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Fact Sheet 41 | CYSTIC FIBROSIS This fact sheet describes the
Fact Sheet 41 | CYSTIC FIBROSIS This fact sheet describes the

... To be affected with cystic fibrosis, both copies of the CFTR gene must be faulty. Without a working copy of the CFTR gene, the body is unable to produce the important salt-transport protein, resulting in the build-up of thick, sticky mucus characteristic of cystic fibrosis. The CFTR gene is a long i ...
Chapter 6: Gene Expression
Chapter 6: Gene Expression

... regions) are joined together. This process, called splicing, is performed by snRNA and snRNP proteins, which form a large spliceosome complex. In some cases, only certain exons are used to form a mature RNA, allowing for one gene to code for more than one protein. ...
CellFinder: a cell data repository
CellFinder: a cell data repository

... the number of different types ranging from 200 to 2260 (3,4). As ultimately any cell can be characterized by the molecules and processes it harbors at a given point in time, it is natural to base cell categorizations on the similarity of measured molecular properties of cells, like the state of the ...
Elective choices for a Biochemsitry Degree
Elective choices for a Biochemsitry Degree

... 2015 semester, the General Toxicology course has been revamped to focus on basic scientific literature relevant to toxicology and the scientific method that are of interest to graduate students. Scientific literature relevant to toxicology and the scientific method are emphasized in all topics co ...
Basic Theories for Introductory Biology
Basic Theories for Introductory Biology

... [This is a companion to “Promoting Creative and Critical Thinking Skills in College Biology”, by Anton E. Lawson, Department of Biology, Arizona State University, Tempe, AZ 85287-1501. Bioscene, vol. 27(1), March 2001. This document elaborates on Table 2 in that publication. Dr. Lawson kindly contri ...
Legends
Legends

... considered significant if the corrected p-value > 0.05. A gene is taken up in a list only if its expression is changed significantly in one direction in at least 4 out of 5 islet samples and in the other direction in none. An “existence score” is computed as the arithmetic mean of the natural logari ...
DNA ppt notes 2015
DNA ppt notes 2015

... Short Tandem Repeats (STR) STR is another method of DNA typing. STR’s are locations (loci) on the chromosome that contain short sequences of 2 to 5 bases that repeat themselves in the DNA molecule. The advantages of this method are that it provides greater discrimination, requires less time, a smal ...
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Genome-wide DNA replication profile for

... genes, however, reside in β-heterochromatin, which, unlike the centromeric α-heterochromatin, does not possess highly repetitive DNA and has a euchromatin-like gene density13. Thus, the various forms of D. melanogaster heterochromatin differ in several respects, including replication timing. Regions ...
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... color results from an α-fragment of β-galactosidase combining with an ω-fragment of β-galactosidase to form an active β-galactosidase molecule. When the α-fragment is inactivated by a cloned insert, no active β-galactosidase is made. ...
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1. dia - immunology.unideb.hu

... 9% of the Caucasian population is heterozygous for a deletion mutant of the CCR5 gene, which results in an unfunctional protein ...
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A prophage-encoded actin-like protein required for efficient viral

... virion assembly and lysis of the host cell (3,4). Recent reports revealed that viral replication in prokaryotes appears to be organized at specific intracellular locations and this process relies on the action of cytoskeletal proteins (5). Cytoskeletal elements in eu- and prokaryotes are involved in ...
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... Alleles: Alternative forms of a trait. Autosomes: All chromosomes with the exception of the sex chromosomes. Biology: The study of life. Carrier: An individual who has the allele for a trait or disease but does not have the disease or outwardly express the trait. Diploid: A cell that contains both c ...
The making of the Fittest: Natural Selection and
The making of the Fittest: Natural Selection and

... • Students may wonder why Pitx1 is expressed in such diverse tissues. This is because the Pitx1 gene contains multiple regulatory switches that allow for transcription of that gene in multiple tissues. The expression of Pitx1 is important in various tissues because the Pitx1 protein is itself a regu ...
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A Simple Mouthwash Method for Obtaining Genomic DNA in

