Index case of acute myeloid leukemia in a family
... Given the persistence of CEBPA c.442G.T at the time of CR, we postulated that it was a germ line mutation. We confirmed this by conducting Sanger sequencing of the CEBPA gene in genomic DNA isolated from skin fibroblasts (Figure 1A). This is a nonsense mutation located downstream of the p30 start co ...
... Given the persistence of CEBPA c.442G.T at the time of CR, we postulated that it was a germ line mutation. We confirmed this by conducting Sanger sequencing of the CEBPA gene in genomic DNA isolated from skin fibroblasts (Figure 1A). This is a nonsense mutation located downstream of the p30 start co ...
Use of the Polymerase Chain Reaction to Detect B
... rigorous testing of different vitrectomy solutions to determine how these would affect viability of the cells, and we could not test how long a specimen would survive before degradation of the DNA; this would also depend on how viable the cells were when removed from the patient. We did not think th ...
... rigorous testing of different vitrectomy solutions to determine how these would affect viability of the cells, and we could not test how long a specimen would survive before degradation of the DNA; this would also depend on how viable the cells were when removed from the patient. We did not think th ...
Gain of multiple copies of the CBFB gene: a new genetic
... leukemias. GS has been described in association with a variety of chromosomal abnormalities [6,7]. In particular, translocation t(8;21)(q22;q22) is regarded as a recurrent abnormality in most cases identified as AML M2 according to the FrencheAmericaneBritish (FAB) classification [1,8]. In contrast, ...
... leukemias. GS has been described in association with a variety of chromosomal abnormalities [6,7]. In particular, translocation t(8;21)(q22;q22) is regarded as a recurrent abnormality in most cases identified as AML M2 according to the FrencheAmericaneBritish (FAB) classification [1,8]. In contrast, ...
Caenorhabditis elegans unc-60 gene encodes
... unc-60 region, we examined genetically identified deftciencies of the region (Fig. 1) using PCR and primers derived from cosmid sequences (see Materials and methods). Deficiency homozygotes, arrested in development as embryos or early stage larvae, were used in the PCR amplifications (see Materials ...
... unc-60 region, we examined genetically identified deftciencies of the region (Fig. 1) using PCR and primers derived from cosmid sequences (see Materials and methods). Deficiency homozygotes, arrested in development as embryos or early stage larvae, were used in the PCR amplifications (see Materials ...
Base of questions of KROK-1 Exam Medical Biology 1. The cells of
... 65. During translation in the rough endoplasmic reticulum ribosome moves along the mRNA. Amino acids are linked together by peptide bonds in a specific sequence, and thus polypeptide synthesis takes place. The sequence of amino acids in a polypeptide corresponds to the sequence of: A. *mRNA codons B ...
... 65. During translation in the rough endoplasmic reticulum ribosome moves along the mRNA. Amino acids are linked together by peptide bonds in a specific sequence, and thus polypeptide synthesis takes place. The sequence of amino acids in a polypeptide corresponds to the sequence of: A. *mRNA codons B ...
Tutorial: chloroplast genomes - DOGMA: Annotation of Chloroplast
... 3. Create a userid. DOGMA is case-sensitive, and userids should not have any spaces or punctuation in them. 4. Create a password. DOGMA is case-sensitive, and password should not have any spaces or punctuation in them. This site does not use encryption so do not use an important password. 5. Enter y ...
... 3. Create a userid. DOGMA is case-sensitive, and userids should not have any spaces or punctuation in them. 4. Create a password. DOGMA is case-sensitive, and password should not have any spaces or punctuation in them. This site does not use encryption so do not use an important password. 5. Enter y ...
Torch 13.2 (April 2012)
... How did whole genome sequencing lead to the discovery of MYD88 L265P mutation in WM patients? Whole genome sequencing (WGS) is a powerful new technology that enables the reading of each of the 3 billion DNA molecules that make up the 23 paired chromosomes that are found in the nucleus of a human ce ...
... How did whole genome sequencing lead to the discovery of MYD88 L265P mutation in WM patients? Whole genome sequencing (WGS) is a powerful new technology that enables the reading of each of the 3 billion DNA molecules that make up the 23 paired chromosomes that are found in the nucleus of a human ce ...
