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... arrest from G1 and don’t undergo apoptosis. 2. Telomerase enzyme is activated, avoiding the limit to cell divisions imposed by telomere shortening. ...
Biol 3301: Genetics Exam #3 Practice questions
Biol 3301: Genetics Exam #3 Practice questions

... By determining which strand is methylated. The old (template) strand will be methylated. 11. (3) Once damaged bases are removed by ___DNA glycosylases____________, the resulting AP sites are repaired by ____base excision repair/AP repair__________. 12. (3) Name two reasons why heterozygous deletions ...
A trait - Images
A trait - Images

... Full-shaded circle represents a female with the trait Full-shaded square represents a male with the trait ...
Gene discovery within the planctomycete division of the domain
Gene discovery within the planctomycete division of the domain

... that may have appeared in the planctomycetes via horizontal gene transfer events. Significant among these matches are those with two genes atypical for Bacteria and with significant cellbiology implications - integrin alpha-V and inter-alpha-trypsin inhibitor protein - with homologs in G. obscuriglo ...
Section J Analysis and Uses of Cloned DNA
Section J Analysis and Uses of Cloned DNA

... • The two main methods of DNA sequencing are (1) Maxam’s method: the end-labeled DNA is subjected to base-specific cleavage reactions prior to gel separation; (2) Sanger’s method: to uses di-dNTP as chain terminators to produce a ladder of molecules generated by polymerase extension of a primer. • A ...
Great Discoveries in Science: The Double Helix [JUDSON:] In the
Great Discoveries in Science: The Double Helix [JUDSON:] In the

... everything that could prove relevant for their ongoing-- but now underground-- quest to discover the structure of DNA. [WATSON:] To me there was only one way I could be happy... or two ways, you know: solve DNA or get a girlfriend... [laughs] and I didn't get a girlfriend, so it was solve DNA. [JUDS ...
The Cell Biological Basis of Cancer - thesis
The Cell Biological Basis of Cancer - thesis

... damage to cellular DNA can be a continual and process caused by a variety of mutagens (Loeb, Loeb, & Anderson, 2003). Reactive metabolites and certain environmental species are known to induce an alteration in the structure of DNA, and these chemical alterations of the genomic material can lead to m ...
Heredity and Genetics
Heredity and Genetics

Heredity and Genetics PowerPoint
Heredity and Genetics PowerPoint

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Hailey Spelman - Determining Cellular Fate: Pre- and Postnatal Methylation Effects on Gene Expression
Hailey Spelman - Determining Cellular Fate: Pre- and Postnatal Methylation Effects on Gene Expression

... relative to the strength of the promoter— that occurs in these areas, the greater the chance that low or no transcription will occur (Phillips). This repression is greater if the promoter itself is methylated, but distantly methylated sequences can also contribute to repression —especially if the me ...
Research Fast Facts: BRCA
Research Fast Facts: BRCA

... Komen-funded research has helped us to understand that: ...
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Prok and Euk Gene Expression

Tuesday 4/8/14
Tuesday 4/8/14

... • Copies DNA • used to make many copies of a gene after isolation • Allows the analysis of short pieces of DNA or RNA without having to clone it ...
Genetic Testing for Cancer Susceptibility
Genetic Testing for Cancer Susceptibility

... Individual has breast cancer with 2 or more first, second or third degree relatives (related through a single lineage) with breast or epithelial ovarian / fallopian tube / primary peritoneal cancer Individual has relatives with documented mutations in either the BRCA1 or BRCA2 gene Individual has fa ...
Topic 3 notesTEACHER
Topic 3 notesTEACHER

... body cell— (soma cell) any cell in the body that is not a sex cell Bond--a mutual attraction between two atoms Centriole—one of two tiny structures in an animal cell that helps form spindle fibers Centromere—connection between two homologous chromosomes Cytokinesis—division of the cytoplasm Chromoso ...
Chapter 21: Molecular Basis of Cancer
Chapter 21: Molecular Basis of Cancer

Biotechnology Laboratory
Biotechnology Laboratory

... 5. Biofuels connections, RT-qPCR analysis of MEP pathway gene expression. Objective: To investigate the expression levels of selected 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway genes in the cyanobacterium Synechococcus sp. PCC 7002. As mentioned, we have introduced IspS and IDI genes into thi ...
Identically Different: Why You Can Change Your Genes
Identically Different: Why You Can Change Your Genes

... who sadly died during the operation meant to separate them. Despite being joined at the head, and thus spending their entire lives together, each had different interests, abilities, and aspirations. Spector asks ‘if our genes and environment are the same, how can there be any room for differences be ...
What Do You Mean, “Epigenetic”?
What Do You Mean, “Epigenetic”?

... 2010). Those in the field of genetics concerned with DNA methylation, chromatin activity states, chromosomal imprinting, centromere function, etc., predominantly use Holliday’s notion of epigenetics. They are interested in how expression patterns persist across different cells (mitosis) and generatio ...
Recombinant DNA
Recombinant DNA

... Project revealed that most of the human genome does not consist of genes  Results of the Human Genome Project – Humans have 21,000 genes in 3.2 billion nucleotide pairs – Only 1.5% of the DNA codes for proteins, tRNAs, or rRNAs – The remaining 88.5% of the DNA contains – Control regions such as pro ...
Yeast DNA Prep (Quick) Formosa
Yeast DNA Prep (Quick) Formosa

... appropriate temperature (usually 30°). If recovering a plasmid that can be lost, grow the strain on a plate to select for retention of the plasmid, then scrape up a large glob of cells for the inoculation; this works better than using selective liquid medium since the yield of cells is 5-10 X higher ...
principles of genetics
principles of genetics

... molecule in terms of: the components of DNA nucleotides; the sugar-phosphate backbone; specific base pairing and hydrogen bonding between polynucleotide strands (only simple diagrams of DNA structure are needed; structural formulae are not required). Explain how the . structure of DNA is related to ...
14MG-Website-Content-Draft-v2-2014-11-10
14MG-Website-Content-Draft-v2-2014-11-10

Mutations and Evolution
Mutations and Evolution

... mRNA. In RNA the nucleic acid base uracil (U) replaces the thymine in DNA, so investigations have found that many uracil pairs with the adenine in DNA during copying. Transfer RNA (tRNA) molecules of the ‘frequent mutations’ in the attached to each of the amino acids each have complementary triplet ...
Detection of unpaired DNA at meiosis results in RNA‐mediated
Detection of unpaired DNA at meiosis results in RNA‐mediated

... arrested at meiotic prophase. This phenotype allowed mapping to linkage group I and cloning of sad-1þ by complementation.(2) It also has provided a more efficient method for the isolation of further Sad-1 mutations. A complete sad-1 deletion yielded almost 100% dominance while a series of sad-1 alle ...
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Cancer epigenetics



Cancer epigenetics is the study of epigenetic modifications to the genome of cancer cells that do not involve a change in the nucleotide sequence. Epigenetic alterations are as important as genetic mutations in a cell’s transformation to cancer, and their manipulation holds great promise for cancer prevention, detection, and therapy. In different types of cancer, a variety of epigenetic mechanisms can be perturbed, such as silencing of tumor suppressor genes and activation of oncogenes by altered CpG island methylation patterns, histone modifications, and dysregulation of DNA binding proteins. Several medications which have epigenetic impact are now used in several of these diseases.
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