... rinse) or do not yield an adequate amount (urine, hair roots, and saliva) or quality (paraffin blocks) of DNA. Also, some of these methods require the samples to be stored in a preservative solution that is toxic, which makes it problematic for use by mail (buccal brushes and swabs). This work was a ...
the cell cycle
the cell cycle

...  Some treatments for cancer involve the use of drugs that specifically attack cells that are actively dividing. Why would this be effective for fighting cancerous cells? ...
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I. DNA, Chromosomes, Chromatin, and Genes II. DNA

... 2. tRNA (transfer RNA)- each carries a specific amino acid; the tRNA anti-codon will pair up with its complementary mRNA codon. 3. When the 1st and 2nd amino acid is in place, the rRNA joins them by forming a____________________. As process continues, amino acid chain is formed until a stop codon. ...
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About DNA Ligase The term ligase comes from the latin ligare

... 1. Add 2 μL ligation buffer 10x and 3 μL T4 DNA ligase to the tube containing 10 μL lambda DNA/EcoR I digest without the loading dye. 2. Plance the tube in a 16 C ice water baath and incubate for 20 minutes. At this temperature the T4 DNA ligase catalyzes the ligation of more than 95% of the lambda ...
Transvection in 2012: Site-Specific Transgenes Reveal a
Transvection in 2012: Site-Specific Transgenes Reveal a

... ability of one transgene to activate the expression of another, greatly increasing our knowledge of trans-interactions and suggesting many experiments for the future. However, beyond that, their approaches to studying transvection and the questions they addressed differ. Bateman et al. (2012) used r ...
Genetic engineering of plants - HelhaPHL2010-05
Genetic engineering of plants - HelhaPHL2010-05

... Genetic engineering refers to all techniques that transfer genes from one organism to another, to produce new or modified organisms. The most used general technique is the recombinant DNAtechnique. There also plant specific techniques like transformation by A. tumefaciens. With these techniques, med ...
DNA in Action! A 3D Swarm-based Model of a Gene Regulatory
DNA in Action! A 3D Swarm-based Model of a Gene Regulatory

... Once RNA polymerase attaches to a DNA region, it starts scanning along the chain of nucleotide bases (codons). Transcription occurs once RNA polymerase has encountered a viable promoter region. Genes adjacent to the promoter region are transcribed into mRNA, represented as a twisted single-strand he ...
Experimental General. All the DNA manipulations and bacterial
Experimental General. All the DNA manipulations and bacterial

... final extension at 72 °C for 1 min. The DNA fragments were separated by 1.2% agarose gel electrophoresis and purified with QIAquick Gel Extraction Kit. After the second PCR, the amplified DNA fragment was digested with Asc I and Bam HI. The DNA fragment was purified as described above, and then liga ...
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Genes and Codes - Peter Godfrey

... something to that basic picture; it seeks to add a claim about the special nature of some kinds of genetic causation, and a theoretically important analogy between these genetic processes and processes involving symbols and messages in everyday life. Further, the idea of coding itself -- both in gen ...
Talk2.stat.methods
Talk2.stat.methods

... mimicking Darwin's natural selection ...
Document
Document

THE DNA OF CAENORHABDITIS ELEGANS HE small
THE DNA OF CAENORHABDITIS ELEGANS HE small

... content and the value derived from the study of renaturation. This may be taken as evidence that the unit genome (LAIRD 1971) in C. elegans is contained in the haploid set of chromatids and that the slowly renaturing sequences are represented uniquely in this genome. Our results are very similar to ...
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Vectors in gene therapy

Gene therapy utilizes the delivery of DNA into cells, which can be accomplished by several methods, summarized below. The two major classes of methods are those that use recombinant viruses (sometimes called biological nanoparticles or viral vectors) and those that use naked DNA or DNA complexes (non-viral methods).
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