Control of human β-globin mRNA stability and its impact on beta
... Some experimental results suggested a role for the bglobin 3’UTR in the mRNA stability mechanism. Indeed, the replacement of the 3’ end of the c-fos mRNA (an unstable mRNA) with the 3’ end of the b-globin mRNA increases the half-life of the resultant hybrid mRNA, whereas the reciprocal hybrid transc ...
... Some experimental results suggested a role for the bglobin 3’UTR in the mRNA stability mechanism. Indeed, the replacement of the 3’ end of the c-fos mRNA (an unstable mRNA) with the 3’ end of the b-globin mRNA increases the half-life of the resultant hybrid mRNA, whereas the reciprocal hybrid transc ...
Whole-transcriptome RNAseq analysis from minute amount of total
... increased the transcript coverage, which has in turn enhanced our ability to detect novel rare transcripts, novel alternative splice isoforms and direct measurement of transcript abundance (3). These technologies are greatly accelerating our understanding of the complexity of gene expression, regula ...
... increased the transcript coverage, which has in turn enhanced our ability to detect novel rare transcripts, novel alternative splice isoforms and direct measurement of transcript abundance (3). These technologies are greatly accelerating our understanding of the complexity of gene expression, regula ...
FUNCTIONAL INVESTIGATION OF ARABIDOPSIS
... Callose synthesis occurs at specific stages of cell wall development in all cell types, and in response to pathogen attack, wounding and physiological stresses. We isolated promoters of 12 Arabidopsis callose synthase (CalS1-12) genes and demonstrated that different callose synthases are expressed s ...
... Callose synthesis occurs at specific stages of cell wall development in all cell types, and in response to pathogen attack, wounding and physiological stresses. We isolated promoters of 12 Arabidopsis callose synthase (CalS1-12) genes and demonstrated that different callose synthases are expressed s ...
Cloning and Polymorphisms of Yak Lactate Dehydrogenase b Gene
... LDH activity in heart and skeletal muscles has been reported in pikas from high altitudes when compared to pikas from low altitudes, which helps the pikas in high altitudes to improve anaerobic activity and to enhance lactate removal in muscles [4], since high LDH activity can catalyze more pyruvate ...
... LDH activity in heart and skeletal muscles has been reported in pikas from high altitudes when compared to pikas from low altitudes, which helps the pikas in high altitudes to improve anaerobic activity and to enhance lactate removal in muscles [4], since high LDH activity can catalyze more pyruvate ...
Shelef, Katie: A Critical Analysis of Degenerate Primer Design Programs
... CODEHOP (Rose et al 1998, 2003; Rose 2005) CODEHOP stands for “Consensus-degenerate hybrid oligonucleotide primer.” The CODEHOP program brings together two previous solutions for primer design for “distantly related” input sequences and combines them into one, “hybrid” primer [11]. One of these solu ...
... CODEHOP (Rose et al 1998, 2003; Rose 2005) CODEHOP stands for “Consensus-degenerate hybrid oligonucleotide primer.” The CODEHOP program brings together two previous solutions for primer design for “distantly related” input sequences and combines them into one, “hybrid” primer [11]. One of these solu ...
Regulators of Ubiquitin Dependent Protein Degradation in the
... function of the protein. Deletion of csnB leads, like deletion of the genes for other subunits of the CSN such as csnA, csnD or csnE, to a typical csn-deletion phenotype including red pigment formation and a block in sexual development. These results support the notion that all subunits of the compl ...
... function of the protein. Deletion of csnB leads, like deletion of the genes for other subunits of the CSN such as csnA, csnD or csnE, to a typical csn-deletion phenotype including red pigment formation and a block in sexual development. These results support the notion that all subunits of the compl ...
KingFisher Pure DNA Plant Kit
... of highly pure DNA from plant material using KingFisher magnetic particle processors. If the homogenization of the starting material is excluded, the approximate processing time is 40 minutes for the purification of 96 samples on the KingFisher Flex and 12 samples on the KingFisher Duo when the samp ...
... of highly pure DNA from plant material using KingFisher magnetic particle processors. If the homogenization of the starting material is excluded, the approximate processing time is 40 minutes for the purification of 96 samples on the KingFisher Flex and 12 samples on the KingFisher Duo when the samp ...
The amelogenin gene
... protein is now well characterized from the data of amino acid sequences which have been shown to be at a high degree of homology between all species investigated to date. The gene structure of this protein has been demonstrated and it is confirmed that there are two amelogenin genes, one on the X.ch ...
... protein is now well characterized from the data of amino acid sequences which have been shown to be at a high degree of homology between all species investigated to date. The gene structure of this protein has been demonstrated and it is confirmed that there are two amelogenin genes, one on the X.ch ...
