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splicing.pdf
splicing.pdf

... Ways in which alternative splicing can be used This is just a preview, you will explore some of these different uses in more detail in the activity that follows. • In the example above, alternative splicing was used to include only one of several versions of an exon into a final protein product. Thi ...
Evolutionary dynamics of full genome content in Escherichia coli
Evolutionary dynamics of full genome content in Escherichia coli

... 1998) such that the evolutionary distance, as resolved by nucleotide divergence in homologous sequences, is also re¯ected in the total constellation of genes shared between strains and species. The relationship between phylogeny and gene content has also been observed for divergent bacterial taxa (F ...
Mbd3, a component of the NuRD co-repressor
Mbd3, a component of the NuRD co-repressor

... epiblast in terms of gene expression (Pelton et al., 2002). However, little is known of the differentiation events from 3.5 dpc ICM to early epiblast and late epiblast, owing to the small size and inaccessibility of relevant tissues. One of the striking differences between the pluripotent cell popul ...
Mutation, Mutagens, and DNA Repair
Mutation, Mutagens, and DNA Repair

... Organisms have evolved at least four processes for repairing UV damage in DNA: photoreactivation, excision, error-prone, and recombination repair. Depending on the type of organism and the nature of the UV damage, these processes may successfully repair damage, partially repair the damage and create ...


... answer using either glucose storage/release from glycogen, as regulated by hormones, or glycolysis or the TCA cycle, as regulated by energy sensing. Protein phosphorylation: Enzyme in pathways can be substrates for kinases. The phosphorylated form of the protein can be active or inactive. Dephosphor ...
Developmental, transcriptome, and genetic alterations associated
Developmental, transcriptome, and genetic alterations associated

... Seedless variants are preferred as grapevine (Vitis vinifera L.) cultivars used for table grapes and raisin production. Given the high heterozygosity of the grapevine genome (Laucou et al., 2011), varietal characteristics are maintained through asexual propagation and thus a grapevine cultivar consi ...
WheatNet: A genome-scale functional network for hexaploid bread
WheatNet: A genome-scale functional network for hexaploid bread

... genome-scale network, which facilitates the prediction of novel candidate genes for a trait, can be constructed. Network-based predictions have been useful in the model plant Arabidopsis thaliana (Lee et al., 2010). However, such a predictive gene network is not yet available for bread wheat, Tritic ...
Ch. 7 PowerPoint Notes
Ch. 7 PowerPoint Notes

... • One cell gets almost all of the cytoplasm – The other is called a polar body • Can divide again, but will not survive ...
The evolutionary mechanics of bacterial protein toxins
The evolutionary mechanics of bacterial protein toxins

...  When these factors are blocked, new proteins cannot be synthesized, which leads to cell death.  Toxins in this group are responsible for diphtheria and bacilliary dysentery, and contribute to Pseudomonas aeruginosa infection. ...
Type XVII collagen gene mutations in junctional epidermolysis
Type XVII collagen gene mutations in junctional epidermolysis

... In recent years data have accumulated that indicate that the spliceosome can be a target for attempting gene correction. The spliceosome of a cell is the site where small-nuclear ribonucleoproteins facilitate removal of introns from premtRNA (the so-called splicing reaction) and therefore control th ...
MUSCULAR DYSTROPHY, DUCHENNE TYPE
MUSCULAR DYSTROPHY, DUCHENNE TYPE

... • Dystrophin is a rod-shaped cytoplasmic protein, and a vital part of a protein complex that connects the cytoskeleton of a muscle fiber to the surrounding extracellular matrix through the cell membrane. • Dystrophin provides structural stability to the Dystroglycan complex (DGC), located on the ce ...
Infectious Bursal Disease Virus (IBDV) genesig
Infectious Bursal Disease Virus (IBDV) genesig

... material. Try reducing the amount of starting material and repeat the DNA/RNA extraction. 3. Failing to add the Internal Extraction Control RNA to your sample during the DNA/RNA extraction process can also lead to a reported result of “positive result, poor quality sample”. Ensure that this step has ...
A1114-CFS-SD1 - Food Standards Australia New Zealand
A1114-CFS-SD1 - Food Standards Australia New Zealand

... and 900 tonnes of otherwise-processed sweet corn (FAOSTAT3 2015). Corn product imports to Australia and New Zealand included 6,050 and 2,096 tonnes respectively of corn flour and 3,455 and 13 tonnes respectively of corn oil (FAOSTAT3 2015). Corn is a major source of crystalline fructose and high fru ...
Genetic Alteration Panels
Genetic Alteration Panels