In Sweden, Mexico, the United States, and a num
... that fetal tissue may be useful for treating patients with disabling diseases. For over 50 years, research in animals has demonstrated that fetal tissue has a unique capacity to replace certain cellular deficiencies and so may be useful for treating some chronic diseases of humans. These diseases in ...
... that fetal tissue may be useful for treating patients with disabling diseases. For over 50 years, research in animals has demonstrated that fetal tissue has a unique capacity to replace certain cellular deficiencies and so may be useful for treating some chronic diseases of humans. These diseases in ...
Complete Mitochondrial DNA Sequence and Amino Acid Analysis of
... AeCOI RNA expression levels were verified by RTPCR analysis on RNA from different developmental stages (pupal, larval and adult) and from female guts and carcasses (whole body minus gut). As seen in Fig. 4, no differential expression was observed and AeCOI is expressed at similar levels across all s ...
... AeCOI RNA expression levels were verified by RTPCR analysis on RNA from different developmental stages (pupal, larval and adult) and from female guts and carcasses (whole body minus gut). As seen in Fig. 4, no differential expression was observed and AeCOI is expressed at similar levels across all s ...
The replication of DNA
... placement of sliding camp on DNA. These enzyme couple ATP binding and hydrolysis to the placement of sliding clamp around primer template junction, every time that this junction is present in the cell. The clamp loaders also remove the slide clamp from DNA once all of the enzymes that interact with ...
... placement of sliding camp on DNA. These enzyme couple ATP binding and hydrolysis to the placement of sliding clamp around primer template junction, every time that this junction is present in the cell. The clamp loaders also remove the slide clamp from DNA once all of the enzymes that interact with ...
Assembly in G1 phase and long-term stability are unique
... is similar to the genome overall. This result, however, does demonstrate that the centromere is not a specialized chromatin domain that undergoes major nucleosome turnover events during G1 phase. Instead, CENP-A and H4 form a subnucleosomal core, which is specifically assembled at centromeres during ...
... is similar to the genome overall. This result, however, does demonstrate that the centromere is not a specialized chromatin domain that undergoes major nucleosome turnover events during G1 phase. Instead, CENP-A and H4 form a subnucleosomal core, which is specifically assembled at centromeres during ...
Quantitative Trait Loci and Comparative Genomics of Cereal Cell
... species, and a number of these have been successfully analyzed genetically. Because they are typically inherited in a quantitative manner, they are more challenging to analyze, and isolation of the responsible genes is more difficult. Nonetheless, in recent years a number of quantitative trait loci ...
... species, and a number of these have been successfully analyzed genetically. Because they are typically inherited in a quantitative manner, they are more challenging to analyze, and isolation of the responsible genes is more difficult. Nonetheless, in recent years a number of quantitative trait loci ...
Instructor Supplement: Ideas for Workshop Extension Activities Core
... These five proteins likely have very similar secondary and tertiary structures. These five proteins likely perform similar functions in the cell. The genes that encode these five proteins likely have a common evolutionary ancestor. A, B, and C are all correct. This is a remarkable coincidence, but y ...
... These five proteins likely have very similar secondary and tertiary structures. These five proteins likely perform similar functions in the cell. The genes that encode these five proteins likely have a common evolutionary ancestor. A, B, and C are all correct. This is a remarkable coincidence, but y ...
Biao Ding*, Myoung-Ok Kwon and Leif Warnberg
... kDa R-dextran(b). The photographwas taken 1 min after injection. (c and d) Thirty minutes after injection, the 20 kDa F-dextranwas clearlyvisible in ceils surrounding the injected cell (c), but the 40 kDa R-dextranstill remainedin the injected cell (d). The bar represents30 pm. ...
... kDa R-dextran(b). The photographwas taken 1 min after injection. (c and d) Thirty minutes after injection, the 20 kDa F-dextranwas clearlyvisible in ceils surrounding the injected cell (c), but the 40 kDa R-dextranstill remainedin the injected cell (d). The bar represents30 pm. ...
Gene Section MELK (maternal embryonic leucine zipper kinase) in Oncology and Haematology
... progression via the protein phosphatase CDC25B phosphorylation (Blot et al., 2002), in cytokinesis (Le Page et al., 2011), in apoptosis via its interaction with the Bcl-2 family of proapoptotic genes (Lin et al., 2007) and apoptosis signal-regulating kinase (ASK1) (Jung et al., 2008) and in inhibiti ...
