Real-Time PCR Probe Design
... •Select dyes with excitation/emission maxima compatible with the excitation/detection ranges of the instrument. •Select the appropriate quencher for each dye •Select non-fluorescent quenchers (e.g. BHQs, Dabcyl) instead of ...
... •Select dyes with excitation/emission maxima compatible with the excitation/detection ranges of the instrument. •Select the appropriate quencher for each dye •Select non-fluorescent quenchers (e.g. BHQs, Dabcyl) instead of ...
View as PDF
... crRNA and tracrRNA) makes this system attractive for laboratory use. By combining the crRNA and tracrRNA into a synthetic single guide RNA (sgRNA), a further simplified two-component system can be used to introduce targeted double-stranded breaks in genomic DNA (1). Breaks activate repair through er ...
... crRNA and tracrRNA) makes this system attractive for laboratory use. By combining the crRNA and tracrRNA into a synthetic single guide RNA (sgRNA), a further simplified two-component system can be used to introduce targeted double-stranded breaks in genomic DNA (1). Breaks activate repair through er ...
Chapter 15
... host genome at random. If the insertion is within a gene, it can cause a loss of function mutation. The viral DNA can remain in the host genome and be passed from one generation to the next. It’s called an endogenous retrovirus. ...
... host genome at random. If the insertion is within a gene, it can cause a loss of function mutation. The viral DNA can remain in the host genome and be passed from one generation to the next. It’s called an endogenous retrovirus. ...
5.3 Meiosis - VCLivingEnvironment
... chromosomes line up with each other gene by gene along their length, to form a four-part structure called a tetrad. This is called SYNAPSIS. ...
... chromosomes line up with each other gene by gene along their length, to form a four-part structure called a tetrad. This is called SYNAPSIS. ...
Börjeson–Forssman–Lehmann syndrome: defining
... are ARHGEF6, RBMX, GPR101, ZIC3, FGF13, F9, and MCF2. The guanine nucleotide exchange factor ARHGEF6 maps at position 132 Mbp, which is just within the limits of our interval. Exon skipping in ARHGEF6 was reported to be responsible for the nonspecific X-linked mental retardation type 46 (MRX46; OMIM ...
... are ARHGEF6, RBMX, GPR101, ZIC3, FGF13, F9, and MCF2. The guanine nucleotide exchange factor ARHGEF6 maps at position 132 Mbp, which is just within the limits of our interval. Exon skipping in ARHGEF6 was reported to be responsible for the nonspecific X-linked mental retardation type 46 (MRX46; OMIM ...
Nuclear Gene Trees and the Phylogenetic Relationships of the
... Groves 1978). These phylogenies are derived from analyses of immunology, chromosome structure, amino acid sequences, and mtDNA sequences (Cronin and Sarich 1976; Hewett-Emmett, Cook, and Barnicot 1976; Dutrillaux, Fosse, and Chauvier 1979; Disotell 1994; Van der Kuyl et al. 1994). Despite the consis ...
... Groves 1978). These phylogenies are derived from analyses of immunology, chromosome structure, amino acid sequences, and mtDNA sequences (Cronin and Sarich 1976; Hewett-Emmett, Cook, and Barnicot 1976; Dutrillaux, Fosse, and Chauvier 1979; Disotell 1994; Van der Kuyl et al. 1994). Despite the consis ...
Why dread a bump on the head?
... 1. Garcia, J.H., Liu, K-F., Ye, Z-R, and Gutierrez, J.A. (1997). Incomplete infarct and delayed neuronal death after transient middle cerebral artery occlusion in rats. Stroke, 28: 23032310. 2. Li, Y., Chopp, M., Powers, C., and N. Jiang. (1997). Apoptosis and protein expression after focal cerebral ...
... 1. Garcia, J.H., Liu, K-F., Ye, Z-R, and Gutierrez, J.A. (1997). Incomplete infarct and delayed neuronal death after transient middle cerebral artery occlusion in rats. Stroke, 28: 23032310. 2. Li, Y., Chopp, M., Powers, C., and N. Jiang. (1997). Apoptosis and protein expression after focal cerebral ...
High School Biology MCAS Performance Level Descriptors
... Produces Punnett squares and calculates genotype and phenotype ratios for monohybrid crosses ...
... Produces Punnett squares and calculates genotype and phenotype ratios for monohybrid crosses ...
