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DNA Sequencing
DNA Sequencing

... 13.4 Finding a Specific Clone in a Library ...
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Nucleus

... building of one DNA strand based on the bases in 2nd strand • Steps of replication process – DNA helicase opens short segment of helix • replication fork is point of separation of 2 strands ...
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... • 1970 : Arber and Meselson Arber and Meselson discovered type I restriction enzymes which cleave DNA randomly away from the recognition site. • Also In 1970, Smith, Kelly and Welcox isolated and characterized the first type II restriction enzyme, HindII, from the bacterium Haemophilus influenzae t ...
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Nucleus - Maryville University

... building of one DNA strand based on the bases in 2nd strand • Steps of replication process – DNA helicase opens short segment of helix • replication fork is point of separation of 2 strands ...
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Genetics (4) - HCC Learning Web

... building of one DNA strand based on the bases in 2nd strand • Steps of replication process – DNA helicase opens short segment of helix • replication fork is point of separation of 2 strands ...
DNA Fingerprinting: The Code to Identification
DNA Fingerprinting: The Code to Identification

... out to be members of the royal family, the Romanovs, who had been executed in 1918. Because the Y chromosome, part of the nuclear genome, is passed largely intact from father to son for many generations, DNA fingerprinting of the Y chromosome can be used to trace male lineages. Human DNA fingerprint ...
CHEM F450
CHEM F450

... An account with Sapling Learning must be set up in order to complete mandatory homework assignments. To do this: 1. Go to http://saplinglearning.com and click on your "US Higher Ed" at the top right. 2a. If you already have a Sapling account, log in and skip to step 3. 2b. If you have Facebook accou ...
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... B. the repressor binds to tryptophan and then leaves the operator C. tryptophan binds to the operator and prevents transcription D. tryptophan binds to the repressor, which than binds to the operator E. tryptophan binds to the repressor, which binds to the promoter and prevents transcription ____ 19 ...
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... health, and health promotion and education activities to promote public health EPA: The federal agency that enforces environmental laws including the production and use of microorganisms, herbicides, pesticides, and GMOs USDA: Federal agency that regulates the use and production of plants, plant pro ...
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... • besides, sequencing the rest, often called “junk DNA”, – “...(it) would be a waste of time and money to include the repetitive, hard-to-sequence regions in the genome project.” ...
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DNA Technology and Genomes

... Improved techniques have made it possible to find genomes of other organisms Provided new insights into role of “junk DNA” (sections of DNA that are not transcribed) Found far fewer protein coding regions than expected ...
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... • besides, sequencing the rest, often called “junk DNA”, – “...(it) would be a waste of time and money to include the repetitive, hard-to-sequence regions in the genome project.” ...
Zoo/Bot 3333
Zoo/Bot 3333

... Samples of DNA obtained from a fetus (F) and her parents (M and P) were cut by restriction enzyme R, then analyzed by gel electrophoresis followed by the Southern blot technique and hybridization with the radioactively labeled DNA probe designated “CF probe” in the above figure. Enzyme R has a six b ...
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... fat cat. – The rat cat. • Nonsense mutations are point mutations that create a premature "translation stop signal" (or "stop" codon), causing the protein to be shortened. UAC – UAG. The fat cat. – the cat cat. • Silent mutations are point mutations that do not cause amino acid changes within the pro ...
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... Analyze genetic variation among humans • The genome is approximately 99.9% identical between individuals of all nationalities and backgrounds. ...
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... fluorescence from different dye terminators that are used to identify the A C G T bases. Each dye emits light at a different wavelength when excited by an argon ion laser. All four bases are then detected & distinguished in a single capillary. • Sequencing involves creating an electrical flow of ion ...
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Lecture#22 - Cloning DNA and the construction of clone libraries

...  - each is transformed into a different host cell  - get a set of clones -> a library. In the test tube -> many (106 -109) molecules -> many molecules joining together which transform many cells. At the end -> many clones 10's, 100's, 1000's etc. **** This library would have many duplicate cloned ...
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... 4. DNA synthesis always proceeds in a 5’ 3’ direction. 5. DNA polymerases catalyze the linking together of the nucleotide subunits. 6. Nucleotides with three phosphate groups are used as substrates for the polymerization reaction. Two of the phosphates are removed and the nucleotide is added to the ...
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... in the DNA strand where they interrupt the coding sequence of another gene, thus causing an incorrect protein or no protein to be formed. These are commonly known as “jumping genes”, and can even jump across species, such as from bacteria to plants! 2. Genetic Recombination – bacteria can transfer g ...
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... a. a collection of cloned DNA of the same gene loci from different species b. a library of putative genes including promoters and operators c. a type of genomic library containing an organism's complete genome. d. a DNA library made up of DNA clones reconstructed from mRNA fragments e. is composed o ...
2421_Ch8.ppt
2421_Ch8.ppt

... The process repeats so that one amino acid is added at a time to the growing polypeptide (which is always anchored to a tRNA bound within the ribosome) The polypeptide continues to grow until the ribosome reaches a stop codon At the stop codon, the polypeptide chain is released from the last tRNA a ...
Transcription and Translation Exercise
Transcription and Translation Exercise

... 3. Describe the relationship between the gene sequence and the tRNA sequence. They are the same except RNA contains U’s while DNA contains T’s. ...
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CpG methylation analysis from targeted

... RainDance Technologies (RDT) has extended the capabilities of the RDT 1000 to target genomic regions of bisulfite converted DNA. This approach used in conjunction with highthroughput sequencing enables researchers to measure the methylation status of targeted regions of the genome with complete sequ ...
DNA and Genealogy
DNA and Genealogy

... a unit of recombinant frequency which is used to measure genetic distance. It is often used to imply distance along a chromosome, and takes into account how often recombination occurs in a region. A region with few cMs undergoes relatively less recombination. ...
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Epigenomics

Epigenomics is the study of the complete set of epigenetic modifications on the genetic material of a cell, known as the epigenome. The field is analogous to genomics and proteomics, which are the study of the genome and proteome of a cell (Russell 2010 p. 217 & 230). Epigenetic modifications are reversible modifications on a cell’s DNA or histones that affect gene expression without altering the DNA sequence (Russell 2010 p. 475). Two of the most characterized epigenetic modifications are DNA methylation and histone modification. Epigenetic modifications play an important role in gene expression and regulation, and are involved in numerous cellular processes such as in differentiation/development and tumorigenesis (Russell 2010 p. 597). The study of epigenetics on a global level has been made possible only recently through the adaptation of genomic high-throughput assays (Laird 2010) and.
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