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Molecular analysis of extracellular-superoxide dismutase
Molecular analysis of extracellular-superoxide dismutase

... To study the qualitative heterogeneity of serum EC-SOD, we performed molecular analysis of the EC-SOD gene. The nucleotide sequence of human EC-SOD cDNA 002947 in EMBL database) has been reported by Hjalmarsson et al. (1987). Based on the sequence, 10 specific oligonucleotide primers were designed f ...
Rare variant discovery using family based studies / John Blangero
Rare variant discovery using family based studies / John Blangero

... •  SNP  located  within  exon  1  of  MOG   •  A  membrane  protein  expressed  on   the  oligodendrocyte  cell  surface   and  the  outermost  surface  of   myelin  sheaths   •  Primary  target  an
1_Genbank
1_Genbank

... Sequence databases are great tools because they offer a unique window on the past. They make it possible to answer today’s biological questions by enabling us to analyze sequences that may have been determined as many as 25 years ago, when the whole technology emerged. By doing this, they connect pa ...
Mitochondria, the cell cycle, and the origin of sex via a syncytial
Mitochondria, the cell cycle, and the origin of sex via a syncytial

... Many variations on this theme exist, for example, the presence or absence of the nuclear envelope in closed and open mitosis (Raikov 1994) or other variants such as cell senescence or specialization where the cells enter into a stage where they cease dividing (Blagosklonny 2011). Meiosis, also calle ...
File - Ms. Kuiper`s Website
File - Ms. Kuiper`s Website

... 19. The sequence of amino acids is called the _______________. The ___________________________ is often in the form of an alpha helix, which is due to __________________________ between amino acids in the chain. The ______________ __________________ is the three dimensional shape of the protein as i ...
bacteria - CNR WEB SITE
bacteria - CNR WEB SITE

... known as a nucleoid, but there is no membrane around the nucleoid, and the DNA is not bound to proteins and organized into linear pieces of chromosomes like in the eukaryotes. Bacterial DNA forms loops, called plasmids, that can be transmitted from one cell to another, either in the course of sex or ...
separation of single gene effects from additive
separation of single gene effects from additive

... (2) the model is extendable to other complicated genetic models. With increased detection and use of molecular markers, this method provides the opportunity to include cases with multiple linked or unlinked qualitative genes (or markers). If more generations are included, the model can also be exten ...
Student Edition Sample Chapter (3MB PDF)
Student Edition Sample Chapter (3MB PDF)

... Golgi bodies Golgi bodies receive proteins and other compounds from the ER. They package these materials and distribute them to other parts of the cell. They also release materials outside of the cell. The number and size of Golgi bodies found in a cell depends on the quantity of compounds produced ...
Brief Contents
Brief Contents

... Photosynthesis Reviewed and Extended 118 7.12 Review: Photosynthesis uses light energy, carbon dioxide, and water to make organic molecules 118 Connection Photosynthesis may moderate global climate change ...
Microarray expression data
Microarray expression data

... that is already correlated with survival data or disease-stage or disease-type, that can be applied to train the SVM to classify samples for cancer diagnostics, for example. In many cases samples look the same histologically, but their “expression fingerprint” is different. A certain “expression fin ...
Question paper - Unit F215/01 - Control, genomes and
Question paper - Unit F215/01 - Control, genomes and

... large enough. They are then dug up in Winter, the roots are cut into pieces and the pieces are replanted. Each piece is then able to grow into a new rhubarb plant that is identical to the parent. (i) ...
Full-Text PDF
Full-Text PDF

... beta-retrovirus. It is the result of a relatively recent integration, and conserves almost complete coding capacity; only a single stop codon is found in the env gene. As this stop codon is not seen in mRNA isolated from horse tissue, it might be a sequencing artifact. No other full-length EqERV-bet ...
Masters_Thesis_Final - JScholarship
Masters_Thesis_Final - JScholarship

