BLSSpeller: exhaustive comparative discovery of
... (osa), Brachypodium distachyon (bdi), Sorghum bicolor (sbi) and Zea mays (zma). We adopt a gene-centric approach, where the promoter sequences of orthologous genes are grouped into gene families. A word-based discovery algorithm was designed to exhaustively report all genome-wide conserved motifs. T ...
... (osa), Brachypodium distachyon (bdi), Sorghum bicolor (sbi) and Zea mays (zma). We adopt a gene-centric approach, where the promoter sequences of orthologous genes are grouped into gene families. A word-based discovery algorithm was designed to exhaustively report all genome-wide conserved motifs. T ...
Requirements for Driving Antipathogen Effector Genes into
... ABSTRACT There is a need for new interventions against the ongoing burden of vector-borne diseases such as malaria and dengue. One suggestion has been to develop genes encoding effector molecules that block parasite development within the vector, and then use the nuclease-based homing reaction as a ...
... ABSTRACT There is a need for new interventions against the ongoing burden of vector-borne diseases such as malaria and dengue. One suggestion has been to develop genes encoding effector molecules that block parasite development within the vector, and then use the nuclease-based homing reaction as a ...
New Construct Approaches for Efficient Gene Silencing in Plants
... double-transformed tobacco plants displayed a reduced level of gus expression (Table I). The stability of pSIM717-mediated gene silencing was assessed by assaying groups of approximately 25 T1 and T2 plants derived from three randomly chosen double transformants that segregated for single loci carry ...
... double-transformed tobacco plants displayed a reduced level of gus expression (Table I). The stability of pSIM717-mediated gene silencing was assessed by assaying groups of approximately 25 T1 and T2 plants derived from three randomly chosen double transformants that segregated for single loci carry ...
Clamp loader structure predicts the architecture of DNA polymerase
... accessible to β may be one in which the amino-terminal domain of δ′ interacts more extensively with the aminoterminal, β-interactive, domain of δ. Given very few modeling operations (explained in [17]) a hypothetical ‘closed’ structure can be formed in which the amino-terminal domains of all five su ...
... accessible to β may be one in which the amino-terminal domain of δ′ interacts more extensively with the aminoterminal, β-interactive, domain of δ. Given very few modeling operations (explained in [17]) a hypothetical ‘closed’ structure can be formed in which the amino-terminal domains of all five su ...
Copy Number and Gene Expression Integration in Partek
... samples with some samples paired tumor-normal. The first spreadsheet, Cifola_U133_2 contains the imported and summarized log2 transformed gene intensities. A two factor ANOVA was run including both disease state and individual. Then a list of 710 genes that were differentially expressed with respect ...
... samples with some samples paired tumor-normal. The first spreadsheet, Cifola_U133_2 contains the imported and summarized log2 transformed gene intensities. A two factor ANOVA was run including both disease state and individual. Then a list of 710 genes that were differentially expressed with respect ...
Enzyme Mechanisms - Illinois Institute of Technology
... Cross-shaped structures arise from palindromic structures, including interrupted palindromes like this example These are less stable than regular duplexes but they are common, and they do create recognition sites for DNA-binding proteins, including restriction enzymes ...
... Cross-shaped structures arise from palindromic structures, including interrupted palindromes like this example These are less stable than regular duplexes but they are common, and they do create recognition sites for DNA-binding proteins, including restriction enzymes ...
Use of a novel cassette to label phenotypically a cryptic plasmid of
... replication A restriction map of pPOD2000 is shown in Fig. 2, using coordinates originating at the unique SalI site. Previous attempts to insert a selectable HindIII fragment into the HindIII sites of pPOD2000 gave insertions at all sites except that at coordinate 2.2 kb, suggesting that this site l ...
... replication A restriction map of pPOD2000 is shown in Fig. 2, using coordinates originating at the unique SalI site. Previous attempts to insert a selectable HindIII fragment into the HindIII sites of pPOD2000 gave insertions at all sites except that at coordinate 2.2 kb, suggesting that this site l ...