Distribution and structural variation of the she pathogenicity island in
... A third putative virulence gene, pic, encoding a mucinase with haemagglutinin activity [7], is also carried on the PAI [1]. The complete nucleotide sequence and genetic organisation of the she PAI was determined recently and revealed that it has many of the features that are characteristic of PAIs f ...
... A third putative virulence gene, pic, encoding a mucinase with haemagglutinin activity [7], is also carried on the PAI [1]. The complete nucleotide sequence and genetic organisation of the she PAI was determined recently and revealed that it has many of the features that are characteristic of PAIs f ...
The Plasma Membrane - Beck-Shop
... Membrane–DNA Interactions: More Questions than Answers With the absence of internal membranes in prokaryotes, scientists have often speculated that the plasma membrane may function in directing some of the cellular processes. In 1963, F. Jacob and colleagues proposed the Replicon Model in which the ...
... Membrane–DNA Interactions: More Questions than Answers With the absence of internal membranes in prokaryotes, scientists have often speculated that the plasma membrane may function in directing some of the cellular processes. In 1963, F. Jacob and colleagues proposed the Replicon Model in which the ...
Inverse correlation between SMN1 and SMN2 copy numbers
... with two copies of SMN1.4,10 We meta-analyzed published data,3,4,8,10,13,14 and updated deduced SMN1 allele frequencies5 as follows: ‘zero-copy allele’ (chromosome 5 lacking SMN1 exon 7), 9.83 10 3; ‘one-copy allele’, 9.57 10 1; ‘two-copy allele’ (chromosome 5 with two copies of SMN1 exon 7), 3. ...
... with two copies of SMN1.4,10 We meta-analyzed published data,3,4,8,10,13,14 and updated deduced SMN1 allele frequencies5 as follows: ‘zero-copy allele’ (chromosome 5 lacking SMN1 exon 7), 9.83 10 3; ‘one-copy allele’, 9.57 10 1; ‘two-copy allele’ (chromosome 5 with two copies of SMN1 exon 7), 3. ...
::: Gene Set Enrichment Analysis - GSEA
... The Kolmogorov–Smirnov test is used to determine whether two underlying one-dimensional probability distributions differ, or whether an underlying probability distribution differs from a hypothesized distribution, in either case based on finite samples. The one-sample KS test compares the empirica ...
... The Kolmogorov–Smirnov test is used to determine whether two underlying one-dimensional probability distributions differ, or whether an underlying probability distribution differs from a hypothesized distribution, in either case based on finite samples. The one-sample KS test compares the empirica ...
Gekko 4_1 July 29
... gecko mutations discussed here. The next logical step would be to sequence likely candidate genes such as genes that code for tyrosinase, P protein, and tyrosinase-related-protein 1. The ability to find the affected locus and characterize the mutations at a molecular level would provide insight into ...
... gecko mutations discussed here. The next logical step would be to sequence likely candidate genes such as genes that code for tyrosinase, P protein, and tyrosinase-related-protein 1. The ability to find the affected locus and characterize the mutations at a molecular level would provide insight into ...
Taxonomic characterization of Ochrobactrum sp. isolates from soil
... from isolate 1a, SCII24T, OgA9aT, OiC8a, OiC8-6, LMG 5140, CLM14 and CLM18 was amplified by PCR using primers rD1 and fD1 (Weisburg et al., 1991). Amplificates of isolate 1a, SCII24T, OgA9aT, OiC8a and OiC8-6 were purified by low-melting agarose gel electrophoresis and sequenced following the dideox ...
... from isolate 1a, SCII24T, OgA9aT, OiC8a, OiC8-6, LMG 5140, CLM14 and CLM18 was amplified by PCR using primers rD1 and fD1 (Weisburg et al., 1991). Amplificates of isolate 1a, SCII24T, OgA9aT, OiC8a and OiC8-6 were purified by low-melting agarose gel electrophoresis and sequenced following the dideox ...
Expression and characterization of 1
... demonstrated that plants treated with ACCD containing P. putida UW4 (as opposed to a knockout mutant) display down-regulation of stress and defense genes but upregulation of growth-associated genes [17]. Despite the biological studies on the effects P. putida UW4 has on plant growth, little is known ...
... demonstrated that plants treated with ACCD containing P. putida UW4 (as opposed to a knockout mutant) display down-regulation of stress and defense genes but upregulation of growth-associated genes [17]. Despite the biological studies on the effects P. putida UW4 has on plant growth, little is known ...
Ana Maria da Silva Esteves Dissertation presented to obtain
... better suited for osmoprotection. This is one more illustration of solute replacement, a strategy generally used by organisms that rely on solute accumulation for osmoregulation. The patterns of compatible solute accumulation by Pyrococcus furiosus in response to heat stress are known, but the effec ...