... cancer. The BRAF gene is most frequently mutated at codon 600. For example, the BRAF V600E mutation has been found in more than 60% of melanomas, as well as 7 to 8% of other cancers. The BRAF Genetic Alteration Cell Panel (ATCC® No. TCP-1032™) is composed of eight selected human tumor cell lines fro ...
PCAN: phenotype consensus analysis to support
PCAN: phenotype consensus analysis to support

... candidate gene causes a particular disease phenotype under investigation (Fig. 1). Firstly, genes that are mechanistically related to the gene candidate are identified using a reference set of canonical pathways or a proteinprotein interaction network (step 1). PCAN also allows specification of cust ...
A Histone H3.3-like Gene Specifically Expressed in the Vegetative
A Histone H3.3-like Gene Specifically Expressed in the Vegetative

... Drastic changes in gene expression patterns occur during pollen development in flowering plants (Mascarenhas 1990, Becker et al. 2003, Honys and Twell 2003, McCormick 2004). In particular, recent microarray analyses in Arabidopsis have revealed unique characteristics of the pollen transcriptome (Bec ...
Characterization of Ubiquitin/Proteasome
Characterization of Ubiquitin/Proteasome

... For continuation of glycolysis, NAD+ must be regenerated through oxidation of the NADH produced by glycolysis. S. cerevisiae utilize alcoholic fermentation, in which pyruvate is converted to ethanol and carbon dioxide for the regeneration of NAD+ (Morton, 1980). This occurs in a two-step reaction wh ...
Garrett-Engele* C. M., Siegal*, M. L., Manoli, D. S., Williams, B. C., Li. H., and Baker, B. S.
Garrett-Engele* C. M., Siegal*, M. L., Manoli, D. S., Williams, B. C., Li. H., and Baker, B. S.

... length polymorphism (RFLP) mapping. A clone containing the ix gene was identified by its ability to rescue ix mutant phenotypes when introduced into flies by P-element-mediated germline transformation. The ix protein has sequence similarity to proteins proposed to act as transcriptional activators, ...
Lesson 1: Do you have good taste?
Lesson 1: Do you have good taste?

... between DNA and protein?  (Hint: Cells can make more than one mRNA copy of a gene and  more than one protein molecule from an mRNA.)   There are actually many valid answers to this ques on; however the accompanying hint  tries to steer student thinking toward one that doesn’t require extensive bac ...
GCAT-SEEK Workshop - Prokaryotic Genomics Module – Jeff
GCAT-SEEK Workshop - Prokaryotic Genomics Module – Jeff

... libraries and sequence the DNA using NextGen technologies, probably MiSeq or HiSeq, to 100x coverage.(steps 1-3 above). We will then use example data to learn how to assemble the sequences into contigs, with or without a reference, manually edit the sequence to identify more overlaps and gaps that a ...
Escherichia coli synthetic genetic array
Escherichia coli synthetic genetic array

... strain, with a drug-resistance marker replacing a gene of interest, is ...
Sequence Analysis of the DNA Encoding the Eco RI Endonuclease
Sequence Analysis of the DNA Encoding the Eco RI Endonuclease

... In some cases, frequently selected codons are recogIn the accompanying paper, Newman et al. (10) report a nized by minor tRNA species. A spontaneous mutation sequence analysis of the Eco RI genes contained in pMB4. a in the endonuclease gene was isolated. Serine replaces derivative of pMBl which det ...
- Haematopoietic Stem Cell Lab
- Haematopoietic Stem Cell Lab

... De novo motif discovery on Runx1 peaks from all 3 populations analysed by ChIP-Seq. In addition to Runx1 and Gata consensus motifs, a novel motif (unknown 1) was identified both as a shorter 15 bp motif and also embedded in a longer 27 bp motif (both the motif and its reverse complement were found b ...
Antibiotic Resistance and Genetically Engineered Plants
Antibiotic Resistance and Genetically Engineered Plants

... The process of inserting a gene of interest into a plant is crude, haphazard, and random. Scientists cannot easily determine where a gene will land, or even if a gene has been successfully incorporated into a plant cell. There are two common methods of gene insertion. The first involves a “gene gun ...
WHRHS BIOLOGY K PROFICIENCIES
WHRHS BIOLOGY K PROFICIENCIES

... 89. Describe the system of binomial nomenclature. 90. List the categories of the classification system used today. 91. State the name of each of the 6 kingdoms and list characteristics for each. 92. Classify organisms into their kingdom. 93. State the complete classification of humans. 94. Be able t ...
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Vectors in gene therapy

Gene therapy utilizes the delivery of DNA into cells, which can be accomplished by several methods, summarized below. The two major classes of methods are those that use recombinant viruses (sometimes called biological nanoparticles or viral vectors) and those that use naked DNA or DNA complexes (non-viral methods).
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