WP4 - Tomsett
... are expressed in most tissues examined. The cytosolic CSase is root specific. Expression for the putative plastidic CSase is uniformly low. ...
... are expressed in most tissues examined. The cytosolic CSase is root specific. Expression for the putative plastidic CSase is uniformly low. ...
APC2 Antibody
... promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take ...
... promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take ...
Antioxidant and Xenobiotic-metabolizing Enzyme Gene Expression
... multifunctional molecule, with several possible mechanisms of activity could diminish doxorubicin sensitivity (17). Carbonyl cytotoxicity (1-5). Therefore, it is not unreasonable to expect reducÃ-ase activily can be delecled in leukemic blasts from that tumor cell resistance to doxorubicin may be co ...
... multifunctional molecule, with several possible mechanisms of activity could diminish doxorubicin sensitivity (17). Carbonyl cytotoxicity (1-5). Therefore, it is not unreasonable to expect reducÃ-ase activily can be delecled in leukemic blasts from that tumor cell resistance to doxorubicin may be co ...
Viral Encephalitis I. General Principles
... leukoencephalopathy (PML) caused by JC virus, subacute sclerosing panencephalitis (SSPE) occurring after measles, and HIV encephalopathy. E. Diagnosis of Viral Encephalitis Diagnosis begins with a thorough and detailed history which seeks to identify exposures by inquiring about insect or animal bit ...
... leukoencephalopathy (PML) caused by JC virus, subacute sclerosing panencephalitis (SSPE) occurring after measles, and HIV encephalopathy. E. Diagnosis of Viral Encephalitis Diagnosis begins with a thorough and detailed history which seeks to identify exposures by inquiring about insect or animal bit ...
Gene Expression Programming: A New Adaptive
... using a five-bit binary code, called genetic code. Contrary to its analogous natural genetic code, this “genetic code,” when applied to binary strings, frequently produces invalid expressions (in nature there is no such thing as an invalid protein). Therefore a huge amount of computational resources ...
... using a five-bit binary code, called genetic code. Contrary to its analogous natural genetic code, this “genetic code,” when applied to binary strings, frequently produces invalid expressions (in nature there is no such thing as an invalid protein). Therefore a huge amount of computational resources ...
Triple-hit lymphoma
... follicular lymphoma or BL, respectively. However, to have two gene rearrangements (double-hit lymphomas) is uncommon (2). Translocation of the BCL-2 gene on chromosome 18q21 results in constant inactivation of apoptosis (3). Translocation of the c-MYC gene on chromosome 8q24 results in constant cell ...
... follicular lymphoma or BL, respectively. However, to have two gene rearrangements (double-hit lymphomas) is uncommon (2). Translocation of the BCL-2 gene on chromosome 18q21 results in constant inactivation of apoptosis (3). Translocation of the c-MYC gene on chromosome 8q24 results in constant cell ...
Development and Optimization of a DNA extraction
... genetically modified organisms (GMOs) has increased significantly (Prado, et al., 2015), mostly due to the high stability and durability of DNA when compared with proteins. Moreover, DNA is species-specific, allowing the verification of species, and can be found in every cell of plants and animals w ...
... genetically modified organisms (GMOs) has increased significantly (Prado, et al., 2015), mostly due to the high stability and durability of DNA when compared with proteins. Moreover, DNA is species-specific, allowing the verification of species, and can be found in every cell of plants and animals w ...
High mutation rates in human and ape pseudoautosomal genes
... another human pseudoautosomal gene, SHOX, is not higher than elsewhere in the genome (May et al., 2002). A noncoding pseudoautosomal region close to the Xp/Yp telomere was reported to have a high substitution rate (Cooke et al., 1985; Baird and Royle, 1997), however, subtelomeric regions are known t ...
... another human pseudoautosomal gene, SHOX, is not higher than elsewhere in the genome (May et al., 2002). A noncoding pseudoautosomal region close to the Xp/Yp telomere was reported to have a high substitution rate (Cooke et al., 1985; Baird and Royle, 1997), however, subtelomeric regions are known t ...