... progression via the protein phosphatase CDC25B phosphorylation (Blot et al., 2002), in cytokinesis (Le Page et al., 2011), in apoptosis via its interaction with the Bcl-2 family of proapoptotic genes (Lin et al., 2007) and apoptosis signal-regulating kinase (ASK1) (Jung et al., 2008) and in inhibiti ...
pdf
... have been obtained that either increase or decrease its efficiency of initiating transcription. Base substitutions that make the promoter sequence more similar to the consensus generate a stronger promoter (promoter "up" mutations) whereas those that make the promoter less similar to the consensus g ...
... have been obtained that either increase or decrease its efficiency of initiating transcription. Base substitutions that make the promoter sequence more similar to the consensus generate a stronger promoter (promoter "up" mutations) whereas those that make the promoter less similar to the consensus g ...
Quasispecies evolution of a hypervariable region of the feline
... Following inoculation of cat Oscar with FCV, virus was isolated from the oropharynx up to 39 days p.i. Comparison of the 2 day p.i. virus (Fig. 1 b) with the infecting isolate (5th passage LS015, Fig. 1 a) showed they were very similar, the 2 day p.i. virus containing one synonymous nucleotide subst ...
... Following inoculation of cat Oscar with FCV, virus was isolated from the oropharynx up to 39 days p.i. Comparison of the 2 day p.i. virus (Fig. 1 b) with the infecting isolate (5th passage LS015, Fig. 1 a) showed they were very similar, the 2 day p.i. virus containing one synonymous nucleotide subst ...
Sickle Cell Disease
... birth, the screening test result for most normal infants is FA. Infants with hemoglobinopathies also show a predominance of Hb F at birth. Those with SCD show Hb S in absence of Hb A (FS), Hb S with another hemoglobin variant (e.g., FSC) or a quantity of Hb S greater than Hb A (FSA). In contrast, ca ...
... birth, the screening test result for most normal infants is FA. Infants with hemoglobinopathies also show a predominance of Hb F at birth. Those with SCD show Hb S in absence of Hb A (FS), Hb S with another hemoglobin variant (e.g., FSC) or a quantity of Hb S greater than Hb A (FSA). In contrast, ca ...
Full Text - BioTechniques
... spot). Spotting volume: 100 nL. Probe: M13 (the PCR-amplified G3PDH cDNA had a built-in M13 sequence). Detection: chemiluminescence. (B) A 1152-spot DNA membrane prepared by Hydra-PP 96. Gene: housekeeping gene β-actin. The spots in the first row contain 5 ng DNA each. The following rows are at 1/2, ...
... spot). Spotting volume: 100 nL. Probe: M13 (the PCR-amplified G3PDH cDNA had a built-in M13 sequence). Detection: chemiluminescence. (B) A 1152-spot DNA membrane prepared by Hydra-PP 96. Gene: housekeeping gene β-actin. The spots in the first row contain 5 ng DNA each. The following rows are at 1/2, ...
Cloning and characterisation of a cysteine proteinase gene
... amastigotes migrating around 30 kDa was completely inhibited after incubation of gelatin-coupled gels in the presence of E-64. 3.2. Cloning of a cysteine proteinase gene (Llacys1) from L. (L.) amazonensis By using genomic DNA of L. (L.) amazonensis amastigotes and a pair of primers derived from evol ...
... amastigotes migrating around 30 kDa was completely inhibited after incubation of gelatin-coupled gels in the presence of E-64. 3.2. Cloning of a cysteine proteinase gene (Llacys1) from L. (L.) amazonensis By using genomic DNA of L. (L.) amazonensis amastigotes and a pair of primers derived from evol ...
Genetics - Mount Mansfield Union High School
... • The genes are symbolized by the first letter of the dominant gene. • The letter for the dominant gene is always capitalized. • The letter for the recessive trait is always lower case (make sure you can tell the difference between the two) • Wild Type is the typical form of the organism, strain, or ...
... • The genes are symbolized by the first letter of the dominant gene. • The letter for the dominant gene is always capitalized. • The letter for the recessive trait is always lower case (make sure you can tell the difference between the two) • Wild Type is the typical form of the organism, strain, or ...