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SEPA: Single-Cell Gene Expression Pattern Analysis
SEPA: Single-Cell Gene Expression Pattern Analysis

... ordering concept provides a novel method of exploring single-cell RNA-seq data. If one has available true experimental time or pseudo temporal cell ordering information, a natural question to ask is what expression patterns do these genes have along the true or pseudo time axis. The expression patte ...
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BOULANT Steeve PhD

... "Cell-dependent differences in virus uptake correlate with receptor abundance"  October 2010: ASBMB membrane traffic symposium. "Actin dynamics is essential to counteract membrane tension during clathrin-mediated endocytosis"  January 2010: EMBO membrane traffic workshop "Study of clathrin mediate ...
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ch_07_clicker_questions

... Which statement regarding prokaryotic chromosomes is incorrect? a) There are two copies of the chromosome so that cells are diploid. b) There is a single copy of the chromosome so that cells are haploid. c) Prokaryotic chromosomes are located in the nucleoid. d) Prokaryotic chromosomes are now know ...
DNA and Protein Synthesis WebQuest
DNA and Protein Synthesis WebQuest

... How does the mRNA leave the nucleus? ...
1 Cell Signaling Exam 1 Spring 2007. To avoid problems (such as
1 Cell Signaling Exam 1 Spring 2007. To avoid problems (such as

... groups. That is, the paired t Test is better than the pooled t test because abnormal values –high or low- weaken the power of the pooled t Test- there might be a significant difference but you would not see it” (c) 2 pts: paired t Test (same sample used for both control and experimental group). 4. 1 ...
Nature Rev.Genet
Nature Rev.Genet

... piRNAs are transcribed from W chromosome in females and reduces Masc mRNA levels from Marek, Nature 509, 570 (2014) Masc promotes male-specific splicing of doublesex ...
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NULL ALLELES OF HUMAN COMPLEMENT C4 Evidence for

... (Hybond N; Amersham Buehler), the blots were hybridized first with oligo A according to procedures described previously (29). After autoradiography for 6 h, the probe was removed by boiling the blots for 20 min in 0.1% SDS and rehybridized with oligo B. Oligos A and B were endlabeled with polynucleo ...
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The connection between transcription and genomic instability

... region more susceptible to attack by internal metabolites that are reactive with ssDNA, leading to both mutagenic and recombinogenic lesions. This hypothesis has the advantage that it can also explain TAM, even though more quantitative data are lacking on TAM to strengthen this possibility. In addit ...
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... b) based on a novel annotation of the fly genome. It contains 21376 gene- specific probes. ...
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Cloning, expression, and characterization of Fe

... Under normal growth conditions, cells of aerobic organisms produce low amounts of reactive oxygen species (ROS) but metabolic processes enhance the production under biotic and abiotic stress and the increased ROS concentrations affect many cellular functions by damaging nucleic acids, oxidizing prot ...
The principles and methods formulated by Gregor Mendel provide
The principles and methods formulated by Gregor Mendel provide

... of a gene have different alleles, the person is heterozygous. Often, in a heterozygous individual a dominant allele determines the observable characteristic and the other recessive allele does not affect the phenotype. Thus, a heterozygous person has the same phenotype as a person who is homozygous ...
and Flowers of Arabidopsis thaliana
and Flowers of Arabidopsis thaliana

... These simple sets of meristematic choices thus provide a tool kit for plant architecture, and the choice made at any time by each meristem sums to the total form of the plant. Little is known about the control of pattem formation in SAMs and their derivatives, but one thing is clear: It depends very ...
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Antimicrobial Resistance (no superbugs but dumb people

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... • Require host cell for replication • Consist of single short piece of RNA • No protective protein coat • Cause plant diseases • Some scientists speculate they may cause diseases in humans ...
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Bio Keystone Review

... A. Relationship between organisms’ structure and function B. Beneficial inherited trait that is passed to future generation C. Change in the genetic makeup of a population over time D. Change in biodiversity across Earth’s surface _______ 8. The variety of life across the biosphere is called A. biod ...
Analysis of a piwi-related Gene Implicates Small RNAs in
Analysis of a piwi-related Gene Implicates Small RNAs in

... 1.The next slide describes the enzymatic machineries that are likely to be involved in IES elimination by the scnRNA mechanism. 2. Evidence demonstrating the nature of the RNA polymerase and the RNAse III (dicer) were presented at the meeting. However, these data are unpublished and making this pre ...
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production and quality control of medicinal products derived

Chapter 11 Notes – Introduction to Genetics
Chapter 11 Notes – Introduction to Genetics

... 2. Codominance – both alleles are expressed; i.e. in certain varieties of chicken, the allele for black feathers is codominant with the allele for white feathers. Heterozygous chickens have a color described as “erminette,” speckled with black and white feathers. 3. Multiple Alleles – when genes hav ...
RECOMBINANT DNA TECHNOLOGY: APPLICATIONS IN THE
RECOMBINANT DNA TECHNOLOGY: APPLICATIONS IN THE

... fragment length polymorphisms (RFLPs), short tandem repeats (STRs) and single nucleotide polymorphisms (SNPs). All three can occur within genes as well as in intergenic regions, and altogether there are several million of these polymeric sites I the human genome, with SNPs being the most common. Gen ...
Beadle and Tatum 2
Beadle and Tatum 2

... The phenotypic appearance is therefore directly affected by gene expression. The extent of phenotypic differences depends on how different the DNA sequences are in individuals, but may also be influenced by the environment. To what extent differences in genotype cause differing phenotypes has always ...
Extracting DNA
Extracting DNA

... Sir Alec Jeffreys is credited with DNA profiling using RFLP. In September of 1984 after years of work, he saw his first series of blots on an X-ray. The technique was first used in forensics, when in 1985 he was asked by police to confirm the rape confession of 17 year old Richard Buckland, who was ...
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the genetic material

...  Replication Fork: a Y shaped point that ...
Chapter 11 Introduction to Genetics
Chapter 11 Introduction to Genetics

... The chromosomes line up in a The sister chromatids haploid (N) daughter cells, similar way to the metaphase separate and move toward each with half the number of stage of mitosis. opposite ends of the cell. chromosomes as the original. ...
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Vectors in gene therapy

Gene therapy utilizes the delivery of DNA into cells, which can be accomplished by several methods, summarized below. The two major classes of methods are those that use recombinant viruses (sometimes called biological nanoparticles or viral vectors) and those that use naked DNA or DNA complexes (non-viral methods).
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