Extent of Gene Duplication in the Genomes of
... Gene family clustering is a difficult problem for two reasons. First, domain shuffling, which is a common mode for protein evolution, might mislead the clustering of two nonhomologous proteins into the same family because of the shared domain alone. Second, deciding whether two proteins are homologo ...
... Gene family clustering is a difficult problem for two reasons. First, domain shuffling, which is a common mode for protein evolution, might mislead the clustering of two nonhomologous proteins into the same family because of the shared domain alone. Second, deciding whether two proteins are homologo ...
Organs, Tissues and All Living Systems Long Answer Rubric
... A1. demonstrate scientific investigation skills (related to both inquiry and research) in the learning that plants and four areas of skills (initiating and planning, performing and recording, analysing and animals are made of interpreting, and communicating); specialized cells that form A2. identify ...
... A1. demonstrate scientific investigation skills (related to both inquiry and research) in the learning that plants and four areas of skills (initiating and planning, performing and recording, analysing and animals are made of interpreting, and communicating); specialized cells that form A2. identify ...
Imaging fertilization in flowering plants, not so
... corresponding to the AGI At5G01860 (Wang et al., 2010) and At5G40260 (Pagnussat et al., 2007) allow expression in all gametophytic cell types and are not active after fertilization. Such promoters can be used to test the impact of gene activity during gametophytic life and to label both the egg cell ...
... corresponding to the AGI At5G01860 (Wang et al., 2010) and At5G40260 (Pagnussat et al., 2007) allow expression in all gametophytic cell types and are not active after fertilization. Such promoters can be used to test the impact of gene activity during gametophytic life and to label both the egg cell ...
Organs, Tissues and All Living Systems Long Answer
... A1. demonstrate scientific investigation skills (related to both inquiry and research) in the learning that plants and four areas of skills (initiating and planning, performing and recording, analysing and animals are made of interpreting, and communicating); specialized cells that form A2. identify ...
... A1. demonstrate scientific investigation skills (related to both inquiry and research) in the learning that plants and four areas of skills (initiating and planning, performing and recording, analysing and animals are made of interpreting, and communicating); specialized cells that form A2. identify ...
The Chloroplast-Located Homolog of Bacterial DNA Recombinase
... Pstl/EcoRl fragment was excised from the genomic clone, precA-AT, and subcloned between Pstl and £coRI sites of pBSII SK. The resulting plasmid was digested with EcoRI/Xhol and religated with a 1.2 kb EcoKl/Xhol fragment excised from the cDNA clone, pcDNA recA3, to generate pJC-3. This plasmid conta ...
... Pstl/EcoRl fragment was excised from the genomic clone, precA-AT, and subcloned between Pstl and £coRI sites of pBSII SK. The resulting plasmid was digested with EcoRI/Xhol and religated with a 1.2 kb EcoKl/Xhol fragment excised from the cDNA clone, pcDNA recA3, to generate pJC-3. This plasmid conta ...
Differential effect of auxotrophies on the release of macromolecules
... already at 1 h (Fig. 2). Such bulges were expected as similar structures have been observed when bacteria were treated with antibiotics that also block cell wall synthesis (Bayer, 1967; Staugaard et al., 1976). At this early time point, spheres were observed that probably represent bacterial debris. ...
... already at 1 h (Fig. 2). Such bulges were expected as similar structures have been observed when bacteria were treated with antibiotics that also block cell wall synthesis (Bayer, 1967; Staugaard et al., 1976). At this early time point, spheres were observed that probably represent bacterial debris. ...
Evolution of cis-regulatory elements in duplicated genes of yeast
... gained but rather both genes are required to produce the full complement of functions of the ancestral gene. For the model to work, subfunctions need to be independent, hence most mutations should affect only one. Thanks to advances in molecular genetics, it has now become clear that many genes have ...
... gained but rather both genes are required to produce the full complement of functions of the ancestral gene. For the model to work, subfunctions need to be independent, hence most mutations should affect only one. Thanks to advances in molecular genetics, it has now become clear that many genes have ...
supplementary material
... effectively by taking into account two distinct types of genetic regulation: cis- and transregulation. In the case of cis-regulation, a cis-eQTL affects a particular etrait X and is located at the physical location of gene X on a chromosome. The polymorphism of a ciseQTL likely corresponds to a prom ...
