphage T7 DNA containing four promoter sites for the coli enzyme has been studied by difference uv absorption spectroscopy in a low ionic strength buffer containing 10 mM MgCl 2 and 50 mM KC1. The binding of the ...
mutations
... Any _change_ in DNA _sequence_ is called a _mutation_. Can be _caused_ by errors in _replication_, _transcription_, cell _division_, or by _external_ agents. If _mutation_ occurs in _gametes_ (sex cells) it will be __passed_ on to _offspring_. May _produce_ a new __trait_ or it may result in ...
... Any _change_ in DNA _sequence_ is called a _mutation_. Can be _caused_ by errors in _replication_, _transcription_, cell _division_, or by _external_ agents. If _mutation_ occurs in _gametes_ (sex cells) it will be __passed_ on to _offspring_. May _produce_ a new __trait_ or it may result in ...
... heritable during division, but appear unrelated to DNA sequence changes, and can be modified by environmental stimuli [4, 5]. In a more recent view, epigenetics encompasses “mitotically heritable alterations in gene expression potential” [6], a definition that we have favored in this paper. Epigenet ...
Things to know for the Final - Mercer Island School District
... Be able to define photosynthesis and know the reactants and products for this process. Be able to describe an ATP molecule (adenosine triphosphate), including which part of this molecule contains a high energy bond. Be able to explain the ATP/ADP cycle that is continually occurring in cells. Include ...
... Be able to define photosynthesis and know the reactants and products for this process. Be able to describe an ATP molecule (adenosine triphosphate), including which part of this molecule contains a high energy bond. Be able to explain the ATP/ADP cycle that is continually occurring in cells. Include ...
Cancer, Genes, Inheritance Pattern, Germ Cell Mutation
... Environmental factors One example of an environmental factor that can damage our DNA is sun exposure. Other environmental factors include exposure to various toxins and radiation, lifestyle and diet. ...
... Environmental factors One example of an environmental factor that can damage our DNA is sun exposure. Other environmental factors include exposure to various toxins and radiation, lifestyle and diet. ...
FAQs about experiments that are exempt from NIH Guidelines
... The deliberate transfer of a drug resistance trait to microorganisms that are not known to acquire the trait naturally, if such acquisition could compromise the use of the drug to control disease agents in humans, veterinary medicine or agriculture [Section III-A]; ...
... The deliberate transfer of a drug resistance trait to microorganisms that are not known to acquire the trait naturally, if such acquisition could compromise the use of the drug to control disease agents in humans, veterinary medicine or agriculture [Section III-A]; ...
The global repressor FliZ antagonizes gene
... analogous question, how RNAP holoenzymes containing either s70 or sS themselves discriminate between these promoters, has puzzled scientists for years. Only recently was it understood that modular combinations of certain small promoter features can generate preferred recognition and/or activation of ...
... analogous question, how RNAP holoenzymes containing either s70 or sS themselves discriminate between these promoters, has puzzled scientists for years. Only recently was it understood that modular combinations of certain small promoter features can generate preferred recognition and/or activation of ...
brief talk
... Lauria, Mario, Kaustubh Bhalerao, Muthu M. Pugalanthiran, and Bo Yuan. “Building blocks of a biochemical CPU based on DNA transcription logic.” 3rd Workshop on Non-Silicon Computation (NSC-3), Munich, June 2004. Molecular Beacons: A Novel DNA Probe for Nucleic Acid and Protein ...
... Lauria, Mario, Kaustubh Bhalerao, Muthu M. Pugalanthiran, and Bo Yuan. “Building blocks of a biochemical CPU based on DNA transcription logic.” 3rd Workshop on Non-Silicon Computation (NSC-3), Munich, June 2004. Molecular Beacons: A Novel DNA Probe for Nucleic Acid and Protein ...
Identification of three MADS‐box genes expressed in sunflower
... As a ®rst step towards this goal, MADS-box containing cDNAs were cloned from sun¯ower R2-stage in¯orescence RNA by RTPCR using degenerated oligonucleotides deduced from conserved regions of members of the AG and DEF groups. Only one expressed gene could be identi®ed with oligonucleotides for the AG ...
... As a ®rst step towards this goal, MADS-box containing cDNAs were cloned from sun¯ower R2-stage in¯orescence RNA by RTPCR using degenerated oligonucleotides deduced from conserved regions of members of the AG and DEF groups. Only one expressed gene could be identi®ed with oligonucleotides for the AG ...
Student`s guide -
... Loading the gel a. Pour slightly more than 10 mL of TBE buffer solution into the gel tank. The liquid should just cover the surface of the gel and flood into the areas at either end. b. Very gently ease the comb from the gel, allowing the buffer solution to fill the wells left behind. Take care no ...
... Loading the gel a. Pour slightly more than 10 mL of TBE buffer solution into the gel tank. The liquid should just cover the surface of the gel and flood into the areas at either end. b. Very gently ease the comb from the gel, allowing the buffer solution to fill the wells left behind. Take care no ...
Regulatory sequences
... Scaffold/matrix attached regions (S/MARs) are regions of the DNA strand that are found the basis of chromatin loops. They anchor the DNA to the proteinaceous nuclear matrix. Each loop is considered to be a functional domain. S/MARs genes residual DNA ...
... Scaffold/matrix attached regions (S/MARs) are regions of the DNA strand that are found the basis of chromatin loops. They anchor the DNA to the proteinaceous nuclear matrix. Each loop is considered to be a functional domain. S/MARs genes residual DNA ...
Cancer epigenetics
Cancer epigenetics is the study of epigenetic modifications to the genome of cancer cells that do not involve a change in the nucleotide sequence. Epigenetic alterations are as important as genetic mutations in a cell’s transformation to cancer, and their manipulation holds great promise for cancer prevention, detection, and therapy. In different types of cancer, a variety of epigenetic mechanisms can be perturbed, such as silencing of tumor suppressor genes and activation of oncogenes by altered CpG island methylation patterns, histone modifications, and dysregulation of DNA binding proteins. Several medications which have epigenetic impact are now used in several of these diseases.