... However, data from GWAS of over 240 traits and diseases, identifying over 3500 associated SNPs, shows that about 88% of these SNPs lie within non-coding region of the genome [5]. These non-coding variants are hypothesized to lie in regulatory regions of the genome, which regulate gene expression. So ...
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... 1.2.2 Function of the matK gene: maturase MatK The first putative function of the matK gene came from a sequence homology search through the GenBank, databases for DNA sequences. Neuhaus and Link (1987) found that a segment near the carboxyl terminus of the derived Sinapsis alba matK polypeptide was ...
forever young: a gene facilitating the study of the third larval instar of
forever young: a gene facilitating the study of the third larval instar of

... was homozygous lethal and had a sra phenotype in trans-heterozygous sra/sral1 or sra/sral2 animals. A genomic rescue with a construct containing the sra genomic DNA including the 5’ regulatory sequences was performed. This rescue construct reverted the sra phenotype (studied in trans-heterozygotes) ...
Exporter la page en pdf
Exporter la page en pdf

... replication timing profile shows a broad distribution of replication timing gradients with practically no regions larger than 100 kb replicating at less than 2 kb/min. Therefore, HeLa cells lack large regions of unidirectional fork progression. Temporal transition regions are replicated by sequential ...
DNA Shape Dominates Sequence Affinity in Nucleosome Formation
DNA Shape Dominates Sequence Affinity in Nucleosome Formation

... strong electrostatic attraction to the positively charged histone surface. Protein-bound sites along DNA present barriers to transcription; thus, their positioning is a crucial element in the regulation of cellular function for all eukaryotic species [1–3]. In spite of being central to biology, the ...
Cell wall-deficient, L-form bacteria in the 21st century: a personal
Cell wall-deficient, L-form bacteria in the 21st century: a personal

Production of industrially relevant compounds in prokaryotic
Production of industrially relevant compounds in prokaryotic

... is catechol. In some examples, the 3,4-DHB decarboxylase gene is from Klebsiella pneumoniae, Enlerobacler cloacae, Laclobacillus planlarum, or Closlridium bulryricum (for example, one of SEQ ID NOs: 4-11). [0009] In a further embodiment, the method includes expressing a heterologous asbF gene, a het ...
SCOOTER OER Fact Sheet: Dr V Rolfe, December 2010
SCOOTER OER Fact Sheet: Dr V Rolfe, December 2010

... How many chromosomes are there in a human cell? ...
What does PCR stand for?
What does PCR stand for?

... • Tt (PTC weak taster) • tt (PTC non-taster) ...
Ch5
Ch5

... “saved more lives by the introduction of his system than all the wars of the 19th century together had sacrificed.” • Lister revolutionized surgery: introduced methods to prevent infection of wounds • Impressed with Pasteur’s work, he wondered if ‘minute organisms’ might be responsible for infection ...
A Transient Expression Assay Using Maize Mesophyll Protoplasts
A Transient Expression Assay Using Maize Mesophyll Protoplasts

... In this system, photosynthetic gene promoters show tissue-specific and developmental (greening and normal green leaf development) regulation. In addition, regulated gene expression in response to metabolites, light, heat shock, phytohormone, redox, photooxidation, elicitor has been observed. Agents ...
Advanced primer design
Advanced primer design

... If only small number of primer sets is generated for GC rich or AT rich sequences, it is plausible that the primer design conditions for the given target sequence are too stringent. In PrimerExplorer V3, the primer design conditions are automatically selected for GC rich or AT rich sequences, but fo ...
Vertebrate DNA Transposon as a Natural Mutator
Vertebrate DNA Transposon as a Natural Mutator

... the mutations observed here are not ‘‘loss of function’’ or simple ‘‘gain of function’’ but rather ‘‘diversification of function.’’ Another important feature is that this situation was realized without any exogenous agents such as chemicals or radiation. At present, the trigger for the germ line tra ...
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Vectors in gene therapy

Gene therapy utilizes the delivery of DNA into cells, which can be accomplished by several methods, summarized below. The two major classes of methods are those that use recombinant viruses (sometimes called biological nanoparticles or viral vectors) and those that use naked DNA or DNA complexes (non-viral methods).
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