... effectively by taking into account two distinct types of genetic regulation: cis- and transregulation. In the case of cis-regulation, a cis-eQTL affects a particular etrait X and is located at the physical location of gene X on a chromosome. The polymorphism of a ciseQTL likely corresponds to a prom ...
The polymerase chain reaction
... temperatures; a degenerate primer is used to amplify in the other direction from the unknown sequence. • Touchdown PCR (Step-down PCR): a variant of PCR that aims to reduce nonspecific background by gradually lowering the annealing temperature as PCR cycling progresses. The annealing temperature at ...
... temperatures; a degenerate primer is used to amplify in the other direction from the unknown sequence. • Touchdown PCR (Step-down PCR): a variant of PCR that aims to reduce nonspecific background by gradually lowering the annealing temperature as PCR cycling progresses. The annealing temperature at ...
Peer-reviewed Article PDF
... mainly in the liver, vectors derived from a recombinant retrovirus can efficiently transduce the PAH cDNA into PAH-deficient hepatocytes in vitro, but the transduction efficiency is low in vivo [18]. Beyond this barrier, studies have been initiated in which vectors derived from a recombinant adenovi ...
... mainly in the liver, vectors derived from a recombinant retrovirus can efficiently transduce the PAH cDNA into PAH-deficient hepatocytes in vitro, but the transduction efficiency is low in vivo [18]. Beyond this barrier, studies have been initiated in which vectors derived from a recombinant adenovi ...
Regulation of T Lymphocyte Metabolism
... (33). mTOR regulates protein synthesis via multiple phosphorylation targets, including p70S6 kinase, a regulator of ribosome function, and 4E-BP1, an inhibitor of translation (32). mTOR also directs the cell surface expression of a wide variety of nutrient transporters, including amino acid transpor ...
... (33). mTOR regulates protein synthesis via multiple phosphorylation targets, including p70S6 kinase, a regulator of ribosome function, and 4E-BP1, an inhibitor of translation (32). mTOR also directs the cell surface expression of a wide variety of nutrient transporters, including amino acid transpor ...
Sexual Reproduction: Meiosis
... Meiosis II: • Essentially a mitotic division of the products of Meiosis I that now separates the chromatids • Meiosis 2 is similar to mitosis. However, there is no "S" phase. The chromatids of each chromosome are no longer identical because of recombination. • Meiosis II separates the chromatids pr ...
... Meiosis II: • Essentially a mitotic division of the products of Meiosis I that now separates the chromatids • Meiosis 2 is similar to mitosis. However, there is no "S" phase. The chromatids of each chromosome are no longer identical because of recombination. • Meiosis II separates the chromatids pr ...
Biology 2672a
... • These cycles of breakdown and production occur on a circadian basis, providing the basis for the circadian rhythm ...
... • These cycles of breakdown and production occur on a circadian basis, providing the basis for the circadian rhythm ...
Life and Chemistry: Large Molecules
... • The discovery of ribozymes provided a solution to the question of whether proteins or nucleic acids came first when life originated. • Since RNA can be informational and catalytic, it could have acted as a catalyst for its own replication as well as for the synthesis of proteins. ...
... • The discovery of ribozymes provided a solution to the question of whether proteins or nucleic acids came first when life originated. • Since RNA can be informational and catalytic, it could have acted as a catalyst for its own replication as well as for the synthesis of proteins. ...
Exam - Vcaa
... Structure X represents a molecule of A. phospholipid. B. glycoprotein. C. carbohydrate. D. cholesterol. Question 7 During photosynthesis in chloroplasts, energy is used to split water, forming oxygen and hydrogen ions. The splitting of water occurs A. in the stroma during the light-independent react ...
... Structure X represents a molecule of A. phospholipid. B. glycoprotein. C. carbohydrate. D. cholesterol. Question 7 During photosynthesis in chloroplasts, energy is used to split water, forming oxygen and hydrogen ions. The splitting of water occurs A. in the stroma during the light-independent react ...
Topic B1
... Learners should be familiar with cells as the fundamental unit of living organisms and with the use of light microscopes to view cells. Activity 3 from the Cell Level Systems Delivery Guide here can be used as an introduction to scale and microscopy. Activity 1 from the Cell Level Systems Delivery G ...
... Learners should be familiar with cells as the fundamental unit of living organisms and with the use of light microscopes to view cells. Activity 3 from the Cell Level Systems Delivery Guide here can be used as an introduction to scale and microscopy. Activity 1 from the Cell Level Systems Delivery G ...