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DNA and RNA
DNA and RNA

... substance such as a chemical that causes mutations mutation permanent change in a cell’s DNA ranging from changes in a single base pair to deletions of large sections of chromosomes Hox genes A form of gene regulation in an eukaryotic embryo where a series of genes controls the organs and tissues th ...
GENETICS The Future of Medicine
GENETICS The Future of Medicine

... patients facing chemotherapy may experience fewer side effects and improve their prognoses by first getting a genetic fingerprint of their tumor. This fingerprint can reveal which chemotherapy choices are most likely to be effective. Better understanding of genetics promises a future of precise, cus ...
Essential Question
Essential Question

... What is the complimentary sequence: A A T G C A TTA CGT 8. The two sides of DNA are held together by Hydrogen _______ bonds. 9. DNA is composed of repeating subunits called nucleotides ______________________ 10. What are the 4 bases that make up the rungs of the DNA Adenine Thymine Guanine Cytosine ...
Document
Document

... 3. Gel electrophoresis separates DNA on the basis of size. 4. DNAs can be synthesized (up to ~100 bases commercially). (N) 5. PCR amplifies any target DNA sequence. (N) 6. Genes and genomes can be sequenced by chain termination. (N) 7. Oligonucleotides can be used to change bases by “site- directed ...
Test Review on DNA Structure, DNA Replication
Test Review on DNA Structure, DNA Replication

... Understand that DNA is a double helix composed of two strands of nucleotides and be able to describe this shape. Know the three parts of a nucleotide. Understand that the nitrogen base is the part of the nucleotide that forms the genetic code, and be able to name the four possible nitrogen bases in ...
Human Mitochondrial DNA
Human Mitochondrial DNA

... Transformation – the uptake and expression of foreign DNA by a cell Transduction – the use of viruses to transform or genetically engineer cells Competent/competency – the ability of cells to take up DNA Selection – the process of screening potential clones for the expression of a particular gene, f ...
12-5 Gene Regulation - Lincoln Park High School
12-5 Gene Regulation - Lincoln Park High School

... Operator- area of a chromosome in an operon that the repressor binds to when the operon is “turned off” Lac genes are turned off by the repressor Lac genes are turned on by the presence of lactose ...
Chapter 3,
Chapter 3,

Yeast Saccharomyces cerevisiae
Yeast Saccharomyces cerevisiae

... suitable for genetic manipulation - first eukaryotic organism stabily transformed with exogenous nonreplicative DNA, by integration into the genome, via homologous recombination (Hinnen et al., 1978) ...
Practicing Protein Synthesis
Practicing Protein Synthesis

Gel Electophoresis: Forensic Plasmid DNA identification
Gel Electophoresis: Forensic Plasmid DNA identification

Answers chapter 9
Answers chapter 9

... selection acts upon. Mutations therefore permit the appearance of new traits that can prove successful in a particular environment or allow groups to adapt to a changing one. While the rate at which new mutations arise partly reflects uncontrolled environmental factors such as exposure to mutagenic ...
Document
Document

... Once the vector is isolated in large quantities, it can be introduced into the desired host cells such as mammalian, yeast, or special bacterial cells. The host cells will then synthesize the foreign protein from the recombinant DNA. When the cells are grown in vast quantities, the foreign or recomb ...
Lecture 29 (4-15-11)
Lecture 29 (4-15-11)

... Control of eyespot development on a developing butterfly wing ...
Biol518Lec2final-2 - Cal State LA
Biol518Lec2final-2 - Cal State LA

... sequence homology (denoted by the solid box) to orfX (denoted by the wide arrow). When the plasmid is introduced into wild-type cells (W+), a single cross-over recombination event between these two regions of homology leads to insertion of plasmid sequences and disruption of the orfX reading frame. ...
Several Features Distinguish Eukaryotic Processes From
Several Features Distinguish Eukaryotic Processes From

... associated with decreased gene expression; corepressors often possess histone deacetylation ...
Bio 309F
Bio 309F

Transcription – Part II
Transcription – Part II

... 8. Regulation of gene expression in eukaryotes is considered much more complex than in prokaryotes. Why do you think that is? 9. What is the role of enhancers and silencers in transcriptional regulation? 10. Describe the three different DNA binding motifs associated with transcription factors. 11. U ...
Materials and Methods
Materials and Methods

... Microarray Suite (MAS) version 5.0 (Affymetrix) and linearly scaled to achieve an average intensity of 150 across each chip. The candidate gene list obtained from MAS 5.0-extracted data was selected by eliminating genes that were not present in both experiments. The arrays were subjected to a pair w ...
Secondary structure of RNA
Secondary structure of RNA

... autoimmune diseases, the body harms its own healthy cells and tissues. This leads to inflammation and damage of various body tissues. Lupus can affect many parts of the body, including the joints, skin, kidneys, heart, lungs, blood vessels, and brain. Although people with the disease may have many d ...
Name _________KEY___________________________
Name _________KEY___________________________

... 33. What is a DNA fingerprint? The pattern of bands that results when an individual’s DNA fragments are separated 34. What is Polymerase Chain Reaction (PCR)? A process used to make many copies of selected segments of DNA. This is often needed when only a small sample is available for analysis (i.e. ...
DNA Replication
DNA Replication

... that are identical (except for rare mutations). The two identical daughter chromosomes move toward opposite end of the cell. When the cell divides the daughter chromosomes are partitioned one to each daughter cell. ...
Contract No: FIGH-CT-1999-00006
Contract No: FIGH-CT-1999-00006

... Ptch+/- mouse strain adds further weight to the view that such rare mutations can substantially increase radiation cancer risk albeit with the potential for modification by other genes. The maximum enhancement of cancer risk at young ages of irradiation is an important issue and requires further fol ...
Document
Document

... Biosystems, use 4 colors, so they can read all 4 bases at once. ...
Slide 1
Slide 1

... However, some will be beneficial, and the cell with the new genetic information resulting from the mutation will be able to outperform other cells. This enhanced fitness at the cellular level may increase the survival and reproductive performance of the organism, and in that case the mutation will b ...
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Cancer epigenetics



Cancer epigenetics is the study of epigenetic modifications to the genome of cancer cells that do not involve a change in the nucleotide sequence. Epigenetic alterations are as important as genetic mutations in a cell’s transformation to cancer, and their manipulation holds great promise for cancer prevention, detection, and therapy. In different types of cancer, a variety of epigenetic mechanisms can be perturbed, such as silencing of tumor suppressor genes and activation of oncogenes by altered CpG island methylation patterns, histone modifications, and dysregulation of DNA binding proteins. Several medications which have epigenetic impact are now used in several of these diseases.
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