... better suited for osmoprotection. This is one more illustration of solute replacement, a strategy generally used by organisms that rely on solute accumulation for osmoregulation. The patterns of compatible solute accumulation by Pyrococcus furiosus in response to heat stress are known, but the effec ...
Nucleic Acids Research
... deletion or site-directed mutagenesis analysis of these sequences. the 27 base changes that occur in this 240 nucleotide segment, most (17 of 27) are transitions which preserve a purine or pyrimidine, respectively, at the site of the change. Of the transversions that have occurred, most of these hav ...
... deletion or site-directed mutagenesis analysis of these sequences. the 27 base changes that occur in this 240 nucleotide segment, most (17 of 27) are transitions which preserve a purine or pyrimidine, respectively, at the site of the change. Of the transversions that have occurred, most of these hav ...
Basal Cell Carcinoma (Update 2012)
... BCC in Gorlin’s/ naevoid basal cell carcinoma syndrome (NBCCS) patients who have a germline mutation in the Patch 1 gene that encodes for the patched protein implicated in the patch sonic hedgehog pathway controlling embryonic development and cell proliferation in post natal life (20). Loss of the s ...
... BCC in Gorlin’s/ naevoid basal cell carcinoma syndrome (NBCCS) patients who have a germline mutation in the Patch 1 gene that encodes for the patched protein implicated in the patch sonic hedgehog pathway controlling embryonic development and cell proliferation in post natal life (20). Loss of the s ...
Sample
... 8. Workers in Dr. Garcia’s lab first obtain tumor cells from patients with cancer. They then remove the DNA from the tumor cell nuclei and use enzymes to segment the DNA strands into sections. Finally, they insert the DNA sections in which they are interested into “host” bacteria cells, where the DN ...
... 8. Workers in Dr. Garcia’s lab first obtain tumor cells from patients with cancer. They then remove the DNA from the tumor cell nuclei and use enzymes to segment the DNA strands into sections. Finally, they insert the DNA sections in which they are interested into “host” bacteria cells, where the DN ...
Pseudouridine at position 55 in tRNA controls the contents of other
... tRNA is produced by TruB. To clarify the role of the )55 modification, we constructed a truB gene disruptant ("truB) strain of Thermus thermophilus which is an extreme-thermophilic eubacterium. Unexpectedly, the "truB strain exhibited severe growth retardation at 50 C. We assumed that these phenome ...
... tRNA is produced by TruB. To clarify the role of the )55 modification, we constructed a truB gene disruptant ("truB) strain of Thermus thermophilus which is an extreme-thermophilic eubacterium. Unexpectedly, the "truB strain exhibited severe growth retardation at 50 C. We assumed that these phenome ...
Transcriptome analysis of the two unrelated fungal
... conducted recently with wild-type and production strains indicating a complex transcriptome regulation during fermentation in high yield strains [11]. Here, we addressed the question of whether β-lactam biosynthesis in both fungi has undergone parallel changes in global gene expression during strain ...
... conducted recently with wild-type and production strains indicating a complex transcriptome regulation during fermentation in high yield strains [11]. Here, we addressed the question of whether β-lactam biosynthesis in both fungi has undergone parallel changes in global gene expression during strain ...
Bacterial hemoglobins and flavohemoglobins: versatile proteins and
... primary use as a substrate and its secondary e¡ects on metabolism. Its use as a substrate allows cellular metabolism to work at optimal levels of substrate utilization and energy yield. Oxygen is required for the regulation of a variety of cellular functions, which are expressed in response to oxyge ...
... primary use as a substrate and its secondary e¡ects on metabolism. Its use as a substrate allows cellular metabolism to work at optimal levels of substrate utilization and energy yield. Oxygen is required for the regulation of a variety of cellular functions, which are expressed in response to oxyge ...
invited review
... The lack of a dominant non-DR3 binding sequence below VDR peak summits suggests that there is no single alternative protein partner of the receptor, but rather a larger set of proteins (Tuoresmäki et al. 2014). These VDR partnering proteins will have a cell-specific expression pattern and may explain ...
... The lack of a dominant non-DR3 binding sequence below VDR peak summits suggests that there is no single alternative protein partner of the receptor, but rather a larger set of proteins (Tuoresmäki et al. 2014). These VDR partnering proteins will have a cell-specific expression pattern and may explain ...
9.3 Polymers - Central Lyon CSD
... A polymer is a large molecule that forms when many smaller molecules are linked together by covalent bonds. • The smaller molecules that join together to form a polymer are monomers. • In some polymers, there is only one type of monomer. Other polymers have two or more kinds of monomers. ...
... A polymer is a large molecule that forms when many smaller molecules are linked together by covalent bonds. • The smaller molecules that join together to form a polymer are monomers. • In some polymers, there is only one type of monomer. Other polymers have two or more kinds of monomers. ...