Arabidopsis Contains Ancient Classes of Differentially Expressed
... Chua, 1999) examining both transcripts and promoter-driven reporter expression. The AtARP2 gene was expressed in only a small subset of vascular tissue types and pollen, and unlike any conventional actin, AtARP2 was expressed at very low levels. Thus, it seemed possible that AtARP2 and other subclas ...
... Chua, 1999) examining both transcripts and promoter-driven reporter expression. The AtARP2 gene was expressed in only a small subset of vascular tissue types and pollen, and unlike any conventional actin, AtARP2 was expressed at very low levels. Thus, it seemed possible that AtARP2 and other subclas ...
Primary ciliary dyskinesia: genes, candidate genes
... would be released on the left side of the node, where the flow remains the strongest. Data supporting the ciliary fluid flow hypothesis come from the studies of murine inversus viscerum (iv) mutants, harboring mutations in a ciliary component, the left-right dynein (the protein product of lrd) (Lowe ...
... would be released on the left side of the node, where the flow remains the strongest. Data supporting the ciliary fluid flow hypothesis come from the studies of murine inversus viscerum (iv) mutants, harboring mutations in a ciliary component, the left-right dynein (the protein product of lrd) (Lowe ...
PDF
... positive for the wild-type Ppp1r15b allele (P ⫽ 8.9 ⫻ 10⫺16), indicating that embryos lacking all PPP1R15 function failed to form a blastocyst cavity, grow, or hatch from the zona pellucida, and do not develop past the preimplantation period (Fig. 5B). To estimate the role of defective eIF2␣ dephosp ...
... positive for the wild-type Ppp1r15b allele (P ⫽ 8.9 ⫻ 10⫺16), indicating that embryos lacking all PPP1R15 function failed to form a blastocyst cavity, grow, or hatch from the zona pellucida, and do not develop past the preimplantation period (Fig. 5B). To estimate the role of defective eIF2␣ dephosp ...
NIH Guidelines - Institutional Biosafety Committee
... • III-F-1: Involving synthetic nucleic acids that: (1) can neither replicate nor generate nucleic acids that can replicate in any living cell (e.g., oligonucleotides or other synthetic nucleic acids that do not contain an origin of replication or contain elements known to interact with either DNA or ...
... • III-F-1: Involving synthetic nucleic acids that: (1) can neither replicate nor generate nucleic acids that can replicate in any living cell (e.g., oligonucleotides or other synthetic nucleic acids that do not contain an origin of replication or contain elements known to interact with either DNA or ...
Molecular Diagnostics as an Indispensable Tool for the
... dengue virus isolation usually takes several days. Four methods of viral isolation have been routinely used for dengue viruses: intracerebral inoculation of newborn mice, inoculation on mammalian cell cultures, intrathoracic inoculation of adult mosquitoes, and inoculation on mosquito cell cultures ...
... dengue virus isolation usually takes several days. Four methods of viral isolation have been routinely used for dengue viruses: intracerebral inoculation of newborn mice, inoculation on mammalian cell cultures, intrathoracic inoculation of adult mosquitoes, and inoculation on mosquito cell cultures ...
It`s All In The Genes - American Maine
... from this mating have a 50% chance of being heterozygous black (Bb - a black calf with one black and one red allele) and a 50% chance of being born red (bb). For the recessive gene to be displayed in the phenotype (i.e. for you to see it), the calf must inherit this gene from both parents. The same ...
... from this mating have a 50% chance of being heterozygous black (Bb - a black calf with one black and one red allele) and a 50% chance of being born red (bb). For the recessive gene to be displayed in the phenotype (i.e. for you to see it), the calf must inherit this gene from both parents. The same ...
CentrosomeDB: a new generation of the
... resource for the scientific community. This was our first motivation to introduce CentrosomeDB in NAR database issue in 2009, collecting a set of human centrosomal proteins that were reported in the literature and other sources. The intensive use of this resource during these years has encouraged us ...
... resource for the scientific community. This was our first motivation to introduce CentrosomeDB in NAR database issue in 2009, collecting a set of human centrosomal proteins that were reported in the literature and other sources. The intensive use of this resource during these years has encouraged us ...